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OfflinePsilocyBen17
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Registered: 10/20/13
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Are Newbs Not Using SABs?
    #22485891 - 11/06/15 12:14 PM (8 years, 2 months ago)

I took a short hiatus from cultivating but am back now after a few months. I've been active on the forms for 3 days now and have found a TON of people not bothering to use SABs. Whats going on? Where are people getting the idea that open air inoculations are smart? :shrug:

Don't mean to be a downer. Just concerned.

Quote:

Censored Username #1: 

Now, I dont use a SAB.  I shut all vents off in my shower after I take a shower and then wait until the humidity is gone.  Sort of like a still air bathroom.  At first I thought that may be my problem, but all 10/10 jars?!

I am building a SAB now, but I feel like that can't be the cause as all of the brf jars were fine in the bathroom.  WBS could be totally different though.





Quote:

Censored Username #2:
Well I too inoculate in open air even though its one of the worst ways to do it. ( my SAB sucks) but if everything is getting infected its probably something else unless your sterile technique is that bad. Are both spores from the same vendor purchased at the same time? Maybe the print was dirty. If I had a problem like yours I would definitely turn to agar to zone in on the problem. I think that should be your next step. If the agar has spotless growth you know its not the syringes.




Quote:

PsilocyBen17 said:
consider using a SAB.



Quote:

Censored Username #3:
I will have to try that. I live in coastal nc, so mold is a big thing here.




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OfflineRipvanBongBowl420
Clearly Colorful
Registered: 10/26/15
Posts: 162
Last seen: 7 years, 6 months
Re: Are Newbs Not Using SABs? [Re: PsilocyBen17]
    #22485903 - 11/06/15 12:18 PM (8 years, 2 months ago)

I honestly didnt my first grow either Ben.. I'm going to this time and I'm fully aware that I just got realllly lucky my first time :smile:


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OfflinePsilocyBen17
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Registered: 10/20/13
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Re: Are Newbs Not Using SABs? [Re: RipvanBongBowl420]
    #22485908 - 11/06/15 12:20 PM (8 years, 2 months ago)

:thumbup:


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OfflineYaMoonSun
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Registered: 10/23/14
Posts: 3,967
Loc: NY
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Re: Are Newbs Not Using SABs? [Re: PsilocyBen17]
    #22485912 - 11/06/15 12:21 PM (8 years, 2 months ago)

Guilty; I didn't make mine until last week, and I've been here a solid year.


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Offlinemidnightmaraude
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Registered: 05/09/14
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Re: Are Newbs Not Using SABs? [Re: YaMoonSun]
    #22485937 - 11/06/15 12:28 PM (8 years, 2 months ago)

I only use mine for grain to grain or agar.  Otherwise I have ships and sfds on my jars for needle injections and do those outside sab. No issues yet.


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OfflineYaMoonSun
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Registered: 10/23/14
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Re: Are Newbs Not Using SABs? [Re: midnightmaraude]
    #22485955 - 11/06/15 12:33 PM (8 years, 2 months ago)

I'm going to need one for making agar transfers; Going to have to build a new one as mine was just ruined today.


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Offlinethebug76
2 years in.
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Registered: 05/31/15
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Re: Are Newbs Not Using SABs? [Re: midnightmaraude]
    #22485974 - 11/06/15 12:37 PM (8 years, 2 months ago)

I'm a noob myself, but a SAB was the first thing I built. I mean, why not, a cardboard box or plastic tub, plastic wrap, and some tape.


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OfflineHonkyGeorge
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Re: Are Newbs Not Using SABs? [Re: midnightmaraude]
    #22485979 - 11/06/15 12:37 PM (8 years, 2 months ago)

I didn't use a SAB. Tried inoculating in my kitchen; made everything as clean as possible; got bacteria and metabolites. They were rye berry bags, and they were part of a kit...I know better now.


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OfflineYaMoonSun
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Registered: 10/23/14
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Re: Are Newbs Not Using SABs? [Re: thebug76]
    #22485984 - 11/06/15 12:38 PM (8 years, 2 months ago)

Amen, lol; With a little LED light so you can see


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Offlinemidnightmaraude
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Re: Are Newbs Not Using SABs? [Re: HonkyGeorge]
    #22485986 - 11/06/15 12:39 PM (8 years, 2 months ago)

Well the kit could've been the culprit there.


