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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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I HATE SAB'S!
#22495373 - 11/08/15 11:59 AM (8 years, 2 months ago) |
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Whenever I use one, i work meticulous to keep everything as clean as possible. Half the time the projects, whether it be G2G or agar work, end up all over the bottom as I have never had to do anything more cumbersome in my life. The other half of time when the projects don't end up all over the place, my hard work and time results in contaminates anyway. I think I'm going back to old school syringe to grain jars until I can afford a flow-hood. Just a rant
My latest failure haha:
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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If you think a flow hood is gonna be easy you might be in for a nasty surprise. If anything a flow hood is tricky because you need to have just as tight of procedure, if not better. I don't have a hood, I would like one for sure, but I am quite sure that years of toiling in a SAB will serve me well by the time I get one.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Pasty plates are nice in a SAB cause they don't spill 
I feel your pain, i placed my arm holes too close together so now i feel like im working with handcuffs on 
Even doing pf-tek i have spilt two jars all over the bottom!
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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Very true. I think my best bet would be to master the SAB, but I need to toss this crappy one and make one way bigger so Its at least somewhat manageable!
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Do a couple dry runs to get your technique down..that way you know exactly what and how you are going to do it...otherwise you are just freestyle SAB working, and all sorts of unforseen crap happens.
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Q&A US vs. THEM The more I learn, the less I know.
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ShroomerInTheRye
Clit Commander



Registered: 01/12/12
Posts: 13,036
Loc: Themyscira
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I'm curious...are you working in a room with a vent for the AC?
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<-- Clicky Clicky
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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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Yea but i make sure to turn it off at least an hour prior or so. My problem most likely stems from having 6 pets though I wouldn't wanna know whats floating around in this house
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DaveyJones6911
Nonconformist


Registered: 09/08/15
Posts: 690
Loc: EU
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there's an easier way to work with agar: http://www.shroomery.org/forums/showflat.php/Number/19208976
i tried petrie dishes because someone gave me a bunch of them a long time ago, but i too found them difficult to handle. the tek in the link offers an option where the agar has solidified before it gets to the SAB.
i have found that to be very helpful.
-------------------- Creativity is a dangerous thing in the eyes of those who don't have any.
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StickyIcky Fingers
Free DOOM!! Available Here

Registered: 12/03/13
Posts: 178
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When I started using one practicing what I was going to be doing helped a lot. I would put everything in and go through the motions without opening anything before spraying the SAB and actually doing the clean work. It helped me see what the hard parts were going to be instead of trying to figure out what order to do things in and trying to maintain decent sterile technique. It helped me to get my tools and jars lined up and in an accessible part of the SAB so I could reach everything I needed to use with whatever hand was going to need it.
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TheScientificMethod
Psychonautic Explorer & Writer



Registered: 02/20/14
Posts: 632
Loc: USA
Last seen: 21 days, 20 hours
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I have to agree with you OP. I used to use SABs and they were more of a pain in the ass than anything else. In fact I almost lit my house on fire once because of an SAB. I was trying to be a sterile as possible and so I had a rag soaked in alcohol in my SAB and I flamed a needle, only to set the rag, then the whole box on fire (Stupid, I know, but I've learned my lesson). The entire box turned into a ball of fire and my hands were stuck inside, so I frantically was trying to get my hands free, flung the box on the carpet and almost set the carpet on fire... that was a scary moment.
Anyways, since that day I have forgone the SAB and created a Still Air LAB. I literally set up my old MMJ grow tent (4x6x8'). I clean the hell out of the inside the night before then lysol the entire thing. I make it as clean as possible, then I pressure cook/sterilize a tyvec suit and mask. So when I go in there I'm as clean as possible. That way I can work without restriction to my movement. I've found this to work a lot better, but I still do deal with contamination from time to time.
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ShroomerInTheRye
Clit Commander



Registered: 01/12/12
Posts: 13,036
Loc: Themyscira
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I live in a tiny little apartment with 2 large cats. I only have a 6' x 2' space to work in. I hung a white shirt over the vent and closed the shirt holes with a hair clip. Honestly, it was disgusting how fast that shirt turned yellow, and I don't smoke inside. Smelled awful too. I sent some prints off a few weeks ago taken in my SAB, and they turned up with no contams.
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<-- Clicky Clicky
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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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Wow that sounds traumatizing! glad your alright! Love the idea of a SAL btw, the SAB just isn't big enough for efficient work. I've never had a part of the hobby i've disliked as much as working in these things
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Get a bigger sab op
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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I have to try this now, scared what the results might be though. So much dust, and I smoke inside aha
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candela
Stranger


