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InvisibleShroomerInTheRye
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Re: I HATE SAB'S! [Re: invitro]
    #22496808 - 11/08/15 05:16 PM (8 years, 2 months ago)

To the folks who don't use a SAB, do y'all make prints?


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:nyan: <-- Clicky Clicky


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Plague_Wraith]
    #22496829 - 11/08/15 05:22 PM (8 years, 2 months ago)

Quote:

Plague_Wraith said:
Quote:

Supalemonhaze said:
Dang you have trouble sucking up LC with a 116quart SAB? Thats one of the reasons I was going to risk buying it. If agar doesn't work out at least I can do my grains and LC's in there. I am using a bigger smaller syringe though so I guess we'll see.



I know, right. Sounds like I'm handicapped. :facepalm: In truth though, I was using a half-gallon worth of LC and that particular LC jar has Polyfill stuffed in the top for use as a GE filter. That means I didn't want the polyfill getting wet at all for fear of contams getting wicked in.

I was trying to hold the jar at an angle so my 4" needle would reach the large cluster of mycellium on the bottom (didn't use a stir bar, just marbles). It was a hell of a process to get the syringe totally full. I had to actually lay the entire LC jar sideways to pull it off. Thankfully the polyfill is stuffed in there so tight nothing leaked out.

I'm sure the whole process would have been much easier if I wasn't using such massive tools (half-gallon jar, 60cc syringe, 4" dispensing needle) and if I had a proper LC filter and stir bar, but I was making do. :shrug:




My problem with LC in agar was also a big jar ( I think a little bigger than a quart) but mostly it was cos my SAB is only 50litres. Thats why I ended up doing eveything open air. Was still using micropore as a filter lmao. I think I didn't experience disaster with it because I only made it from 200ml of honey water since it was my first lc and I didn't know better. Hopefully ill have better luck working with pints and a bigger SAB. I really hope agar will work out in the end too. I think its most rewarding and fun part of mycology and a shame not to be able to do it.


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: ShroomerInTheRye]
    #22496846 - 11/08/15 05:24 PM (8 years, 2 months ago)

Quote:

ShroomerInTheRye said:
To the folks who don't use a SAB, do y'all make prints?





I did one PE print with water tek so far. Didn't use a SAB for it so im not sure if ill be able to use it if agar doesn't work out.


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Offlinespacechildo
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Re: I HATE SAB'S! [Re: ShroomerInTheRye]
    #22496857 - 11/08/15 05:26 PM (8 years, 2 months ago)

Quote:

ShroomerInTheRye said:
To the folks who don't use a SAB, do y'all make prints?




I'd be more concerned with how you gonna play with agar!
when I just take a cpl prints I just sanitize a small ice cream box and flip it over a towel. prints inside, under shotters if its pans/tiny caps species.


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OfflinePlague_Wraith
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Re: I HATE SAB'S! [Re: Supalemonhaze]
    #22496929 - 11/08/15 05:38 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:

My problem with LC in agar was also a big jar ( I think a little bigger than a quart) but mostly it was cos my SAB is only 50litres. Thats why I ended up doing eveything open air.

I really hope agar will work out in the end too. I think its most rewarding and fun part of mycology and a shame not to be able to do it.



A 50 liter SAB? Jesus, I probably would have snapped my own neck trying to fill my LC syringe in that amount of space. It'd definitely be worth an upgrade. Stealth is pretty much out the window with something around the 116 quart size though and the price is enough to make you think twice.

I've been very interested in agar lately. I can't wait to try it. I agree, it's gotta be rewarding to clone some massive shrooms or to even save a dirty culture. :laugh:


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Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Plague_Wraith]
    #22496944 - 11/08/15 05:41 PM (8 years, 2 months ago)

You should see the prices for totes in my country then :p. 50l=25euro.

Its no wonder starting mycology is the most expensive hobby I ever started.


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Offlinetetherface
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Re: I HATE SAB'S! [Re: spacechildo]
    #22496955 - 11/08/15 05:43 PM (8 years, 2 months ago)

Ive been using a 58qt SAB forever and last night I cut arm holes in a 110qt tub and its friggen great I feel like a dummy for not doing it sooner ive been doing jars 5 at a time without the proper headroom for g2g transfers now I can prolly do 20 quarts at once!! And without letting my sab settle 3-5 times per session from reloading jars/supplies big ass SAB FTW:nyan:


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: tetherface]
    #22497024 - 11/08/15 05:54 PM (8 years, 2 months ago)

Hopefully ill share your luck :smile: bout 5 more hours till the supermarket opens.


