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OfflineTriggerFish
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Registered: 09/23/15
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Re: I HATE SAB'S! [Re: donkeycong]
    #22495601 - 11/08/15 01:05 PM (8 years, 2 months ago)

Quote:

donkeycong said:

My latest failure haha:






Well, I know one thing that could help you. Having petri dishes sit on that uneven plastic on the bottom can definitely result in catastrophe as its not the most stable surface, especially when your arms are moving the box around.

Turn the box over, don't use the lid. So even if your box moves around a little, the items you have in the box are still on a non moving surface such as the table you are working on. Vision through the bottom of the box could be an issue, so I cut a huge rectangle out of the bottom and hot glued a nice piece of thick transparent plastic in place of it. I also added a line of hot glue all around the top edge (bottom when u are working) of the box, so it sorta holds to the table when I'm working.

Capisce?


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InvisiblePastywhyteMDiscord
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Re: I HATE SAB'S! [Re: Arush]
    #22495605 - 11/08/15 01:06 PM (8 years, 2 months ago)

Quote:

Arush said:
I know, still less of a chance imo. Plus you can make your syringes very dark giving you way more innoc points.




:justno:


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OfflineArush
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22495776 - 11/08/15 01:46 PM (8 years, 2 months ago)

:freewilly:


--------------------
http://www.shroomery.org/forums/showflat.php/Number/22409475
โ€œPsychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behaviour and information processing. They open you up to the possibility that everything you know is wrong.โ€
โ€• Terence McKenna


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OfflineLogicaL ChaosM
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Re: I HATE SAB'S! [Re: donkeycong]
    #22496243 - 11/08/15 03:25 PM (8 years, 2 months ago)

I would use different gloves, ones with long cuffs. Your skin could be  getting into the air,  and skin has lots of microbes growing on it.

Also, your walls are dry. They should be coated with soapy or bleachy water.


As for a hard surface. I would recommend buying a smaall piece of thick, heavy plywood, wrap it something non porus like a thick garbage bag, taping it tight, and put that inside thee bottom of the  SAB. Make sure it fits inside the bottom of the SAB in the best fit as possible.

All these should help u out.


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: I HATE SAB'S! [Re: Arush]
    #22496277 - 11/08/15 03:34 PM (8 years, 2 months ago)

Dont tell me bout contam issues dude. I really dont wanna hear the word agar right now. This is this weeks work all for the trash. Except for the jar. That one is older


Some of the worst contaminated plates



This week was a nightmare. I have never been so careful yet I have never experienced this much contams.

At first I thought this is happening because of a faulty sleeve


But then I made a new batch after cleaning the shit out of my SAB with bleach and soap and im still getting this fucking yellow mold. Its really starting to wear me down. For one I cannot afford to keep buying new shit every few days. And on the other hand you start to lose interest in something when all you get are losses. Worst part is I don't know wtf is this yellow piece of shit mycelium and even in contam forum they couldn't name the bitch.

Today ill go make one last buy at the supermarket for a bigger SAB but if I dont get a win soon I will throw all the agar related shit in the can. The funny thing is( makes me wanna cry tho) I knock up my jars with LC in open air and do agar in SAB. I never failed this hard with grains which make it so much worse.

I feel you man atm even looking at my SAB makes me mad as hell


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InvisiblePastywhyteMDiscord
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Re: I HATE SAB'S! [Re: Supalemonhaze]
    #22496351 - 11/08/15 03:52 PM (8 years, 2 months ago)

Try no pour. Cheaper and easier than pour IMO.


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22496372 - 11/08/15 03:57 PM (8 years, 2 months ago)

Dont get those kinda bowls here. The ones we do get cost as much as a sleeve per bowl. Not really worth it.

Edit: meh if I lived in the US I wouldn't have spent half of what I already did to get this far. And I really didn't get far as of yet


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InvisiblePastywhyteMDiscord
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Re: I HATE SAB'S! [Re: Supalemonhaze]
    #22496378 - 11/08/15 03:59 PM (8 years, 2 months ago)

Use jars. I assume you have jars. Keep in mind a methods worth is dependent on if it works or not.


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22496410 - 11/08/15 04:05 PM (8 years, 2 months ago)

Got pint jars. Only 7 wide mouth though that are short enough. Rest are ball which are kinda tall to work with.

I dunno If I can't get it to work with what I have I am definitely stopping there as far as agar goes. Sucks but anymore of this and ill go crazy.

Do you know what kinda mold it is by the way? That's what driving me nuts most.


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OfflinePlague_Wraith
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Registered: 07/22/15
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Re: I HATE SAB'S! [Re: Supalemonhaze]
    #22496446 - 11/08/15 04:11 PM (8 years, 2 months ago)

I hate using a SAB as well. :smokerage: I don't know that I can accurately convey my rage.

I went out of my way to purchase a 116 quart Sterilite tote and staggered the arm holes using a hole saw set for comfort. It is still an unbelievably clunky process to use it. Even the process of filling a syringe with LC inside of there was enough to make me want to throw it off the deck. I cut 4" holes and my arms ended up getting stuck as well. Talk about pissing me off. :mad2: I tried knocking up grain jars with a 60cc syringe with a 4 inch, 14 gauge dispensing needle in there too. Screw trying to do that for 11 jars. I ended up doing half of them in open air. Next time I'm going totally open air. I'll recut the holes at 5" and try it again for agar in a week. So far I've had zero contams in open air.

