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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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I HATE SAB'S!
#22495373 - 11/08/15 11:59 AM (8 years, 2 months ago) |
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Whenever I use one, i work meticulous to keep everything as clean as possible. Half the time the projects, whether it be G2G or agar work, end up all over the bottom as I have never had to do anything more cumbersome in my life. The other half of time when the projects don't end up all over the place, my hard work and time results in contaminates anyway. I think I'm going back to old school syringe to grain jars until I can afford a flow-hood. Just a rant
My latest failure haha:
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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If you think a flow hood is gonna be easy you might be in for a nasty surprise. If anything a flow hood is tricky because you need to have just as tight of procedure, if not better. I don't have a hood, I would like one for sure, but I am quite sure that years of toiling in a SAB will serve me well by the time I get one.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Pasty plates are nice in a SAB cause they don't spill 
I feel your pain, i placed my arm holes too close together so now i feel like im working with handcuffs on 
Even doing pf-tek i have spilt two jars all over the bottom!
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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Very true. I think my best bet would be to master the SAB, but I need to toss this crappy one and make one way bigger so Its at least somewhat manageable!
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Do a couple dry runs to get your technique down..that way you know exactly what and how you are going to do it...otherwise you are just freestyle SAB working, and all sorts of unforseen crap happens.
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Q&A US vs. THEM The more I learn, the less I know.
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ShroomerInTheRye
Clit Commander



Registered: 01/12/12
Posts: 13,036
Loc: Themyscira
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I'm curious...are you working in a room with a vent for the AC?
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<-- Clicky Clicky
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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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Yea but i make sure to turn it off at least an hour prior or so. My problem most likely stems from having 6 pets though I wouldn't wanna know whats floating around in this house
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DaveyJones6911
Nonconformist


Registered: 09/08/15
Posts: 690
Loc: EU
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there's an easier way to work with agar: http://www.shroomery.org/forums/showflat.php/Number/19208976
i tried petrie dishes because someone gave me a bunch of them a long time ago, but i too found them difficult to handle. the tek in the link offers an option where the agar has solidified before it gets to the SAB.
i have found that to be very helpful.
-------------------- Creativity is a dangerous thing in the eyes of those who don't have any.
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StickyIcky Fingers
Free DOOM!! Available Here

Registered: 12/03/13
Posts: 178
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When I started using one practicing what I was going to be doing helped a lot. I would put everything in and go through the motions without opening anything before spraying the SAB and actually doing the clean work. It helped me see what the hard parts were going to be instead of trying to figure out what order to do things in and trying to maintain decent sterile technique. It helped me to get my tools and jars lined up and in an accessible part of the SAB so I could reach everything I needed to use with whatever hand was going to need it.
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TheScientificMethod
Psychonautic Explorer & Writer



Registered: 02/20/14
Posts: 632
Loc: USA
Last seen: 21 days, 20 hours
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I have to agree with you OP. I used to use SABs and they were more of a pain in the ass than anything else. In fact I almost lit my house on fire once because of an SAB. I was trying to be a sterile as possible and so I had a rag soaked in alcohol in my SAB and I flamed a needle, only to set the rag, then the whole box on fire (Stupid, I know, but I've learned my lesson). The entire box turned into a ball of fire and my hands were stuck inside, so I frantically was trying to get my hands free, flung the box on the carpet and almost set the carpet on fire... that was a scary moment.
Anyways, since that day I have forgone the SAB and created a Still Air LAB. I literally set up my old MMJ grow tent (4x6x8'). I clean the hell out of the inside the night before then lysol the entire thing. I make it as clean as possible, then I pressure cook/sterilize a tyvec suit and mask. So when I go in there I'm as clean as possible. That way I can work without restriction to my movement. I've found this to work a lot better, but I still do deal with contamination from time to time.
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ShroomerInTheRye
Clit Commander



Registered: 01/12/12
Posts: 13,036
Loc: Themyscira
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I live in a tiny little apartment with 2 large cats. I only have a 6' x 2' space to work in. I hung a white shirt over the vent and closed the shirt holes with a hair clip. Honestly, it was disgusting how fast that shirt turned yellow, and I don't smoke inside. Smelled awful too. I sent some prints off a few weeks ago taken in my SAB, and they turned up with no contams.
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<-- Clicky Clicky
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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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Wow that sounds traumatizing! glad your alright! Love the idea of a SAL btw, the SAB just isn't big enough for efficient work. I've never had a part of the hobby i've disliked as much as working in these things
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Get a bigger sab op
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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donkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
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I have to try this now, scared what the results might be though. So much dust, and I smoke inside aha
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candela
Stranger


Registered: 09/04/15
Posts: 281
Last seen: 2 years, 6 months
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Dude, I am totally new to this hobby (2 months) just like you. I have already knocked up over 100 jars without any contams yet, by just using an SAB.
What I do: I literally spray the inside of my SAB with Alcohol 70% till its dripping on the walls (Dont wipe it dry!). Leave the inside of the SAB soaked, and dripping with alcohol while your doing your transfers and inoculations.
I maybe new to this and I don't know if this may be some good advice to give to you, but it has worked well for me so far.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: I HATE SAB'S! [Re: candela]
#22495581 - 11/08/15 12:58 PM (8 years, 2 months ago) |
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Be careful with that much iso and a flame source/hot tools. It can lead to 
Bleach or soapy water really do work good for a SAB and you are much more fire marshal friendly
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Arush
Godfather



Registered: 05/06/13
Posts: 312
Loc: Canada
Last seen: 5 years, 9 months
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Personally I think spore syringes are easier. It may take 5 days longer for the spores to start growing but much less chance of contams. Cap>print>syringe>inoculate. Also you don't have to use petri dishes.. half pint jars would as well and won't over flow.
-------------------- http://www.shroomery.org/forums/showflat.php/Number/22409475 “Psychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behaviour and information processing. They open you up to the possibility that everything you know is wrong.” ― Terence McKenna
Edited by Arush (11/08/15 01:02 PM)
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candela
Stranger


Registered: 09/04/15
Posts: 281
Last seen: 2 years, 6 months
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Quote:
Toadstool5 said: Be careful with that much iso and a flame source/hot tools. It can lead to 
Bleach or soapy water really do work good for a SAB and you are much more fire marshal friendly 
Yes. Place your flame source (alcohol lamp) far away, but still within arms length from your SAB.
Edited by candela (11/08/15 01:04 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: I HATE SAB'S! [Re: Arush]
#22495593 - 11/08/15 01:03 PM (8 years, 2 months ago) |
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Quote:
Arush said: Personally I think spore syringes are easier. It may take 5 days longer for the spores to start growing but much less chance of contams. Cap>print>syringe>inoculate.
Unless of course the spore syringe is full of contams. Which happens a lot. In fact it's pretty much impossible to guarantee a syringe won't have any contams at all.
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Arush
Godfather



Registered: 05/06/13
Posts: 312
Loc: Canada
Last seen: 5 years, 9 months
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I know, still less of a chance imo. Plus you can make your syringes very dark giving you way more innoc points.
-------------------- http://www.shroomery.org/forums/showflat.php/Number/22409475 “Psychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behaviour and information processing. They open you up to the possibility that everything you know is wrong.” ― Terence McKenna
Edited by Arush (11/08/15 01:04 PM)
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