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InvisibleNumeroEno
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: taGyo]
    #22664693 - 12/16/15 07:15 PM (8 years, 1 month ago)

Cooking up enough millet for 3 G1 masters tonight. gonna do elm oyster and king oyster for sure, and maybe a reishi.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleKalistis
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: NDStepp84]
    #22664950 - 12/16/15 08:22 PM (8 years, 1 month ago)

Quote:

NDStepp84 said:

Normal pins coming in nicely, still more pins starting to form as well, so far looks like this tub will turn out decent.

I just started a grow log/grow along in my journal to log my experiments in detail and to shoot the shit with other members. Everyone here is welcome to stop by and join in. http://www.shroomery.org/forums/showflat.php/Number/22655204
I will continue to update here as well:peace:




Nice! I will look in on your progress.


--------------------


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OfflineMrSpadoodles
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: Kalistis]
    #22665727 - 12/16/15 11:35 PM (8 years, 1 month ago)

I only use the tape for the sterilization but not after that, correct?


--------------------
The meaning of life is just to be alive. It is so plain and so obvious and so simple. And yet, everybody rushes around in a great panic as if it were necessary to achieve something beyond themselves.
- Allan Watts


Looking for a P. Galindoi ATL#7 spore print/syringe. PM me to trade :pm:



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Offlinespacechildo
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: MrSpadoodles]
    #22665735 - 12/16/15 11:36 PM (8 years, 1 month ago)

you inoculate through the MP-tape and re-taping is optional. the dry verm barrier is your filter :thumbup:


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OfflineMrSpadoodles
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: spacechildo]
    #22665913 - 12/17/15 12:21 AM (8 years, 1 month ago)

alright, great. do any of you know any ways of raising the jars to not touch the water during sterilization? I've heard of people using jar lids, stones, and rags, but I don't have enough of any of those to do it. any ideas?
oh and how long do I have to wait for the jars to cool down to begin inoculation? I may or may not be able to inoculate friday but if I feel like I won't be able to I'd rather do it the same day of the sterilization.. if that's possible. it'd be like 3-10 hours later. will they be sufficiently cool by then?
and another thing: if I sterilize tomorrow as planned, it'll have been two days since I filled the jars. is this bad? I imagine that the sterilization would kill of any bacteria or spores that may have tried to grow in there during that time, but I rather get your opinion on that


--------------------
The meaning of life is just to be alive. It is so plain and so obvious and so simple. And yet, everybody rushes around in a great panic as if it were necessary to achieve something beyond themselves.
- Allan Watts


Looking for a P. Galindoi ATL#7 spore print/syringe. PM me to trade :pm:



Edited by MrSpadoodles (12/17/15 12:28 AM)


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Offlinespacechildo
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: MrSpadoodles]
    #22666178 - 12/17/15 01:50 AM (8 years, 1 month ago)

you can roll up some balls of alu foil, I did that for a long time with pf-cakes. rags scared me to death!
we are talking pf-tek cakes right? I cant see much problems leaving them prepped but not sterilized for a few days,
I know a great guy here who refridgerates his prepped grain before sterilizing them for a week or so.

once the 90mins are up you can take the lid of a normal pot, only PCs under pressure needs time to drop  pressure before opening the lid.
I'm sure the cakes will be cool after a few hrs on the counter.


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OfflinecronicrFacebook
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: spacechildo]
    #22666292 - 12/17/15 02:59 AM (8 years, 1 month ago)

