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OfflineYaMoonSun
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Inoculation through polyfill
    #22486841 - 11/06/15 03:50 PM (8 years, 2 months ago)

If the polyfill has been exposed to open air because I foolishly removed the foil a bit early, is it just going to dirty the sterile needle as it passes through the polyfill and ruin my agar plate?


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OfflinePsilosoulful

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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22486876 - 11/06/15 03:59 PM (8 years, 2 months ago)

Why inject threw the polyfill at all? Just open up the plate in your SAB and drop some spore solution in the center of the dish with a flame-sterilized needle. You only need a single drop, too much will just flood the plate, and growth will be delayed until it all evaporates.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Psilosoulful]
    #22486889 - 11/06/15 04:01 PM (8 years, 2 months ago)

Ah, well, my cat damaged the SAB earlier this morning, and I haven't gotten around to fixing it yet. Even if I do fix it, I have to modify it so I have a slot on the side that can be opened to allow larger agar dishes in.



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OfflineMachiavelliavore
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22486916 - 11/06/15 04:09 PM (8 years, 2 months ago)

Yes it will dirty it a bit, but I think you have a pretty good chance of success anyway.  You have an agar dish so big it won't fit through your arm hole?

Please explain this cat incident further.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Machiavelliavore]
    #22486945 - 11/06/15 04:13 PM (8 years, 2 months ago)

• Cats love boxes
• My SAB is a box
• Cat jumped on the box
• Clawed up the plastic wrap
• Chewed on the duct tape

Yeah, it was the only container that I could find that I wasn't going to get yelled at over modifying that was also heat resistant, so I did what I had to. Should give the mycelium plenty of room to develop.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22486960 - 11/06/15 04:16 PM (8 years, 2 months ago)

I think I'm going to modify one of those transparent square bags that the mattress came in and attempt to use that as a still air bag. :stoned:

Still doesn't answer the main question though. I guess it's going to be trial and error type situation.


Edited by YaMoonSun (11/06/15 04:17 PM)


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22487057 - 11/06/15 04:40 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
If the polyfill has been exposed to open air because I foolishly removed the foil a bit early, is it just going to dirty the sterile needle as it passes through the polyfill and ruin my agar plate?



There's a pretty good chance that it COULD Fuck up your plate...this round I would just open the lid and drop spores in...if you are unsure about it, why risk it? And if your needle is too hot when you stick it into your poly, it could potentially melt a small cylinder right through it that could compromise its integrity  as a filter.


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Edited by LocN9ne (11/06/15 04:42 PM)


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OfflinePsilosoulful

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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22487080 - 11/06/15 04:44 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
• Cats love boxes
• My SAB is a box
• Cat jumped on the box
• Clawed up the plastic wrap
• Chewed on the duct tape

Yeah, it was the only container that I could find that I wasn't going to get yelled at over modifying that was also heat resistant, so I did what I had to. Should give the mycelium plenty of room to develop.



Can we see a pic of this SAB :lol:


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: LocN9ne]
    #22487114 - 11/06/15 04:52 PM (8 years, 2 months ago)

Well, took late I suppose; Am hoping for the best. Just applied a single drop to the agar.


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OfflinePsilosoulful

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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22487297 - 11/06/15 05:32 PM (8 years, 2 months ago)

:super:


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Psilosoulful]
    #22487354 - 11/06/15 05:42 PM (8 years, 2 months ago)

There does appear to be a bit of humidity in the container however, is that going to be a problem?



Edited by YaMoonSun (11/06/15 05:43 PM)


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Re: Inoculation through polyfill [Re: Psilosoulful]
    #22487358 - 11/06/15 05:43 PM (8 years, 2 months ago)

Lol I dunno what people are talking about, the polyfil that was exposed was on a lid right?

People inoc through polyfil on grain jars all the time

edit: a little bit of condensation is fine.

edit edit: that seems like a lot of condensation. why are you making your plates so big?


--------------------

                                                                      THROW AWAY YOUR SGFC


Edited by newrook (11/06/15 05:44 PM)


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: newrook]
    #22487377 - 11/06/15 05:45 PM (8 years, 2 months ago)

In a SAB I would assume, with LC nevertheless.


