Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinedonkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
LAST 2 GROWS FAILED?! This could be why!
    #22486016 - 11/06/15 12:45 PM (8 years, 2 months ago)

Quick backstory: Last 3 grows, first was successful, last 2 failed. Quick summary of what my procedure was with each.

1: Spawned 2 qts. to coir substrate in small tub. Colonized till white. Cased with coir and fruiting by a window without fruiting chamber, as to this tek: http://www.shroomery.org/forums/showflat.php/Number/21288129
Once I saw a small mushroom, I put the tray into a monotub with polyfill, and got amazing results.



2: Did a classic monotub. Waited to fully colonized, replaced tape w/ poly and waited eagerly for some pins. As I was under the assumption that a fully colonized substrate was more or less resistant to contamination, I opened up the lid and took a close look at the substrate 1 or 2 times. Green mold quickly resulted. FAIL! back to drawing board



3: This past grow was done with a fresh syringe, same techniques but I chalked up my past failed grow to a bad syringe as I was under the aforementioned assumption. Colonized perfectly, replaced tape w/ poly and peeked at substrate a few times. Green mold again resulted. FAIL! quit growing for a few months, as I was very confused and discouraged at this point.

My new theory: The question baffled me for months. How could an open-air grow turn out so great, when two in an enclosed mono tub con tamed with just a few peeks? The most likely explanation: the open air grow got just as many contam spores as the 2 monos, but they weren't able to germinate because of the drier room air, so the mycelium prospered. The two monos got contaminated because of the naturally higher ambient humidity, the contams had an edge. This next grow I will be either doing another open-air grow, or not disturb my monos anymore :cool: I am optimistic for the future.


Extras: Filter Print Post Top
Offlinedryhub
Stranger

Registered: 10/20/15
Posts: 24
Last seen: 7 years, 10 months
Re: LAST 2 GROWS FAILED?! This could be why! [Re: donkeycong] * 1
    #22486098 - 11/06/15 01:14 PM (8 years, 2 months ago)

i dont have any idea but you should have take some clones of those in the first tub


Extras: Filter Print Post Top
Offlinewolf8312
Pennywise
 User Gallery

Registered: 10/01/12
Posts: 2,356
Last seen: 4 days, 3 hours
Re: LAST 2 GROWS FAILED?! This could be why! [Re: donkeycong]
    #22486103 - 11/06/15 01:15 PM (8 years, 2 months ago)

Quote:

donkeycong said:
Quick backstory: Last 3 grows, first was successful, last 2 failed. Quick summary of what my procedure was with each.

1: Spawned 2 qts. to coir substrate in small tub. Colonized till white. Cased with coir and fruiting by a window without fruiting chamber, as to this tek: http://www.shroomery.org/forums/showflat.php/Number/21288129
Once I saw a small mushroom, I put the tray into a monotub with polyfill, and got amazing results.



2: Did a classic monotub. Waited to fully colonized, replaced tape w/ poly and waited eagerly for some pins. As I was under the assumption that a fully colonized substrate was more or less resistant to contamination, I opened up the lid and took a close look at the substrate 1 or 2 times. Green mold quickly resulted. FAIL! back to drawing board



3: This past grow was done with a fresh syringe, same techniques but I chalked up my past failed grow to a bad syringe as I was under the aforementioned assumption. Colonized perfectly, replaced tape w/ poly and peeked at substrate a few times. Green mold again resulted. FAIL! quit growing for a few months, as I was very confused and discouraged at this point.

My new theory: The question baffled me for months. How could an open-air grow turn out so great, when two in an enclosed mono tub con tamed with just a few peeks? The most likely explanation: the open air grow got just as many contam spores as the 2 monos, but they weren't able to germinate because of the drier room air, so the mycelium prospered. The two monos got contaminated because of the naturally higher ambient humidity, the contams had an edge. This next grow I will be either doing another open-air grow, or not disturb my monos anymore :cool: I am optimistic for the future.




My first 2 tubs failed too it was demoralizing but after that I found it rather hard not to be successful.

One thing you didnt do an 'open air grow' and all your grows were just as exposed to mold spores as the other.

The conditions in a mono tub are more likely to produce green mold eventually but if your spawn is OK it will have long since colonized and outrun any potential contaminants. It goes green that quickly because it was already there. 

I think you are probably over thinking it. First suspect is your syringe, then check your jars, lids, your PC maybe (pressure gage), and then review your sterile technique. What is your sterile technique by the way?

Most likely culprit when grows like yours fail its due to poor sterile technique (bad syringe, cross contamination) and not the spawn run or pasteurization especially when dealing with coir. It could though be sometimes that you didnt cook the coir properly and the spawn didnt colonize quickly enough as a result.