Registered: 09/04/15
Posts: 281
Last seen: 2 years, 6 months
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Dude, I am totally new to this hobby (2 months) just like you. I have already knocked up over 100 jars without any contams yet, by just using an SAB.
What I do: I literally spray the inside of my SAB with Alcohol 70% till its dripping on the walls (Dont wipe it dry!). Leave the inside of the SAB soaked, and dripping with alcohol while your doing your transfers and inoculations.
I maybe new to this and I don't know if this may be some good advice to give to you, but it has worked well for me so far.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: I HATE SAB'S! [Re: candela]
#22495581 - 11/08/15 12:58 PM (8 years, 2 months ago) |
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Be careful with that much iso and a flame source/hot tools. It can lead to 
Bleach or soapy water really do work good for a SAB and you are much more fire marshal friendly
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Arush
Godfather



Registered: 05/06/13
Posts: 312
Loc: Canada
Last seen: 5 years, 9 months
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Personally I think spore syringes are easier. It may take 5 days longer for the spores to start growing but much less chance of contams. Cap>print>syringe>inoculate. Also you don't have to use petri dishes.. half pint jars would as well and won't over flow.
-------------------- http://www.shroomery.org/forums/showflat.php/Number/22409475 “Psychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behaviour and information processing. They open you up to the possibility that everything you know is wrong.” ― Terence McKenna
Edited by Arush (11/08/15 01:02 PM)
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candela
Stranger


Registered: 09/04/15
Posts: 281
Last seen: 2 years, 6 months
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Quote:
Toadstool5 said: Be careful with that much iso and a flame source/hot tools. It can lead to 
Bleach or soapy water really do work good for a SAB and you are much more fire marshal friendly 
Yes. Place your flame source (alcohol lamp) far away, but still within arms length from your SAB.
Edited by candela (11/08/15 01:04 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: I HATE SAB'S! [Re: Arush]
#22495593 - 11/08/15 01:03 PM (8 years, 2 months ago) |
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Quote:
Arush said: Personally I think spore syringes are easier. It may take 5 days longer for the spores to start growing but much less chance of contams. Cap>print>syringe>inoculate.
Unless of course the spore syringe is full of contams. Which happens a lot. In fact it's pretty much impossible to guarantee a syringe won't have any contams at all.
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Arush
Godfather



Registered: 05/06/13
Posts: 312
Loc: Canada
Last seen: 5 years, 9 months
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I know, still less of a chance imo. Plus you can make your syringes very dark giving you way more innoc points.
-------------------- http://www.shroomery.org/forums/showflat.php/Number/22409475 “Psychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behaviour and information processing. They open you up to the possibility that everything you know is wrong.” ― Terence McKenna
Edited by Arush (11/08/15 01:04 PM)
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TriggerFish
Stranger

Registered: 09/23/15
Posts: 25
Last seen: 8 years, 2 months
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Quote:
donkeycong said:
My latest failure haha:

Well, I know one thing that could help you. Having petri dishes sit on that uneven plastic on the bottom can definitely result in catastrophe as its not the most stable surface, especially when your arms are moving the box around.
Turn the box over, don't use the lid. So even if your box moves around a little, the items you have in the box are still on a non moving surface such as the table you are working on. Vision through the bottom of the box could be an issue, so I cut a huge rectangle out of the bottom and hot glued a nice piece of thick transparent plastic in place of it. I also added a line of hot glue all around the top edge (bottom when u are working) of the box, so it sorta holds to the table when I'm working.
Capisce?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: I HATE SAB'S! [Re: Arush]
#22495605 - 11/08/15 01:06 PM (8 years, 2 months ago) |
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Quote:
Arush said: I know, still less of a chance imo. Plus you can make your syringes very dark giving you way more innoc points.
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Arush
Godfather



