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OfflinePupenhause
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Gettin back into it...
    #22478044 - 11/04/15 07:40 PM (8 years, 2 months ago)

After quite a few years I'm gonna give it another go.  I'm going to be using popcorn in quart jars, how much spore solution would be recommended for each jar?  I've searched and can't find the answer to this.


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OfflinePsilocyBen17
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Re: Gettin back into it... [Re: Pupenhause]
    #22478071 - 11/04/15 07:46 PM (8 years, 2 months ago)

I'd suggest using quarter pint jars and doing grain-to-grain transfers. Spore solution in a quart jar won't colonize fast and will leave a huge window for contams.


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Invisiblesweetpea
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. [Re: PsilocyBen17]
    #22478121 - 11/04/15 08:01 PM (8 years, 2 months ago)

.


Edited by sweetpea (11/05/15 06:15 PM)


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InvisibleToadstool5
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Re: Gettin back into it... [Re: sweetpea]
    #22478149 - 11/04/15 08:09 PM (8 years, 2 months ago)

:whathesaid:

I used a ml myself and it worked fine but i made sure my popcorn was very dry after boiling and used pint jars.

If your spore syringe isn't clean it won't matter though. Agar is really useful and easy too.

Pastyplates:
https://www.shroomery.org/forums/showflat.php/Number/19208976#19208976

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https://www.shroomery.org/forums/showflat.php/Number/18430998#18430998


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Invisibleazur
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Re: Gettin back into it... [Re: Pupenhause]
    #22478173 - 11/04/15 08:15 PM (8 years, 2 months ago)

Quote:

Pupenhause said:
After quite a few years I'm gonna give it another go.  I'm going to be using popcorn in quart jars, how much spore solution would be recommended for each jar?  I've searched and can't find the answer to this.



We use agar these days


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OfflinePsilocyBen17
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Re: Gettin back into it... [Re: azur]
    #22478176 - 11/04/15 08:16 PM (8 years, 2 months ago)

:wave: Azur!


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Invisibleazur
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Re: Gettin back into it... [Re: PsilocyBen17]
    #22478186 - 11/04/15 08:18 PM (8 years, 2 months ago)



--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineiSmkGrnBud
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Re: Gettin back into it... [Re: azur]
    #22478239 - 11/04/15 08:32 PM (8 years, 2 months ago)

Quote:

azur said:
We use agar these days




:whathesaid:

:youthemandawg:


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OfflineFreeWorldOrder
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Re: Gettin back into it... [Re: iSmkGrnBud]
    #22481414 - 11/05/15 02:48 PM (8 years, 2 months ago)

Definitely want to use agar with grains for best results.

Spore solution can work, but it takes forever and a lot of times will contaminate before colonizing and sometimes stall out.

Like mentioned above using smaller jars would help if you are determined to use the spore solution to inoculate. 

Agar is just so much easier and more reliable.

Doing some transfers tonite actually.


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Offlinemushpunx
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Re: Gettin back into it... [Re: FreeWorldOrder]
    #22482225 - 11/05/15 06:02 PM (8 years, 2 months ago)

Yea never put spores to grain directly. Its icky.


And you might want to pick a better grain, rye or wild bird seed


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OfflinePupenhause
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Re: Gettin back into it... [Re: mushpunx]
    #22482380 - 11/05/15 06:37 PM (8 years, 2 months ago)

Thanks for the input everyone.  Another grain is no problem, but why choose another one over popcorn?


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OfflinePupenhause
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Re: Gettin back into it... [Re: Pupenhause]
    #22482696 - 11/05/15 07:27 PM (8 years, 2 months ago)

One of the wild bird seed teks I looked at uses a spore solution...


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Offlinemushpunx
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Re: Gettin back into it... [Re: Pupenhause]
    #22483714 - 11/05/15 10:45 PM (8 years, 2 months ago)

Quote:

Pupenhause said
One of the wild bird seed teks I looked at uses a spore solution...




Anyone can write a tek dude. Just because it says "tek" doesnt mean its anything but garbage.

Spores to grain will work, it will germinate and colonize and if you're lucky it might even be clean. But that is more of a fluke than anything.

BRF if fairly forgiving and will handle most spore s yringesn. But syringes are rarely clean enough to go to grain.

Most germination plates show at least bacteria that you will have to transfer away from. Bacteria or mold that would otherwise be injected straight into your grains.

Ive inoculated grains w spores and had success. But its just not a good way to innoculste


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OfflinecronicrFacebook
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Re: Gettin back into it... [Re: mushpunx]
    #22483936 - 11/05/15 11:27 PM (8 years, 2 months ago)

2 cc's per qt jar tops, test your syringe first...g2g any healthy jars ya get and look into agar in the meantime, best of luck.


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OfflinePupenhause
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Re: Gettin back into it... [Re: cronicr]
    #22484463 - 11/06/15 05:14 AM (8 years, 2 months ago)

Well I'm not taking any shortcuts, so my best route is going to be agar.    So when the agar is ready is 1cc of solution sufficient?  And when imnready to inoculate my grain jars, how much do I put in there?  Sorry for all the questions, I'm not finding this stuff.


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Offlinemushpunx
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Re: Gettin back into it... [Re: Pupenhause]
    #22484472 - 11/06/15 05:23 AM (8 years, 2 months ago)

Quote:

Pupenhause said:
Well I'm not taking andripped tcuts, so my best route is going to be agar.    So when the agar is ready is 1cc of solution sufficient?  And when imnready to inoculate my grain jars, how much do I put in there?  Sorry for all the questions, I'm not finding this stuff.




1cc would be way too much. Less than 1 drop would be best, dripped onto a sterile swab and swiped on the plate.

A dime size wedge of agar is enough but we all have our preferences. I use more


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OfflineDrCrumbs
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Re: Gettin back into it... [Re: mushpunx]
    #22484909 - 11/06/15 08:37 AM (8 years, 2 months ago)

Yeah you want growth that grows away from the inoculation point, otherwise it's just all mixed together. So if you puddle the plates it wont have clean surface to grow onto.


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OfflinePupenhause
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Re: Gettin back into it... [Re: DrCrumbs]
    #22486270 - 11/06/15 01:56 PM (8 years, 2 months ago)

So what I gathered here is: get some agar, use a sterile swab with a little bit of spore solution on it, swipe my agar.  Wait for said agar to colonize, break off chunks of the agar, put in my grain jars.  Shake grain jars til fully colonized.  Make a casing.  Wait a few short weeks, and  I will enjoy tasty mushrooms.  Correct?


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Offlinemushpunx
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Re: Gettin back into it... [Re: Pupenhause]
    #22486291 - 11/06/15 02:01 PM (8 years, 2 months ago)

e]Pupenhause said:
So what I gathered here is: get some agar, use a sterile swab with a little bit of spore solution on it, swipe my agar.  Wait for said agar to colonize, break off chunks of the agar, put in my grain jars.  Shake grain jars til fully colonized.  Make a casing.  Wait a few short weeks, and  I will enjoy tasty mushrooms.  Correct?




Close. After germination agar you will have to make transfers to new plates until your culture is clean. You would cut a wedge out to inoculate your grains yes.

A casing is a non nutrient layer placed on top of a colonized substrate. I think what you mean is spawn your grain to a pasturized bulk substrate. Once that is colonized you could case if you want.


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Re: Gettin back into it... [Re: mushpunx]
    #22486320 - 11/06/15 02:10 PM (8 years, 2 months ago)

:whathesaid: and being that ya only need a drop or two i'd give your syringe  a shoit while ya wait, whats the worst that can happen


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