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OfflineBigbadwooof
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Noc'd LC while still hot :(
    #22493141 - 11/07/15 09:30 PM (8 years, 2 months ago)

So, I was being a mindless mushroom drone, and I pulled my LC jar straight out of the PC, infront of the flowhood, and inoculated it with a wedge.

It was still hot, and I realized what I had done afterwords when I picked up the jar lmao!

I quickly put it in a cool water bath, and it cooled down within maybe 10 minutes.

Thoughts?


--------------------
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InvisibleJuiceh
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Re: Noc'd LC while still hot :( [Re: Bigbadwooof]
    #22493145 - 11/07/15 09:31 PM (8 years, 2 months ago)

Drop in another wedge.


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OfflineBigbadwooof
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Re: Noc'd LC while still hot :( [Re: Juiceh]
    #22493169 - 11/07/15 09:37 PM (8 years, 2 months ago)

Quote:

Juiceh said:
Drop in another wedge.




I don't have another wedge. This wedge has been sitting in a micro-centrifuge tube, because I was going to give it to a buddy. I thought I had this clone on a slant. I just fruited it and holy DAMN it's an amazing PE clone!

I had 2 wedges in the tube, and one is on agar now also, so all is not lost! I am just curious if anyone has had any positive experiences with this situation lol!


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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OfflinePlague_Wraith
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Re: Noc'd LC while still hot :( [Re: Bigbadwooof]
    #22493570 - 11/07/15 11:34 PM (8 years, 2 months ago)

I made an LC using spores a while back and did pretty much the same thing. The jar wasn't immensely hot, but definitely uncomfortable to handle even when holding it with a dish towel. I figured everything was dead and kind of shamed myself for rushing my work, but everything turned out. That particular batch of LC has been damn good too. It's been sitting at room temperature for at least 5 months with a polyfill filter and it's still pure and usable. I've even laid it on it's side to fill a syringe in open air and it still hasn't contaminated. If that's not a miracle LC, I don't know what is.

Probably give it a week and see what it does. You'll know if it made it or not.


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OfflineBigbadwooof
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Re: Noc'd LC while still hot :( [Re: Plague_Wraith]
    #22493615 - 11/07/15 11:47 PM (8 years, 2 months ago)

Quote:

Plague_Wraith said:
I made an LC using spores a while back and did pretty much the same thing. The jar wasn't immensely hot, but definitely uncomfortable to handle even when holding it with a dish towel. I figured everything was dead and kind of shamed myself for rushing my work, but everything turned out. That particular batch of LC has been damn good too. It's been sitting at room temperature for at least 5 months with a polyfill filter and it's still pure and usable. I've even laid it on it's side to fill a syringe in open air and it still hasn't contaminated. If that's not a miracle LC, I don't know what is.

Probably give it a week and see what it does. You'll know if it made it or not.




Thanks for the input, brother. Unfortunately, I think this is a bit of a different ballgame than spores, but maybe not! I would imagine starting an LC from spores would only benefit from the heat if the spores managed to survive, because it might hinder potential contaminants.

We will see! I will post back with the results for future reference. I have a feeling it will be fine :wink:


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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InvisibleMad Season
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Re: Noc'd LC while still hot :( [Re: Bigbadwooof]
    #22493639 - 11/07/15 11:59 PM (8 years, 2 months ago)

I bet it'll be fine. Myc is quite tough! Also hot pouring agar over myc infected with bacteria is an acceptable practice. Hot agar isn't much different


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OfflineBigbadwooof
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Re: Noc'd LC while still hot :( [Re: Mad Season]
    #22493663 - 11/08/15 12:11 AM (8 years, 2 months ago)

That's what I was thinking, but how hot do you do hot-pours? This must have been near 200F.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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InvisibleCitric
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Re: Noc'd LC while still hot :( [Re: Bigbadwooof]
    #22493976 - 11/08/15 01:39 AM (8 years, 2 months ago)

Quote:

Bigbadwooof said:
So, I was being a mindless mushroom drone, and I pulled my LC jar straight out of the PC, infront of the flowhood, and inoculated it with a wedge.

It was still hot, and I realized what I had done afterwords when I picked up the jar lmao!

I quickly put it in a cool water bath, and it cooled down within maybe 10 minutes.

Thoughts?




Well, you opened the jar and it didn't sink in then that it was hot, so couldn't of been to bad.  Myc can be very forgiving and take one hell of a beating.  I'll place my bet on it's fine, may just need a little extra TLC and recovery time.


--------------------
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InvisibleSupalemonhaze
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Re: Noc'd LC while still hot :( [Re: Mad Season]
    #22494197 - 11/08/15 03:26 AM (8 years, 2 months ago)

I thought that myc dies at 100+ degrees. Mycelium impresses me everyday


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Offlineblackout
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Re: Noc'd LC while still hot :( [Re: Bigbadwooof]
    #22495439 - 11/08/15 12:18 PM (8 years, 2 months ago)

Quote:

Bigbadwooof said:
That's what I was thinking, but how hot do you do hot-pours? This must have been near 200F.



A hot/warm pour will have far less energy involved. A low volume of liquid vs a jar full

This is RR talking of them

Quote:

One of my other cultures from a wild print that I thought would never get clean enough to use has a little fruit forming invitro in its petri dish this morning. The hot agar did the trick. I simply poured the hot antibiotic agar right on top of the mycelium in the contaminated petri dish. When it grew up through the top layer, it made a little invitro mushroom. Now I have something clean to clone/transfer. It gives me hope for the other project as well.

By the way, the hot agar trick proves 106F isn't thermal death to mycelium. The agar was barely cool enough to handle the bottle without a glove when I poured it. I'd estimate around 120-130F.




The 106F figure is no doubt taken from TMC, but this is presumably long term exposure, just like with pressure cooking we maintain 121C for x minutes. Stamets never said the moment it reaches 106F it instantly dies. Just like he never recommended incubating at 86F.

If you are pouring warm agar on a plate then the thin layer will cool down very quickly, the layer hitting the agar will be chilled very fast and will solidify and stop the warmer agar above from readily mixing downwards.

When you put the LC in the water bath did you swirl it around, this would have a much faster cooling effect.


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OfflineBigbadwooof
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Re: Noc'd LC while still hot :( [Re: blackout]
    #22495750 - 11/08/15 01:42 PM (8 years, 2 months ago)

Yes, this is why I think it is a bit different than hot-pouring plates. The agar cool a lot faster when you're using such a thin layer. I immediately put the LC jar in a cool water bath, but it takes time to cool a liquid, even in a water bath.

I also put it on my stir plate after roughly 10 minutes, so it got a nice stir. I will be sure to report back! Ty for the input! :smile:


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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