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Offlinetorpy
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Registered: 09/09/15
Posts: 38
Last seen: 7 years, 11 months
Contaminated room?
    #22487966 - 11/06/15 07:45 PM (8 years, 2 months ago)

I have tried to fruit 3 different monos in a room, every single one has gone green on me. They have all been 100% colonized and I am positive at least 2 were made with clean spawn and decent genetics.

All on bucketed C/V/G, ive never had a problem with it going green.

Is the room fucked? I thought since my subs were at 100 they would at least push out a flush before getting wrecked


Ive got some colonizing in a different room and they wont be going anywhere near that place of evil


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OfflineMachiavelliavore
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Re: Contaminated room? [Re: torpy]
    #22487976 - 11/06/15 07:49 PM (8 years, 2 months ago)

It's probably your spawn.  Coir really doesn't contam of its own valition.  Try taking a quart of buckettek coir and leaving it in the room for a couple weeks with a lid over it, see what grows if you want to convince yourself.

Both mold and bacteria can be quite adept at sneaking through in spawn.  The way you describe it, it sounds more like mold.  You can usually get some results out of bacterial spawn before it assplodes.

Was it really hot when you lost these tubs?


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (11/06/15 07:49 PM)


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Offlinetorpy
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Registered: 09/09/15
Posts: 38
Last seen: 7 years, 11 months
Re: Contaminated room? [Re: Machiavelliavore]
    #22488260 - 11/06/15 08:55 PM (8 years, 2 months ago)

My spawn is always white and mush smelling, its appears fine and colonizes the sub quickly, then when introduced to fruiting conditions it goes green.

It probably is my spawn but could the room also be contributing?


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Offlinethebug76
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Re: Contaminated room? [Re: torpy]
    #22488279 - 11/06/15 08:58 PM (8 years, 2 months ago)

Not very probable that early. Also, if a room in your house has a heavy spore load, it's likely your entire house does; just opening a door creates currents that will circulate the spores around.


--------------------
Bug 


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OfflineMachiavelliavore
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Re: Contaminated room? [Re: torpy]
    #22488283 - 11/06/15 08:59 PM (8 years, 2 months ago)

Quite doubtful.  Mold can be quite sneaky.  If it's very low by proportion you may not see it or smell it.

Mold spores on coir won't do much if anything.  But when you put mold colonized grain into coir, holy fuck that shit's fueled up and ready to run with it in a new land of hydrated opportunity.

Most of the spore load in your tub is also probably coming from where it was spawned not the room it's sitting in/fruiting in.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: Contaminated room? [Re: torpy]
    #22488321 - 11/06/15 09:07 PM (8 years, 2 months ago)

It's your spawn. And if its white I'd expect it to smell like mushies.. They're quite strong smelling. If you'd like some links I'd be glad to help :smile:.

Ah fuck it I'm bored. Heres some links:

http://www.shroomery.org/forums/showflat.php/Number/22020260 read the first part in the white molds section. It talks about smelling

http://www.shroomery.org/forums/showflat.php/Number/22255723 this shows how trich is sneaky AF and won't show until its stressed (spawning a trichy jar or shaking and recovering before spawning)


http://www.shroomery.org/forums/showflat.php?Cat=&Board=255&Number=17165834&PHPSESSID=&fpart=1#17165834 this is a fuckin fantastic link

Also bacteria can weaken the myc, and make it easier to germinate trich on
Quote:

Mad Season said:
Quote:

Mad Season said:
http://www.shroomery.org/forums/showflat.php/Number/21890806#21890806 bacteria in spawn. You can also get it from prepping the substrate too wet. If you read in the link it explains what happened when I spawned bacterial spawn.



http://www.shroomery.org/forums/remlinker.php?Cat=&Entry=149430&F_Board=22&Thread=21936821&Main=21936535

Read both of those thoroughly.




--------------------
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Offlinetorpy
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Registered: 09/09/15
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Last seen: 7 years, 11 months
Re: Contaminated room? [Re: Mad Season]
    #22488354 - 11/06/15 09:12 PM (8 years, 2 months ago)

thank you sirs!

I just got into agar so im hoping I can cut this shit out


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InvisibleDampMushroom
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Registered: 12/01/14
Posts: 115
Re: Contaminated room? *DELETED* [Re: Mad Season]
    #22488374 - 11/06/15 09:15 PM (8 years, 2 months ago)

Post deleted by DampMushroom

Reason for deletion: I've got to leave for a couple years. Hope the best for all of y'all.



