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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 8 hours
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Quote:
blindingleaf said:
Quote:
Not using a SAB is like fucking a hooker without a condom.
then would an open air inoc port be like a dental dam?
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 Amateur Mycologists United AMU Q&A
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 8 hours
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Re: Are Newbs Not Using SABs? [Re: mushpunx]
#22486324 - 11/06/15 02:11 PM (8 years, 2 months ago) |
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Honestly I have never used a still air box to inoculate a PF jar. And Ive never had a PF jar contam.
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 Amateur Mycologists United AMU Q&A
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Are Newbs Not Using SABs? [Re: mushpunx]
#22486329 - 11/06/15 02:12 PM (8 years, 2 months ago) |
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i did years w/o a sab, ships and dry layers, had my fair share of contams and pulled some sweet grows though
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Are Newbs Not Using SABs? [Re: YaMoonSun]
#22486715 - 11/06/15 03:27 PM (8 years, 2 months ago) |
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Quote:
YaMoonSun said: Amen, lol; With a little LED light so you can see

Niceee.
I wonder if the micro heat generated by the led lighting could create micro air disturbances in your design, ending up for you with a higher contamination rate than without the lights.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: Are Newbs Not Using SABs? [Re: Mr. Alien]
#22486748 - 11/06/15 03:32 PM (8 years, 2 months ago) |
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I was thinking about that, which is why I'm using a controllable T6 on the lowest setting.
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: Are Newbs Not Using SABs? [Re: Mr. Alien]
#22486749 - 11/06/15 03:32 PM (8 years, 2 months ago) |
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Alien!!
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Quote:
YaMoonSun said: I was thinking about that, which is why I'm using a controllable T6 on the lowest setting. 
Yeah but even with the lowest setting there is always a tiny little bit of heat? Why just don't just put the lights outside, like make a special window and place the light outside the box pointing to that window?
Quote:
PsilocyBen17 said:
Alien!!
hiiiii
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: Are Newbs Not Using SABs? [Re: Mr. Alien]
#22486827 - 11/06/15 03:47 PM (8 years, 2 months ago) |
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I suppose, but there's still going to be heat transfer unless I elevate the light just a tad above the new lighting window modification. It's not like I don't have to modify the box anyways. Might as well, lol
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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Re: Are Newbs Not Using SABs? [Re: YaMoonSun]
#22486848 - 11/06/15 03:52 PM (8 years, 2 months ago) |
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I cover the top with plastic wrap and tape the seams with packaging tape. That lets the overhead light be plenty to see.
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brandonshmandon
Caucasian!



Registered: 07/07/15
Posts: 205
Last seen: 3 years, 8 months
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Re: Are Newbs Not Using SABs? [Re: thebug76]
#22486917 - 11/06/15 04:10 PM (8 years, 2 months ago) |
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I've done nothing but open air noccs since i started. Done well over 50 jars so far and tossed1 to green and 3 due to wetspot it's not rocket science. Meanwhile i know a guy who uses a sab and has had about a %50 contam rate. If your sterile procedures are up to par your fine. Might I add i only inoculate with syringes. And i am making a sab this weekend but i only need it for agar work.
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,310
Last seen: 21 days, 17 hours
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Re: Are Newbs Not Using SABs? [Re: YaMoonSun]
#22487307 - 11/06/15 05:34 PM (8 years, 2 months ago) |
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Once you learn sterile technique and if you are not a complete moron. You can pretty much do mycology inside a cat shit box. Adleast grow cubes.
Edited by Groo (11/06/15 05:34 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Are Newbs Not Using SABs? [Re: YaMoonSun]
#22487496 - 11/06/15 06:00 PM (8 years, 2 months ago) |
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Quote:
YaMoonSun said: I suppose, but there's still going to be heat transfer unless I elevate the light just a tad above the new lighting window modification. It's not like I don't have to modify the box anyways. Might as well, lol
Dude just get a clear tote and put the light outside it. 
Problem solved.
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hostileuniverse
Stranger



