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Offlineguyute22
Ugly Pig


Registered: 07/27/15
Posts: 504
Last seen: 5 years, 6 months
Pinning Plates
    #22504201 - 11/10/15 09:24 AM (8 years, 2 months ago)

I have two strains of cubes I am working with.  Mexican and AA+.  Both were started from multispore syringes to pastyplates, and isolated from there.

I have clean isolates of both, but the Mexican will pin on the agar as soon as it is fully colonized, where the AA+ has yet to ever pin.  I have a couple of plates that over a month old.

Does the fact that the AA+ won't pin on Agar mean it will not fruit, or be a poor producer when grown on oats, spawned to CVG? 

I will probably drop both to oats this weekend anyway, just wondering if anyone has any thoughts/experience with strains that won't pin on agar.


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Invisiblewowimflabbergasted
supercalifragilistic
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Registered: 07/16/12
Posts: 18,918
Re: Pinning Plates [Re: guyute22]
    #22504230 - 11/10/15 09:40 AM (8 years, 2 months ago)

No. I've never had anything pin on agar. Some people get pins all the time. I think it just indicates a faster fruiting culture not a non-fruiting one.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Pinning Plates [Re: wowimflabbergasted]
    #22505241 - 11/10/15 02:02 PM (8 years, 2 months ago)

I've never had an agar plate pin either, and it's been left out for about a month or so.


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Offlineguyute22
Ugly Pig


Registered: 07/27/15
Posts: 504
Last seen: 5 years, 6 months
Re: Pinning Plates [Re: Psilosoulful]
    #22506841 - 11/10/15 08:05 PM (8 years, 2 months ago)

So you guys are still having success with cultures that don't pin on agar?  That puts me at ease.

Thanks!


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Pinning Plates [Re: guyute22]
    #22506867 - 11/10/15 08:09 PM (8 years, 2 months ago)

:kenthumbup:


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