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Invisiblenatzyshroomer
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Registered: 12/01/12
Posts: 405
PDA vs Nutrient agar
    #22497085 - 11/08/15 06:02 PM (8 years, 2 months ago)

I have the option to purchase agar solution from a store I have been thinking and from my reading it seems like the Potato dextrose agar would seem like a better fit but i may even end up trying both out of curiousity

PDA
This dehydrated culture medium is ideal for cultivation and isolation of yeasts and molds from dairy products and other food products.
Potato Dextrose Agar contains...
    Infusion from Potatoes 10%
    Dextrose 51%
    Agar 39%
Nutrient Agar
This dehydrated culture medium is used for the cultivation of a wide assortment of microorganisms as well as a base to which a variety of materials are added to create selective, differential, or enriched media for specific organisms.

Nutrient Agar contains:

    Beef Extract 13%
    Peptone 22%
    Agar 65%


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: PDA vs Nutrient agar [Re: natzyshroomer]
    #22497186 - 11/08/15 06:18 PM (8 years, 2 months ago)

Nutrient agar is better for bacteria. Use PDA for fungi.


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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: PDA vs Nutrient agar [Re: natzyshroomer]
    #22497201 - 11/08/15 06:20 PM (8 years, 2 months ago)

if i make a sleeve of PDYA with flakes, its
10g agar
10g flakes
7g dextrose
1g yeast

dextrose = ~25%
agar = ~35.7%
potato = ~35.7%
yeast = ~3.6%


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineRo0k
Stranger

Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
Re: PDA vs Nutrient agar [Re: blindingleaf]
    #22497290 - 11/08/15 06:38 PM (8 years, 2 months ago)

Either will work fine for your purposes. I have used both for agar work and you get growth on them. For me, I have like 100g of nutrient agar so when I've used up the agar powder I have left i'll switch to that. It comes down to ease od access and price. Nutrient agar cost me $40 for a tub and while the mats for pda might be cheaper i end up having to run around for an hour or so to get it.


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