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22487392 - 11/06/15 05:46 PM (8 years, 2 months ago)

How is it any different than someone knocking up a brf cake in open air :shrug:


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22487415 - 11/06/15 05:50 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
• Cats love boxes
• My SAB is a box
• Cat jumped on the box
• Clawed up the plastic wrap
• Chewed on the duct tape





  • Cats need dicipline
  • boxes are cheap, get a new one
  • mist the cat in the face with water when it jumps  on your stuff
  • give it some catnip to keep it busy



--------------------

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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: newrook]
    #22487435 - 11/06/15 05:52 PM (8 years, 2 months ago)

Open air has floating contaminates that can land on your syringe or polyfill prior/during inoculation. Dunno how the hell I'm going to get this water out without a SAB and sterile paper towels or something.


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22487447 - 11/06/15 05:54 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
There does appear to be a bit of humidity in the container however, is that going to be a problem?





nope no problem


--------------------

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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: cronicr]
    #22487448 - 11/06/15 05:54 PM (8 years, 2 months ago)

Bastard needs/wants to go outside, but his "mother" won't let him. Women are fucking psycho.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: cronicr]
    #22487455 - 11/06/15 05:55 PM (8 years, 2 months ago)

Quote:

cronicr said:
Quote:

YaMoonSun said:
There does appear to be a bit of humidity in the container however, is that going to be a problem?





nope no problem



:awegroove: :cheers:
Thanks; Wish me luck :super:


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22487462 - 11/06/15 05:55 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
Bastard needs/wants to go outside, but his "mother" won't let him. Women are fucking psycho.



:awkwardconfusion:


--------------------

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I'm tired do me a favor


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: newrook]
    #22487472 - 11/06/15 05:56 PM (8 years, 2 months ago)

Quote:

why are you making your plates so big?



Was the only kitchenware that was heat resistant that I knew I could modify without hearing someone bitching later :super:

My petris came in the mail a day early though, so I may attempt to pour a few when I establish a new SAB.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: cronicr]
    #22487484 - 11/06/15 05:58 PM (8 years, 2 months ago)

Quote:

cronicr said:
Quote:

YaMoonSun said:
Bastard needs/wants to go outside, but his "mother" won't let him. Women are fucking psycho.



:awkwardconfusion:



It's a relative's cat, and she babies it and refuses to let it go outside. Her last kitty used to all the time, until some dogs that were walking down the street one day practically tore the poor fella in two. She's been really touchy about her pets ever since.


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22487505 - 11/06/15 06:02 PM (8 years, 2 months ago)

ok good i was going to give you a hotline number


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I'm tired do me a favor


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: cronicr]
    #22487527 - 11/06/15 06:07 PM (8 years, 2 months ago)

:lol:


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Re: Inoculation through polyfill [Re: newrook]
    #22487623 - 11/06/15 06:24 PM (8 years, 2 months ago)

Quote:

newrook said:
How is it any different than someone knocking up a brf cake in open air :shrug:




I personally value the time put in and appreciate the work I am doing.

Why take the chance to inoculate anything in open air? Or do any "clean" work?....

Just can't see adding more of a chance to contaminate and mess up a  grow.  :shrug:

If one stays involved with the hobby for any length of time they will soon realize that taking shortcuts, being impatient or generally lazy in this hobby doesn't work out so well the majority of the time.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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OfflineMachiavelliavore
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Re: Inoculation through polyfill [Re: FreeWorldOrder]
    #22487701 - 11/06/15 06:39 PM (8 years, 2 months ago)

The problem is that the poly was sitting out in open air.  If it was covered in foil, the spore load on it would be extremely low to non-existant.  Since the foil was taken off, spores/bacteria having been falling on it for some time.

You can't really swab poly with alcohol and you can't put a red hot needle through it like you can with a SHIP.

That's the problem.  Even so, I expect whatever lodges on the side of the needle as it goes through probably won't be an issue, only something cought in the tip and washed into the plate by the liquid.  It doesn't sound that likely to me though.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Inoculation through polyfill [Re: FreeWorldOrder]
    #22488061 - 11/06/15 08:11 PM (8 years, 2 months ago)

Quote:

FreeWorldOrder said:
Quote:

newrook said:
How is it any different than someone knocking up a brf cake in open air :shrug:




I personally value the time put in and appreciate the work I am doing.

Why take the chance to inoculate anything in open air? Or do any "clean" work?....

Just can't see adding more of a chance to contaminate and mess up a  grow.  :shrug:

If one stays involved with the hobby for any length of time they will soon realize that taking shortcuts, being impatient or generally lazy in this hobby doesn't work out so well the majority of the time.





