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InvisibleSupalemonhaze
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Strange media plate.
    #22479344 - 11/05/15 03:01 AM (8 years, 2 months ago)

What do you peeps think?



This plate was weird from day one. First week or so you could see something was growing but it was completely see through while the other transfers made the same day grew normally. It does seem isolated so maybe genetics? The yellowish tint is what's making me most curious.


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Invisibleblindingleaf
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Re: Strange media plate. [Re: Supalemonhaze]
    #22479461 - 11/05/15 04:35 AM (8 years, 2 months ago)

ur media might be too strong if that date says 10/21 and that is how much its grown out in 14 days??


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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: blindingleaf]
    #22479659 - 11/05/15 06:34 AM (8 years, 2 months ago)

All transfers made that day are all somewhat the same size.



I bought my agar from a sponsor its already premixed. Recipe is:

24 g agar-agar
20 g malt extract
2 g dry yeast
1 g peptone
Aminoglycoside antibiotics

Edit: back on subject you can see how different said plate looks when compared to the others. All 3 transfers were taken from the same plate.


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Invisibleblindingleaf
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Re: Strange media plate. [Re: Supalemonhaze]
    #22479664 - 11/05/15 06:40 AM (8 years, 2 months ago)

hmmm…..weird.  maybe the antibiotic is causing weird growth pattern?

that recipe is for 1000ml water, so if u used 500ml for one sleeve, it will be really strong.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: blindingleaf]
    #22479673 - 11/05/15 06:44 AM (8 years, 2 months ago)

I actually put a little more water than it says to per reccomendation from someone on this site. Also someone said those kind of antibiotics suck. I still get  bacteria every now and again so I guess that's true.


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InvisiblebodhisattaMDiscordReddit
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Re: Strange media plate. [Re: Supalemonhaze]
    #22479687 - 11/05/15 06:50 AM (8 years, 2 months ago)

The concentric rings are concerning. Unless daily conditions in incubation swing crazily its probably unwanted mold doing that


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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: bodhisatta]
    #22479765 - 11/05/15 07:26 AM (8 years, 2 months ago)

I though the rings are normal. :facepalm3:

Yea they are in incubation at 27c but since its in celcius it will only switch back on when it goes down to 25.9 and switch of at 27.9. So thats like a 4degree farenheit fluctuation happenning every few minutes. Also temp goes even less when its time to shake grains/check up on plates. I check plates for contams daily but that takes less than 5 minutes. The most temp loss happens when I inspect and then shake grain jars.

Edit: I know everyone says not to incubate plates/jars but im an impatient SOB. Always change my mind when I stop using the mat. Unless of course it is being counterproductive then I will chuck that shit in the garbage


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InvisibleFungus Mountain
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Re: Strange media plate. [Re: Supalemonhaze]
    #22479840 - 11/05/15 07:43 AM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
Unless of course it is being counterproductive then I will chuck that shit in the garbage





Just chuck that fucker and save some headaches!


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“Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: Fungus Mountain]
    #22479867 - 11/05/15 07:48 AM (8 years, 2 months ago)

But is it actually being counterproductive? What do the rings mean? Im only apprehensive because blindingleaf said that they are growing very slow. I imagine they will be even slower without it.

Also temp in my room is usually in mid 60's.  Had to buy a small 15watt yellow bulb for the jars that wont fit in the incubator because they were ice cold.


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InvisibleMad Season
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Re: Strange media plate. [Re: Supalemonhaze]
    #22479899 - 11/05/15 07:56 AM (8 years, 2 months ago)

14 days later in 60F.. Sounds a bit slow, but they look alright to me.

I read that the rings are because of the difference in lighting (night, sunny days, and cloudy days).


