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cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
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Pretty sure this is a contam (agar). Opinions?
#22477832 - 11/04/15 06:54 PM (8 years, 2 months ago) |
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Just wanted to get a second opinion on this before I toss it. Been away from myco for a while and only have limited experience anyway. Not really sure what this is, but it really doesn't look like myc to me at all, it's neither ropey or fluffy, but pretty much solid looking. I couldn't seem to find anything about white contams during a search, except for some mentions of bacteria. Is that what you guys think is going on here? Or is this some unusual looking myc?
 Sorry for the pic not being the best. The stuff in the foreground on the walls does not look IRL like it does in the pic. It's just some splashed agar that is the same color as the rest (green), but is reflecting the light weird.
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree. 'Which road do I take?' she asked. 'Where do you want to go?' was his response. 'I don't know', Alice answered. 'Then', said the cat, 'it doesn't matter.'
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PsilocyBen17
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Registered: 10/20/13
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Re: Pretty sure this is a contam (agar). Opinions? [Re: cowsRmeat]
#22477845 - 11/04/15 06:57 PM (8 years, 2 months ago) |
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Yep. Nastly. Clean that up on another plate if you absolutely need to. if not toss it.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Pretty sure this is a contam (agar). Opinions? [Re: PsilocyBen17]
#22477855 - 11/04/15 07:00 PM (8 years, 2 months ago) |
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I would toss it.
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Q&A US vs. THEM The more I learn, the less I know.
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cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
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Re: Pretty sure this is a contam (agar). Opinions? [Re: PsilocyBen17]
#22478041 - 11/04/15 07:40 PM (8 years, 2 months ago) |
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Quote:
PsilocyBen17 said: Yep. Nastly. Clean that up on another plate if you absolutely need to. if not toss it.
Clean it up, you say? Do you see any signs of myc on there?
Thank you guys for the input, too.
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree. 'Which road do I take?' she asked. 'Where do you want to go?' was his response. 'I don't know', Alice answered. 'Then', said the cat, 'it doesn't matter.'
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PsilocyBen17
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Re: Pretty sure this is a contam (agar). Opinions? [Re: cowsRmeat]
#22478060 - 11/04/15 07:44 PM (8 years, 2 months ago) |
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Well, yah man, if you put myc on there then ya theres defiantly myc on there...
You can clean it up if you want but IMO its not worth the trouble.
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cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
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Re: Pretty sure this is a contam (agar). Opinions? [Re: PsilocyBen17]
#22478079 - 11/04/15 07:49 PM (8 years, 2 months ago) |
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That's what I figured. This was the first transfer from a spore germ attempt that looked just like this. I'll just have to try again.
But for reference, do you have any idea what this stuff is? Bacteria? Something else?
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree. 'Which road do I take?' she asked. 'Where do you want to go?' was his response. 'I don't know', Alice answered. 'Then', said the cat, 'it doesn't matter.'
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PsilocyBen17
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Re: Pretty sure this is a contam (agar). Opinions? [Re: cowsRmeat]
#22478090 - 11/04/15 07:51 PM (8 years, 2 months ago) |
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the yellow is bacteria. the green is trich. If you are cloning from a wild fruit you should do atleast 7 transfers.
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cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
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Re: Pretty sure this is a contam (agar). Opinions? [Re: PsilocyBen17]
#22478119 - 11/04/15 08:01 PM (8 years, 2 months ago) |
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Preesh. No, it's not from a wild one this time. It's a print I got through a trade. I'm blaming my SAB technique from being out of practice on this one. Who knows. Also, as I was thinking back on it, I'm using Pasty plates here, and I realized I forgot to add any honey or karo syrup when mixing up the agar, so IDK if that would affect the spores from surviving after germination on a non-nutritious plate...?
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree. 'Which road do I take?' she asked. 'Where do you want to go?' was his response. 'I don't know', Alice answered. 'Then', said the cat, 'it doesn't matter.'
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PsilocyBen17
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Re: Pretty sure this is a contam (agar). Opinions? [Re: cowsRmeat]
#22478170 - 11/04/15 08:14 PM (8 years, 2 months ago) |
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It defiantly would. The whole idea behind an agar plate is for it to outcompete contams. You gotta keep the myc moving. Its not going to grow without a nutrient.
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cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
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Re: Pretty sure this is a contam (agar). Opinions? [Re: PsilocyBen17]
#22478195 - 11/04/15 08:20 PM (8 years, 2 months ago) |
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 Figured. I've already started more plates to get things rolling. The plate in the pic and the other plates made at the same time have now been dumped.
Thanks for the help!
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree. 'Which road do I take?' she asked. 'Where do you want to go?' was his response. 'I don't know', Alice answered. 'Then', said the cat, 'it doesn't matter.'
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FreeWorldOrder

Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
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Re: Pretty sure this is a contam (agar). Opinions? [Re: cowsRmeat]
#22481404 - 11/05/15 02:44 PM (8 years, 2 months ago) |
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Pretty bad. I would start over if possible. If that's the only culture or spores you have then you could try to clean it up....
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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PsilocyBen17
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Re: Pretty sure this is a contam (agar). Opinions? [Re: FreeWorldOrder]
#22481848 - 11/05/15 04:37 PM (8 years, 2 months ago) |
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if you ever need to clean up a plate that is that nasty I might try the "agar sandwich" method Stamets describes in GGMM.
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cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
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Re: Pretty sure this is a contam (agar). Opinions? [Re: PsilocyBen17]
#22482568 - 11/05/15 07:05 PM (8 years, 2 months ago) |
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Quote:
FreeWorldOrder said: Pretty bad. I would start over if possible. If that's the only culture or spores you have then you could try to clean it up....
It's gone now. Hopefully this next try will give me some better results. I just needed to brush up on technique, I guess...
Quote:
PsilocyBen17 said: if you ever need to clean up a plate that is that nasty I might try the "agar sandwich" method Stamets describes in GGMM.
Noted, thank you!
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree. 'Which road do I take?' she asked. 'Where do you want to go?' was his response. 'I don't know', Alice answered. 'Then', said the cat, 'it doesn't matter.'
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