:customgrow420:

Well... no shit. He was asking if he could do it without a SAB or not


--------------------

                                                                      THROW AWAY YOUR SGFC


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: newrook]
    #22491553 - 11/07/15 03:56 PM (8 years, 2 months ago)

Is it normal for spores to show signs of life on agar in under 24 hours?


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22491614 - 11/07/15 04:10 PM (8 years, 2 months ago)

Can be


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: cronicr]
    #22491746 - 11/07/15 04:36 PM (8 years, 2 months ago)



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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22492111 - 11/07/15 05:37 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
Is it normal for spores to show signs of life on agar in under 24 hours?




Depends on species. That mold you have there can certainly germinate in a day.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Pastywhyte]
    #22492143 - 11/07/15 05:43 PM (8 years, 2 months ago)

That fucking black mold in the ceiling? It's too pretty to be that.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22495568 - 11/08/15 12:53 PM (8 years, 2 months ago)

Yeah, never mind; It looks like a little drop of bacteria.
Hopefully some mycelium escapes so I can get a clean culture for once.


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22495588 - 11/08/15 01:00 PM (8 years, 2 months ago)

That myc is far too disorganized to be just cubes.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Pastywhyte]
    #22495603 - 11/08/15 01:06 PM (8 years, 2 months ago)

How do you mean?

:lolwut:


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22495614 - 11/08/15 01:09 PM (8 years, 2 months ago)

It's a tangled disorganized growth pattern. That's usually a growth pattern mold takes. Cubes grow linear. Your pics are terrible so it's hard to see exactly what's happening but personally I would not even transfer something that looked like that.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Pastywhyte]
    #22495627 - 11/08/15 01:12 PM (8 years, 2 months ago)

Only that gross moist dot is spores/bacteria, the rest is polyfill; Sorry for the shitty picture. It's a weird angle to try to capture.


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Re: Inoculation through polyfill [Re: Pastywhyte]
    #22495631 - 11/08/15 01:13 PM (8 years, 2 months ago)

Quote:

Pastywhyte said:
It's a tangled disorganized growth pattern. That's usually a growth pattern mold takes. Cubes grow linear. Your pics are terrible so it's hard to see exactly what's happening but personally I would not even transfer something that looked like that.




Are you looking at the polyfil in the lid?

the small drop looks like bacteria to me.

edit: Are you taking pics from underneath the plate Yamoon?


--------------------

                                                                      THROW AWAY YOUR SGFC


Edited by newrook (11/08/15 01:14 PM)


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22495635 - 11/08/15 01:14 PM (8 years, 2 months ago)

Lol that's hilarious :facepalm: even with the thread title I didn't get that it was poly :picard: :lol:

With the spores swimming in bacteria they will be damn hard to clean up even if they manage to germ. Start over.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Pastywhyte]
    #22495648 - 11/08/15 01:19 PM (8 years, 2 months ago)

Should I apply a drop of spores to a q-tip and then brush that to a clean agar tray? I mean, if the bacteria came from the spore syringe, will it make a huge difference? I should have gotten anti-bacterial agar.. they don't have it on eBay though :facepalm3:


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22495652 - 11/08/15 01:21 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
Should I apply a drop of spores to a q-tip and then brush that to a clean agar tray? I mean, if the bacteria came from the spore syringe, will it make a huge difference? I should have gotten anti-bacterial agar.. they don't have it on eBay though :facepalm3:





Make proper containers and do more than one plate.

I had to double take it when I first looked too pasty lol


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: newrook]
    #22495659 - 11/08/15 01:22 PM (8 years, 2 months ago)

Got a stack of petri dishes, but I'm waiting. I need to buy/make a new SAB :facepalm3:


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22506791 - 11/10/15 07:53 PM (8 years, 2 months ago)


:girlofdisapproval:


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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22506874 - 11/10/15 08:10 PM (8 years, 2 months ago)

:canthelpbutlaugh:


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Re: Inoculation through polyfill [Re: Psilosoulful]
    #22506896 - 11/10/15 08:13 PM (8 years, 2 months ago)

I swear man I can't tell what I'm looking at in any of your pic :shrug:


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Re: Inoculation through polyfill [Re: Pastywhyte]
    #22507035 - 11/10/15 08:48 PM (8 years, 2 months ago)

my thoughts precisely! what is that??


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InvisibleLocN9ne
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Re: Inoculation through polyfill [Re: spacechildo]
    #22507042 - 11/10/15 08:50 PM (8 years, 2 months ago)

It's a sideways pasty plate that got inoculated through the poly, and bacteria at the inoculation point... I do believe...nope, I just checked again...I have no idea what is going on there.


