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OfflineEnergyTurtle
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: taGyo]
    #22671241 - 12/18/15 09:48 AM (8 years, 1 month ago)

Interesting idea, I'm gonna go for it and see what happens. I'm thinking of doing a single cake mono with some coir and verm, so if it contams before full colonization I can just pitch the whole micro-tub. Should I dunk the cake for a while before I spawn it? Is that even necessary when spawning to a mono?

EDIT: Never mind, found the answer to that with a bit of searching. Since the bulk is going to be hydrated to field capacity, dunking the cake would throw off the moisture balance. A lot of people say that BRF colonizes the bulk faster without a dunk. If the moisture of the bulk is higher than the moisture in the spawn, then I would imagine that the mycel would be more inclined to move to the bulk and colonize it.


--------------------
"You decadent pig. Where the fuck do you get the nerve to go around telling those wops that I'm crazy? You worthless cocksucker. My Italian tour is already arranged for next spring & I'm going to do the whole goddamn trip wearing a bright red field marshal's uniform & accompanied by six speed-freak bodyguards bristling with Mace bombs & when I start talking about American writers & the name Tom Wolfe comes up, by god, you're going to wish you were born a fucking iguana!" - Hunter S. Thompson, 03/03/71 Letter to Tom Wolfe.


Edited by EnergyTurtle (12/18/15 10:10 AM)


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OfflinetaGyo
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: EnergyTurtle]
    #22671309 - 12/18/15 10:12 AM (8 years, 1 month ago)

Yup.

Sounds about right. I just cased a bacterial tub full of B+ I think? The pinset is gonna be epic


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Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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InvisibleMad Season
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: taGyo]
    #22671341 - 12/18/15 10:26 AM (8 years, 1 month ago)

Certain species of mushrooms will only thrive as long as there's a type of bacteria present. I have been speculating that as well gyo, as of late. We have no clue just how much certain bacteria and microbes benefit cubes.. IMO it seems like they benefit quite a bit.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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Edited by Mad Season (12/18/15 10:26 AM)


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InvisibleNumeroEno
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: Mad Season]
    #22671430 - 12/18/15 10:50 AM (8 years, 1 month ago)

Bacteria is everywhere. Bacillus subtilis endospores can go right through a 99.99% hepa filter.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleMad Season
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: NumeroEno]
    #22671497 - 12/18/15 11:12 AM (8 years, 1 month ago)

Ikr! They literally make or break us :lol:

Never knew I'd have a fascination with this shit before getting into shrooms lol!


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblestareatclouds
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: Mad Season]
    #22671501 - 12/18/15 11:14 AM (8 years, 1 month ago)

I thought it was well accepted that certain bacteria, bad bacteria I mean, speeds up fruiting/colonizing. Not because it "helps" but because the myc is like, fuck, this is bad, time to hurry up.


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OfflinetaGyo
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: stareatclouds]
    #22671602 - 12/18/15 11:45 AM (8 years, 1 month ago)

Well I'm talking about a fully colonized tub that I consolidated until knots and then fruited. If I get a tub that doesn't even colonize the top layer of coir and fruits I know its bad.

When I'm talking about benefiting the myc I'm talking about tubs I've observed with known bacteria outcolonize their peers from the same master but a different round of g2g.

In this case it fruited twice heavy and then I saw trich. In other cases it fruits once heavily and then dead spots occur that generally start to contam over time. My fully colonized, clean tubs always outperform in the long run but the first two flushes of slightly bacterial spawn are enough to fill a gallon and two quart Ziploc bag and I use 27qt monos and 3 qts.

If I took some of these cultures further I'd need a warehouse :lol:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineMrSpadoodles
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: taGyo]
    #22672450 - 12/18/15 03:22 PM (8 years, 1 month ago)

okay so I've sterilized all my jars (hopefully it worked well).
where should I inoculate?
I don't have a glovebox but I do have a large tote I was gonna turn sideways to shield from wind. was gonna use a bic lighter to sterilize the syringe before each jar and I also have rubbing alcohol and latex gloves. would a kitchen counter be a good spot or is the kitchen too filled with contams? I imagine my bedroom and bathroom would also have a lot.. and so would doing it outside. I've got a small luggage room connected to the bathroom (separated by a door). it would be tight in the air but would that be a good spot? I heard about people spraying 10% bleach solutions in rooms.. would that ruin the walls, paint, etc.?
how do I put the syringe together without accidentally contaminating it?


--------------------
The meaning of life is just to be alive. It is so plain and so obvious and so simple. And yet, everybody rushes around in a great panic as if it were necessary to achieve something beyond themselves.
- Allan Watts


Looking for a P. Galindoi ATL#7 spore print/syringe. PM me to trade :pm:



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OfflineNDStepp84
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: MrSpadoodles]
    #22672497 - 12/18/15 03:31 PM (8 years, 1 month ago)

Cut armholes in that tote, bam! Still air box.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineMrSpadoodles
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: NDStepp84]
    #22672606 - 12/18/15 03:55 PM (8 years, 1 month ago)

I read somewhere a SAB isn't that important, and I need that tote for my FC. I also don't have tools to cut proper armholes


--------------------
The meaning of life is just to be alive. It is so plain and so obvious and so simple. And yet, everybody rushes around in a great panic as if it were necessary to achieve something beyond themselves.
- Allan Watts


Looking for a P. Galindoi ATL#7 spore print/syringe. PM me to trade :pm:



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OfflineNDStepp84
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: MrSpadoodles]
    #22672686 - 12/18/15 04:16 PM (8 years, 1 month ago)

Quote:

MrSpadoodles said:
I read somewhere a SAB isn't that important


  :header:
You would have a couple weeks while it colonized to save up 4 bucks for a new tote. You can be careful and use a knife, or heat up a metal can on the stove or something to cut the holes.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflinetaGyo
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: MrSpadoodles]
    #22672689 - 12/18/15 04:17 PM (8 years, 1 month ago)

SAB is super important :confused2:

I couldn't grow mushrooms as well as I do without it. For PF cakes I just inoculate open air. That's a bad practice though if you actually want success with them. I'd prop up a box somewhere, spray it down real good and at least do the inoculation in a semi-sealed environment.