Registered: 05/06/13
Posts: 312
Loc: Canada
Last seen: 5 years, 9 months
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-------------------- http://www.shroomery.org/forums/showflat.php/Number/22409475 “Psychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behaviour and information processing. They open you up to the possibility that everything you know is wrong.” ― Terence McKenna
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,359
Loc: The Inexpressible...
Last seen: 47 minutes, 55 seconds
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I would use different gloves, ones with long cuffs. Your skin could be getting into the air, and skin has lots of microbes growing on it.
Also, your walls are dry. They should be coated with soapy or bleachy water.
As for a hard surface. I would recommend buying a smaall piece of thick, heavy plywood, wrap it something non porus like a thick garbage bag, taping it tight, and put that inside thee bottom of the SAB. Make sure it fits inside the bottom of the SAB in the best fit as possible.
All these should help u out.
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
  Oregon Eclipse Festival 2017 :: Aug 19th - 21st :: Pure Paradise   Very Effective LSA Extraction Tek | 💧 Advanced Cold Water LSA Extraction Method 💧 |  Mescajuana - Mescaline with Marijuana | DMT Dab Bongs | UFO Technology! Shpongle
     
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: I HATE SAB'S! [Re: Arush]
#22496277 - 11/08/15 03:34 PM (8 years, 2 months ago) |
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Dont tell me bout contam issues dude. I really dont wanna hear the word agar right now. This is this weeks work all for the trash. Except for the jar. That one is older

Some of the worst contaminated plates

This week was a nightmare. I have never been so careful yet I have never experienced this much contams.
At first I thought this is happening because of a faulty sleeve

But then I made a new batch after cleaning the shit out of my SAB with bleach and soap and im still getting this fucking yellow mold. Its really starting to wear me down. For one I cannot afford to keep buying new shit every few days. And on the other hand you start to lose interest in something when all you get are losses. Worst part is I don't know wtf is this yellow piece of shit mycelium and even in contam forum they couldn't name the bitch.
Today ill go make one last buy at the supermarket for a bigger SAB but if I dont get a win soon I will throw all the agar related shit in the can. The funny thing is( makes me wanna cry tho) I knock up my jars with LC in open air and do agar in SAB. I never failed this hard with grains which make it so much worse.
I feel you man atm even looking at my SAB makes me mad as hell
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Try no pour. Cheaper and easier than pour IMO.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Dont get those kinda bowls here. The ones we do get cost as much as a sleeve per bowl. Not really worth it.
Edit: meh if I lived in the US I wouldn't have spent half of what I already did to get this far. And I really didn't get far as of yet
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Use jars. I assume you have jars. Keep in mind a methods worth is dependent on if it works or not.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Got pint jars. Only 7 wide mouth though that are short enough. Rest are ball which are kinda tall to work with.
I dunno If I can't get it to work with what I have I am definitely stopping there as far as agar goes. Sucks but anymore of this and ill go crazy.
Do you know what kinda mold it is by the way? That's what driving me nuts most.
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Plague_Wraith
Horseman


Registered: 07/22/15
Posts: 79
Last seen: 8 months, 20 days
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I hate using a SAB as well. I don't know that I can accurately convey my rage.
I went out of my way to purchase a 116 quart Sterilite tote and staggered the arm holes using a hole saw set for comfort. It is still an unbelievably clunky process to use it. Even the process of filling a syringe with LC inside of there was enough to make me want to throw it off the deck. I cut 4" holes and my arms ended up getting stuck as well. Talk about pissing me off. I tried knocking up grain jars with a 60cc syringe with a 4 inch, 14 gauge dispensing needle in there too. Screw trying to do that for 11 jars. I ended up doing half of them in open air. Next time I'm going totally open air. I'll recut the holes at 5" and try it again for agar in a week. So far I've had zero contams in open air.
Stupid SAB.
-------------------- Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
Pastywhyte said: Try no pour. Cheaper and easier than pour IMO.
i just poured no pours like petri's last night
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: I HATE SAB'S! [Re: cronicr]
#22496547 - 11/08/15 04:32 PM (8 years, 2 months ago) |
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Quote:
cronicr said:
Quote:
Pastywhyte said: Try no pour. Cheaper and easier than pour IMO.
i just poured no pours like petri's last night
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Knocking up jars in open air is not so bad if you do it right. I tilt the jar slightly and peel back the tyvek just enough to see the hole. The tilt and position of the jar makes sure that the tyvek still covers the hole from above. Then I insert the syringe and cover that with the tyvek while I shoot LC in it real fast. Dont have many contam issues this way.
Dang you have trouble sucking up LC with a 116quart SAB? Thats one of the reasons I was going to risk buying it. If agar doesn't work out at least I can do my grains and LC's in there. I am using a bigger smaller syringe though so I guess we'll see.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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DrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
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You could try lowering your PH to around 5.6 so your medium is more selective for myc instead of bacteria.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: I HATE SAB'S! [Re: cronicr]
#22496579 - 11/08/15 04:37 PM (8 years, 2 months ago) |
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Quote:
cronicr said:

