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Offlineuninc4life2010
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Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar?
    #22497391 - 11/08/15 06:59 PM (8 years, 2 months ago)

I inoculated my first ever WBS quart jars with agar from a PE clone I took back a few months ago.  I managed to get 7 jars out of one plate, which I think is pretty good.  Do I need to flame sterilize between each jar inoculation, or is it nly necessary to do so at the very beginning before I cut the first wegde?


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OfflinePsilosoulful

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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: uninc4life2010]
    #22497469 - 11/08/15 07:13 PM (8 years, 2 months ago)

I would flame sterilize after each time you cut a wedge out. So yeah, after every jar.


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OfflineMachiavelliavore
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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: uninc4life2010]
    #22497476 - 11/08/15 07:14 PM (8 years, 2 months ago)

I would cut all 7 wedges at once, then reflame if you do something stupid while stabbing/dropping the wedges.  I guess if you're scared of hidden bacteria it would be better to flame cut flame cut...


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflinePsilosoulful

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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: Machiavelliavore]
    #22497485 - 11/08/15 07:16 PM (8 years, 2 months ago)

:awesomenod:


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OfflineRogerRabbitM
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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: uninc4life2010]
    #22497553 - 11/08/15 07:33 PM (8 years, 2 months ago)

Flame at the start of each petri dish, but if nothing unholy touches the scalpel tip, you don't need to reflame between jars.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlineuninc4life2010
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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: RogerRabbit]
    #22497560 - 11/08/15 07:34 PM (8 years, 2 months ago)

Thank you!


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