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OfflinePlague_Wraith
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Re: I HATE SAB'S! [Re: tetherface]
    #22497063 - 11/08/15 05:59 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
Its no wonder starting mycology is the most expensive hobby I ever started.



Yeah, no joke. It took me 3 years of working a miserable job to save up enough to buy the supplies I've recently acquired. Everytime I put together a list of things I need for this hobby I feel like I'm buying weird stuff too. "Yeah, gotta buy another strainer!" "Gotta buy a scalpel and a big box of garbage bags!" "Need a hole saw to drill up a tote!" :facepalm: I look at my SAB and wonder what people outside of our hobby would think of it. They'd probably be wondering what the hell was going on. :grin:

Quote:

tetherface said:
Ive been using a 58qt SAB forever and last night I cut arm holes in a 110qt tub and its friggen great I feel like a dummy for not doing it sooner ive been doing jars 5 at a time without the proper headroom for g2g transfers now I can prolly do 20 quarts at once!! And without letting my sab settle 3-5 times per session from reloading jars/supplies big ass SAB FTW:nyan:



Welcome to the big box club! :thumbup: :rockon:


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Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain


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Offlinemushpunx
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Re: I HATE SAB'S! [Re: Plague_Wraith]
    #22497091 - 11/08/15 06:03 PM (8 years, 2 months ago)

Build a better still air box managed its cumbersome its too small, and if you cant keep it clean maybe you don't understand the concept :shrug:

I work mostly with a flow hood, and its awesome, but the motions are almost identical. The only difference is you have air blowing towards you, so you have to be even more aware of your movements, as anything that comes between your work and the filter can blow contams into your sterile work, while in a still air box moving something over top your openwork might drop contams off into it, or if you stir up the air the same might happen.

That being said its easy enough to work in front of FH but if you dont like SAB work you probably wont like flow hood either


--------------------

Amateur Mycologists United
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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: mushpunx]
    #22497880 - 11/08/15 08:35 PM (8 years, 2 months ago)

I think hood would be easier for me. Granted I'll still need time to get the hang if it but at least nothing blocks your movement and your sight. I always end up pouring agar on the gfloor in the SAB lol. As for keeping the box clean. Umm itsa box just spray it with bleach water scrub the shit out of it with clean paper towel and respray it to work in it after a few mins. Can't really call it hard. I was really happy when I found that torn sleeve cos at least I could name the problem. Ever since I started using plastic petris my contams doubled. I should be recieving a batch of sleeves from another supplier so I might pour in those to see if they are the problem. Unless I had already thrown my agar in rhe thrash. I guess we'll see. Theres just so many things that could be causing this. I thought of the airflow since my SAB is too small.(just recently started using a glass bottle almost as tall as the SAB. maybe 1cm clearance) Thought maybe since the holes are almost at its roof level maybe the steam from agar is escaping from there making it come in from the lid at the bottom which in turn blows contams up. Even thought about that since its small my hands are creating more disruption in the air than optimal even though you have to work slow while pouring. Most of my old contams were from transfers so they probably were coming from my technique and I can live with that since it takes time to get the hang of any technique. But from pouring? Can't really fuck up 20 plates at a time just from pouring especially since all had went well in the past.

And then it's the type of contam. Usually I get a mix between trich and bacteria. Now its almost always this yellow mycellium. Driving me nuts.

I dunno hope it goes well tomorrow


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OfflinePlague_Wraith
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Re: I HATE SAB'S! [Re: Supalemonhaze]
    #22498126 - 11/08/15 09:36 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
I think hood would be easier for me. Granted I'll still need time to get the hang if it but at least nothing blocks your movement and your sight.

I dunno hope it goes well tomorrow



Agreed. You would definitely have more dexterity/range of movement with a flowhood. Even with just having a larger SAB that's true. It only makes sense that you would perform better (at least movement-wise) without being so constrained.

Good luck tomorrow. :thumbup:


--------------------
Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Plague_Wraith]
    #22498577 - 11/08/15 11:22 PM (8 years, 2 months ago)

Argh. Darn shop opens at 8. Thought it opens at 7. Gotta wait 40 more minutes here :zomgwtf:.


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Supalemonhaze]
    #22498721 - 11/09/15 12:14 AM (8 years, 2 months ago)

Whos the baddest bitch of them all?



