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Shop: Left Coast Kratom Kratom Powder For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order

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Offlineragadinks
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Lower the PH of agar ?
    #2248399 - 01/17/04 03:04 PM (17 years, 10 months ago)

what can I do in order to lower the PH of my agar? I have prepared DFA with water that was rinsed through peat ( PH 4 ) but the agar still has a PH of 6. I need a PH about 4 in order to try out if hypholoma capnoides is growing better at this PH level.


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Offlinellamaboy
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Re: Lower the PH of agar ? [Re: ragadinks]
    #2248855 - 01/17/04 06:30 PM (17 years, 10 months ago)

this probably isnt' the safest thing to do, but you could maybe use an acid? go through your DFA tek like normal, wait for it to cool a little, and then drop in like a little 1N sulfuric acid or something? i'd stay away from HCl might make growing something kinda difficult with that chloride floating around.


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InvisibleYarry
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Re: Lower the PH of agar ? [Re: llamaboy]
    #2248858 - 01/17/04 06:31 PM (17 years, 10 months ago)

i duno how good it would be, but while on the topic of acid, why not just add an acidic food item? like vinegar or lemon juice?


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Offlineragadinks
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Re: Lower the PH of agar ? [Re: Yarry]
    #2248867 - 01/17/04 06:35 PM (17 years, 10 months ago)

hmmmh, vinegar sounds good. The other stuff seems to aggressive to me !


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InvisibleYarry
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Re: Lower the PH of agar ? [Re: ragadinks]
    #2248882 - 01/17/04 06:40 PM (17 years, 10 months ago)

let me know if it works or not


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Offlinellamaboy
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Re: Lower the PH of agar ? [Re: Yarry]
    #2248941 - 01/17/04 06:58 PM (17 years, 10 months ago)

well, the only reason that i suggested something like 1N sulphuric acid is that it would only take a drop or two to lower the pH to about 4...whereas with vinegar or lemon juice you'll need considerably more thus you'd have to prepare the agar a little more differently. you'd most likely have to use a little more DFA per litre in order to get a good agar plate.


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Offlinecanid
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Re: Lower the PH of agar ? [Re: llamaboy]
    #2249018 - 01/17/04 07:21 PM (17 years, 10 months ago)

not too differently, you;'d just have to add enough of your acetic acid rich liquit to adjust the ph properly, while leaving out that same volume of water.


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InvisibleYarry
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Re: Lower the PH of agar ? [Re: canid]
    #2249023 - 01/17/04 07:22 PM (17 years, 10 months ago)

itll probably smell funky tho! :lol:


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Offlinecanid
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Re: Lower the PH of agar ? [Re: Yarry]
    #2249038 - 01/17/04 07:26 PM (17 years, 10 months ago)

with vinigar i'd say yep, but not too bad. with citrus juice it wouldn't be bad at all.


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Offlineragadinks
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Re: Lower the PH of agar ? [Re: ragadinks]
    #2249137 - 01/17/04 08:06 PM (17 years, 10 months ago)

when I add it before autoclaving will the PH still be the same after autoclaving ? I mean will it stand the heat ?


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InvisibleYarry
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Re: Lower the PH of agar ? [Re: ragadinks]
    #2249139 - 01/17/04 08:08 PM (17 years, 10 months ago)

yeah it should, acetic acid (vinegar) and citric acid (lemons) both have high melt/boil points, it should stay but im not certain


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Offlineragadinks
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Re: Lower the PH of agar ? [Re: Yarry]
    #2249177 - 01/17/04 08:39 PM (17 years, 10 months ago)

Citric Acid seems to be ok. Here is some chemical data:


Citric Acid:

Melting Point: 153-154 deg C (307.4-309.5 deg F) (anhydrous) (2,16,17)
Boiling Point: Decomposes at 175 deg C (347 deg F)
source

Acetic Acid:

Melting point: 16.7 C
Boiling point: 118 C
source


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InvisibleYarry
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Re: Lower the PH of agar ? [Re: ragadinks]
    #2249192 - 01/17/04 08:50 PM (17 years, 10 months ago)

shit i thot acetic was higher.. maybe im thinking of formic acid, which doesnt have a really easy source (its the chemical compound fire ants have in their jaws to make u hurt)


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Offlineragadinks
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PH 4 -> Agar does not solidify ! [Re: Yarry]
    #2252281 - 01/19/04 10:40 AM (17 years, 10 months ago)

I have now made 3 bottles of agar. One has PH 5 and the other two have PH 4. But the funny thing is that the agar in the two bottles with PH 4 does not solidify whereas it does at PH 5.


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InvisibleYarry
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Re: PH 4 -> Agar does not solidify ! [Re: ragadinks]
    #2252327 - 01/19/04 11:19 AM (17 years, 10 months ago)

whatd you use to drop the pH?


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Offlineragadinks
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Re: PH 4 -> Agar does not solidify ! [Re: Yarry]
    #2252370 - 01/19/04 11:45 AM (17 years, 10 months ago)

I have found some powder of citric acid as it is used for cooking. It worked very well - just added a few crystals.


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InvisibleYarry
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Re: PH 4 -> Agar does not solidify ! [Re: ragadinks]
    #2252376 - 01/19/04 11:48 AM (17 years, 10 months ago)

hmm, is it a jelly at all or no? and what did you substitute out of the receipe to put the crystals in?


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Offlineragadinks
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Re: PH 4 -> Agar does not solidify ! [Re: Yarry]
    #2252418 - 01/19/04 12:11 PM (17 years, 10 months ago)

Quote:

hmm, is it a jelly at all or no?




No, it's liquid. Not jelly at all.

Quote:

and what did you substitute out of the receipe to put the crystals in?




I did not substitute anything out of the recipe. Since I did not add any liquid I assumed that the amount of nutrients and agar should stay the same.


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InvisibleYarry
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Re: PH 4 -> Agar does not solidify ! [Re: ragadinks]
    #2252446 - 01/19/04 12:24 PM (17 years, 10 months ago)

ok, well heres what i would do, mix the crystals in with the liquid you put in until its around pH 2, (you will have to experiment until you get the proper final pH) and then add that mix to all your dry ingrediants. hopefully that should help the jelly set.. lemme know!


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Offlineragadinks
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Re: PH 4 -> Agar does not solidify ! [Re: Yarry]
    #2252488 - 01/19/04 12:42 PM (17 years, 10 months ago)

Why do you think that would work ? Why should adding the liquid with PH 2 to the dry ingredients make the agar solidify ?


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Shop: Left Coast Kratom Kratom Powder For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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