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Invisibletahoe
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Re: Bags! Bags! Bags? [Re: azur]
    #22486092 - 11/06/15 01:12 PM (8 years, 2 months ago)

Quote:

azur said:
Quote:

tahoe said:
Quote:

azur said:
:thumbdown:

Bags are awesome!....for g2g.
They are ok for a2g and lc.
They suck for ms syringe, as do jars.

You need to g2g from a colonized jar. MS syringe in a bag (because it's so much grain) will almost always stall out due to bacteria.




I grew all summer long using the same three spore syringes. I use 1/4th-1/2th cc max per bag. I am using the medium sized bags with 10-12 heaping cups of grain in each one. I had less than 1% contams, 0 bags stall.
According to my experience I would call your statement false.

For shits and giggles I cooked up two xl spawn bags. I used less than a half cc on each bag. I think it's been a month but they both are fully colonized now.

I would imagine that more multi spore would be better. Faster colonization is almost always preferred.



Yea. I got a problem. Firstly, this post is full of lies. Anytime anyone says i had less than blah blah blah percent contam rate, they're talking out their ass. If he was actually tracking data to know what exact percent rate he lost to contams, he wouldn't be spewing out the nonsense i see all over the boards.
Secondly, not one mother fucker on this site experienced less than one percent contam rate this summer. Not one.
Thirdly, more spore solution is not what you do.


Tahoe, this is nothing personally against you. I kinda think you're alright. You're just a bullshitter. And that's cool by me. Just play it how it is.




I just noticed this post. Explain full of lies or even exaggeration. Sure I have had contams this summer, hell I just trashed a bag two days ago. But it wasn't related to these three spore syringes. You want to know something else. I never once flammed a needle nor inoculated a bag in a box or a hood. I even refilled a syringe with sterile water once it got down to 2cc's.

Anyways please explain FULL OF LIES. And you are right, I did not keep data on my contams. Just going off of memory


--------------------
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OfflinecronicrFacebook
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Re: Bags! Bags! Bags? [Re: tahoe]
    #22486207 - 11/06/15 01:42 PM (8 years, 2 months ago)

:lol::standingby:


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Offlinemushpunx
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Re: Bags! Bags! Bags? [Re: cronicr]
    #22486267 - 11/06/15 01:56 PM (8 years, 2 months ago)

Really dude? Never flamed a syringe? I know you like to sterilize your bags pre sealed and inoculate thru the side with LI or whatever. I can see that done in open air just fine

But I would think an un flamed needle would be bad! :lol:


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OfflinetaGyo
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Re: Bags! Bags! Bags? [Re: mushpunx]
    #22486274 - 11/06/15 01:57 PM (8 years, 2 months ago)

You don't flame your needles Tahoe...?


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InvisibleInocuole
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Re: Bags! Bags! Bags? [Re: taGyo]
    #22486288 - 11/06/15 02:01 PM (8 years, 2 months ago)



--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleAdden
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Re: Bags! Bags! Bags? [Re: taGyo]
    #22486408 - 11/06/15 02:27 PM (8 years, 2 months ago)

Quote:

taGyo said:
You don't flame your needles Tahoe...?




Flaming needles once and using 70% iso in between jars or bags will prevent cross contamination. I recall someone mentioning it's better because certain contams need that little bit of water to "come out and die" on sterilization. Can anyone confirm?


If I was ever to grow I would imagine that I'd do it, simply because I'd likely run into a situation of 10 fine jars and 2 ruined ones.


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Invisibleazur
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Re: Bags! Bags! Bags? [Re: tahoe]
    #22486462 - 11/06/15 02:36 PM (8 years, 2 months ago)

I already explained the full of lies part in that post. Less than 1 percent means you lost 1 out of 101 bags. Did you use that many bags? 
Fwiw, I rarely flame anything. I pc em instead


--------------------


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Re: Bags! Bags! Bags? [Re: azur]
    #22486509 - 11/06/15 02:47 PM (8 years, 2 months ago)

Dys flaming between each jar is better as heat sterilizes both inside and outside the needle


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OfflinetaGyo
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Re: Bags! Bags! Bags? [Re: Adden]
    #22486518 - 11/06/15 02:49 PM (8 years, 2 months ago)

Quote:

Dys said:
Quote:

taGyo said:
You don't flame your needles Tahoe...?




Flaming needles once and using 70% iso in between jars or bags will prevent cross contamination. I recall someone mentioning it's better because certain contams need that little bit of water to "come out and die" on sterilization. Can anyone confirm?


If I was ever to grow I would imagine that I'd do it, simply because I'd likely run into a situation of 10 fine jars and 2 ruined ones.



Well you're right about flaming but not about alcohol:

Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.




I actually touched an iso soaked glove to agar and got contams:

http://www.shroomery.org/forums/showflat.php/Number/20969519


--------------------
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InvisibleAdden
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Re: Bags! Bags! Bags? [Re: taGyo]
    #22486552 - 11/06/15 02:56 PM (8 years, 2 months ago)

I just checked and RR said 70%, and not flame every needle.

If you guys say differently though I'll go with that. It's how I'd always do it before, and just stumbled across that information the other day.


