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OfflineMachiavelliavore
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Registered: 12/08/14
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Re: Agar isolation and fruiting (ALL) [Re: Erik77johansson77]
    #22482253 - 11/05/15 06:07 PM (8 years, 2 months ago)

Couple methods I've seen that look good:

Any sort of invitro grow, brf, straight grains, or some kind of other bulk sub.  Check out the violet invitro thread.  Grains are peferable since inoculating BRF with agar sucks.

Only issue with muda bottles is that you wouldn't really want to make LI for 8+ cultures.  You can try agar, but it will still be slow.  You can inoculate with grains, but at that point you might as well just spawn them.

Another method that I use is to a2g a single quart of spawn, then spawn it to two quarts of coir in a $1 foil lasagnia pan with a plastic lid.  Shotgun the lid with holes every 2.5" with a soldering iron, and use a piece of trash bag for liner.  Mist 1-2x a day, works very well.  Due to the high FAE of these things, use a casing.  Dollartree has them cheap, you can also pick'm up at target/walmart/etc... for a bit more.  At that point you might as well grab a 15qt sterilite and make a 3qt sub monotub.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinemushpunx
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Registered: 04/20/14
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Re: Agar isolation and fruiting (ALL) [Re: Machiavelliavore]
    #22482354 - 11/05/15 06:31 PM (8 years, 2 months ago)

To inoculate BRF with agar you don't have to drop a wedge and use GE lid, you can sterilize a syringe of water, squirt some water onto your culture and then agitate with the flame sterilized needle and suck the myc water up for a fast LI
I


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Amateur Mycologists United
AMU Q&A


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Agar isolation and fruiting (ALL) [Re: mushpunx]
    #22483179 - 11/05/15 09:11 PM (8 years, 2 months ago)

Quote:

mushpunx said:


This is why you don't want to just end up with one isolate from a culture.

You should keep transferring until you see sectoring (like in pic) and then take a transfer from *each* sector and then keep making transfers on each untill you have a whole bunch of isolates.

You should aim to end up with at Least 10 isolates from a culture to test out. If you just have one you have nothibv to compare it to, and unless your lucky it might be garbage. Usually 1 out of 10 is a keeper




Yea at first I just got so blinded with wanting an isolate that I just aimed on getting one. Then I saw RR's vids and realised how much of a fuck up im making. Lucklily I still had all my plates and the transfers I had were still sectoring it was easy to get back on the right track


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