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Shromey
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Rye jars ready for slurry? [with picture!]
#22484425 - 11/06/15 04:29 AM (8 years, 2 months ago) |
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Hello my friends! I just bought a 23 qt Presto and a bunch of jars. I inoculated 3 jars about 1 or 2 weeks ago with my small pressure cooker. Directly after inoculation, i shaked one of the jars and two not. The one I shaked was really significant faster! I want to use the best of the 3 jars to inoculate a bunch of jars with a grain slurry.
To be sure that I do not have a contamination in my jars, I shaked all 3 jars 3 days ago. Now, 3 days after shaking the best jar looks like this:
The mycelium looks really dense, but of as you see not all of the grains are fully colonized tet. My main question is: Can I use this jar for a grain slurry, once all grains are colonized which I assume is in about 2 days, or should I wait a few days longer?
Edited by Shromey (01/09/16 06:53 AM)
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Machiavelliavore
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Re: Rye jars ready for slurry? [with picture!] [Re: Shromey]
#22484436 - 11/06/15 04:41 AM (8 years, 2 months ago) |
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I think eatyualive prefers small grains like millet for grain slurry, and prefers PF cakes or cornmeal plates to that now.
Making a slurry out of rye, which is a large grain known for endospores doesn't seem like a great idea, especially on your first real grow. In addition, I'm assuming you noc'd those jars with a spore syringe not an agar plate, which again, means slurry could amplify any small contaminant from the spore syringe massively.
Personally I think G2G would be the way to go. Keep anything nasty in the core of those grains instead of blending it up and spreading it all over. GLC would be another option, but it's dirtier and probably slower.
Really you should drop spores on agar for future jars. Barring that option G2G is probably your best. Check out the cornmeal plate LI on the tribal forum: http://www.shroomery.org/forums/showflat.php/Number/22252599/fpart/all/vc/1
Maybe drop some spores on a cornmeal plate and watch is closely, or transfer a colonized grain to a cornmeal plate, then remove the grain and blend the plate.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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tetherface
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Ive had lots of success using oats as slurry but you need to know 100% that your master is clean ie agar wedge inoculate because it sucks to dump out 30 jars after a slurry that got bacteria believe me ive done it...
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Shromey
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Re: Rye jars ready for slurry? [with picture!] [Re: tetherface]
#22485464 - 11/06/15 10:44 AM (8 years, 2 months ago) |
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Hmm okay thank you, I think I will really try g2g with the jar to inoculate 10 jars. Maybe I also make a slurry for 2 or 3 jars as an experiment.
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PsilocyBen17
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Re: Rye jars ready for slurry? [with picture!] [Re: Shromey]
#22485851 - 11/06/15 12:05 PM (8 years, 2 months ago) |
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whats the advantage of a grain slurry? Why not do G2G transfers? Less vectors for contams yo.
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Shromey
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Re: Rye jars ready for slurry? [with picture!] [Re: PsilocyBen17]
#22486135 - 11/06/15 01:24 PM (8 years, 2 months ago) |
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Faster colonization? I've read that it is possible to get fully colonized jars within 3 or 4 days! One guy spawned a mix of normal rye spawn and slurry to bulk substrate. Time from spawn to harvest was 13 days!
How fast do you think will be g2g transfer? It took about 2 weeks from inoculation with a syringe to the state of the photo.
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PsilocyBen17
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Re: Rye jars ready for slurry? [with picture!] [Re: Shromey]
#22486161 - 11/06/15 01:31 PM (8 years, 2 months ago) |
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Speed of colonization depends on many factors, but G2G is the fastest inoculation method. You can visually inspect jars for cleanliness, and you are starting with myc thats already been colonized.
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Machiavelliavore
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Re: Rye jars ready for slurry? [with picture!] [Re: PsilocyBen17]
#22486583 - 11/06/15 03:00 PM (8 years, 2 months ago) |
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Quote:
PsilocyBen17 said: Speed of colonization depends on many factors, but G2G is the fastest inoculation method. You can visually inspect jars for cleanliness, and you are starting with myc thats already been colonized.