Registered: 05/14/15
Posts: 8,602
Loc: 'Merica
Last seen: 6 years, 7 months
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Quote:
PsilocyBen17 said: Not using a SAB is like fucking a hooker without a condom. You might get lucky once, but if you do you shouldn't try it again!
once you got every disease they have to offer, its all good
honestly though, if it weren't for us noobs fucking everything up, how would you spend your evenings? lol
***I personally appreciate all y'alls help!
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Quote:
hostileuniverse said:
Quote:
PsilocyBen17 said: Not using a SAB is like fucking a hooker without a condom. You might get lucky once, but if you do you shouldn't try it again!
once you got every disease they have to offer, its all good
honestly though, if it weren't for us noobs fucking everything up, how would you spend your evenings? lol
***I personally appreciate all y'alls help!
Bullshitting in the jounals...duh.
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Q&A US vs. THEM The more I learn, the less I know.
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hostileuniverse
Stranger



Registered: 05/14/15
Posts: 8,602
Loc: 'Merica
Last seen: 6 years, 7 months
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Re: Are Newbs Not Using SABs? [Re: LocN9ne]
#22487925 - 11/06/15 07:31 PM (8 years, 2 months ago) |
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Quote:
hostileuniverse said:
Quote:
PsilocyBen17 said: Not using a SAB is like fucking a hooker without a condom. You might get lucky once, but if you do you shouldn't try it again!
once you got every disease they have to offer, its all good
honestly though, if it weren't for us noobs fucking everything up, how would you spend your evenings? lol
***I personally appreciate all y'alls help!
Quote:
LocN9ne said:
Quote:
hostileuniverse said:
Quote:
PsilocyBen17 said: Not using a SAB is like fucking a hooker without a condom. You might get lucky once, but if you do you shouldn't try it again!
once you got every disease they have to offer, its all good
honestly though, if it weren't for us noobs fucking everything up, how would you spend your evenings? lol
***I personally appreciate all y'alls help!
Bullshitting in the jounals...duh.
lol,
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Kalistis


Registered: 09/06/15
Posts: 2,265
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I can't imagine doing all the agar work I do without a SAB. I have very low contamination rate when I pour and complete my work in my SAB. In fact, my last 2 contaminated plates were from cloned tissue and I think I used too much.... had nothing to do with introducing contaminant to the plates through the air.
I think you could probably get by without a SAB if you never plan on fully experiencing most aspects of this hobby. If you are content with MS syringes and pf tek or even jars with SHIPs then so be it. They are used for a reason.... if you know someone that has a 50% contamination rate in his SAB, I'd love to know his set-up and technique.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Are Newbs Not Using SABs? [Re: Kalistis]
#22488060 - 11/06/15 08:11 PM (8 years, 2 months ago) |
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Quote:
Kalistis said: I can't imagine doing all the agar work I do without a SAB. I have very low contamination rate when I pour and complete my work in my SAB. In fact, my last 2 contaminated plates were from cloned tissue and I think I used too much.... had nothing to do with introducing contaminant to the plates through the air.
I think you could probably get by without a SAB if you never plan on fully experiencing most aspects of this hobby. If you are content with MS syringes and pf tek or even jars with SHIPs then so be it. They are used for a reason.... if you know someone that has a 50% contamination rate in his SAB, I'd love to know his set-up and technique.
That video that was recently posted of that dude cutting up his cakes with a flame sterilized butcher knife that he cooled down with his breath in open air probably has a 50% success rate...lol
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Q&A US vs. THEM The more I learn, the less I know.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Are Newbs Not Using SABs? [Re: LocN9ne]
#22488087 - 11/06/15 08:17 PM (8 years, 2 months ago) |
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LOL! He actually blew on it. Nice. Why not ejaculate on it? That's gotta be more sterile.
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Re: Are Newbs Not Using SABs? [Re: LocN9ne]
#22488236 - 11/06/15 08:47 PM (8 years, 2 months ago) |
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Quote:
LocN9ne said: That video that was recently posted of that dude cutting up his cakes with a flame sterilized butcher knife that he cooled down with his breath in open air probably has a 50% success rate...lol
He could literally have pissed on it and been better off
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Are Newbs Not Using SABs? [Re: cronicr]
#22488273 - 11/06/15 08:57 PM (8 years, 2 months ago) |
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Quote:
cronicr said: who cares, if noobs started using sabs and following instructions all of us regulars would be having this convo in the pub, let them learn the hard way or we become useless
I didn't use a sab or agar for like 3 years. Betcha guys didn't know that. Wow what a difference the switch made. Never looking back!
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