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OfflineGroosBrewz
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Registered: 01/10/11
Posts: 123
Last seen: 3 years, 10 months
Colonized bird seed to agar petri?
    #22491789 - 11/07/15 04:44 PM (8 years, 2 months ago)

Hey all,

Just wondering if it's possible to take a single colonized piece of spawn (bird seed or the grain) and place on agar for storage purposes.. I did this once before years ago and did get a culture (pearl oyster) but it was very thin and wispy.. Thoughts? Seems quicker than waiting to tissue culture and actual mushroom that hasn't been,grown yet.


--------------------
"It's better to have it and not need it than need it and not have it" -- Captain Call

"American beer is like making love in a canoe- it's f@#%ing close to water!" Eric Idle


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Invisibledreadhed
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Registered: 01/21/11
Posts: 175
Re: Colonized bird seed to agar petri? [Re: GroosBrewz]
    #22492170 - 11/07/15 05:49 PM (8 years, 2 months ago)

Yes that'll work. Just have to be sterile.


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OfflineGr0wer
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Registered: 09/16/03
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Re: Colonized bird seed to agar petri? [Re: dreadhed]
    #22492918 - 11/07/15 08:38 PM (8 years, 2 months ago)

ive checked spawn before like that. Just a few grains onto agar and make sure the growth has no bacteria or yeasts growing on it. I would only do this after a few generations of not being on agar to verify the culture is still pure. One issue with long term storage on agar is it dries out. Pour your plates thick if you want to store for long periods.


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OfflineGroosBrewz
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Registered: 01/10/11
Posts: 123
Last seen: 3 years, 10 months
Re: Colonized bird seed to agar petri? [Re: Gr0wer]
    #22496177 - 11/08/15 03:13 PM (8 years, 2 months ago)

Thanks for the info!


--------------------
"It's better to have it and not need it than need it and not have it" -- Captain Call

"American beer is like making love in a canoe- it's f@#%ing close to water!" Eric Idle


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