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Edited by Mad Season (11/05/15 08:04 AM)


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Offlinemushpunx
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Re: Strange media plate. [Re: blindingleaf]
    #22479924 - 11/05/15 08:03 AM (8 years, 2 months ago)

Its definitely nowhere near isolated. I had a plate almost identical to this not too long ago

It changed after a couple transfers. Just keep transferinb amd see what happens


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Invisibleblindingleaf
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Re: Strange media plate. [Re: Mad Season]
    #22479930 - 11/05/15 08:04 AM (8 years, 2 months ago)

in 14 days, they should be totally colonized if its cubes and standard amount of nutrients IME.  if its cooler, then that would explain how slow it is, but i still feel like it would be very close to the edge of that plate.

the rings can be caused by temps and light. 

antibiotics can make things look weird.  pan cyan is on the left is antibiotic MEA
this started out just like typical irregular/symmetrical growth u'd expect from any MS transfer, then stalled, and starting making those satellites


pans are usually much lighter


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: Mad Season]
    #22479933 - 11/05/15 08:04 AM (8 years, 2 months ago)

Maybe its got something to do with the ingredients used in the mixture. Maybe something was not as fresh as it should have been if that makes any sense at all. I will try to make my own agar next as I bought 2 packets of 28g agar flakes. Just gotta find the rest of the ingredients now.

Also when those run out I will try premixed agar from a local lab supply warehouse if the price isn't too bad. Just found out about them. Felt like a kid in a candy shop. I feel like they are a bit on the expensive side although im not entirely sure yet. Tehy got a lot of stuff that interest me so im bound to find out.


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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: blindingleaf]
    #22479965 - 11/05/15 08:09 AM (8 years, 2 months ago)

Quote:

mushpunx said:
Its definitely nowhere near isolated. I had a plate almost identical to this not too long ago

It changed after a couple transfers. Just keep transferinb amd see what happens




Dang I thought I was almost there. I did an lc out of the one with a piece missing. Oh well.


Quote:

blindingleaf said:
in 14 days, they should be totally colonized if its cubes and standard amount of nutrients IME.  if its cooler, then that would explain how slow it is, but i still feel like it would be very close to the edge of that plate.

the rings can be caused by temps and light. 

antibiotics can make things look weird.  pan cyan is on the left is antibiotic MEA
this started out just like typical irregular/symmetrical growth u'd expect from any MS transfer, then stalled, and starting making those satellites


pans are usually much lighter





I honestly dont know why all my agar takes this long to colonise. I always thought it was normal since im new to all cultivation in general. As I said ill try to make some homemade agar and compare them to the premixed one. Maybe they used inferior ingredients or something.

Edit: Mad, its 2 weeks at 80f not 60. Which makes it even slower I guess.


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InvisibleMad Season
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Re: Strange media plate. [Re: Supalemonhaze]
    #22480002 - 11/05/15 08:14 AM (8 years, 2 months ago)

Idk its agar. They won't have thermogenesis like jars and substrates. Lower temps actually are much lower with agar. RR says for agar he found 75-81 is perfect, and for jars you must lower that to like 70-75.

60F is slow on jars which have thermogenesis. I have no idea how it'll effect dishes.. BL might have a better knowledge on that because I can't say I've done agar that low.

Edit: 80?? Jesus yeah that sounds ridiculously slow...


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (11/05/15 08:16 AM)


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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: Mad Season]
    #22480031 - 11/05/15 08:20 AM (8 years, 2 months ago)

Just bought LME online I guess ill find out if its crappy agar mixture in a few weeks. Should be here by the 15th.

Edit: sounds like im not chucking away my heating mat yet then lol.


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Invisibleblindingleaf
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Re: Strange media plate. [Re: Supalemonhaze]
    #22480098 - 11/05/15 08:39 AM (8 years, 2 months ago)

i used the mycosupply pre mix MEA for a few months when i started.
any vendor pre mix should be fine.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: blindingleaf]
    #22480303 - 11/05/15 09:25 AM (8 years, 2 months ago)

I find it weird that they would be selling bad agar ( if there even is auch a thing). I imagine they use it as well to grow/store cultures. Im just so lost as to why its growing slow as everyone said that the only thing I can think of is the agar. Guess ill get my answer in the near future


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InvisiblebodhisattaMDiscordReddit
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Re: Strange media plate. [Re: Supalemonhaze]
    #22480306 - 11/05/15 09:27 AM (8 years, 2 months ago)

27C is super hot try 20-21C


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InvisibleSupalemonhaze
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Re: Strange media plate. [Re: bodhisatta]
    #22480317 - 11/05/15 09:31 AM (8 years, 2 months ago)

Ok. Lower temp might actually help with the drastic fluctuations so theres that. 21 it is then. Lets see how it goes. Hopefully colonisation won't be significantly slower


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