Nice. Might try that next time I need to hot pour.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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was a total accident lol, was just going to run them through the pc because they were all contaminated plates and i i figured i would clean them before putting them away and was running the pc for agar anyway....pulled the agar out and 3 jars with 10 salad cups in each and thought"why not just pour these fuckers" lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: I HATE SAB'S! [Re: cronicr]
#22496606 - 11/08/15 04:42 PM (8 years, 2 months ago) |
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Lol sounds like you
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Plague_Wraith
Horseman


Registered: 07/22/15
Posts: 79
Last seen: 8 months, 20 days
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Quote:
Supalemonhaze said: Dang you have trouble sucking up LC with a 116quart SAB? Thats one of the reasons I was going to risk buying it. If agar doesn't work out at least I can do my grains and LC's in there. I am using a bigger smaller syringe though so I guess we'll see.
I know, right. Sounds like I'm handicapped. In truth though, I was using a half-gallon worth of LC and that particular LC jar has Polyfill stuffed in the top for use as a GE filter. That means I didn't want the polyfill getting wet at all for fear of contams getting wicked in.
I was trying to hold the jar at an angle so my 4" needle would reach the large cluster of mycellium on the bottom (didn't use a stir bar, just marbles). It was a hell of a process to get the syringe totally full. I had to actually lay the entire LC jar sideways to pull it off. Thankfully the polyfill is stuffed in there so tight nothing leaked out.
I'm sure the whole process would have been much easier if I wasn't using such massive tools (half-gallon jar, 60cc syringe, 4" dispensing needle) and if I had a proper LC filter and stir bar, but I was making do.
-------------------- Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
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Quote:
Plague_Wraith said: I hate using a SAB as well. I don't know that I can accurately convey my rage.
I went out of my way to purchase a 116 quart Sterilite tote and staggered the arm holes using a hole saw set for comfort. It is still an unbelievably clunky process to use it. Even the process of filling a syringe with LC inside of there was enough to make me want to throw it off the deck. I cut 4" holes and my arms ended up getting stuck as well. Talk about pissing me off. I tried knocking up grain jars with a 60cc syringe with a 4 inch, 14 gauge dispensing needle in there too. Screw trying to do that for 11 jars. I ended up doing half of them in open air. Next time I'm going totally open air. I'll recut the holes at 5" and try it again for agar in a week. So far I've had zero contams in open air.
Stupid SAB. 
I feel the same way, I used a sab for about 2 years but in the past year? I used it once, and that may be my last time. I hate doing sab work with a passion, I don't know why I just feel very restricted and clumsy in them. I had a large tote, armholes spaced apart nicely, still hated it.
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ShroomerInTheRye
Clit Commander



