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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Help me pin the tail on the contaminated donkey.
    #22479739 - 11/05/15 07:18 AM (8 years, 2 months ago)

Sup people. I got some contams I wish to id before I get rid of the grains/agar. I just hate not knowing what they are so hopefully you guys will know. If you need better photos just ask ill take them again.

contam #1 I was thinking cobweb?


contam #2 this is really peaking my interest as ive never seen something looking like it on shromeries photos.


contam #3 metabolites this early in colonisation usually means bacteria right?


contam #4 this is definitely not cube mycelium. Kinda looks like pic #1 without the discolouration. Maybe spores haven't matured yet?



Thank you.


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Invisiblemicro
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Registered: 05/09/03
Posts: 7,532
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Re: Help me pin the tail on the contaminated donkey. [Re: Supalemonhaze]
    #22480109 - 11/05/15 08:41 AM (8 years, 2 months ago)

For #1, cobweb (Dactylum spp.) where? A good part of the picture is out of focus and it is hard to see in detail so I might be missing it.

For #2, it is also out of focus and I have no idea what that is... If that is supposed to be a jar, where is the substrate? If you are referring to the flat layer of grey stuff present in the area that lacks the white mycelium it could be bacteria. I can't tell if that is media though, such as some type of agar.

For #3, no, secretions aren't indicative of bacteria. They can be in response to bacteria or another type of competitor, however it is very common to see visible secretions and can be caused by a number of things.

For #4, I'll go by what you say -- I can't tell with this one since there is only a tiny dot of colonization.


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InvisibleSupalemonhaze
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Help me pin the tail on the contaminated donkey. [Re: micro]
    #22480290 - 11/05/15 09:22 AM (8 years, 2 months ago)





Those are pics one and 2 again. Pic #4 seems to have the same contam as pic #1 guessing its cobweb but I don't know much about contams hence the post( I like to know all I can about my contams). Its starting to turn grey my guess is its getting mature enough to sporulate. Pic #2 ive honestly got no idea what kind of contam gets that colour. Its super hard to spot until it turns yellow because its mycelium is very white and grows very similarly to cube mycelium.


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OfflinePsilocyBen17
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Re: Help me pin the tail on the contaminated donkey. [Re: Supalemonhaze]
    #22480916 - 11/05/15 12:15 PM (8 years, 2 months ago)

I spy with my little eye something that is green. Your agar looks OK though. just clean it up on another plate. Which species is this?


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Help me pin the tail on the contaminated donkey. [Re: PsilocyBen17]
    #22481076 - 11/05/15 12:57 PM (8 years, 2 months ago)

Yes I already transferred it but I wish to know what that mycelium with the yellow centre is before I dispose of it.

Jars are penis envy agar is mexico dutch king


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OfflinePsilocyBen17
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Re: Help me pin the tail on the contaminated donkey. [Re: Supalemonhaze]
    #22481878 - 11/05/15 04:42 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
Well I too inoculate in open air even though its one of the worst ways to do it. ( my SAB sucks) .




Here is your problem. Found this in the other thread.


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Help me pin the tail on the contaminated donkey. [Re: PsilocyBen17]
    #22482939 - 11/05/15 08:25 PM (8 years, 2 months ago)

Yea well im not really concerned and ill show you why


These are the jars that are doing just fine.


And these are the ones that are contaminated. Its worth noting that the quart one is from a previous grow hence why its so overly colonised. One is an LC one is an agar jar and the one with metabolites will probably survive. But lets say for the sake of argument it doesn't I would have lost 2 to mold which ive been told is probably caused by shitty tyvek. And 1 lost to bacteria.


So you can see why I don't think of this as a "problem". I merely posted here to ID my contams to learn before I chuck them. I thank you for wanting to help but I am doing fine considering I do not have a big enough SAB to use my LC in. Now if you were to be able to ID that yellow mycelium contam I would be eternally grateful though lmao. I am tired of looking at old threads trying to spot it


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