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InvisibleSupalemonhaze
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Trich on everything!!!! [Re: ConsciousFungi]
    #22486304 - 11/06/15 02:06 PM (8 years, 2 months ago)

Quote:

PsilocyBen17 said:
Quote:

Supalemonhaze said:

Edit: also I seen the designs of cardboard SABs. Cardboard is straight as far as my 'ignorant' mind remembers





Sorry for knocking you man, I just don't think its wise to be championing open air inoculations when SAB are so easy to make. The reason people post on the shroomery is so that noobs can have fun on their first grow instead of experiencing catastrophe. Even if by some miracle you perform open air innoculations successfully, it is not the norm, and you should expect serious contams using that technique.

Not sure what you mean about cardboard being straight?

Keep us posted on how your 26 jars do.



Quote:

PsilocyBen17 said:
PS: I'm confused. Why are you claiming you've been successful with open air inoculation if your 26 jars are still colonizing?




It's quite alright I know its the worst way to do it ( as I pointed out at the very start to op) but for some equipment is not as easy to get as one might think. Its not a miracle at all its just proper technique. By no means I am a professional, one look at the amount of contams I get on agar ( inside SAB) and you'll know. I just have more experience with the grains. When you really think about it its a hole a few mm's wide ( barely enough for syringe) which is immediately blocked when I inoculate and covered right after.

Also I said that they were "successful" because its been over a week since I inoculated so its pretty much safe to say any mold spores which could have entered would have germinated already like the ones I tried to ID in the other thread.

Good day/night to you. ( wont be for me just starting night shift at work :horrified:)



Quote:

ConsciousFungi said:
Quote:

Supalemonhaze said:
Quote:

ConsciousFungi said:
The trich originates from both the top and bottom of the jars.





I just noticed this. If you did not shake the jar after inoculation then for sure it was not from the open air inoculation. Can you see if the trich originates from the path the spore solution took?





I lightly shook the jar to resettle the wbs.  The trich was much more colonized at the bottom, but did seem to originate from a single seed at the top/mid-top each time.




Im afraid there is no knowning where it originated from then. When using a MS syringe people usually reccomend not to shake the jars so they will be closer together and colonise faster. Remember the faster the mycelium colonises the less chance of contams you have ( I never did MS). If I had this much trouble with my inoculations I would first get a good SAB to work in ( might wanna try the big bag tek there is floating around the forums somewhere) and second switch to agar to see where the problem is coming from. Goodluck to you mate.


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OfflinePsilocyBen17
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Re: Trich on everything!!!! [Re: Supalemonhaze]
    #22486317 - 11/06/15 02:09 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:



Also I said that they were "successful" because its been over a week since I inoculated so its pretty much safe to say any mold spores which could have entered would have germinated already like the ones I tried to ID in the other thread.



.




This isn't true. Mold can show up at any time. Many jars contam at 90% colonization.


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InvisibleSupalemonhaze
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Re: Trich on everything!!!! [Re: PsilocyBen17]
    #22488853 - 11/06/15 11:34 PM (8 years, 2 months ago)

Guess we'll wait and see wont we :smile: yes I did have a couple of jars which got infected late in colonisation ( the quart jar in my thread is one) but its safe to say that ill have most of my remaining 23 jars. Late contam germination only happened twice so far so im not really worried


Edited by Supalemonhaze (11/06/15 11:42 PM)


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OfflineConsciousFungi
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Re: Trich on everything!!!! [Re: Supalemonhaze]
    #22500330 - 11/09/15 12:45 PM (8 years, 2 months ago)

So I think 2/4 syringes were actually contaminated.  That seems like a very high ratio for a trusted sponsor, but I inoculated 4 jars by transferring pieces of a brf cake and used the 1cc I had left to inoculate one other jar.  The 4 jars with brf cake have not contaminated, despite actually opening the lids on them.  The other jar using the syringe has a very bad trich infection already.

Thank you all for helping me!  I doubt the brf transfer would have been successful without my new SAB.


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OfflinePsilocyBen17
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Re: Trich on everything!!!! [Re: ConsciousFungi]
    #22501028 - 11/09/15 04:03 PM (8 years, 2 months ago)

When you performed the BRF transfer did the jar you were transferring to have a dry verm layer? If so this is your problem.

BRF doesnt work well for G2G transfers, you'd have better success using grain in your SAB.


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InvisibleSupalemonhaze
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Re: Trich on everything!!!! [Re: PsilocyBen17]
    #22501043 - 11/09/15 04:06 PM (8 years, 2 months ago)

He said only syringe ones got contamed. If you remove verm layer it shouldn't really contam IMO.

Edit: the only setback with using brf for g2g style is that you have to break it up. Its a sub just like grains. Both do the same thing with the same amount of risk.


Edited by Supalemonhaze (11/09/15 04:15 PM)


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OfflinePsilocyBen17
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Re: Trich on everything!!!! [Re: Supalemonhaze]
    #22501064 - 11/09/15 04:11 PM (8 years, 2 months ago)

This thread is starting to annoy me. People shouldn't blame their setup when it is their technique and ideas that are at fault.


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Trich on everything!!!! [Re: PsilocyBen17]
    #22501099 - 11/09/15 04:17 PM (8 years, 2 months ago)

Lol from your own words you said brf is less suseptabile for contams than grains however you inoculate. And you say that the syringe is ok?

