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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
Loc: over here
Last seen: 15 days, 10 hours
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Re: Gettin back into it... [Re: cronicr]
#22486338 - 11/06/15 02:14 PM (8 years, 2 months ago) |
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That's true, bird seed is cheap and I have quite a bit of spore solution.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Gettin back into it... [Re: Pupenhause]
#22486343 - 11/06/15 02:15 PM (8 years, 2 months ago) |
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Exactly and best of luck!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Re: Gettin back into it... [Re: cronicr]
#22486741 - 11/06/15 03:31 PM (8 years, 2 months ago) |
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i recently followed Roger Rabbit's rye tek to a T, and used a spore syringe to inoculate, but after the jars showed no signs of colonization (or contamination)for 2 weeks i decided to crumble up one of my half pint pf cakes in a relatively sterile zip loc bag, and do grain to grain transfer this way, and it worked nicely...One pf cake can be used to knock up 5 quart jars, and the colonization was pretty quick. I'll never use a syringe to inoculate quart rye jars again.
Btw, i initially used 3 ml of spore solution for each quart jar, and the syringe used was good since it worked on some of the pf jars i used it for. Also, i waited plenty of time after pressure cooking before inoculating, so the lack of colonizing wasn't due to the rye being too hot for the spores
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sweetpea

Registered: 12/29/13
Posts: 327
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Edited by sweetpea (04/19/16 11:35 AM)
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Re: Gettin back into it... [Re: sweetpea]
#22486878 - 11/06/15 03:59 PM (8 years, 2 months ago) |
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yup...and the incubation temp was around 77 (same temp as colonization with the crumbled pf cake)
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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
Loc: over here
Last seen: 15 days, 10 hours
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Re: Gettin back into it... [Re: crackbaby]
#22486938 - 11/06/15 04:13 PM (8 years, 2 months ago) |
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Hm. My syringes are about four years old, never used any that old. They're still good I'd inagaine?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Gettin back into it... [Re: Pupenhause]
#22486967 - 11/06/15 04:18 PM (8 years, 2 months ago) |
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probably, only one way to know
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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sweetpea

Registered: 12/29/13
Posts: 327
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Edited by sweetpea (04/19/16 11:34 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Gettin back into it... [Re: sweetpea]
#22486988 - 11/06/15 04:21 PM (8 years, 2 months ago) |
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Quote:
sweetpea said: Shaking multispore inoculated jars before the spores germinate is likely why you saw no signs of growth, then. You should wait to shake jars when using MS syringes.
agreed
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Re: Gettin back into it... [Re: sweetpea]
#22487020 - 11/06/15 04:29 PM (8 years, 2 months ago) |
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Quote:
sweetpea said: Shaking multispore inoculated jars before the spores have a chance to germinate is likely why you saw no signs of growth, then. You should wait to shake jars when using MS syringes.
Oh snap...yeah i shook the crap out of em right after inoculation. Hopefully that's what the problem was...i think i'll still do the grain to grain transfer with crumbled pf cakes on the next grow tho, since it was so fast, and the agar method seems a little too complex for me at this point
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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
Loc: over here
Last seen: 15 days, 10 hours
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Re: Gettin back into it... [Re: crackbaby]
#22487339 - 11/06/15 05:40 PM (8 years, 2 months ago) |
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Well damn it. I could inoculate some pf jars and grain to grain transfer that, since pf tek is what I know already. So many choices. Regardless of what I do, I will getting some tasty mushrooms.
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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
Loc: over here
Last seen: 15 days, 10 hours
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Re: Gettin back into it... [Re: Pupenhause]
#22487352 - 11/06/15 05:42 PM (8 years, 2 months ago) |
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Agar seems pretty straight forward, but if the pf tek is already a proven success for me, there's no reason for me not to try that first, then mess with agar later.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Gettin back into it... [Re: Pupenhause]
#22487362 - 11/06/15 05:43 PM (8 years, 2 months ago) |
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Look up eatyualive's slurry tek. I think it'll fit your needs perfectly
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Gettin back into it... [Re: azur]
#22487390 - 11/06/15 05:46 PM (8 years, 2 months ago) |
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Quote:
azur said: Look up eatyualive's slurry tek. I think it'll fit your needs perfectly
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Gettin back into it... [Re: Pupenhause]
#22487419 - 11/06/15 05:51 PM (8 years, 2 months ago) |
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Re: Gettin back into it... [Re: Pupenhause]
#22487500 - 11/06/15 06:01 PM (8 years, 2 months ago) |
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Quote:
Pupenhause said: Well damn it. I could inoculate some pf jars and grain to grain transfer that, since pf tek is what I know already. So many choices. Regardless of what I do, I will getting some tasty mushrooms.
What worked for me was scraping the top layer of vermiculite off the top of the pf jar with a sterilized spoon, then dumping the cake out of the jar into a new ziploc bag. Seal bag then crumble cake with your hands until it is in pieces no larger than a penny (makes for lots more inoculation points as opposed to large chunks). Then use sterile spoon to scoop out portions of the crumbled cake into the quart jars and shake well. I used micropore tape to cover the four holes i drilled into the tops of the quart jars.
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FreeWorldOrder

Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 10 hours
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Re: Gettin back into it... [Re: crackbaby]
#22487764 - 11/06/15 06:51 PM (8 years, 2 months ago) |
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....
Or you could use a couple TBS. of agar, 2-teaspoon potatoe flakes, 1/2C water and a drop of Karo or honey and make some plates to inoculate your grains. Cheaper, quicker, takes less space, less materials, and much higher success rate.
I just wanted to stress agar is easy to work with and for noobs to not feel intimated by it. I wish I had started agar way sooner than I did. It's like really easy. Especially PastyPlates...
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Gettin back into it... [Re: crackbaby]
#22487803 - 11/06/15 07:00 PM (8 years, 2 months ago) |
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Quote:
crackbaby said:
Quote:
Pupenhause said: Well damn it. I could inoculate some pf jars and grain to grain transfer that, since pf tek is what I know already. So many choices. Regardless of what I do, I will getting some tasty mushrooms.
What worked for me was scraping the top layer of vermiculite off the top of the pf jar with a sterilized spoon, then dumping the cake out of the jar into a new ziploc bag. Seal bag then crumble cake with your hands until it is in pieces no larger than a penny (makes for lots more inoculation points as opposed to large chunks). Then use sterile spoon to scoop out portions of the crumbled cake into the quart jars and shake well. I used micropore tape to cover the four holes i drilled into the tops of the quart jars.
Even doing this in a SAB I don't forsee a whole hell of a lot of success in your future.
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Q&A US vs. THEM The more I learn, the less I know.
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Re: Gettin back into it... [Re: LocN9ne]
#22487847 - 11/06/15 07:13 PM (8 years, 2 months ago) |
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welp, i considered boiling the ziploc bag to sterilize it before dumping the cake into it, but read a post by roger rabbit where he described the relative sterility of a new ziploc, and that he's had successful cultivations inside them (btw, just to be clear the cake is crumbled inside the sealed bag by crushing it with fingers outside da bag ). I lysoled the whole room before doing the transfer, and flame sterilized the spoon used for scooping. Like i said, it worked fine for me, and with quart rye jars that had been sitting for 2 weeks (after failing to colonize from syringe inocculation)
Edited by crackbaby (11/06/15 07:27 PM)
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Pupenhause
Toughguy


Registered: 09/09/04
Posts: 1,382
Loc: over here
Last seen: 15 days, 10 hours
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Re: Gettin back into it... [Re: crackbaby]
#22491015 - 11/07/15 02:01 PM (8 years, 2 months ago) |
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So I got some stupid fancy pressure cooker, apparently it only goes up to 10psi. Rather than 45 minutes, would an hour be better to make up the difference?
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