Registered: 01/12/12
Posts: 13,036
Loc: Themyscira
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Re: I HATE SAB'S! [Re: invitro]
#22496808 - 11/08/15 05:16 PM (8 years, 2 months ago) |
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To the folks who don't use a SAB, do y'all make prints?
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<-- Clicky Clicky
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
Plague_Wraith said:
Quote:
Supalemonhaze said: Dang you have trouble sucking up LC with a 116quart SAB? Thats one of the reasons I was going to risk buying it. If agar doesn't work out at least I can do my grains and LC's in there. I am using a bigger smaller syringe though so I guess we'll see.
I know, right. Sounds like I'm handicapped. In truth though, I was using a half-gallon worth of LC and that particular LC jar has Polyfill stuffed in the top for use as a GE filter. That means I didn't want the polyfill getting wet at all for fear of contams getting wicked in.
I was trying to hold the jar at an angle so my 4" needle would reach the large cluster of mycellium on the bottom (didn't use a stir bar, just marbles). It was a hell of a process to get the syringe totally full. I had to actually lay the entire LC jar sideways to pull it off. Thankfully the polyfill is stuffed in there so tight nothing leaked out.
I'm sure the whole process would have been much easier if I wasn't using such massive tools (half-gallon jar, 60cc syringe, 4" dispensing needle) and if I had a proper LC filter and stir bar, but I was making do. 
My problem with LC in agar was also a big jar ( I think a little bigger than a quart) but mostly it was cos my SAB is only 50litres. Thats why I ended up doing eveything open air. Was still using micropore as a filter lmao. I think I didn't experience disaster with it because I only made it from 200ml of honey water since it was my first lc and I didn't know better. Hopefully ill have better luck working with pints and a bigger SAB. I really hope agar will work out in the end too. I think its most rewarding and fun part of mycology and a shame not to be able to do it.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
ShroomerInTheRye said: To the folks who don't use a SAB, do y'all make prints?
I did one PE print with water tek so far. Didn't use a SAB for it so im not sure if ill be able to use it if agar doesn't work out.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
ShroomerInTheRye said: To the folks who don't use a SAB, do y'all make prints?
I'd be more concerned with how you gonna play with agar! when I just take a cpl prints I just sanitize a small ice cream box and flip it over a towel. prints inside, under shotters if its pans/tiny caps species.
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Plague_Wraith
Horseman


Registered: 07/22/15
Posts: 79
Last seen: 8 months, 20 days
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Quote:
Supalemonhaze said:
My problem with LC in agar was also a big jar ( I think a little bigger than a quart) but mostly it was cos my SAB is only 50litres. Thats why I ended up doing eveything open air.
I really hope agar will work out in the end too. I think its most rewarding and fun part of mycology and a shame not to be able to do it.
A 50 liter SAB? Jesus, I probably would have snapped my own neck trying to fill my LC syringe in that amount of space. It'd definitely be worth an upgrade. Stealth is pretty much out the window with something around the 116 quart size though and the price is enough to make you think twice.
I've been very interested in agar lately. I can't wait to try it. I agree, it's gotta be rewarding to clone some massive shrooms or to even save a dirty culture.
-------------------- Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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You should see the prices for totes in my country then :p. 50l=25euro.
Its no wonder starting mycology is the most expensive hobby I ever started.
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Ive been using a 58qt SAB forever and last night I cut arm holes in a 110qt tub and its friggen great I feel like a dummy for not doing it sooner ive been doing jars 5 at a time without the proper headroom for g2g transfers now I can prolly do 20 quarts at once!! And without letting my sab settle 3-5 times per session from reloading jars/supplies big ass SAB FTW
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Hopefully ill share your luck bout 5 more hours till the supermarket opens.
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Plague_Wraith
Horseman


