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Newcap
Stranger
Registered: 11/06/15
Posts: 3
Last seen: 8 years, 2 months
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WBS post-PC: How long can I wait to inoculate?
#22485644 - 11/06/15 11:21 AM (8 years, 2 months ago) |
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I just finished PCing 5 pint jars of WBS but found out my spores won't be here for inoculation for another 3 days. Is it possible to wait that long or do I have to start from scratch. How do I preserve these jars for 3 days without them becoming unusable? Thanks!
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dryhub
Stranger

Registered: 10/20/15
Posts: 24
Last seen: 7 years, 10 months
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Re: WBS post-PC: How long can I wait to inoculate? [Re: Newcap]
#22485670 - 11/06/15 11:27 AM (8 years, 2 months ago) |
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if you did it correctly(if there is nothing to grow or contam) just but them in to fridge (to be sure there is no water vapor escape from jars
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: WBS post-PC: How long can I wait to inoculate? [Re: dryhub]
#22485826 - 11/06/15 12:00 PM (8 years, 2 months ago) |
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Quote:
dryhub said: if you did it correctly(if there is nothing to grow or contam) just but them in to fridge (to be sure there is no water vapor escape from jars
don't put them in the fridge. Fridges are full of nasty contams. Leave them in the pressure cooker if you can. Then resterilize when your spores arrive (although spores>grain is a bad idea) I'd make an LC first, or create a quarter pint grain jar from spores and perform G2Gs
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Newcap
Stranger
Registered: 11/06/15
Posts: 3
Last seen: 8 years, 2 months
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Re: WBS post-PC: How long can I wait to inoculate? [Re: PsilocyBen17]
#22486833 - 11/06/15 03:48 PM (8 years, 2 months ago) |
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I appreciate the feedback. Re-sterilizing is ok?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: WBS post-PC: How long can I wait to inoculate? [Re: Newcap]
#22486861 - 11/06/15 03:55 PM (8 years, 2 months ago) |
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Quote:
Newcap said: How do I preserve these jars for 3 days without them becoming unusable? Thanks!
Leave them on a shelf, they'll stay good for weeks when properly sterilized.
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Re: WBS post-PC: How long can I wait to inoculate? [Re: Psilosoulful]
#22486956 - 11/06/15 04:15 PM (8 years, 2 months ago) |
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i agree with Psilosoulful...just leave em on a shelf until the spores arrive. My quart rye jars sat for 2 weeks and didn't colonize (inoculated with spore syringe), so I crumbled up a pf cake and did grain to grain transfer to the quart jars (which hadn't contaminated) and had successful colonization. I would imagine if you attempt to re-sterilize the jars it could end up reducing the moisture content excessively
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: WBS post-PC: How long can I wait to inoculate? [Re: crackbaby]
#22487083 - 11/06/15 04:45 PM (8 years, 2 months ago) |
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Quote:
crackbaby said: I would imagine if you attempt to re-sterilize the jars it could end up reducing the moisture content excessively
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Parafaragaramus
Conquistador



Registered: 01/21/13
Posts: 446
Last seen: 2 months, 22 days
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Re: WBS post-PC: How long can I wait to inoculate? [Re: Psilosoulful]
#22488298 - 11/06/15 09:02 PM (8 years, 2 months ago) |
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I'd imagine they'd be good for at least two weeks if you have a proper filter and injection port. Every time I pc wbs I'm left with some condensation that takes a few days of shaking to reabsorb. I always make some jars around wednesday or thursday so I can innoc on saturday or sunday. Never had a problem that way. I'd imagine they'd still be good for a while after that
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: WBS post-PC: How long can I wait to inoculate? [Re: Parafaragaramus]
#22488314 - 11/06/15 09:05 PM (8 years, 2 months ago) |
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No need to refrigerate. That will probably just cause unwanted condensation. They should be fine for at least a couple weeks. WBS especially doesn't seem to have much problem with bacterial endospores, so it should keep well.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Newcap
Stranger
Registered: 11/06/15
Posts: 3
Last seen: 8 years, 2 months
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Re: WBS post-PC: How long can I wait to inoculate? [Re: Machiavelliavore]
#22490187 - 11/07/15 10:52 AM (8 years, 2 months ago) |
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Thanks very much!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: WBS post-PC: How long can I wait to inoculate? [Re: Newcap]
#22490250 - 11/07/15 11:03 AM (8 years, 2 months ago) |
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They can sit on a shelf at room temperature for a couple months if they were made and sterilised corectly. Don't put them in the fridge. That will just dry them out faster. Don't leave them in the PC. It's too damp in there. Just put them on a shelf.
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