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Invisibleuncommonsence
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When should I consider Re Inoculating?
    #22485559 - 11/06/15 11:05 AM (8 years, 2 months ago)

Hello all, I have knocked up 24 1/2 pint jars on 10/30/15 around 9 pm. Then knocked up 3 huge jars on 11/4/15 At about 5 am. Fairly amateur, So far I have grown 7 times or so my most recently some oysters and all I did was order petri dishes and cut out the wedges onto top of brf in jars in my home made glove box. When using MS I usually see signs in 3-5 days of go time. Currently well into day 7 and nothing at all. Last time something like this happened to me I waited about 16 days to see signs in only 1 jar in 1 inoculation spot and ultimately grew that out and started from scratch. Sooo... Knowing my understanding of how things work how long would you give this non life producing sideshow?

Jars were cool to touch

Sterile procedure was on point (Like Totally) Lol

Temps 79 in day time as low as 62 in evening. (Recently made makeshift heater)

Spores said to be about a year old

Jars were not necessarily packed but they were filled rather generously


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InvisibleInocuole
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Re: When should I consider Re Inoculating? [Re: uncommonsence] * 1
    #22485571 - 11/06/15 11:08 AM (8 years, 2 months ago)

I wouldn't re-inoculate anything ever without re-sterilizing and at that point you may as well start over so you don't mess up moisture content. :shrug:


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: Inocuole]
    #22485590 - 11/06/15 11:11 AM (8 years, 2 months ago)

Understood so basically at a cross between risk the possibility of all my jars starting tomorrow and throwing it out the window today for something more fruitful and vice versa on waiting forever and having new jars done today.

Yeah got a big ol bag of verm so no worries on being able to just whip out another 24. Maybe pack them lighter this time.


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Edited by uncommonsence (11/06/15 11:30 AM)


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OfflinePsilocyBen17
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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22485843 - 11/06/15 12:03 PM (8 years, 2 months ago)

wait and see. 79F might be slightly hot, as myc generates its own heat the internal temp is probably closer to 83, which is on the high end for myc.


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Invisibletahoe
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Re: When should I consider Re Inoculating? [Re: PsilocyBen17]
    #22486129 - 11/06/15 01:21 PM (8 years, 2 months ago)

Give it at least another week before doing anything. Everything slows down in the fall, assuming you are in the northern hemisphere


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OfflineLogicaL ChaosM
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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22486170 - 11/06/15 01:33 PM (8 years, 2 months ago)

Thats a lot of jars to shoot up.

How much spores did u use altogether?

using just a little spores per jar makes it a lot slower as two spores have to "mate" in the same area.

Could be conditions arent perfect in the jars, like too wet for example.


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OfflinePsilocyBen17
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Re: When should I consider Re Inoculating? [Re: tahoe]
    #22486172 - 11/06/15 01:33 PM (8 years, 2 months ago)

Quote:

tahoe said:
Everything slows down in the fall, assuming you are in the northern hemisphere




Why do you say this? Isnt the OP working indoors?


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OfflineLogicaL ChaosM
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Re: When should I consider Re Inoculating? [Re: PsilocyBen17]
    #22486195 - 11/06/15 01:40 PM (8 years, 2 months ago)

Most  people do not heat their homes/living spaces to 70F in the fall. Its just so expensive to heat a home/living space to that temp all the time.

Most homes i would say are in the 60Fs to save money and its more practical.

And 60-69F is colder than normal, but pretty nice for the ps cyan species.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: LogicaL Chaos]
    #22486287 - 11/06/15 02:01 PM (8 years, 2 months ago)

Im on the west coast and the room there in is in the sun all day :smile:

The print was rather small. I only put about 1/4 of a cc in each jar. When I first started growing I knocked up 24 jars with 2 ccs hehee 3 days healthy growth.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: uncommonsence] * 1
    #22495013 - 11/08/15 10:12 AM (8 years, 2 months ago)

Welp.. Inoculated 16 new jars yesterday with a new print this time didn't try to force as much substrate in jars as the first batch. I will keep you guys updated on germination.


