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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
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Re: Gettin back into it... [Re: Pupenhause]
#22491037 - 11/07/15 02:05 PM (8 years, 2 months ago) |
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standard time for 15psi is 90 minutes, I'd try >2 hours.
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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
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Last seen: 15 days, 8 hours
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My thoughts. There's all kinds of mixed reviews on here. 10psi for 2.5 hours probably wouldn't be a bad idea. We shall see...
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Pupenhause
Toughguy


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Re: Gettin back into it... [Re: Pupenhause]
#22491798 - 11/07/15 04:46 PM (8 years, 2 months ago) |
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I hope it all works though. I promised my wife this will be the last time, whether it works or not. So I'm all in here
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PsilocyBen17
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Re: Gettin back into it... [Re: Pupenhause]
#22492547 - 11/07/15 07:13 PM (8 years, 2 months ago) |
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There is no such thing as overkill when it comes to PCing. I used to think my grain would burn or dry out, but as long as there is water in the bottom of the PC during the cycle you are fine.
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FreeWorldOrder


Registered: 12/24/13
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Loc: Indiana, USA
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Re: Gettin back into it... [Re: crackbaby]
#22492559 - 11/07/15 07:17 PM (8 years, 2 months ago) |
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Quote:
crackbaby said: welp, i considered boiling the ziploc bag to sterilize it before dumping the cake into it, but read a post by roger rabbit where he described the relative sterility of a new ziploc, and that he's had successful cultivations inside them (btw, just to be clear the cake is crumbled inside the sealed bag by crushing it with fingers outside da bag ). I lysoled the whole room before doing the transfer, and flame sterilized the spoon used for scooping. Like i said, it worked fine for me, and with quart rye jars that had been sitting for 2 weeks (after failing to colonize from syringe inocculation)
It's not the zip-lock that would be the problem if it is new. It is the fact that the dry verm layer acts as a filter and is probably full of contams. There is a super high probability of transferring said contams directly into your sterile grains...
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Quote:
FreeWorldOrder said:
Quote:
crackbaby said: welp, i considered boiling the ziploc bag to sterilize it before dumping the cake into it, but read a post by roger rabbit where he described the relative sterility of a new ziploc, and that he's had successful cultivations inside them (btw, just to be clear the cake is crumbled inside the sealed bag by crushing it with fingers outside da bag ). I lysoled the whole room before doing the transfer, and flame sterilized the spoon used for scooping. Like i said, it worked fine for me, and with quart rye jars that had been sitting for 2 weeks (after failing to colonize from syringe inocculation)
It's not the zip-lock that would be the problem if it is new. It is the fact that the dry verm layer acts as a filter and is probably full of contams. There is a super high probability of transferring said contams directly into your sterile grains... 
Quote:
FreeWorldOrder said:
Quote:
crackbaby said: welp, i considered boiling the ziploc bag to sterilize it before dumping the cake into it, but read a post by roger rabbit where he described the relative sterility of a new ziploc, and that he's had successful cultivations inside them (btw, just to be clear the cake is crumbled inside the sealed bag by crushing it with fingers outside da bag ). I lysoled the whole room before doing the transfer, and flame sterilized the spoon used for scooping. Like i said, it worked fine for me, and with quart rye jars that had been sitting for 2 weeks (after failing to colonize from syringe inocculation)
It's not the zip-lock that would be the problem if it is new. It is the fact that the dry verm layer acts as a filter and is probably full of contams. There is a super high probability of transferring said contams directly into your sterile grains... 
Earlier in the thread I mentioned that i used a sterilized spoon to scrape da layer of vermiculite from the top of the pf jar (just to be clear, this was thrown into a separate container (aka garbage bag) )
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Gettin back into it... [Re: crackbaby]
#22493217 - 11/07/15 09:48 PM (8 years, 2 months ago) |
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Yea, no. Even if you ran the spoon thru a PC cycle (any other way is not sterilized) and scrape the verm layer off it will be no where clean enough to go into sterile grains.
The only way you would pull this off would be if you skipped the dry verm layer and used a grain jar GE filter. Since you cant break up a PF cake in the jar like rye you would have to wipe it down with ISO and hopefully pop it into a sterile zip lock and break up up by hand and then pour inti grain jars. All this would have to be done following sterile tek and in a still air box.
It would be quite cumbersome and risky, not to mention pointless if you have a PC and therefore the means for grains
--------------------
 Amateur Mycologists United AMU Q&A
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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
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Re: Gettin back into it... [Re: mushpunx]
#22493303 - 11/07/15 10:10 PM (8 years, 2 months ago) |
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Quote:
mushpunx said: Yea, no. Even if you ran the spoon thru a PC cycle (any other way is not sterilized) and scrape the verm layer off it will be no where clean enough to go into sterile grains.
The only way you would pull this off would be if you skipped the dry verm layer and used a grain jar GE filter. Since you cant break up a PF cake in the jar like rye you would have to wipe it down with ISO and hopefully pop it into a sterile zip lock and break up up by hand and then pour inti grain jars. All this would have to be done following sterile tek and in a still air box.
It would be quite cumbersome and risky, not to mention pointless if you have a PC and therefore the means for grains
So after my brf jars are colonized it'll be risky to put it into a wbs jar, even if I pressure cook it?
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mushpunx
Fungus Punk



