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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar?
#22497391 - 11/08/15 06:59 PM (8 years, 2 months ago) |
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I inoculated my first ever WBS quart jars with agar from a PE clone I took back a few months ago. I managed to get 7 jars out of one plate, which I think is pretty good. Do I need to flame sterilize between each jar inoculation, or is it nly necessary to do so at the very beginning before I cut the first wegde?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: uninc4life2010]
#22497469 - 11/08/15 07:13 PM (8 years, 2 months ago) |
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I would flame sterilize after each time you cut a wedge out. So yeah, after every jar.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: uninc4life2010]
#22497476 - 11/08/15 07:14 PM (8 years, 2 months ago) |
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I would cut all 7 wedges at once, then reflame if you do something stupid while stabbing/dropping the wedges. I guess if you're scared of hidden bacteria it would be better to flame cut flame cut...
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: Machiavelliavore]
#22497485 - 11/08/15 07:16 PM (8 years, 2 months ago) |
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: uninc4life2010]
#22497553 - 11/08/15 07:33 PM (8 years, 2 months ago) |
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Flame at the start of each petri dish, but if nothing unholy touches the scalpel tip, you don't need to reflame between jars. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Re: Is it necessary to flame sterilize my scalpel between each jar inoculation when using agar? [Re: RogerRabbit]
#22497560 - 11/08/15 07:34 PM (8 years, 2 months ago) |
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Thank you!
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