Registered: 07/22/15
Posts: 79
Last seen: 8 months, 20 days
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Quote:
Supalemonhaze said: Its no wonder starting mycology is the most expensive hobby I ever started.
Yeah, no joke. It took me 3 years of working a miserable job to save up enough to buy the supplies I've recently acquired. Everytime I put together a list of things I need for this hobby I feel like I'm buying weird stuff too. "Yeah, gotta buy another strainer!" "Gotta buy a scalpel and a big box of garbage bags!" "Need a hole saw to drill up a tote!" I look at my SAB and wonder what people outside of our hobby would think of it. They'd probably be wondering what the hell was going on. 
Quote:
tetherface said: Ive been using a 58qt SAB forever and last night I cut arm holes in a 110qt tub and its friggen great I feel like a dummy for not doing it sooner ive been doing jars 5 at a time without the proper headroom for g2g transfers now I can prolly do 20 quarts at once!! And without letting my sab settle 3-5 times per session from reloading jars/supplies big ass SAB FTW
Welcome to the big box club!
-------------------- Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
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Build a better still air box managed its cumbersome its too small, and if you cant keep it clean maybe you don't understand the concept
I work mostly with a flow hood, and its awesome, but the motions are almost identical. The only difference is you have air blowing towards you, so you have to be even more aware of your movements, as anything that comes between your work and the filter can blow contams into your sterile work, while in a still air box moving something over top your openwork might drop contams off into it, or if you stir up the air the same might happen.
That being said its easy enough to work in front of FH but if you dont like SAB work you probably wont like flow hood either
--------------------
 Amateur Mycologists United AMU Q&A
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: I HATE SAB'S! [Re: mushpunx]
#22497880 - 11/08/15 08:35 PM (8 years, 2 months ago) |
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I think hood would be easier for me. Granted I'll still need time to get the hang if it but at least nothing blocks your movement and your sight. I always end up pouring agar on the gfloor in the SAB lol. As for keeping the box clean. Umm itsa box just spray it with bleach water scrub the shit out of it with clean paper towel and respray it to work in it after a few mins. Can't really call it hard. I was really happy when I found that torn sleeve cos at least I could name the problem. Ever since I started using plastic petris my contams doubled. I should be recieving a batch of sleeves from another supplier so I might pour in those to see if they are the problem. Unless I had already thrown my agar in rhe thrash. I guess we'll see. Theres just so many things that could be causing this. I thought of the airflow since my SAB is too small.(just recently started using a glass bottle almost as tall as the SAB. maybe 1cm clearance) Thought maybe since the holes are almost at its roof level maybe the steam from agar is escaping from there making it come in from the lid at the bottom which in turn blows contams up. Even thought about that since its small my hands are creating more disruption in the air than optimal even though you have to work slow while pouring. Most of my old contams were from transfers so they probably were coming from my technique and I can live with that since it takes time to get the hang of any technique. But from pouring? Can't really fuck up 20 plates at a time just from pouring especially since all had went well in the past.
And then it's the type of contam. Usually I get a mix between trich and bacteria. Now its almost always this yellow mycellium. Driving me nuts.
I dunno hope it goes well tomorrow
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Plague_Wraith
Horseman


Registered: 07/22/15
Posts: 79
Last seen: 8 months, 20 days
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Quote:
Supalemonhaze said: I think hood would be easier for me. Granted I'll still need time to get the hang if it but at least nothing blocks your movement and your sight.
I dunno hope it goes well tomorrow
Agreed. You would definitely have more dexterity/range of movement with a flowhood. Even with just having a larger SAB that's true. It only makes sense that you would perform better (at least movement-wise) without being so constrained.
Good luck tomorrow.
-------------------- Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Argh. Darn shop opens at 8. Thought it opens at 7. Gotta wait 40 more minutes here .
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Whos the baddest bitch of them all?

The 100 litre one was really big but short. Had to buy this one. I think 2quart jars fit on each other in it. ( waitin for azur's hilarious hooker comments)
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Quote:
Pastywhyte said: If you think a flow hood is gonna be easy you might be in for a nasty surprise. If anything a flow hood is tricky because you need to have just as tight of procedure, if not better. I don't have a hood, I would like one for sure, but I am quite sure that years of toiling in a SAB will serve me well by the time I get one.
I disagree. Working with a flowhood is pretty damn easy. Yeah you need to maintain good procedure. But, keeping your paws downstream of any sterile items is pretty simple. Just keep it in your head to never pass any object between the flowhood filter and your work. If you work fairly close to the filter face it's pretty easy to manage.
Working in a SAB isn't difficult, but it is uncomfortable and the workflow blows compared to a flowhood. No gourmet operations use SABs, that I am aware of, unless it's someone just starting out. They soon realize that a SAB is only slowing them down and limiting their options. Not what you want when you're trying to run a business.
I still have my SAB, but I only use it to work with contaminated cultures if I need to.
I poured 100 petris this weekend in front of my flowhood and it was a breeze... literally.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: I HATE SAB'S! [Re: Juiceh]
#22500600 - 11/09/15 02:01 PM (8 years, 2 months ago) |
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I wasn't trying to make it sound like an SAB is better than a hood. But if people think it's anything more than an ergonomic asset they will probably be disappointed.
Main reason I have yet to aquire one is I simply do not have an appropriate place to set one up, in a room not full of carpet and all the gross stuff that comes with it. My SAB however works fine in such a space for what I need it for.
Perhaps you have need of 100 plates a week but most hobbiests do not. Nor do they need to produce 300 quarts of spawn a week. For someone like the OP, who sounds like he has yet to pull off more than a couple grows, a flow hood might be a pretty big expense especially given its size and need for a fairly dedicated space.
Given the specific nature of the OP'a situation, I would suggest sticking with the SAB for the same reason people suggest the pf Tek for noobs. Of course he may decide to get a hood and love it and all is great. But if it turns out that he has no more success with it than the box, and decides he no longer wants to pursue the hobby, then at least that's one expensive item he didn't purchase.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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I think if you stick to this hobby long enough it's worth getting a hood if the SAB is uncomfortable to work with. I guess it's just preference though. Some people are born to use a SAB and some can't use ot to save their life. So far the bigger SAB seems to be better but I guess we'll see if any of my new transfers contam. I will definitely invest in a hood in the future when I get back on my feet moneywise(regardless if I have more luck with SAB or not). Nothing massive you know just something small is enough for a hobbyist cus as you said we dont need to make a huge amount of stuff at a time.
Btw I think OP hasn't posted I a while. Might have gotten confused on that one.
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Plague_Wraith
Horseman