Perception and response to gravity in higher fungi--a critical appraisal.
Abstract
Considering that research on gravitropism in higher fungi has a history of over 100 years, the harvest of established fact is disappointingly meagre. We can be reasonably certain of the following. Hymenomycete 'mushroom' fruit bodies (polypore and agaric) exhibit a number of tropisms of which anemotropism, gravitropism, phototropism and thigmotropism have been clearly demonstrated. At any one time one tropism usually predominates but the inferior tropisms can be demonstrated if the predominating ones can be removed by manipulation of the growth conditions. In ascending order, the hierarchy appears to be: thigmotropism, gravitropism, anemotropism, phototropism. During the course of development of a fruit body different tropisms predominate at different times. The youngest fruit body initials grow perpendicularly away from their substratum. The nature of this tropism is completely unknown but perpendicular growth of fruit body initials has been remarked upon in experiments at a variety of light intensities and in gravitational fields from +/- 0 to 4.5 g. The fruit-body primordium then becomes first positively phototropic but later negative gravitropism predominates. The switch between predominance of the two tropisms has been associated with the onset of sporulation in a number of different studies. The major adjustment of the direction of growth in response to a tropic stimulus is made by the mushroom stem. It is the apex of the stem which makes the most immediate gravitropic response. Gravitropic growth curvatures are limited to the normal growth zones of the stem and seem to depend on re-allocation of available growth resources. If the fruit body is reoriented late in the growth of the stem, it may not be able to respond fully. In these cases gravitropic movements of the cap may still be able to bring the hymenophore back to the vertical. Mechanical forces may influence and contribute to the 'gravitropic' response but this has not been experimentally examined. The hymenophore (gill, tube or tooth) is positively gravitropic and responds independently of the stem. Bracket polypores do not show tropisms but exhibit gravimorphogenetic responses such that gross disturbance leads to renewal of growth to produce and entirely new fruiting structure suitably reoriented to the new spatial position. One experiment performed on an orbiting space station suggests that, in the absence of a light stimulus, gravity may be required for initiation of fruiting in Polyporus brumalis. Otherwise, the indications from both clinostat and space-borne experiments are that the basic form of the mushroom (overall tissue arrangement of stem, cap, gills, hymenium, veil) in agaric and polypore alike is established independently of the gravity vector. Abnormal stem growth has been observed in clinostat cultures of Panus (= Lentinus) tigrinus and Polyporus brumalis, but the morphogenetic event which seems most dependent on gravity is sporulation (in the broadest sense). Cultures of P. brumalis on orbiting space craft fail to produce the poroid hymenophore and in clinostat experiments on the ground even karyogamy was rare in similar cultures. Coprinus cinereus grown on the clinostat was able to produce apparently normal fruit body primordia which failed to produce spores and then aborted, forming a new flush of primordia on the old. Taken together with the clear association between observation of gravitropism and the onset of sporulation, the implication is that commitment to the meiosis-sporulation pathway both requires the gravity vector and couples it in some way to fruit-body growth. There is no convincing evidence for a graviperception mechanism in fungi. There is no evidence for any organised means of communicating the gravitropic stimulus once it has been perceived. Reports of three different experimental studies reveal the authors' conviction that the apparently coordinated expression of gravitropic response is in truth a common, but independent, response by the individual component hyphae of the structure concerned. There is some evidence that in the negatively gravitropic Phycomyces sporangiophore the vacuole floats in the protoplasm. If this is generally true it could affect protoplasmic volumes above and below the vacuole such that a greater proportion of the cell's potential for wall growth was adjacent to the lower wall. This is not only an attractive way of accounting for asymmetric wall growth, but since the relative density of the vacuole can presumably be controlled by regulation of water influx and efflux, it is also an attractive means of accounting for the control of gravitropic responses. Phycomyces also exhibits a response to the mechanical consequences of reorientation which is additional to (and different from) the longer term gravitropic response. [TRUNCATED]
PMID: 11541309 [PubMed - indexed for MEDLINE]


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblestareatclouds
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: cronicr]
    #22666833 - 12/17/15 09:16 AM (8 years, 1 month ago)

Hmm.. yes... rather shallow and pedantic.


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OfflineEnergyTurtle
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: taGyo]
    #22666858 - 12/17/15 09:24 AM (8 years, 1 month ago)

Quote:

taGyo said:
Show me a pic Turtle, I have a jar of AA+ that's two months old at room temp that hasn't pinned :lol:





Haha, alright but go easy on me, this is my first time. I already know that this jar has a bacterial contamination, I thought that it would just die but the mycelium continued to grow and colonize anyway. If it's going to try to push out a few fruits, I'd like to nurture it and hopefully get some good prints from it.