I never got into agar cause my prints were always very reliable but had I continued to fail I think I would have had to as its really the only way to know for sure what the problem is.

Work from the assumption that its your spawn thats dirty though, as thats nearly always the case in these matters.

And that first tub is multispore? Surprised you even need to grow again!


--------------------
"I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of."

Pennywise the dancing clown



Edited by wolf8312 (11/06/15 01:56 PM)


Extras: Filter Print Post Top
Offlinetorpy
Stranger

Registered: 09/09/15
Posts: 38
Last seen: 7 years, 11 months
Re: LAST 2 GROWS FAILED?! This could be why! [Re: dryhub]
    #22486125 - 11/06/15 01:21 PM (8 years, 2 months ago)

^^ seriously! I need a print of that


Extras: Filter Print Post Top
Offlinedonkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
Re: LAST 2 GROWS FAILED?! This could be why! [Re: donkeycong]
    #22486215 - 11/06/15 01:44 PM (8 years, 2 months ago)

Haha I got lucky w/ the multi-spore. It blessed me with some kick-ass chocolates


Edited by donkeycong (11/06/15 01:47 PM)


Extras: Filter Print Post Top
Offlinewolf8312
Pennywise
 User Gallery

Registered: 10/01/12
Posts: 2,356
Last seen: 4 days, 3 hours
Re: LAST 2 GROWS FAILED?! This could be why! [Re: donkeycong]
    #22486283 - 11/06/15 01:59 PM (8 years, 2 months ago)

That tub is fricking amazing to be honest how were the chocolates?


--------------------
"I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of."

Pennywise the dancing clown



Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: LAST 2 GROWS FAILED?! This could be why! [Re: wolf8312]
    #22486296 - 11/06/15 02:02 PM (8 years, 2 months ago)

Your grow was contaminated before you spawned it, dirty spawn was 95% likely the cause, sometimes we miss shit and it happens to EVERYBODY


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinedonkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
Re: LAST 2 GROWS FAILED?! This could be why! [Re: wolf8312]
    #22486305 - 11/06/15 02:06 PM (8 years, 2 months ago)

Really strong. I put 1.7 in each, took 4 the first time and I went a lil too far down the rabbit hole :mushroom2:


Extras: Filter Print Post Top
Offlinedonkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
Re: LAST 2 GROWS FAILED?! This could be why! [Re: cronicr]
    #22486313 - 11/06/15 02:08 PM (8 years, 2 months ago)

This could easily be the case as well. The first failed tub was spawned with G2G, so at first I thought this was the case, but the 3rd was with a fresh syringe so that threw me off a bit


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: LAST 2 GROWS FAILED?! This could be why! [Re: donkeycong]
    #22486323 - 11/06/15 02:10 PM (8 years, 2 months ago)

syringes fail sometimes, they are very rarely 100% clean


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: LAST 2 GROWS FAILED?! This could be why! [Re: cronicr]
    #22486372 - 11/06/15 02:19 PM (8 years, 2 months ago)

Green mold like trich will hide in your spawn. You will rarely see a green jar. It won't show after G2G or even spawning.

It will show up after you fruit your tub. You're essentially fruiting the mold too.

I lose tubs to green mold all the time from G2G.

Try innoculating with clean cultures on agar


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlinewolf8312
Pennywise
 User Gallery

Registered: 10/01/12
Posts: 2,356
Last seen: 4 days, 3 hours
Re: LAST 2 GROWS FAILED?! This could be why! [Re: mushpunx]
    #22486532 - 11/06/15 02:52 PM (8 years, 2 months ago)

Quote:

mushpunx said:
Green mold like trich will hide in your spawn. You will rarely see a green jar. It won't show after G2G or even spawning.

It will show up after you fruit your tub. You're essentially fruiting the mold too.

I lose tubs to green mold all the time from G2G.






Does that happen even with your flow hood mushpunx?


--------------------
"I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of."

Pennywise the dancing clown



Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: LAST 2 GROWS FAILED?! This could be why! [Re: wolf8312]
    #22487996 - 11/06/15 07:54 PM (8 years, 2 months ago)

Quote:

wolf8312 said:
Quote:

mushpunx said:
Green mold like trich will hide in your spawn. You will rarely see a green jar. It won't show after G2G or even spawning.

It will show up after you fruit your tub. You're essentially fruiting the mold too.

I lose tubs to green mold all the time from G2G.






Does that happen even with your flow hood mushpunx?




Oh of course dude haha flow hoods are awesome but honestly you have to be more on your game sterile tek wise than in a still air box


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineMachiavelliavore
Vermiculite Hater
 User Gallery


Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
Re: LAST 2 GROWS FAILED?! This could be why! [Re: mushpunx]
    #22488265 - 11/06/15 08:56 PM (8 years, 2 months ago)

:whathesaid::whathesaid::whathesaid::whathesaid::whathesaid::whathesaid::whathesaid::whathesaid:

Bad inoculant.  The humidity of the mono may show the mold already there a little faster or promote the death by mold of a spent substrate faster, but it won't cause contamination.