The 100 litre one was really big but short. Had to buy this one. I think 2quart jars fit on each other in it. ( waitin for azur's hilarious hooker comments)


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InvisibleJuiceh
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22500441 - 11/09/15 01:13 PM (8 years, 2 months ago)

Quote:

Pastywhyte said:
If you think a flow hood is gonna be easy you might be in for a nasty surprise. If anything a flow hood is tricky because you need to have just as tight of procedure, if not better. I don't have a hood, I would like one for sure, but I am quite sure that years of toiling in a SAB will serve me well by the time I get one.



I disagree. Working with a flowhood is pretty damn easy. Yeah you need to maintain good procedure. But, keeping your paws downstream of any sterile items is pretty simple. Just keep it in your head to never pass any object between the flowhood filter and your work. If you work fairly close to the filter face it's pretty easy to manage.

Working in a SAB isn't difficult, but it is uncomfortable and the workflow blows compared to a flowhood. No gourmet operations use SABs, that I am aware of, unless it's someone just starting out. They soon realize that a SAB is only slowing them down and limiting their options. Not what you want when you're trying to run a business.

I still have my SAB, but I only use it to work with contaminated cultures if I need to.

I poured 100 petris this weekend in front of my flowhood and it was a breeze... literally.


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
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InvisiblePastywhyteMDiscord
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Re: I HATE SAB'S! [Re: Juiceh]
    #22500600 - 11/09/15 02:01 PM (8 years, 2 months ago)

I wasn't trying to make it sound like an SAB is better than a hood. But if people think it's anything more than an ergonomic asset they will probably be disappointed.

Main reason I have yet to aquire one is I simply do not have an appropriate place to set one up, in a room not full of carpet and all the gross stuff that comes with it. My SAB however works fine in such a space for what I need it for.

Perhaps you have need of 100 plates a week but most hobbiests do not. Nor do they need to produce 300 quarts of spawn a week. For someone like the OP, who sounds like he has yet to pull off more than a couple grows, a flow hood might be a pretty big expense especially given its size and need for a fairly dedicated space.

Given the specific nature of the OP'a situation, I would suggest sticking with the SAB for the same reason people suggest the pf Tek for noobs. Of course he may decide to get a hood and love it and all is great. But if it turns out that he has no more success with it than the box, and decides he no longer wants to pursue the hobby, then at least that's one expensive item he didn't purchase.

:twocents:


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22500636 - 11/09/15 02:13 PM (8 years, 2 months ago)

I think if you stick to this hobby long enough it's worth getting a hood if the SAB is uncomfortable to work with. I guess it's just preference though. Some people are born to use a SAB and some can't use ot to save their life. So far the bigger SAB seems to be better but I guess we'll see if any of my new transfers contam. I will definitely invest in a hood in the future when I get back on my feet moneywise(regardless if I have more luck with SAB or not). Nothing massive you know just something small is enough for a hobbyist cus as you said we dont need to make a huge amount of stuff at a time.

Btw I think OP hasn't posted I a while. Might have gotten confused on that one.


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OfflinePlague_Wraith
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22500654 - 11/09/15 02:21 PM (8 years, 2 months ago)

Quote:

Pastywhyte said:
For someone like the OP, who sounds like he has yet to pull off more than a couple grows, a flow hood might be a pretty big expense especially given its size and need for a fairly dedicated space.

Given the specific nature of the OP'a situation, I would suggest sticking with the SAB for the same reason people suggest the pf Tek for noobs. Of course he may decide to get a hood and love it and all is great. But if it turns out that he has no more success with it than the box, and decides he no longer wants to pursue the hobby, then at least that's one expensive item he didn't purchase.

:twocents:



Agreed. :thumbup:


--------------------
Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain


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InvisiblePastywhyteMDiscord
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Re: I HATE SAB'S! [Re: Supalemonhaze]
    #22500658 - 11/09/15 02:23 PM (8 years, 2 months ago)

I dunno if I was born to use a SAB but to be honest I never really had trouble with it. My biggest issue at first was not having wide enough holes cause I used a four inch diameter hole saw and my forarms were almost tight. But I got good at just using my hands and keeping my arms still so by the time I upgraded to 4.5" holes things were easy.


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22501029 - 11/09/15 04:04 PM (8 years, 2 months ago)

I use a 5 inch hole myyself. Got some bigfoot palms here lmao


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