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Edited by LocN9ne (11/10/15 08:51 PM)


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: LocN9ne]
    #22507056 - 11/10/15 08:55 PM (8 years, 2 months ago)

You nailed it actually :lol:


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Offlinespacechildo
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Re: Inoculation through polyfill [Re: spacechildo]
    #22507084 - 11/10/15 09:02 PM (8 years, 2 months ago)

where's the agar? whats the bacteria growing on? how...but...:stars:


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InvisibleLocN9ne
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Re: Inoculation through polyfill [Re: spacechildo]
    #22507103 - 11/10/15 09:06 PM (8 years, 2 months ago)

The agar is red...that is a pic from the bottom...I think.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: LocN9ne]
    #22507123 - 11/10/15 09:11 PM (8 years, 2 months ago)

The agar is light brown, the lid is red.
The pic is from the bottom, yes.


Edited by YaMoonSun (11/10/15 09:11 PM)


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InvisibleMad Season
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22507592 - 11/10/15 11:39 PM (8 years, 2 months ago)

Bacteria and myc IMO. I can see the carpetty rhizmorphs. Stay away from the yellow bacteria. I don't see why you can't get a clean culture after a few transfers from that plate.


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Mad Season]
    #22507598 - 11/10/15 11:43 PM (8 years, 2 months ago)

I've got my fingers crossed. Need to get find my scalpel and get another agar tray made up. I've got petris, but I haven't reasoned how to get them into the current SAB; Needs to be redesigned.


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InvisibleMad Season
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22507603 - 11/10/15 11:46 PM (8 years, 2 months ago)

Sometimes you just gotta go and upgrade your sab haha. And tbh I didn't realize it was just poly.. Unless the myc is hiding under the poly.. It doesn't look good to me :frown:


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InvisibleLocN9ne
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Re: Inoculation through polyfill [Re: Mad Season]
    #22507610 - 11/10/15 11:50 PM (8 years, 2 months ago)

Quote:

Mad Season said:
Sometimes you just gotta go and upgrade your sab haha. And tbh I didn't realize it was just poly.. Unless the myc is hiding under the poly.. It doesn't look good to me :frown:



Lmmfao. Log out mad...your too high.


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Edited by LocN9ne (11/10/15 11:51 PM)


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: LocN9ne]
    #22507615 - 11/10/15 11:53 PM (8 years, 2 months ago)

:lol:

Yeah; I agree, it looks like shit; Hence the girl of disapproval.


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InvisibleMad Season
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Re: Inoculation through polyfill [Re: LocN9ne]
    #22507625 - 11/11/15 12:02 AM (8 years, 2 months ago)

But I have a speech prepared! Slippery nipples and moist pussy. Damn now I want sex. Time to wake up my gf. Advantages of being taken!

Disadvantage is how much I got bitched at today for not being ocd on my shit. Poo and oats are messy! I need to gtfo of the kitchen. This hobby is too messy for the kitchen! I'm jealous of az.. I want a mycotrailer


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: Mad Season]
    #22507639 - 11/11/15 12:10 AM (8 years, 2 months ago)

Yeah, I'd be pissed, lol


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22513410 - 11/12/15 10:52 AM (8 years, 2 months ago)

I've got half a mind to just open one of the jars I'm trying to colonize and take a tissue sample from the live mycelium and transfer that to agar. The bacteria droplet doesn't seem too promising.


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InvisibleLocN9ne
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22513575 - 11/12/15 11:41 AM (8 years, 2 months ago)

Just wait until the jar is 100% then execute that plan...


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: LocN9ne]
    #22513599 - 11/12/15 11:47 AM (8 years, 2 months ago)

If that's the case I might as well wait until I have identifiable phenos; I just hate waiting :facepalm3:


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InvisibleLocN9ne
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Re: Inoculation through polyfill [Re: YaMoonSun]
    #22513620 - 11/12/15 11:54 AM (8 years, 2 months ago)

You'll be alright...waiting never killed anyone...except military veterans waiting on healthcare


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OfflineYaMoonSun
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Re: Inoculation through polyfill [Re: LocN9ne]
    #22513624 - 11/12/15 11:56 AM (8 years, 2 months ago)

Doesn't help that they keep the temperature in the house at 64F
:justkillmenow:


Edited by YaMoonSun (11/12/15 11:56 AM)


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