Make sure to watch the mushroom vidoes DIRECTLY BEFORE you inoculate. this will keep you from making any stupid mistakes.

Worth noting I agree with ND, you should just make one because you're gonna need it.



--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Invisibledankington
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: MrSpadoodles]
    #22673313 - 12/18/15 07:01 PM (8 years, 1 month ago)

Quote:

MrSpadoodles said:
I read somewhere a SAB isn't that important, and I need that tote for my FC. I also don't have tools to cut proper armholes




Many have had success by properly cleaning the area and tote, and leaving it hanging over the edge of a table (enough for your arms to fit under the lip), and get to work. Of course, your posture/positioning this way will give you FLBP

:jiggly:

jk I'm an idiot. But not about the SAB. Please use some sort of SAB! I'm begging you to wait until you can.


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OfflineMrSpadoodles
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: dankington]
    #22673507 - 12/18/15 07:57 PM (8 years, 1 month ago)

too late lol I already inoculated without a SAB. the plastic seems to have been too thick to make any holes in so I just wiped it down well with alcohol, sprayed it with water, and put it on its side. I also cleaned the table and gloves and took a brief shower right beforehand. I throughly flame-sterilized the needle between jars and worked as quickly as I could without moving the jars around too much. I then cleaned the spot on my closet shelf with alcohol as well and sat the jars there carefully



two things: I noticed several of the jars were kinda damp on the spots where the tape is covering the inoculation holes. I'm thinking it's either condensation or the water from the pot boiled too hard and bipassed the aluminum cover. should I be concerned?
and the other thing.. I forgot to mark the holes on the one with the tyvek on the lid so I ended up having to do an open-lid inoculation with it since having to take the gloves off to feel the holes and mark them while I was in the middle of inoculating my jars would've been way too much of a hassle. would the fact I had to open the lid for about 5-10 seconds be enough to introduce contams? I also accidentally pushed down too hard on the syringe on that jar and ended up injecting a whole cc in just one spot. it would still colonize well, right?


--------------------
The meaning of life is just to be alive. It is so plain and so obvious and so simple. And yet, everybody rushes around in a great panic as if it were necessary to achieve something beyond themselves.
- Allan Watts


Looking for a P. Galindoi ATL#7 spore print/syringe. PM me to trade :pm:



Edited by MrSpadoodles (12/18/15 10:56 PM)


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InvisibleKalistis
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: MrSpadoodles]
    #22673960 - 12/18/15 09:51 PM (8 years, 1 month ago)

Dankington, I love your avatar/name combo. Makes me smile every time.


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InvisibleNumeroEno
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: Kalistis]
    #22674150 - 12/18/15 10:34 PM (8 years, 1 month ago)

Man I'm starting to get a little impatient waiting for these tubs to knot up. This is always the most exciting part. Got 3 more spawn bags in the PC with an hour to go. 2 of the bags are getting a pint of a cambo pin clone and one is getting a PE pin clone. I think starting with an invitro pin should cut down on potential blobbing. I didn't really give this clone a chance to sector, either. The cambo is from a culture where the whole plate gets covered in pins, so that will be fun too.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Offlinebuttermycoface


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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: NumeroEno]
    #22674598 - 12/19/15 01:42 AM (8 years, 1 month ago)



See any knots?


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OfflineEnergyTurtle
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: buttermycoface]
    #22675096 - 12/19/15 09:06 AM (8 years, 1 month ago)

I went with Doc_T's mini mono tub design, aka the "shotgun-mono hybrid" tub. The hardest part was scaling down the bulk sub recipe for a single 1/2 pint cake, lol, but I think I got the proportions about right.

I have a quick question though, maybe one of you guys can help me out? What's the point in covering my tub with a black bag during the colonization period? It seems unusual, when I was doing my BRF jars I was told not to worry about ambient light during colonization, and that the gas exchange from the jars should be unrestricted. Is the black bag more of a moisture barrier in this case, to keep my tub from drying out? Should I have wrapped the tub tightly so that no air can enter or escape? That didn't seem right to me, so I only covered the tub loosely, keeping the end of the bag loose so that the gases can enter and leave freely.


--------------------
"You decadent pig. Where the fuck do you get the nerve to go around telling those wops that I'm crazy? You worthless cocksucker. My Italian tour is already arranged for next spring & I'm going to do the whole goddamn trip wearing a bright red field marshal's uniform & accompanied by six speed-freak bodyguards bristling with Mace bombs & when I start talking about American writers & the name Tom Wolfe comes up, by god, you're going to wish you were born a fucking iguana!" - Hunter S. Thompson, 03/03/71 Letter to Tom Wolfe.


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Invisibledankington
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: Kalistis]
    #22675105 - 12/19/15 09:13 AM (8 years, 1 month ago)

Quote:

Kalistis said:
Dankington, I love your avatar/name combo. Makes me smile every time.




:laugh: Thanks, Kalistis! That made my day! I always thought either nobody ever got it or thought it was dumb. But now I'm feeling a bit peckish. I wonder if we have any marmalade.


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InvisibleInocuole
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: taGyo]
    #22675108 - 12/19/15 09:15 AM (8 years, 1 month ago)

Dunno how I missed this..

:standingby:


Gyo is that aa+ in the first post the print I gave you?  Kinda looks... totally different.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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