Registered: 07/22/15
Posts: 79
Last seen: 8 months, 20 days
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Quote:
Pastywhyte said: For someone like the OP, who sounds like he has yet to pull off more than a couple grows, a flow hood might be a pretty big expense especially given its size and need for a fairly dedicated space.
Given the specific nature of the OP'a situation, I would suggest sticking with the SAB for the same reason people suggest the pf Tek for noobs. Of course he may decide to get a hood and love it and all is great. But if it turns out that he has no more success with it than the box, and decides he no longer wants to pursue the hobby, then at least that's one expensive item he didn't purchase.

Agreed.
-------------------- Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I dunno if I was born to use a SAB but to be honest I never really had trouble with it. My biggest issue at first was not having wide enough holes cause I used a four inch diameter hole saw and my forarms were almost tight. But I got good at just using my hands and keeping my arms still so by the time I upgraded to 4.5" holes things were easy.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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I use a 5 inch hole myyself. Got some bigfoot palms here lmao
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Where did you get these agar plates from? Can you PC them? They look nice.
Quote:
Supalemonhaze said: Dont tell me bout contam issues dude. I really dont wanna hear the word agar right now. This is this weeks work all for the trash. Except for the jar. That one is older

Some of the worst contaminated plates

This week was a nightmare. I have never been so careful yet I have never experienced this much contams.
At first I thought this is happening because of a faulty sleeve

But then I made a new batch after cleaning the shit out of my SAB with bleach and soap and im still getting this fucking yellow mold. Its really starting to wear me down. For one I cannot afford to keep buying new shit every few days. And on the other hand you start to lose interest in something when all you get are losses. Worst part is I don't know wtf is this yellow piece of shit mycelium and even in contam forum they couldn't name the bitch.
Today ill go make one last buy at the supermarket for a bigger SAB but if I dont get a win soon I will throw all the agar related shit in the can. The funny thing is( makes me wanna cry tho) I knock up my jars with LC in open air and do agar in SAB. I never failed this hard with grains which make it so much worse.
I feel you man atm even looking at my SAB makes me mad as hell
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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and what variety are you using? The contam doesnt look so bad. Clean it up on a new plate. Thats what agar is for. You can't expect it to work on the first transfer.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Plates are just normal plastic plates with homemade agar. I added food coloring to see contams better. Yes true you can expect some contams but lately agar has been a bitch. That time I had the torn sleeve I only saved 2 out of 18 or so plates. Hopefully the torn sleeve plus the small SAB was the problem. I didn't used to have this many contams on agar before
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Bummer man. Wishing you luck. What type of alcohol are you using? one time I used a no name brand of disinfectant to wipe down my SAB and it caused me problems for months before I realized it was "environmentally friendly" and didn't kill bacteria worth a damn.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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I use about 15%bleach water with some soap in it. Upped it from 10% when my luck turned. Fucked up a new shirt this morning
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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a SAB is all about the still air anyways, killing 99,99% of 100 billions still = way too much nasties.
spray, dont wipe, you wanna trap the contam spores from the air to the walls of the sab.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Mist, mist, and mist again!!! You want it soggy in there.
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blackdust


Registered: 02/28/09
Posts: 8,327
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soapy water > iso plastic lids > metal lids SFD > poly no-pour > pour experience > what some crack heads recommend on the Shroomery white girls > other women GH > monos Whyte > dusty
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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I only wipe if I haven't used it in a few days. Then I respray.
Phone's about to die. See ya in about 5 hrs after work
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