It is certainly not unless you use a lot of grain. Colonizing that said lot of grain for g2g will take more time than colonizing a half pint of grain for slurry. I'd like to see you colonize 15-20 jars with g2g in 5 days. Might be possible with grass seed or millet due to the inoculation point count. Add to that breaking up grains in jars is nervewracking and pouring grain is harder to do that pouring liquid.
I can store a few colonized cakes in my fridge and turn that into as much spawn as I want. It's a lot more practical than storing quarts of grain in the fridge for every culture I think might be good.
Anyway, whatever, g2g is better for this dude until he gets some agar or at least cornmeal plates, but slurry is dope as fuck.
OP, look at this. Dropping spores on one of these would be a better option than slurrying grains from MS IMO. Make a couple of plates, observe, maybe test one on a small quantity of grain or on a PF cake: : http://www.shroomery.org/forums/showflat.php/Number/22252599/fpart/all/vc/1
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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PsilocyBen17
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I wouldnt store anything in the fridge. Fridges are nasty places. Colonizing 15 jars in 5 days is easy enough if you use a high amount of grain from the Master. a 50/50 ratio for example would be no problem.
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Machiavelliavore
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Re: Rye jars ready for slurry? [with picture!] [Re: PsilocyBen17]
#22486863 - 11/06/15 03:55 PM (8 years, 2 months ago) |
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... And those would be colonized by a2g on 5 quart would probably take 10 days. a2g on a half pint will be faster, and additionally, when I make the slurry, I can make another half pint with said slurry that can inoculate another 20 jars and another half pint in 5 days. This all occurs in one usage of the SAB as well, instead of requiring two separate transfers.
G2g takes like 15 days at least from the point where you have the desire to create X amount of spawn to when you have it, unless you keep quarts of every culture you're interested lying around. Any time I have a culture of interest, chuck a wedge in a half pint. Ready to rock n roll if I wanna try it in 5 days.
And you put it in the fridge cause it has a filter or simply use twist tops with closed lids? Nobody freaks out about putting plates, slants, or grain jars in the fridge afaik.
Anyway, I'm not trying to start any shit here. It's just a fact that slurry has incredibly high inoculation potential from an incredibly small amount of source material. A tablespoon of slurry diluted at one to two, so really a teaspoon of grain, can do what a third of a cup of small grains can do in g2g. That doesn't mean you have to use it or g2g is bad. There are risk factors with slurry, but efficacy is not one them.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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blindingleaf
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Registered: 07/19/13
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Re: Rye jars ready for slurry? [with picture!] [Re: Shromey]
#22487193 - 11/06/15 05:07 PM (8 years, 2 months ago) |
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Quote:
Shromey said:
 My main question is: Can I use this jar for a grain slurry
that doesn't look like a jar i would use for a slurry or g2g. i would spawn it if anything that is not healthy recovery…those thick knots are weird.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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PsilocyBen17
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Re: Rye jars ready for slurry? [with picture!] [Re: blindingleaf]
#22488980 - 11/07/15 12:31 AM (8 years, 2 months ago) |
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hi BL, congrats on the tag.
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blindingleaf
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Re: Rye jars ready for slurry? [with picture!] [Re: PsilocyBen17]
#22489350 - 11/07/15 06:48 AM (8 years, 2 months ago) |
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Yo Ben, thanks bud
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Machiavelliavore
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Re: Rye jars ready for slurry? [with picture!] [Re: blindingleaf]
#22491440 - 11/07/15 03:36 PM (8 years, 2 months ago) |
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I thought he meant that he shook it after 20-30% with MS and it was now getting close to full colonization.
I'm not familiar with what rye looks like colonized though. I didn't see anything that seriously concerned me.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Pastywhyte
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Registered: 09/15/12
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People here have lower standards compared to leaf. I personally agree with him 100% but people have different experiences. For me simply to have a jar of myc is not enough. I would either spawn or top fruit that. Not expand. Certainly when expanding with slurry you want too have a very clean jar of spawn. Tiny amounts of bacteria could have a hey day in a slurry expansion.
It's good to have a grounding in what different grains look like when colonized and the different ways the myc can look on said grains depending on the culture.
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