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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 9 hours
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Bacillus, a question
#22498283 - 11/08/15 10:09 PM (8 years, 2 months ago) |
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Hi, I just made my first agar petris, and decided to wait two days before inoculating them, to see if there's any contam present.
now all my plates have this shitty bacillus on them. I made them like this :
- boiled some potato for 15 mins in a pot
- dissolved 9 grams of agar in the hot water from boil
- poured the petris
- put them in microwave oven, and let them receive that heat for ~3 minutes, they stated to boil like hell in petris.
- let them cool down in the microwave oven, didn't even touch them.
- now after two days, they show contam signs
I know it was not a good idea to do no-pour teks in glass petris. but was just afraid of pour teks.
So my question is, is bacillus bacteria resistant to microwave waves ? (and the heat from boiling) Can someone direct me to some info about heat-resistance of microbes ? (like temps they die at?)
Edit, some pics of petris :

will read your answers in 4 hrs, have to hit my job for now. Thanks.
Edited by psilocybist (11/08/15 10:19 PM)
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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they may have survived the microwave or may have gotten sucked in on cool down or both.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Bacillus, a question [Re: drake89]
#22501465 - 11/09/15 05:13 PM (8 years, 2 months ago) |
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I would pressure cook it and sterilize it.
The 15 minutes or so at 120C should be enough to kill endospores.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Bacillus, a question [Re: micro] 1
#22504070 - 11/10/15 08:41 AM (8 years, 2 months ago) |
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Microwaves have no place in mycology.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 9 hours
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Re: Bacillus, a question [Re: 36fuckin5]
#22504870 - 11/10/15 12:25 PM (8 years, 2 months ago) |
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Thanks all for replies. just found out these damn bacillus bacteria are thermophilic too. so my sterilization was not good enough.
will try again.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Quote:
psilocybist said: just found out these damn bacillus bacteria are thermophilic too. so my sterilization was not good enough.
You identified the actual species? I don't think there is much of a way to do that without PCR and gels.
Bacillus spp. are very common and broad; it will contain thermophiles but I'd be surprised if it were that common (though looking it up it seems Bacillus stearothermophilus is a common cause of food spoilage). You'd expect thermophiles to be near hot springs or an ocean spreading ridge or something.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 3 months, 19 days
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Quote:
psilocybist said: put them in microwave oven,

A microwave is no good for agar, or any sterilization. The best thing you could use would be a pressure cooker like everyone else. A microwave might be useful for heating your water so it dissolves the agar before pressure cooking but it is not a necessary step.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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