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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: Contaminated room? [Re: DampMushroom]
    #22488989 - 11/07/15 12:38 AM (8 years, 2 months ago)

Voracious environmental contamination is a real thing.  I wish the forums took it more seriously.

Torpy, tell us about your place.  The house itself (age, floors, problems, upkeep?) and whereabouts you live (desert, forested, rivers, city, suburban, southern, northern, eastern, western?)


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

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The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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InvisibleMad Season
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Re: Contaminated room? [Re: Violet]
    #22489960 - 11/07/15 10:06 AM (8 years, 2 months ago)

It is? I don't ever say I got a contam from my room. If it was truly environmental how did I do open air grows, and how do I have success? Ive had my fair share of contams..

like these. But they were from bacterial spawn.


Even after 5 years I'm not entirely confident in my spawn. Clean spawn is literally the hardest thing you can do in this hobby.

Also I've left pasteurized manure in open air in my basement for 3 months. It didn't have any molds or contams on it. But suddenly adding grains makes it contaminate.. Sorry but I 110% believe contams come from spawn. Bacterial spawn might make it have a contam that came from the environment.. But that's still the spawns fault. I've done the pasteurized manure left out experiment a good 5 times now, none of them have contaminated. I can even take a PIC of the shit I've got going right now that's a couple months old.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (11/07/15 10:09 AM)


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Offlinetorpy
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Registered: 09/09/15
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Re: Contaminated room? [Re: Mad Season]
    #22490153 - 11/07/15 10:45 AM (8 years, 2 months ago)

The house is pretty clean, but ive accidentally disturbed some trich and I figured the room is covered in spores by now..

I do believe it is my spawn, I recently lost 40+ jars to bacteria and other nasties. It was all G2G so I figure that was it not my tech, ive got a decent SAB.

@DampMushroom when you say glovebox do you mean sealed and sterile or a SAB?

I found a contammed jar with little invitro pins today, would it be possible to clone them?

http://imgur.com/a/pgdkE
http://imgur.com/a/pgdkE


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InvisiblePastywhyteMDiscord
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Re: Contaminated room? [Re: torpy]
    #22490461 - 11/07/15 11:54 AM (8 years, 2 months ago)

Certain contaminates are definitely an issue regarding spore loads of your grow space. I have battled white molds for years, was the first contamination I ever had and the only one I worry about regularly. They can set in during fruiting and for that reason are no joke. Certain parts of the world are more prone as well. Pennsylvania has a major problem with it, almost wiped their agaricus industry out several times.


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InvisibleViolet
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Re: Contaminated room? [Re: Pastywhyte]
    #22490643 - 11/07/15 12:39 PM (8 years, 2 months ago)

Yep.
I had a long-running apergillus problem that could only be fixed by inoculating my bulk substrate inside its treatment bags, effectively limiting me only to blocks instead of tubs.  I actually preferred the change. But even afterwards, the places I've lived that were both older buildings and nearer to water in the forests continued to have a quick loss to those contams.
The problem is also part of what spurred me to develop my methods.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflineMachiavelliavore
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Re: Contaminated room? [Re: torpy]
    #22491338 - 11/07/15 03:16 PM (8 years, 2 months ago)

Quote:

torpy said:
The house is pretty clean, but ive accidentally disturbed some trich and I figured the room is covered in spores by now..

I do believe it is my spawn, I recently lost 40+ jars to bacteria and other nasties. It was all G2G so I figure that was it not my tech, ive got a decent SAB. 




Dunno if you're using agar, but I highly suggest using it if you plan to G2G.  Shit can get out of control quickly with all that expansion.  If not, at least try dropping spores onto BRF or conrmeal plates to make your masters, and transfer far from the incoulation point so that, hopefully, anything hiding under the mycellium doesn't get transfered.

How are your PC times/jar loads?

I've let several tubs go pure green in my grow space.  I believe Azur said he has as well.  Perhaps I've underestimated environmental contams, but I don't think trich is one of them.  If the trich spore load is heavy that should just means anything weak in your grow space might go a bit faster than you would expect.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinetorpy
Stranger

Registered: 09/09/15
Posts: 38
Last seen: 7 years, 11 months
Re: Contaminated room? [Re: Machiavelliavore]
    #22491387 - 11/07/15 03:25 PM (8 years, 2 months ago)

swabbed plates today, diving into agar fully to best eliminate contams.

I usually load 7 jars 15psi for 90min


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