If you want reliable results, use agar or at least make your spore syringes from invitro grows in a still air box with excellent sterile technique.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Extras: Filter Print Post Top
Offlinewolf8312
Pennywise
 User Gallery

Registered: 10/01/12
Posts: 2,356
Last seen: 4 days, 3 hours
Re: LAST 2 GROWS FAILED?! This could be why! [Re: mushpunx]
    #22488507 - 11/06/15 09:46 PM (8 years, 2 months ago)

Quote:

mushpunx said:
Quote:

wolf8312 said:
Quote:

mushpunx said:
Green mold like trich will hide in your spawn. You will rarely see a green jar. It won't show after G2G or even spawning.

It will show up after you fruit your tub. You're essentially fruiting the mold too.

I lose tubs to green mold all the time from G2G.






Does that happen even with your flow hood mushpunx?




Oh of course dude haha flow hoods are awesome but honestly you have to be more on your game sterile tek wise than in a still air box




Wow thats a reality check!

I always thought of flow hoods as being fool proof like me sitting there with open jars farting and sneezing around the place with no problems!


--------------------
"I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of."

Pennywise the dancing clown



Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: LAST 2 GROWS FAILED?! This could be why! [Re: wolf8312]
    #22489076 - 11/07/15 01:55 AM (8 years, 2 months ago)

Haha no. The air coming out of the HEPA is sterile, and it is in laminar flow which means any contams will get caught in the flow and blow straight out, without settling down any further than they are. So they won't land in an open sterile container.

But the air *is* still moving, so if say you move your glove between the filter and say an open agar plate it can blow contams right off your glove and into the plate.

So you have to be extra careful with your hand movements and your prep work, shower, face mask , gloves, everything wiped off with ISO before it goes in front of the hood.
No different than a still air box , you have to do that for both.

But the benifits are great, you get to work right out in the open, they're fantastic for spawn bags and uou don't have to look thru glass at your work.


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineMachiavelliavore
Vermiculite Hater
 User Gallery


Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
Re: LAST 2 GROWS FAILED?! This could be why! [Re: mushpunx]
    #22489079 - 11/07/15 01:59 AM (8 years, 2 months ago)

Huh, so you take your SAB technique and then work like gravity is horizontal coming toward you.  Interesting.

Are the upward FH's easier to use?  I am assuming upward makes a lot more sense that downward.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: LAST 2 GROWS FAILED?! This could be why! [Re: Machiavelliavore]
    #22489229 - 11/07/15 04:36 AM (8 years, 2 months ago)

Quote:

Machiavelliavore said:
Huh, so you take your SAB technique and then work like gravity is horizontal coming toward you.  Interesting.

Are the upward FH's easier to use?  I am assuming upward makes a lot more sense that downward.





I dunno what an upward F H is but I do know the overhead ones, I don't think they are very well suited for mycology.

  But yea man you're right. The only difference is air is blowing towards you so that needs to be accounted for in your motions.

Both are excellent for this hobby. I think that after learning sterile tek and getting good in SAB that if you decide you really enjoy this hobby and are going to be doing it a lot then you should definitely build a hood. ThEyre actually pretty affordable if you do the building.

The two best things about them are spawn bags and agar work. Pouring plates becomes much less of a chore like it is even in tall still air boxes and you can see your plates clearly and up close while ur working. And spawn bags are pretty cumbersome again een in vèry large SAB. And you can inflate the bags for a  good plenum of air which you cannot do in SAB. M

Some people will tell you thry are overkill for home cultivation. But I think theyre awesome


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Why grow on agar? stcore 2,012 1 07/29/02 05:03 PM
by bluhoney
* Potency vs Substrate SeussA 5,789 10 05/21/02 02:05 PM
by Baby_Hitler
* dry substrate? zandorf 1,583 8 09/07/02 09:10 AM
by zandorf
* Substrates... other than straw celsius 745 3 09/28/01 05:46 PM
by celsius
* Bulk Substrate Growing Questions kid_alamo 2,409 2 04/01/02 11:57 AM
by XAZIA
* Shroom Growing Outside? TheJoker 3,873 17 06/16/01 11:50 PM
by Gilgamesh
* First Time Problems - Growing from Sides Only?! level123 500 5 03/03/18 09:03 AM
by grow4fun
* An introduction and Mazapatec mono grow log
( 1 2 3 4 all )
grow4fun 6,104 73 04/21/18 08:54 AM
by grow4fun

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,360 topic views. 45 members, 364 guests and 58 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.007 seconds on 12 queries.