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Offlinemushroomnoob1981
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Too long/too hot sterilization time?
    #22495695 - 11/08/15 01:29 PM (8 years, 2 months ago)

So I had 10 pint and a half jars filled with rye berries prepared with the RR method.  My PC is somewhat small (holds 4 of these at a time) and only goes to 12 PSI (I've read this is OK but I let the thing go for 2.5 hrs to try and make up for the slightly lower pressure). 


To try and save some time, I throw them in an autoclave I have access to.  For some reason, I decided to sterilize at 130°C (this equates to 24 PSI) for 60 min.

After I take them out of the autoclave, I notice that the rye berries have darkened and seemed less "bulgy" (i.e. they shrunk a little).  For the record, I have accidentally completely crisped rye berries this time I didn't put enough water in my PC, all of the water evaporated & the PC essentially turned into an oven.  Those things looked dark and brittle.  The current batch does not look like that, just a bit darker and smaller than they were pre-sterilization. 

Do you think I drove off too much moisture and made the rye berries unusable?

EDIT- Also, I'm not sure if this is of any consequence, but the lids were capped with 100% Tyvek (i.e. I discarded the cap part of the lid, placed a piece of Tyvek over the mouth and screwed the ring over the Tyvek).  The jars were also covered with aluminium foil during this process. 


Edited by mushroomnoob1981 (11/08/15 01:29 PM)


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Offlinenewrook
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Re: Too long/too hot sterilization time? [Re: mushroomnoob1981]
    #22495728 - 11/08/15 01:36 PM (8 years, 2 months ago)

Are autoclaves dry heat?


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Offlinemushroomnoob1981
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Re: Too long/too hot sterilization time? [Re: newrook]
    #22495811 - 11/08/15 01:53 PM (8 years, 2 months ago)

The autoclave uses compressed steam.  It works just like a PC except:

A-It can reach higher temps/pressures than most PC's (134°C/30 PSI vs 121°C/15 PSI).

B-It cools more rapidly than a PC because (at least this one) cools by slowly venting the steam through an exhaust port (I'm pretty sure this doesn't cause rapid evaporation/drying because people use it for liquid media all the time).

There is an option to dry your samples with dry heat after sterilization, but I'm 100% sure that feature wasn't turned on.


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