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Re: Bags! Bags! Bags? [Re: Adden]
    #22486585 - 11/06/15 03:01 PM (8 years, 2 months ago)

If your doing cakes flaming between each jar is almost a must, shit tends to get in the needle with grains i haven't seen any huge issues but i dont' touch anything with the syringe when i do grains..i line them up and inoc fro a few inches back(a  syringe could probably shoot 20 feet lol)


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I'm tired do me a favor


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InvisibleAdden
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Re: Bags! Bags! Bags? [Re: cronicr]
    #22486589 - 11/06/15 03:02 PM (8 years, 2 months ago)

Good to know if I ever grow anything besides edibles again! Thank you.


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OfflinetaGyo
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Re: Bags! Bags! Bags? [Re: Adden]
    #22486692 - 11/06/15 03:23 PM (8 years, 2 months ago)

Quote:

Dys said:
I just checked and RR said 70%, and not flame every needle.

If you guys say differently though I'll go with that. It's how I'd always do it before, and just stumbled across that information the other day.



Weird, I've never seen RR use alcohol :shrug:

He doesn't do it in the Lets Grow Mushrooms video

http://www.mushroomvideos.com/BRF-Pf-Tek


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum


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Re: Bags! Bags! Bags? [Re: taGyo]
    #22486699 - 11/06/15 03:24 PM (8 years, 2 months ago)

He doesnt' but advises on it all the time(or use to)


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinetaGyo
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Re: Bags! Bags! Bags? [Re: cronicr]
    #22486708 - 11/06/15 03:26 PM (8 years, 2 months ago)

Weird,

I'd never do it but that man has grown more mushrooms then I've seen :shrug:


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Re: Bags! Bags! Bags? [Re: mushpunx]
    #22488015 - 11/06/15 08:00 PM (8 years, 2 months ago)

Quote:

mushpunx said:

Take a clean agar culture and sterilized syringe full of water. Flame needle, squirt water onto plate. Agitate myc, pull myc water into syringe. Then you have a clean LI to inject thru the ports.





Good tip. I'm not quite set up to do regular Karo LC, but I can swing this.

How do you agitate the water after squirting it onto the plate? Do you stir with the tip of the needle or swirl the plate?

I assume you can use the plate multiple times, over the course of weeks or even a couple of months, as the mycelium will just grow back . . .  cool


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InvisibleAdden
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Re: Bags! Bags! Bags? [Re: RJ Tubs 202]
    #22488551 - 11/06/15 10:01 PM (8 years, 2 months ago)

Agitate your liquid culture*
then draw that into your syringe.


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OfflineRJ Tubs 202
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Re: Bags! Bags! Bags? [Re: Adden]
    #22489015 - 11/07/15 01:01 AM (8 years, 2 months ago)

(Am familiar with general LC handling, but haven't tried this little trick of making an LC from a plate directly with a syringe. Gonna try this soon!)

One final thought to share, and I'll let this thread drift into the ether . . .

This is a poor man's version of making an aqueous agar slurry with a blender (one of those fancy metal ones the pros use). I used to do this years ago and it worked really well.

I'd PC a kitchen sieve,
   
                           

and a spoon, water, and a jar (that would hold the sieve on top)

In a hood I'd pop the agar disc out of a plate, throw it in the sieve, and with the spoon mash it through the screen. Then would wash the material into the jar below, and distribute to grass seed or grain.

Each plate made a slurry of a few hundred tiny 1/16 inch cubes    :laugh:


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Offlinemushpunx
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Re: Bags! Bags! Bags? [Re: RJ Tubs 202]
    #22489081 - 11/07/15 02:00 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
Quote:

mushpunx said:

Take a clean agar culture and sterilized syringe full of water. Flame needle, squirt water onto plate. Agitate myc, pull myc water into syringe. Then you have a clean LI to inject thru the ports.





Good tip. I'm not quite set up to do regular Karo LC, but I can swing this.

How do you agitate the water after squirting it onto the plate? Do you stir with the tip of the needle or swirl the plate?

I assume you can use the plate multiple times, over the course of weeks or even a couple of months, as the mycelium will just grow back . . .  cool





You just kinda swish the needle tip over the myc. Its pretty soft and fluffy.
I would only use the plate once for this. Its not a big deal as you can just transfer a wedge to a new blank plate right before hand, that will grow out in a few days.

You don't want to get into LC. Not yet. Wait till you are more experienced, and even then it needs to be inoculated with a clean culture on agar.


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InvisibleBuck513

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Re: Bags! Bags! Bags? [Re: RJ Tubs 202]
    #22489123 - 11/07/15 02:46 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
(Am familiar with general LC handling, but haven't tried this little trick of making an LC from a plate directly with a syringe. Gonna try this soon!)

One final thought to share, and I'll let this thread drift into the ether . . .

This is a poor man's version of making an aqueous agar slurry with a blender (one of those fancy metal ones the pros use). I used to do this years ago and it worked really well.

I'd PC a kitchen sieve,
   
                           

and a spoon, water, and a jar (that would hold the sieve on top)

In a hood I'd pop the agar disc out of a plate, throw it in the sieve, and with the spoon mash it through the screen. Then would wash the material into the jar below, and distribute to grass seed or grain.

Each plate made a slurry of a few hundred tiny 1/16 inch cubes    :laugh:




I've never heard of that. Mashing agar through a metal screen to increase inoculation points doesn't sound like a good idea to me...


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