Does that look like an aborted pin to you? The jar was knocked on 11/25, and this spot appeared just as the mycel were running out of substrate in the jar. It started out more of a tan, and then darkened to an olive-brown color as it pushed away from the mycel and up against the glass. It is visibly raised from the surface of the cake, like a pimple, but once it darkened up it stopped growing, so I'm guessing it aborted?

For reference, here's my only other jar that successfully inoculated. It was also knocked up on 11/25, and started showing a tiny spot of mycel growth under a single noc point about a week later. This is what it looks like now. Despite the extremely slow colonization speed, I feel much better about this jar. It just looks cleaner all around:



Also, I know that I got the wrong jar shape. They didn't have the shorties in 1/2 pint so I had to settle for the 1/2 pint tall boys.


--------------------
"You decadent pig. Where the fuck do you get the nerve to go around telling those wops that I'm crazy? You worthless cocksucker. My Italian tour is already arranged for next spring & I'm going to do the whole goddamn trip wearing a bright red field marshal's uniform & accompanied by six speed-freak bodyguards bristling with Mace bombs & when I start talking about American writers & the name Tom Wolfe comes up, by god, you're going to wish you were born a fucking iguana!" - Hunter S. Thompson, 03/03/71 Letter to Tom Wolfe.


Edited by EnergyTurtle (12/17/15 09:31 AM)


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Invisibledankington
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: EnergyTurtle]
    #22666876 - 12/17/15 09:30 AM (8 years, 1 month ago)

Your jar is fruiting to try and sporulate because of the contam. it's having trouble fighting.
The fruits always grow crazy when pressed up against the glass--but that doesn't look aborted. I say keep it away from other experiments and see what happens. Some have tried cleaning and spawning such jars with some success. But it appears you'll get something.

Maybe a TC around here can give better advice.


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InvisibleKalistis
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: EnergyTurtle]
    #22666883 - 12/17/15 09:33 AM (8 years, 1 month ago)

Hi energy. The top jar looks terribly wet and bacterial. :frown: Total bummer. If it looks completely colonized otherwise, crack it open and smell it. I'm betting it doesn't smell right.

I have only done bulk, so as far as the other jar, the mycelium looks better, but I'm not certain about the colonization time. How's your lid. Proper GE? Did you take off the foil? What are the temps like?


--------------------


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OfflineEnergyTurtle
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: Kalistis]
    #22666997 - 12/17/15 10:05 AM (8 years, 1 month ago)

Yeah it's a bummer for sure, I'll crack it and give it a whiff, but I really don't have high hopes. I was surprised to see it pinning at all, so maybe the shroom gods will smile and throw me a few fruits before it kicks the bucket. I'd like to see that, if only for the novelty of actually seeing a living cube in person, lol.

The GE seems pretty good, I removed the foil after sterilizing, and left the tape off after I inoculated. I let them colonize at room temperature on a shelf, but my room temperature is not ideal, it's about 68 degrees typically. Two days ago I started making a little 'nest' for that second jar by wrapping the bottom with a clean T-shirt, and that has boosted the colonization speed significantly, so I'm thinking the low temp might be a factor.

The lid itself is screwed on pretty tightly, would that be a problem considering that I have four uncovered holes and a dry verm barrier (as per pf tek and the "lets grow mushrooms" youtube vid)? I figured that tighter was better in this situation.

As for the bacterial contamination, I pretty much know where that came from: bad sterile technique, most likely during the spore syringe preparation. My dad insisted on 'teaching' me the techniques that he used in the 80's, and they're bad techniques lol. I told him several times, but he insisted on doing it his way for the first try. So that's whatever, no skin off of my bones, just wasted time lol. I've already given him a guilt trip about it so hopefully he'll let ME teach HIM next time. :P


Edited by EnergyTurtle (12/17/15 10:42 AM)


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OfflinetaGyo
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: EnergyTurtle]
    #22667171 - 12/17/15 10:45 AM (8 years, 1 month ago)

That jar doesn't look good to me as well,

You got great advice, I'd toss that. Whenever my myc comes out looking like that it's always bacteria. The extent of damage determines what I do with it.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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InvisibleNumeroEno
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: taGyo] * 1
    #22667339 - 12/17/15 11:27 AM (8 years, 1 month ago)

I love when you put a tub into fruiting. I put this one and the other APE tub into fruiting last night, and opened this one just now to admire it.



