Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinenewrook
Sucks at bulk
Male User Gallery


Registered: 03/20/15
Posts: 657
Last seen: 3 months, 5 days
Re: Inoculation through polyfill [Re: YaMoonSun]
    #22495652 - 11/08/15 01:21 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
Should I apply a drop of spores to a q-tip and then brush that to a clean agar tray? I mean, if the bacteria came from the spore syringe, will it make a huge difference? I should have gotten anti-bacterial agar.. they don't have it on eBay though :facepalm3:





Make proper containers and do more than one plate.

I had to double take it when I first looked too pasty lol


--------------------

                                                                      THROW AWAY YOUR SGFC


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Inoculation through polyfill [Re: newrook]
    #22495659 - 11/08/15 01:22 PM (8 years, 2 months ago)

Got a stack of petri dishes, but I'm waiting. I need to buy/make a new SAB :facepalm3:


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Inoculation through polyfill [Re: YaMoonSun]
    #22506791 - 11/10/15 07:53 PM (8 years, 2 months ago)


:girlofdisapproval:


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Inoculation through polyfill [Re: YaMoonSun]
    #22506874 - 11/10/15 08:10 PM (8 years, 2 months ago)

:canthelpbutlaugh:


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Inoculation through polyfill [Re: Psilosoulful]
    #22506896 - 11/10/15 08:13 PM (8 years, 2 months ago)

I swear man I can't tell what I'm looking at in any of your pic :shrug:


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: Inoculation through polyfill [Re: Pastywhyte]
    #22507035 - 11/10/15 08:48 PM (8 years, 2 months ago)

my thoughts precisely! what is that??


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation through polyfill [Re: spacechildo]
    #22507042 - 11/10/15 08:50 PM (8 years, 2 months ago)

It's a sideways pasty plate that got inoculated through the poly, and bacteria at the inoculation point... I do believe...nope, I just checked again...I have no idea what is going on there.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Edited by LocN9ne (11/10/15 08:51 PM)


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Inoculation through polyfill [Re: LocN9ne]
    #22507056 - 11/10/15 08:55 PM (8 years, 2 months ago)

You nailed it actually :lol:


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: Inoculation through polyfill [Re: spacechildo]
    #22507084 - 11/10/15 09:02 PM (8 years, 2 months ago)

where's the agar? whats the bacteria growing on? how...but...:stars:


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation through polyfill [Re: spacechildo]
    #22507103 - 11/10/15 09:06 PM (8 years, 2 months ago)

The agar is red...that is a pic from the bottom...I think.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Inoculation through polyfill [Re: LocN9ne]
    #22507123 - 11/10/15 09:11 PM (8 years, 2 months ago)

The agar is light brown, the lid is red.
The pic is from the bottom, yes.


Edited by YaMoonSun (11/10/15 09:11 PM)


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Inoculation through polyfill [Re: YaMoonSun]
    #22507592 - 11/10/15 11:39 PM (8 years, 2 months ago)

Bacteria and myc IMO. I can see the carpetty rhizmorphs. Stay away from the yellow bacteria. I don't see why you can't get a clean culture after a few transfers from that plate.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Inoculation through polyfill [Re: Mad Season]
    #22507598 - 11/10/15 11:43 PM (8 years, 2 months ago)

I've got my fingers crossed. Need to get find my scalpel and get another agar tray made up. I've got petris, but I haven't reasoned how to get them into the current SAB; Needs to be redesigned.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Inoculation through polyfill [Re: YaMoonSun]
    #22507603 - 11/10/15 11:46 PM (8 years, 2 months ago)

Sometimes you just gotta go and upgrade your sab haha. And tbh I didn't realize it was just poly.. Unless the myc is hiding under the poly.. It doesn't look good to me :frown:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation through polyfill [Re: Mad Season]
    #22507610 - 11/10/15 11:50 PM (8 years, 2 months ago)

Quote:

Mad Season said:
Sometimes you just gotta go and upgrade your sab haha. And tbh I didn't realize it was just poly.. Unless the myc is hiding under the poly.. It doesn't look good to me :frown:



Lmmfao. Log out mad...your too high.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Edited by LocN9ne (11/10/15 11:51 PM)


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Inoculation through polyfill [Re: LocN9ne]
    #22507615 - 11/10/15 11:53 PM (8 years, 2 months ago)

:lol:

Yeah; I agree, it looks like shit; Hence the girl of disapproval.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Inoculation through polyfill [Re: LocN9ne]
    #22507625 - 11/11/15 12:02 AM (8 years, 2 months ago)

But I have a speech prepared! Slippery nipples and moist pussy. Damn now I want sex. Time to wake up my gf. Advantages of being taken!

Disadvantage is how much I got bitched at today for not being ocd on my shit. Poo and oats are messy! I need to gtfo of the kitchen. This hobby is too messy for the kitchen! I'm jealous of az.. I want a mycotrailer


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Inoculation through polyfill [Re: Mad Season]
    #22507639 - 11/11/15 12:10 AM (8 years, 2 months ago)

Yeah, I'd be pissed, lol


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Inoculation through polyfill [Re: YaMoonSun]
    #22513410 - 11/12/15 10:52 AM (8 years, 2 months ago)

I've got half a mind to just open one of the jars I'm trying to colonize and take a tissue sample from the live mycelium and transfer that to agar. The bacteria droplet doesn't seem too promising.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation through polyfill [Re: YaMoonSun]
    #22513575 - 11/12/15 11:41 AM (8 years, 2 months ago)

Just wait until the jar is 100% then execute that plan...


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* PC'ing with polyfill filters mtglu 1,585 3 04/14/03 04:26 PM
by Anno
* innoculating birdseed with polyfill lid jeldrid 3,366 14 03/07/02 07:46 AM
by jeldrid
* problems with grain quarts RedHead 3,576 5 04/02/02 07:37 PM
by RedHead
* innoculating polyfill jars Visigoth 2,364 1 01/05/02 10:42 PM
by Workman
* inoculating birdeed needle wont reach sides Northernsoul 1,102 5 01/25/02 01:26 AM
by Northernsoul
* Innoculate Through Polyfil? Anonymous 897 3 02/26/03 02:51 AM
by G a n j a
* polyfill--inoculation & Cotton Ballz mikejwill 914 1 04/13/03 08:38 PM
by fizzacyst
* PCing with polyfill jars DoDo 3,451 15 01/19/03 05:49 AM
by debianlinux

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,411 topic views. 24 members, 289 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.009 seconds on 15 queries.