Stupid SAB. :whippedcreamhead:  :cantwashthataway:  :drunkdriver:  :suckmydickbitches:


--------------------
Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain


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OfflinecronicrFacebook
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22496543 - 11/08/15 04:31 PM (8 years, 2 months ago)

Quote:

Pastywhyte said:
Try no pour. Cheaper and easier than pour IMO.



i just poured no pours like petri's last night:mindblown:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePastywhyteMDiscord
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Re: I HATE SAB'S! [Re: cronicr]
    #22496547 - 11/08/15 04:32 PM (8 years, 2 months ago)

Quote:

cronicr said:
Quote:

Pastywhyte said:
Try no pour. Cheaper and easier than pour IMO.



i just poured no pours like petri's last night:mindblown:




:brilliant:


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InvisibleSupalemonhaze
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Re: I HATE SAB'S! [Re: Plague_Wraith]
    #22496549 - 11/08/15 04:33 PM (8 years, 2 months ago)

Knocking up jars in open air is not so bad if you do it right. I tilt the jar slightly and peel back the tyvek just enough to see the hole. The tilt and position of the jar makes sure that the tyvek still covers the hole from above. Then I insert the syringe and cover that with the tyvek while I  shoot LC in it real fast. Dont have many contam issues this way.

Dang you have trouble sucking up LC with a 116quart SAB? Thats one of the reasons I was going to risk buying it. If agar doesn't work out at least I can do my grains and LC's in there. I am using a bigger smaller syringe though so I guess we'll see.


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OfflinecronicrFacebook
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22496551 - 11/08/15 04:33 PM (8 years, 2 months ago)



--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineDrCrumbs
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Re: I HATE SAB'S! [Re: Plague_Wraith]
    #22496566 - 11/08/15 04:36 PM (8 years, 2 months ago)

You could try lowering your PH to around 5.6 so your medium is more selective for myc instead of bacteria.


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InvisiblePastywhyteMDiscord
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Re: I HATE SAB'S! [Re: cronicr]
    #22496579 - 11/08/15 04:37 PM (8 years, 2 months ago)

Quote:

cronicr said:





Nice. Might try that next time I need to hot pour.


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OfflinecronicrFacebook
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Re: I HATE SAB'S! [Re: Pastywhyte]
    #22496597 - 11/08/15 04:40 PM (8 years, 2 months ago)

was a total accident lol, was just going to run them through the pc because they were all contaminated  plates and i i figured i would clean them before putting them away and was running the pc for agar anyway....pulled the agar out and 3 jars with 10 salad cups in each and thought"why not just pour these fuckers" lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePastywhyteMDiscord
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Re: I HATE SAB'S! [Re: cronicr]
    #22496606 - 11/08/15 04:42 PM (8 years, 2 months ago)

Lol sounds like you :wink:


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OfflinePlague_Wraith
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Re: I HATE SAB'S! [Re: Supalemonhaze]
    #22496775 - 11/08/15 05:11 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
Dang you have trouble sucking up LC with a 116quart SAB? Thats one of the reasons I was going to risk buying it. If agar doesn't work out at least I can do my grains and LC's in there. I am using a bigger smaller syringe though so I guess we'll see.



I know, right. Sounds like I'm handicapped. :facepalm: In truth though, I was using a half-gallon worth of LC and that particular LC jar has Polyfill stuffed in the top for use as a GE filter. That means I didn't want the polyfill getting wet at all for fear of contams getting wicked in.

I was trying to hold the jar at an angle so my 4" needle would reach the large cluster of mycellium on the bottom (didn't use a stir bar, just marbles). It was a hell of a process to get the syringe totally full. I had to actually lay the entire LC jar sideways to pull it off. Thankfully the polyfill is stuffed in there so tight nothing leaked out.

I'm sure the whole process would have been much easier if I wasn't using such massive tools (half-gallon jar, 60cc syringe, 4" dispensing needle) and if I had a proper LC filter and stir bar, but I was making do. :shrug:


--------------------
Anger is an acid that can do more harm to the vessel in which it is stored than to anything on which it is poured. - Mark Twain


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Offlineinvitro

Registered: 05/03/13
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Re: I HATE SAB'S! [Re: Plague_Wraith]
    #22496801 - 11/08/15 05:16 PM (8 years, 2 months ago)

Quote:

Plague_Wraith said:
I hate using a SAB as well. :smokerage: I don't know that I can accurately convey my rage.

I went out of my way to purchase a 116 quart Sterilite tote and staggered the arm holes using a hole saw set for comfort. It is still an unbelievably clunky process to use it. Even the process of filling a syringe with LC inside of there was enough to make me want to throw it off the deck. I cut 4" holes and my arms ended up getting stuck as well. Talk about pissing me off. :mad2: I tried knocking up grain jars with a 60cc syringe with a 4 inch, 14 gauge dispensing needle in there too. Screw trying to do that for 11 jars. I ended up doing half of them in open air. Next time I'm going totally open air. I'll recut the holes at 5" and try it again for agar in a week. So far I've had zero contams in open air.

Stupid SAB. :whippedcreamhead:  :cantwashthataway:  :drunkdriver:  :suckmydickbitches:




I feel the same way, I used a sab for about 2 years but in the past year? I used it once, and that may be my last time. I hate doing sab work with a passion, I don't know why I just feel very restricted and clumsy in them.  I had a large tote, armholes spaced apart nicely, still hated it.


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