It's just lovely. It smells so earthy and alive and the rhizos poking through the casing look like snowflakes.

Once they start knotting and pinning you can feel this crazy energy coming from the substrate. or maybe I'm just crazy :crazy2:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineNDStepp84
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: NumeroEno]
    #22667430 - 12/17/15 11:53 AM (8 years, 1 month ago)

Looks great Eno! I love all the smells as well, I miss the smell of mixing up brf cakes. I plan to start a few cakes soon with a clone that's been in the fridge for about 6 months. I have an idea, more of a novelty grow really.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflinetaGyo
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: NumeroEno]
    #22667611 - 12/17/15 12:48 PM (8 years, 1 month ago)

Quote:

NumeroEno said:
I love when you put a tub into fruiting. I put this one and the other APE tub into fruiting last night, and opened this one just now to admire it.



It's just lovely. It smells so earthy and alive and the rhizos poking through the casing look like snowflakes.

Once they start knotting and pinning you can feel this crazy energy coming from the substrate. or maybe I'm just crazy :crazy2:



:solidnod:

I love the smells too, I love the smell of freshly bucket-tekked coir.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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InvisibleNumeroEno
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: taGyo]
    #22667616 - 12/17/15 12:50 PM (8 years, 1 month ago)

Speaking of coir, the untreated coir is killing it as a casing :yesnod:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineMrSpadoodles
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: NumeroEno]
    #22669176 - 12/17/15 07:24 PM (8 years, 1 month ago)

:Feelstrippyman:


--------------------
The meaning of life is just to be alive. It is so plain and so obvious and so simple. And yet, everybody rushes around in a great panic as if it were necessary to achieve something beyond themselves.
- Allan Watts


Looking for a P. Galindoi ATL#7 spore print/syringe. PM me to trade :pm:



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OfflineEnergyTurtle
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: NumeroEno]
    #22671179 - 12/18/15 09:22 AM (8 years, 1 month ago)

Quote:

NumeroEno said:
I love when you put a tub into fruiting. I put this one and the other APE tub into fruiting last night, and opened this one just now to admire it.



It's just lovely. It smells so earthy and alive and the rhizos poking through the casing look like snowflakes.

Once they start knotting and pinning you can feel this crazy energy coming from the substrate. or maybe I'm just crazy :crazy2:




That's a beautiful tub, I'm getting hungry just thinking about it. :P

I cracked my bacterial jar this morning and gave it a good whiff, but I didn't catch any noticeable odors. A faint hint of fresh mushrooms, but thankfully nothing else. Maybe this jar will be good to me. Like it knows that I fucked up, but it's gonna perform just well enough to really hook me into this hobby and give me a craving for better results, lol. Is that anthropomorphism? :P I like to believe that the mushrooms have an intelligence of their own.


--------------------
"You decadent pig. Where the fuck do you get the nerve to go around telling those wops that I'm crazy? You worthless cocksucker. My Italian tour is already arranged for next spring & I'm going to do the whole goddamn trip wearing a bright red field marshal's uniform & accompanied by six speed-freak bodyguards bristling with Mace bombs & when I start talking about American writers & the name Tom Wolfe comes up, by god, you're going to wish you were born a fucking iguana!" - Hunter S. Thompson, 03/03/71 Letter to Tom Wolfe.


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OfflinetaGyo
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Re: Gyo's Better Grows (Community Grow-A-Long) [Re: EnergyTurtle]
    #22671188 - 12/18/15 09:24 AM (8 years, 1 month ago)

I would spawn it,

I'd say 85% of grows have a level of bacteria influencing their results. I actually think bacterial spawn colonizes faster


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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