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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Mixing two complementary isolates?
#22503480 - 11/10/15 03:40 AM (8 years, 2 months ago) |
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Say you have one large fruit prolific yielding strain, and another overpinning small fruit isolate that yields poorly with extreme potency.
Would mixing say, 2qt of spawns of each variety into a tub produce fruits that were some type of multigenetic phenotypic average of the two isolates?
Would mixing the isolates, either by double wedging/doubleg2ging a jar, or 50/50 mixing colonized grain, leaving them to exchange genetic material in the jar, then spawning them and fruiting them have the potential to give a more normalized version of the two? I would then try to clone and sector the best fruits.
I'm wondering about this cause I got some amazing potency on a prolifically pinning isolate that produces such small fruits the yield is shit. Don't have a rattlesnake around to milk. It seems like trying to get them to exchange genetic information in a jar would provide way better contact surface area than trying to create contact on an agar plate, and create more diversity amongst the shared genetic strains?
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
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It doesnt really work like that.
If you wanna see just put a wedge from each plate on a blank plate
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 Amateur Mycologists United AMU Q&A
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: Mixing two complementary isolates? [Re: mushpunx]
#22503659 - 11/10/15 05:34 AM (8 years, 2 months ago) |
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Well:
Fruits can contain more than one strain, I know this from cloning.
And supposedly strains living near eachother exchange genetic information over time.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Put a wedge of both across from each other on a plate. Hope that they form a 3rd sector via amasamatosis, if you do get lucky test it. If no luck back to step one.
Or inoculate a jar with both hope some mixing occurs clone fruits with the best of both if lucky
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
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Re: Mixing two complementary isolates? [Re: bodhisatta]
#22504044 - 11/10/15 08:31 AM (8 years, 2 months ago) |
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (11/10/15 08:33 AM)
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Mixing two complementary isolates? [Re: bodhisatta]
#22504396 - 11/10/15 10:19 AM (8 years, 2 months ago) |
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Quote:
bodhisatta said: Put a wedge of both across from each other on a plate. Hope that they form a 3rd sector via amasamatosis, if you do get lucky test it. If no luck back to step one.
Or inoculate a jar with both hope some mixing occurs clone fruits with the best of both if lucky
this is an interesting idea.
I'd been curious if strain conglomerates could outperform their individually isolated constituents. Would be a difficult experiment to perform well though.
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