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Offlinemushpunx
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Innoculating gallon jar with agar
    #22474984 - 11/04/15 08:32 AM (8 years, 2 months ago)

Hey all

Any problems just slicing up a plate and dropping the wedges into a gallon jar of spawn, instead of G2G?

Have some of these, and nice big plastic autoclavable lids that I bought one time by accident.

A gallon of spawn is enough for one of my tubs, I might as well use these and if I can get away w A2G all the better

Thanks

-Mushpunx


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InvisibleMr. Alien
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Re: Innoculating gallon jar with agar [Re: mushpunx]
    #22475033 - 11/04/15 08:52 AM (8 years, 2 months ago)

a2g is more safe than g2g IMO. i don't see any inconvenient.


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Offlinemushpunx
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Re: Innoculating gallon jar with agar [Re: Mr. Alien]
    #22475100 - 11/04/15 09:13 AM (8 years, 2 months ago)

Quote:

Mr. Alien said:
a2g is more safe than g2g IMO. i don't see any inconvenient.





Well that's what Im saying lately Ive just been going A2G, one plate to 4 or 5 quarts. Using this for my main grows while I practice getting my G2G innoculations better

But its even less exposure if its cool to inoculate these gallon jars via agar,  pull the jar out of sterilizer in front of the hood, drop agar in, boom....


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Edited by mushpunx (11/04/15 09:14 AM)


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OfflineDrCrumbs
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Re: Innoculating gallon jar with agar [Re: mushpunx]
    #22475147 - 11/04/15 09:28 AM (8 years, 2 months ago)

I had really good success with gallon bags, did lots of these.

Gallon jars would stall out a lot. Looking back I think it was lack of fae.


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Offlinemushpunx
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Re: Innoculating gallon jar with agar [Re: DrCrumbs]
    #22475217 - 11/04/15 09:51 AM (8 years, 2 months ago)

Quote:

DrCrumbs said:
I had really good success with gallon bags, did lots of these.

Gallon jars would stall out a lot. Looking back I think it was lack of fae.




Yea I use 5 to 6 quarts in spawn bags all the time but I G2G into those.

You wouldn't have any FAE in a jar, just Gas exchange through the filter.

That's the only thing I wanna make sure before I give them a go , that they don't stall out or something from just wedge innoculations. I use half gallons sometimes and those work just fine :shrug:


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InvisibleSupalemonhaze
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Re: Innoculating gallon jar with agar [Re: mushpunx]
    #22475264 - 11/04/15 09:59 AM (8 years, 2 months ago)

Quote:

mushpunx said:
Quote:

DrCrumbs said:
I had really good success with gallon bags, did lots of these.

Gallon jars would stall out a lot. Looking back I think it was lack of fae.




Yea I use 5 to 6 quarts in spawn bags all the time but I G2G into those.

You wouldn't have any FAE in a jar, just Gas exchange through the filter.

That's the only thing I wanna make sure before I give them a go , that they don't stall out or something from just wedge innoculations. I use half gallons sometimes and those work just fine :shrug:




I think GE is what he meant. And it makes sense a gallon jar would have more difficulty exchanging gas than a smaller jar. Just think of how much obsticales are in the way for the gas to go from the bottom to the top. Maybe one needs to shake it more often? I dunno I haven't gone bigger than quart so far and I have a feeling I wont go bigger anytime soon. Bought 130 euro worth of jars and only realised I bought pints when they arrived. Was so pissed. But I guess I can still work with pints for grains so no more spending now. Im broke as broke gets


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OfflineDrCrumbs
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Re: Innoculating gallon jar with agar [Re: Supalemonhaze]
    #22475334 - 11/04/15 10:15 AM (8 years, 2 months ago)

yeah, haha sorry GE.


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InvisibleMad Season
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Re: Innoculating gallon jar with agar [Re: DrCrumbs]
    #22475939 - 11/04/15 10:57 AM (8 years, 2 months ago)

Lots of people colonize gallon jars on their side. It makes it much easier for the bottom to get GE. Just throwing that out there.


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InvisibleLocN9ne
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Re: Innoculating gallon jar with agar [Re: Mad Season]
    #22475961 - 11/04/15 11:03 AM (8 years, 2 months ago)

If you shake the piss out of it I can't think of any reason it wouldn't work... if it ever didn't work, it was probably due to not pressure cooking long enough, or some pansy ass myc.


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InvisibleSupalemonhaze
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Re: Innoculating gallon jar with agar [Re: Mad Season]
    #22476868 - 11/04/15 02:47 PM (8 years, 2 months ago)

Quote:

Mad Season said:
Lots of people colonize gallon jars on their side. It makes it much easier for the bottom to get GE. Just throwing that out there.




Really? How's that? :o I figured it would be the other way round


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InvisibleMad Season
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Re: Innoculating gallon jar with agar [Re: Supalemonhaze]
    #22476900 - 11/04/15 02:56 PM (8 years, 2 months ago)

I guess it's because the hole is in the middle, as well as within the grains. It also helps if you don't pound the jar down hardcore lol.


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Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleSupalemonhaze
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Re: Innoculating gallon jar with agar [Re: Mad Season]
    #22476947 - 11/04/15 03:07 PM (8 years, 2 months ago)

Ohh I just understood the first post. Yea that makes sense. More surface area and less distance from top to bottom.


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InvisibleMr. Alien
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Re: Innoculating gallon jar with agar [Re: Mad Season]
    #22477166 - 11/04/15 04:04 PM (8 years, 2 months ago)

Quote:

Mad Season said:
Lots of people colonize gallon jars on their side. It makes it much easier for the bottom to get GE. Just throwing that out there.




:whathesaid:


Just tilt the jar or completely lying down the jar on their side works. Vertical colonization is just not good with gallon jars.


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InvisibleSupalemonhaze
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Re: Innoculating gallon jar with agar [Re: Mr. Alien]
    #22478939 - 11/04/15 11:35 PM (8 years, 2 months ago)

Nice hood there mate


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OfflinecronicrFacebook
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Re: Innoculating gallon jar with agar [Re: Supalemonhaze]
    #22478981 - 11/04/15 11:45 PM (8 years, 2 months ago)

Pc the grains longer and yep on its side


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OfflineDrCrumbs
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Re: Innoculating gallon jar with agar [Re: cronicr]
    #22480099 - 11/05/15 08:39 AM (8 years, 2 months ago)

Oh nice, yeah they were not on their sides. I PC'ed for 90 mins, that may have also not been enough.

I was looking back through some or my old notes.

ALT#7 actually worked very well like this.

75% (3 outta 4) of cube jars worked, but were very slow at the end. Bags were like 2 weeks and the jars look like they were more like a month or so.

I was really into bags at the time also. So I wasn't giving them much attention.


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InvisibleMad Season
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Re: Innoculating gallon jar with agar [Re: DrCrumbs]
    #22480119 - 11/05/15 08:43 AM (8 years, 2 months ago)

General consensus is 90 minutes in general isn't long enough for grains. I do 2hours minimum with grains.

Isn't it funny when you hear how we get skewed views of things because we didn't do it correctly? :lol:


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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Edited by Mad Season (11/05/15 08:43 AM)


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Offlinemushpunx
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Re: Innoculating gallon jar with agar [Re: Mad Season]
    #22480136 - 11/05/15 08:45 AM (8 years, 2 months ago)

Yea quarts I do 2hrs and anything bigger I go 2.5 to 3.

Now with the sterilizer I run at 25PSI


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