Syringes are known to carry contams just ask anyone.

If thread is starting to annoy you it's because it's showing you how flawed your thinking is.


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OfflinePsilocyBen17
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Re: Trich on everything!!!! [Re: Supalemonhaze]
    #22501147 - 11/09/15 04:26 PM (8 years, 2 months ago)

brf is less susceptible to contams and more "noob friendly" than grains because of the dry verm layer but of course its going to contam during an open air transfer (especially without the verm layer).

I've never seen anyone on this site suggest BRF for a G2G transfer using BRF (even inside a SAB). The reason people use grain is for the multiple inoculation points (colonized grain kernels). For this reason a BRF cake is a poor candidate for G2G's.

sponsors are known for their cleanliness. 4/4 dirty syringes seems unlikely to me.  If the vendor was a sponsor I would hesitate to blame them for the contam and lean towards the OPs technique...


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InvisibleShroomspective
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Re: Trich on everything!!!! [Re: PsilocyBen17]
    #22501179 - 11/09/15 04:30 PM (8 years, 2 months ago)

:whathesaid:


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InvisibleSupalemonhaze
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Re: Trich on everything!!!! [Re: PsilocyBen17]
    #22501230 - 11/09/15 04:39 PM (8 years, 2 months ago)

Quote:

PsilocyBen17 said:
brf is less susceptible to contams and more "noob friendly" than grains because of the dry verm layer but of course its going to contam during an open air transfer (especially without the verm layer).

I've never seen anyone on this site suggest BRF for a G2G transfer using BRF (even inside a SAB). The reason people use grain is for the multiple inoculation points (colonized grain kernels). For this reason a BRF cake is a poor candidate for G2G's.

sponsors are known for their cleanliness. 4/4 dirty syringes seems unlikely to me.  If the vendor was a sponsor I would hesitate to blame them for the contam and lean towards the OPs technique...




Dirty syringes are not unheard of. One print makes a few syringes if the print was dirty it wouldnt be a suprise a number of those syringes were contamed. Of course it doesn't mean the user shouldn't pick apart his technique to see if it is at fault. Personally I would take it to agar.


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InvisibleSupalemonhaze
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Re: Trich on everything!!!! [Re: PsilocyBen17]
    #22501284 - 11/09/15 04:47 PM (8 years, 2 months ago)

Quote:

PsilocyBen17 said:


I've never seen anyone on this site suggest BRF for a G2G transfer using BRF (even inside a SAB). The reason people use grain is for the multiple inoculation points (colonized grain kernels). For this reason a BRF cake is a poor candidate for G2G's.






On this you are right people do not suggest it but I have seen people use it. Saw a post few days ago where this guy uses a fork to inoculate jars from a master brf cake. Ill try to find it after work. My phone is dying 0.0


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OfflinePsilocyBen17
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Re: Trich on everything!!!! [Re: Supalemonhaze]
    #22501357 - 11/09/15 04:58 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:


Dirty syringes are not unheard of. One print makes a few syringes if the print was dirty it wouldnt be a suprise a number of those syringes were contamed. Of course it doesn't mean the user shouldn't pick apart his technique to see if it is at fault. Personally I would take it to agar.





Dirty syringes from a sponsor aren't unheard of, but they are rare. 4/4 dirty syringes? Thats like encountering a wild Mew and catching it with a normal Pokeball.  :slowpoke:


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Trich on everything!!!! [Re: PsilocyBen17]
    #22501414 - 11/09/15 05:06 PM (8 years, 2 months ago)

Im sorry catch a what with a what?

:freshwtf:


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Trich on everything!!!! [Re: Supalemonhaze]
    #22501419 - 11/09/15 05:06 PM (8 years, 2 months ago)

:ahahaha:

Edit: jokes aside though. Even if a vendor has a spotless track record. If something happens that contams a print chances are a good number of syringes made from that print will carry some kind of contaminate.

So far I have always been amazed at how many contaminated syringes vendors send and ultimately have to replace from their customers.


Edited by Supalemonhaze (11/09/15 05:17 PM)


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OfflineConsciousFungi
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Re: Trich on everything!!!! [Re: Supalemonhaze]
    #22502122 - 11/09/15 07:09 PM (8 years, 2 months ago)

Wow!  So it was only 2/4 syringes that may have been contaminated.  I know brf is not the best for g2g, but I only had 1cc of ms left. 

The 4 jars inoculated with the brf currently do not have any contamination.  The jar inoculated with the ms syringe is now full of trich.  I understand one jar isnt much for repeated results to test whether or not the SAB fixed my issue.  That being said, for 4 g2g transfers to make it and not the single ms jar, I would say its very possibly contaminated.

I also had two other jars, which I inoculated at the beginning of this thread, in open air with another ms syringe (the same one used for the brf cake).  Both of those jars are also currently free of contamination.


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InvisibleSupalemonhaze
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Re: Trich on everything!!!! [Re: ConsciousFungi]
    #22503381 - 11/10/15 02:27 AM (8 years, 2 months ago)

Thats cool man. Goodluck with fruiting them. Dont forget to post some pics of harvest. That's the best part :thumbup:


Found that thread where he uses brf for masters. Not exactly sure why though.

https://www.shroomery.org/forums/showflat.php/Number/11353633


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