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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Are Newbs Not Using SABs?
#22485891 - 11/06/15 12:14 PM (8 years, 2 months ago) |
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I took a short hiatus from cultivating but am back now after a few months. I've been active on the forms for 3 days now and have found a TON of people not bothering to use SABs. Whats going on? Where are people getting the idea that open air inoculations are smart? 
Don't mean to be a downer. Just concerned.
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Censored Username #1:
Now, I dont use a SAB. I shut all vents off in my shower after I take a shower and then wait until the humidity is gone. Sort of like a still air bathroom. At first I thought that may be my problem, but all 10/10 jars?!
I am building a SAB now, but I feel like that can't be the cause as all of the brf jars were fine in the bathroom. WBS could be totally different though.
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Censored Username #2: Well I too inoculate in open air even though its one of the worst ways to do it. ( my SAB sucks) but if everything is getting infected its probably something else unless your sterile technique is that bad. Are both spores from the same vendor purchased at the same time? Maybe the print was dirty. If I had a problem like yours I would definitely turn to agar to zone in on the problem. I think that should be your next step. If the agar has spotless growth you know its not the syringes.
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PsilocyBen17 said: consider using a SAB.
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Censored Username #3: I will have to try that. I live in coastal nc, so mold is a big thing here.
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RipvanBongBowl420
Clearly Colorful
Registered: 10/26/15
Posts: 162
Last seen: 7 years, 6 months
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I honestly didnt my first grow either Ben.. I'm going to this time and I'm fully aware that I just got realllly lucky my first time
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Guilty; I didn't make mine until last week, and I've been here a solid year.
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 7 years, 1 month
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Re: Are Newbs Not Using SABs? [Re: YaMoonSun]
#22485937 - 11/06/15 12:28 PM (8 years, 2 months ago) |
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I only use mine for grain to grain or agar. Otherwise I have ships and sfds on my jars for needle injections and do those outside sab. No issues yet.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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I'm going to need one for making agar transfers; Going to have to build a new one as mine was just ruined today.
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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I'm a noob myself, but a SAB was the first thing I built. I mean, why not, a cardboard box or plastic tub, plastic wrap, and some tape.
-------------------- Bug
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HonkyGeorge
Invest in Franquito Industries

Registered: 11/02/15
Posts: 33
Last seen: 6 years, 5 months
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I didn't use a SAB. Tried inoculating in my kitchen; made everything as clean as possible; got bacteria and metabolites. They were rye berry bags, and they were part of a kit...I know better now.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: Are Newbs Not Using SABs? [Re: thebug76]
#22485984 - 11/06/15 12:38 PM (8 years, 2 months ago) |
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Amen, lol; With a little LED light so you can see
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 7 years, 1 month
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Re: Are Newbs Not Using SABs? [Re: HonkyGeorge]
#22485986 - 11/06/15 12:39 PM (8 years, 2 months ago) |
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Well the kit could've been the culprit there.
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HonkyGeorge
Invest in Franquito Industries

Registered: 11/02/15
Posts: 33
Last seen: 6 years, 5 months
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Quote:
midnightmaraude said: Well the kit could've been the culprit there.
I'm sure the kit had a lot to do with it, but opening the syringe in kitchen air to put on the needle didn't help. Doing that probably ruined all those GT spores.
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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My first SAB was a clear trash bag. Considering how cheap and easy it is, and how much it increases the likelihood of success... I don't get why people try to cut that particular corner.
My current SAB is made of 1/2" HDPE and aluminum box tubing, with LED lighting:
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 7 years, 1 month
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Re: Are Newbs Not Using SABs? [Re: HonkyGeorge]
#22486081 - 11/06/15 01:09 PM (8 years, 2 months ago) |
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Quote:
HonkyGeorge said:
Quote:
midnightmaraude said: Well the kit could've been the culprit there.
I'm sure the kit had a lot to do with it, but opening the syringe in kitchen air to put on the needle didn't help. Doing that probably ruined all those GT spores.
I doubt it. Or your syringe was contaminated to begin with.
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HonkyGeorge
Invest in Franquito Industries

Registered: 11/02/15
Posts: 33
Last seen: 6 years, 5 months
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Quote:
midnightmaraude said:
Quote:
HonkyGeorge said:
Quote:
midnightmaraude said: Well the kit could've been the culprit there.
I'm sure the kit had a lot to do with it, but opening the syringe in kitchen air to put on the needle didn't help. Doing that probably ruined all those GT spores.
I doubt it. Or your syringe was contaminated to begin with.
Nah. They fruited Ok. There were just a lot of metabolites and growth was painfully slow.
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: Are Newbs Not Using SABs? [Re: HonkyGeorge]
#22486154 - 11/06/15 01:29 PM (8 years, 2 months ago) |
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Not using a SAB is like fucking a hooker without a condom. You might get lucky once, but if you do you shouldn't try it again!
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
Not using a SAB is like fucking a hooker without a condom.
then would an open air inoc port be like a dental dam?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Quote:
blindingleaf said:
Quote:
Not using a SAB is like fucking a hooker without a condom.
then would an open air inoc port be like a dental dam?
Checks out...
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Q&A US vs. THEM The more I learn, the less I know.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Are Newbs Not Using SABs? [Re: LocN9ne]
#22486272 - 11/06/15 01:57 PM (8 years, 2 months ago) |
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who cares, if noobs started using sabs and following instructions all of us regulars would be having this convo in the pub, let them learn the hard way or we become useless
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: Are Newbs Not Using SABs? [Re: cronicr]
#22486279 - 11/06/15 01:59 PM (8 years, 2 months ago) |
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Experience is truly the best teacher
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Re: Are Newbs Not Using SABs? [Re: cronicr]
#22486289 - 11/06/15 02:01 PM (8 years, 2 months ago) |
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Did you ever see that contest I ran years ago where I gave people all the crap to do it right if they posted the worst fuckup story? Never did hear how any of those people that won wound up doing.
A few of the people who posted in it went on to do quite well for themselves though by simply not doing anything new until they were good at doing something old.
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