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Offlinetetherface
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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22495058 - 11/08/15 10:27 AM (8 years, 2 months ago)

Quote:

uncommonsence said:
Welp.. Inoculated 16 new jars yesterday with a new print this time didn't try to force as much substrate in jars as the first batch. I will keep you guys updated on germination.



That couldve been your problem in the first place you never want to pack/force your pf sub and Ive had spores tak 2 weeks to germinate so id keep the other jars around for awhile


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: tetherface]
    #22495220 - 11/08/15 11:13 AM (8 years, 2 months ago)

Yeah the other jars were definitely too wet. I'm fairly certain it was because I tried to pack them with more sub than I should have.

I did save some of the dryer/better looking ones just out of curiosity when discarding them. Also there was def some bacterial contamination going on from excess moisture and probably a little too high temps as Ben17 pointed out. Witch once I noticed while throwing first one out didnt make me feel so bad about my thoughts on starting over too early. Going to keep this run on the cooler end of things. Also got another print on the way so we will have some more fun when that arrives.

Keep on making jars until the universe gives me what I need and more hehee

But yeah I warned you im fairly noobish hehee. Wish me luck :smile: and thanks for taking interest in my ventures.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: uncommonsence] * 1
    #22549130 - 11/19/15 09:38 PM (8 years, 2 months ago)

Ok. So signs of life were seen today on a completely separate start up from another print source done on the 17th. 8 jars made and several have some mycelium starting to take hold already.

Made 7 more jars today 4 of them being big and will inoculate in morning, also made another syringe for remaining 14 or so that I will be emptying cleaning and using tomorrow.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: uncommonsence] * 1
    #22567176 - 11/24/15 09:41 AM (8 years, 2 months ago)

Pictures because I have no life.








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OfflinePsilocyBen17
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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22570281 - 11/24/15 08:19 PM (8 years, 2 months ago)

Take the tinfoil off jabroni


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OfflineMrSturgill
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Re: When should I consider Re Inoculating? [Re: PsilocyBen17]
    #22570732 - 11/24/15 10:06 PM (8 years, 2 months ago)

:whathesaid:


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InvisibleAquaticPuzzle
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Re: When should I consider Re Inoculating? [Re: MrSturgill] * 1
    #22570755 - 11/24/15 10:13 PM (8 years, 2 months ago)

If those jars have lids and a dry verm barrier you should take that tin foil off and let your mycelium breathe.


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OfflineLogicaL ChaosM
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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22570850 - 11/24/15 10:46 PM (8 years, 2 months ago)

Good job man :thumbup:

And yeah, u could take the  foil off now. I like to leave mine loose on the top for a week cause im paranoid about infections. its up to you.


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PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms:shroomeryhead:| Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm :tombstone: || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏                                                         
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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: LogicaL Chaos]
    #22570931 - 11/24/15 11:17 PM (8 years, 2 months ago)

Fo sho thanks guys :smile: Yeah I decided to leave out the vermiculite barrier this time. It was not based on logic, seems every grow I tried with verm barrier in past failed. Most likely in hindsight because of old spores or lack of hydration time. Whatevz I fulfilled my own ridiculous conspiracy.

As long as it works I'm happy lol


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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22571206 - 11/25/15 02:12 AM (8 years, 2 months ago)

Little bit of a thread hijack, but since you mentioned you have dropped agar on top of brf jars for oysters before...

Did it seem to you like it took them *forever* to colonize?  And look like the myc spread across the top and around before digging down through the cake?


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Re: When should I consider Re Inoculating? [Re: Sivarted]
    #22571210 - 11/25/15 02:15 AM (8 years, 2 months ago)

Little iffy without a verm barrier btw, unless your lid has filters instead of open holes.  Might be ok with just the foil, but any air that goes up the foil to get in the jar can carry contams with it.

