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OfflineCKeeper89
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Wbs contaminated again ..
    #22485451 - 11/06/15 10:41 AM (8 years, 2 months ago)

I just did my second attempt at doc's WBS. However I keep getting green contamination in a majority liked my jars, and I don't know why.

I followed docs tek to the T.

I cleaned the inoculation room very well and with Lysol air already, with no moving air.

I used alcohol flame in the syringe after each jar.


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OfflineDrCrumbs
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Re: Wbs contaminated again .. [Re: CKeeper89]
    #22485473 - 11/06/15 10:45 AM (8 years, 2 months ago)

Sounds like the issue is your syringe. It can be hard to do MS to grains. I've done it a bunch of times with success, and failures.

Prob go from BRF cake or Agar to grains.


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InvisiblebodhisattaMDiscordReddit
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Re: Wbs contaminated again .. [Re: DrCrumbs]
    #22485493 - 11/06/15 10:50 AM (8 years, 2 months ago)

Green is mold. Mold would be killed even if you only boil your jars a few minutes let alone sterilization. It's from you or your inoculant

Lysol a whole room is pretty much useless


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OfflinePsilocyBen17
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Re: Wbs contaminated again .. [Re: bodhisatta]
    #22485534 - 11/06/15 11:00 AM (8 years, 2 months ago)

Stupid question but are you using a SAB? The guy in the thread "THERE IS TRICH EVERYWHERE!" was not. No wonder.


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OfflineCKeeper89
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Re: Wbs contaminated again .. [Re: PsilocyBen17]
    #22485684 - 11/06/15 11:29 AM (8 years, 2 months ago)

I have not been using a SAB.
I am considering going to rye or using LC


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OfflinePsilocyBen17
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Re: Wbs contaminated again .. [Re: CKeeper89]
    #22485713 - 11/06/15 11:35 AM (8 years, 2 months ago)

consider using a SAB.


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OfflineCKeeper89
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Re: Wbs contaminated again .. [Re: PsilocyBen17]
    #22485848 - 11/06/15 12:04 PM (8 years, 2 months ago)

I will have to try that. I live in coastal nc, so mold is a big thing here.


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OfflineMoxyOx
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Re: Wbs contaminated again .. [Re: CKeeper89]
    #22485917 - 11/06/15 12:22 PM (8 years, 2 months ago)

You're breathing it onto the jars or moving it into your work area. They are everywhere buddy.


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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Invisibletahoe
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Re: Wbs contaminated again .. [Re: CKeeper89]
    #22486007 - 11/06/15 12:43 PM (8 years, 2 months ago)

Quote:

CKeeper89 said:
I have not been using a SAB.
I am considering going to rye or using LC




Well those are a couple of horrible ideas.
How would you inoculate the rye or lc?

So explain docs tech to us. What are you using for containers? Bags, jars? What is your filter media for the bags or jars.
How long are you pressure cooking?

Its either your pc, your filter, your syringe or you. I bet that you are doing something silly like opening the jar to squirt spores in


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflinePsilocyBen17
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Re: Wbs contaminated again .. [Re: tahoe]
    #22486165 - 11/06/15 01:32 PM (8 years, 2 months ago)

:whathesaid:

Not using a SAB is like fucking a hooker without a condom. You might get lucky once but if you do you shouldnt try it again.


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OfflineCKeeper89
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Re: Wbs contaminated again .. [Re: PsilocyBen17]
    #22486234 - 11/06/15 01:49 PM (8 years, 2 months ago)

I am using jars.
I used tryvek (over metal lid)
1/4 inch hole in center with polyfill
90 min pc time 15 psi
Micro tape over syringe hole


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OfflineCKeeper89
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Re: Wbs contaminated again .. [Re: CKeeper89]
    #22486254 - 11/06/15 01:53 PM (8 years, 2 months ago)

1/4 hole in the metal.  Not tryvek


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OfflinePsilocyBen17
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Re: Wbs contaminated again .. [Re: CKeeper89]
    #22486295 - 11/06/15 02:02 PM (8 years, 2 months ago)

yea of course you are using jars. The jars are inoculated inside a SAB.


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OfflineMoxyOx
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Re: Wbs contaminated again .. [Re: CKeeper89]
    #22488042 - 11/06/15 08:07 PM (8 years, 2 months ago)

Polyfill did not work for me. Mines had hidden contaminants. Get some quality tyvek or SFDs.

The only time you should be using polyfill is for tubs and making a breathable filter.


Edited by MoxyOx (11/06/15 08:07 PM)


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OfflineMachiavelliavore
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Re: Wbs contaminated again .. [Re: bodhisatta]
    #22488083 - 11/06/15 08:16 PM (8 years, 2 months ago)

Quote:

bodhisatta said:
Green is mold. Mold would be killed even if you only boil your jars a few minutes let alone sterilization. It's from you or your inoculant

Lysol a whole room is pretty much useless




:whathesaid:

Sounds like dirty inoculant.  Your best option is get some agar and drops your spores onto it.  You can try cornmeal plates or BRF agar substitute as well, though they aren't ideal for transfering away from contams or identifying them.  Mold should be easier to identify on these media than bacteria would be though.  :shrug:  http://www.shroomery.org/forums/showflat.php/Number/22252599/fpart/all/vc/1

If you want to work without an airbox use silicon injection ports.  Postal tyvek is good enough for a runs at least even if it isn't ideal.  Place foil over the lid, then when you're ready to inoculate, remove the foil when you shoot up so you don't have to dirty up the port with alcohol.  I doubt this will solve your problem though, dirty syringe almost certainly.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflinePsilocyBen17
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Re: Wbs contaminated again .. [Re: Machiavelliavore]
    #22488957 - 11/07/15 12:23 AM (8 years, 2 months ago)

Why not just build a SAB though?


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Invisibleazur
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Re: Wbs contaminated again .. [Re: PsilocyBen17]
    #22488973 - 11/07/15 12:29 AM (8 years, 2 months ago)

Because then he won't have any contams to cry about


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineCKeeper89
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Re: Wbs contaminated again .. [Re: azur]
    #22490606 - 11/07/15 12:31 PM (8 years, 2 months ago)

I will be using a SAB next time.


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