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Re: Gettin back into it... [Re: Pupenhause]
#22493807 - 11/08/15 01:03 AM (8 years, 2 months ago) |
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Yea absoluetly. Especially of you used a dry verm layer no way would. t be clean enough.
If you can do grains then you should inoculate agar and use a clean culture to inoculate your grains. Thats a clean enough grain master for G2G. Agar is easy. Check out Pasty Plates to start
--------------------
 Amateur Mycologists United AMU Q&A
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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
Loc: over here
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Re: Gettin back into it... [Re: mushpunx]
#22494718 - 11/08/15 08:42 AM (8 years, 2 months ago) |
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Well my brf jars are ready to go. My plan was to use wbs so I'll use at least one jar n take the risk. If it doesn't work, oh well.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Gettin back into it... [Re: Pupenhause]
#22494750 - 11/08/15 08:49 AM (8 years, 2 months ago) |
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Re: Gettin back into it... [Re: mushpunx]
#22495243 - 11/08/15 11:20 AM (8 years, 2 months ago) |
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Quote:
mushpunx said: Yea, no. Even if you ran the spoon thru a PC cycle (any other way is not sterilized) and scrape the verm layer off it will be no where clean enough to go into sterile grains.
The only way you would pull this off would be if you skipped the dry verm layer and used a grain jar GE filter. Since you cant break up a PF cake in the jar like rye you would have to wipe it down with ISO and hopefully pop it into a sterile zip lock and break up up by hand and then pour inti grain jars. All this would have to be done following sterile tek and in a still air box.
It would be quite cumbersome and risky, not to mention pointless if you have a PC and therefore the means for grains
All i can say is that it worked for me, and i'm currently getting my first flush from the cased rye jars that were knocked up from the pf cake...And this was done without a still air box. The spoon was flame sterilized with denatured alcohol btw, and the top layer of vermiculite was thoroughly scraped off before popping the cake into the bag. The results speak for themselves, and i think this is a good way to knock up grain jars if it's not convenient to learn the whole agar tek
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Pupenhause
Toughguy


Registered: 09/09/04
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Re: Gettin back into it... [Re: crackbaby]
#22495248 - 11/08/15 11:22 AM (8 years, 2 months ago) |
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I plan on using a still air box. I assume all liabilities though.
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crackbaby
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Registered: 08/31/15
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Re: Gettin back into it... [Re: Pupenhause]
#22495275 - 11/08/15 11:30 AM (8 years, 2 months ago) |
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good luck mang
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mushpunx
Fungus Punk



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Re: Gettin back into it... [Re: crackbaby]
#22496308 - 11/08/15 03:43 PM (8 years, 2 months ago) |
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Not to be a dick but dumb luck man. I don't doubt it could work but it's not very repeatable, scraping dry verm layer and G2G cakes in open air
Why G2G that at all? Why not spawn those cakes to bulk substrate instead much better chance of good sucess
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 Amateur Mycologists United AMU Q&A
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Pupenhause
Toughguy


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Re: Gettin back into it... [Re: mushpunx]
#22496493 - 11/08/15 04:22 PM (8 years, 2 months ago) |
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Well that was in the back of my mind, as I've done it before with 100%.success, but I was under the impression th wbs would have more nutrients, therefore producing better yields and potentcy?
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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
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Re: Gettin back into it... [Re: Pupenhause]
#22496497 - 11/08/15 04:22 PM (8 years, 2 months ago) |
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Also, even in a still air box you're saying it's a good potential for failure?
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crackbaby
shitpost aficionado



Registered: 08/31/15
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Re: Gettin back into it... [Re: Pupenhause]
#22496591 - 11/08/15 04:39 PM (8 years, 2 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/20410651#20410651
Here's a link to a thread that might be helpful regarding doing g2g with pf cakes.
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mushpunx
Fungus Punk



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Re: Gettin back into it... [Re: Pupenhause]
#22496911 - 11/08/15 05:36 PM (8 years, 2 months ago) |
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Quote:
Pupenhause said: Also, even in a stinll air box you're saying it's a good potential for failure?
Im saying most of us have a hard enough time with G2G from sterile grain masters inoculated with clean agar wedges
There isn't even any point to use a still air box if you are scraping off a nasty dry verm layer full of contaminants. Might as well do that in open air its such a roll of the dice. That is the biggest problem.
If you skip the dry verm layer and use a GE filter lid instead its difficult but a little more plausible. Still the trouble is that since you cant crumble up a PF cake by shaking the jar like you do with a grain jar. Popping the cake into the zip lock without knocking any contams from the outside of the jar in too is the next biggest problem.
In G2G even if you wipe the master jar down with ISO thoroughly if you move any part of the outside of the jar over the top of the reciving jar you can knock contams in.
--------------------
 Amateur Mycologists United AMU Q&A
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