I just do 1/4 inch holes stuffed tight with poly, and inoculate through the poly.  Seems to be working pretty well, and lids are easy to make.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: Sivarted]
    #22571613 - 11/25/15 07:10 AM (8 years, 2 months ago)

Yeah honestly It took about 4 days to see signs of life forming on top and about another week before mycelium started going nuts. But at the same time it was very thin and non riz myc. They were done colonising about 6 weeks in. I didn't fruit them until well over 2 months was in Oregon and took them to Eugene with me to look for work and ultimately ended up in Tijuana where I fruited them out.


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Edited by uncommonsence (11/25/15 07:11 AM)


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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22571826 - 11/25/15 08:36 AM (8 years, 2 months ago)

Good luck.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: PsilocyBen17]
    #22571960 - 11/25/15 09:16 AM (8 years, 2 months ago)

Thanks Ben :smile: Things should go pretty smoothly from here on out. Yeah I imagine how my jars breathe from what little temp difference occurs between day and night time and the gas exchange from general temp differences inside and outside jars. Not too worried about it though. I will be def. taking some extra measures just to make the experience easier on myself in future and give better gae


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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22573495 - 11/25/15 02:34 PM (8 years, 2 months ago)

let me know how it goes without a verm barrier, I wouldn't have recommended it but my fingers are crossed now that you've inoculated.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: PsilocyBen17]
    #22573585 - 11/25/15 02:57 PM (8 years, 2 months ago)

Will do :smile:


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22589968 - 11/29/15 11:42 AM (8 years, 2 months ago)

So things are going as previously experienced with lack of verm layer thus far. No noticeable contaminations as of yet and mycelium seems to be digging life :smile:




Theres quite a big difference in myc growth on bigger jars compared to previous pic. taken 5 days ago :smile:



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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22591079 - 11/29/15 03:41 PM (8 years, 2 months ago)

Wow no verm layer? Better be careful when sitting down because you sir have some extremely large balls.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: MrSturgill]
    #22591177 - 11/29/15 04:11 PM (8 years, 2 months ago)

:freewilly:

Hehehee Seriously though its probably better If I do. Considering it would not put me out of a house and a home by taking that extra step but so far in my little experience this has always worked  :shrug:

Yes... yes... hurry up and wait.


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Edited by uncommonsence (11/29/15 04:18 PM)


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OfflinePsilocyBen17
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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22591768 - 11/29/15 06:34 PM (8 years, 2 months ago)

yea man, you already have verm to make BRF jars, so why not use a verm barrier? In BRF jars thats the only filter.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: PsilocyBen17]
    #22745091 - 01/06/16 05:40 PM (8 years, 24 days ago)

Quick question.... My fungi wont stay cracker dry and I hate applying heat to them multiple times to get to that point. Fan them on wire mesh for 24-48 hours usually splitting the stems because it hardly effective otherwise. Then I place my little make shift heater *a light bulb in a tall can in middle of 2 wire mesh platforms and blow warm air on them until there cracker dry mostly laying stems and caps on top of can scarcely to get them dry the rest of the way. But they keep going from crakcer dry to bendable and its pissing me off lol I dont have any jars laying around without holes in them.

Can I just leave the fan on the fuckers thus preventing them from sitting stag and getting mold from improper drying?


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Re: When should I consider Re Inoculating? [Re: uncommonsence]
    #22745128 - 01/06/16 05:48 PM (8 years, 24 days ago)

invest $20 in a food dehydrator. Made all the difference to me.


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Invisibleuncommonsence
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Re: When should I consider Re Inoculating? [Re: PsilocyBen17]
    #22745160 - 01/06/16 05:58 PM (8 years, 24 days ago)

Yeah that is def on my to do list. As of now can I prevent rotting/degradation/mold by keeping fan on them 24/7?


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