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OfflineHonkyGeorge
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Re: Are Newbs Not Using SABs? [Re: midnightmaraude]
    #22486030 - 11/06/15 12:51 PM (8 years, 2 months ago)

Quote:

midnightmaraude said:
Well the kit could've been the culprit there.



I'm sure the kit had a lot to do with it, but opening the syringe in kitchen air to put on the needle didn't help. Doing that probably ruined all those GT spores.


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InvisibleSrirachi
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Re: Are Newbs Not Using SABs? [Re: PsilocyBen17]
    #22486072 - 11/06/15 01:05 PM (8 years, 2 months ago)

My first SAB was a clear trash bag. Considering how cheap and easy it is, and how much it increases the likelihood of success... I don't get why people try to cut that particular corner.

My current SAB is made of 1/2" HDPE and aluminum box tubing, with LED lighting:



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Offlinemidnightmaraude
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Re: Are Newbs Not Using SABs? [Re: HonkyGeorge]
    #22486081 - 11/06/15 01:09 PM (8 years, 2 months ago)

Quote:

HonkyGeorge said:
Quote:

midnightmaraude said:
Well the kit could've been the culprit there.



I'm sure the kit had a lot to do with it, but opening the syringe in kitchen air to put on the needle didn't help. Doing that probably ruined all those GT spores.




I doubt it.
Or your syringe was contaminated to begin with.


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OfflineHonkyGeorge
Invest in Franquito Industries
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Re: Are Newbs Not Using SABs? [Re: midnightmaraude]
    #22486143 - 11/06/15 01:25 PM (8 years, 2 months ago)

Quote:

midnightmaraude said:
Quote:

HonkyGeorge said:
Quote:

midnightmaraude said:
Well the kit could've been the culprit there.



I'm sure the kit had a lot to do with it, but opening the syringe in kitchen air to put on the needle didn't help. Doing that probably ruined all those GT spores.




I doubt it.
Or your syringe was contaminated to begin with.



Nah. They fruited Ok. There were just a lot of metabolites and growth was painfully slow.


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OfflinePsilocyBen17
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Re: Are Newbs Not Using SABs? [Re: HonkyGeorge]
    #22486154 - 11/06/15 01:29 PM (8 years, 2 months ago)

Not using a SAB is like fucking a hooker without a condom. You might get lucky once, but if you do you shouldn't try it again!


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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
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Re: Are Newbs Not Using SABs? [Re: PsilocyBen17]
    #22486246 - 11/06/15 01:51 PM (8 years, 2 months ago)

Quote:

Not using a SAB is like fucking a hooker without a condom.




then would an open air inoc port be like a dental dam?


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MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
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Re: Are Newbs Not Using SABs? [Re: blindingleaf]
    #22486263 - 11/06/15 01:55 PM (8 years, 2 months ago)

Quote:

blindingleaf said:
Quote:

Not using a SAB is like fucking a hooker without a condom.




then would an open air inoc port be like a dental dam?



Checks out...


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Q&A
US vs. THEM

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OfflinecronicrFacebook
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Re: Are Newbs Not Using SABs? [Re: LocN9ne]
    #22486272 - 11/06/15 01:57 PM (8 years, 2 months ago)

who cares, if noobs started using sabs and following instructions all of us regulars would be having this convo in the pub, let them learn the hard way or we become useless:crazy:


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
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Re: Are Newbs Not Using SABs? [Re: cronicr]
    #22486279 - 11/06/15 01:59 PM (8 years, 2 months ago)

Experience is truly the best teacher


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InvisibleSrirachi
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Re: Are Newbs Not Using SABs? [Re: cronicr]
    #22486289 - 11/06/15 02:01 PM (8 years, 2 months ago)

Did you ever see that contest I ran years ago where I gave people all the crap to do it right if they posted the worst fuckup story? Never did hear how any of those people that won wound up doing.

A few of the people who posted in it went on to do quite well for themselves though by simply not doing anything new until they were good at doing something old.


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