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OfflineAliasbane
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Still Air Box Filter Question
    #22470983 - 11/03/15 12:52 PM (8 years, 2 months ago)

Hello!  So with the still air box I am going to make, gonna try to make it relatively nice.  With the 5" Pipe Fitting for arm holes and such.

I am most likely going to use the bin upside on a 10% bleach soaked Towel.

Do I need to make a single say 1 to 1 1/2 inch hole with some sort of filter material in it/ small pipe chimney?

Just trying to understand it completely.


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Offlinemushpunx
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Re: Still Air Box Filter Question [Re: Aliasbane]
    #22471019 - 11/03/15 01:01 PM (8 years, 2 months ago)

The still air box just keeps the air still as possible, to keep contaminants from settling into any open sterile work.

No need to soak a towel in bleach or any filters. Just a box that is easy to see thru, with arm holes with space around your arms, that's big enough to suit whatever kind of work you are doing comfortably.

  Just spray the walls and air inside lightly with some water and dish soap before you work, let it settle. This helps catch contams that are floating in the air and make them stick.


People do find neat ways of closing off the arm holes, which isn't necessary but can be nice. Like PVC pipe with threaded caps.

Just be sure to make it big enough to be comfortable to work in. Dont wanna be banging jars on the ceiling or letting your work suffer because its cramped


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Edited by mushpunx (11/03/15 01:04 PM)


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OfflineDrCrumbs
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Re: Still Air Box Filter Question [Re: mushpunx]
    #22471040 - 11/03/15 01:07 PM (8 years, 2 months ago)

Yeah a filter will just cause air flow.


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OfflineAliasbane
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Re: Still Air Box Filter Question [Re: DrCrumbs]
    #22471243 - 11/03/15 02:01 PM (8 years, 2 months ago)

Yeah gonna use a 66qt container or may a 100qt one.  But I keep reading stuff and getting advice and things seem to contradict a little if you try to get nice...


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Offlinemushpunx
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Re: Still Air Box Filter Question [Re: Aliasbane]
    #22471329 - 11/03/15 02:28 PM (8 years, 2 months ago)

Go for the 100qt. Itll just make life easier if you get into grain to grain transfers.

But yea, all you ever needfir SAB is tub, 2 armholes, maybe a soapy spraybottle and ISO to wipe your work down with before it goes inside.


And gloves.


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InvisibleToadstool5
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Re: Still Air Box Filter Question [Re: Aliasbane]
    #22471695 - 11/03/15 03:43 PM (8 years, 2 months ago)

No need for an additional hole :thumbup:

Tyvek sleeves, surgical masks, bleach instead of soap water, and HEPA filtration of the room air before starting to prepare the SAB are much better if you are paranoid. They aren't necessary but i like to utilize them myself.

The closeable arm holes with the pipe-fittings are really nice if you want to walk away in the middle of working, but I simply place my bleach soaked cloth over the holes when I walk away :shrug:


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OfflineAliasbane
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Re: Still Air Box Filter Question [Re: Toadstool5]
    #22476042 - 11/04/15 11:25 AM (8 years, 2 months ago)

So on the topic of my SAB.  Would it be a good idea to do it inside some sort of tent setup in my basement?  I have a large 4 person camping tent (Brand New in box).  I could work inside to keep things even more sterile by using the 10% bleach in a spray bottle.


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InvisibleMad Season
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Re: Still Air Box Filter Question [Re: Aliasbane]
    #22476060 - 11/04/15 11:30 AM (8 years, 2 months ago)

Sure.. But being meticulous and paranoid gets tiring.. If you're at all like me, you look for the easiest laziest ways of doing things. All you need is a still room, a big ass box inside that still room, large arm holes, and a spray bottle. You can make due with just those. I know because I have been since may lol


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OfflineiSmkGrnBud
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Re: Still Air Box Filter Question [Re: Mad Season]
    #22476086 - 11/04/15 11:38 AM (8 years, 2 months ago)

:whathesaid:

I would go the easier route mate. As long as you can use a SAB inside a still room with soapy water, you're gonna be fine, there shouldn't be any major issues with contamination. Like Mad said, it saves time and a lot of meticulous work doing it the simple way. :thumbup:


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OfflineAliasbane
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Re: Still Air Box Filter Question [Re: Mad Season]
    #22476089 - 11/04/15 11:39 AM (8 years, 2 months ago)

Quote:

Mad Season said:
Sure.. But being meticulous and paranoid gets tiring.. If you're at all like me, you look for the easiest laziest ways of doing things. All you need is a still room, a big ass box inside that still room, large arm holes, and a spray bottle. You can make due with just those. I know because I have been since may lol




haha i understand, i am fighting my urge to just "DO IT!", and want no points of cantamination, I am rather paranoid, have a cat in the house, live in basement etc...


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InvisibleHigher Love
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Re: Still Air Box Filter Question [Re: Mad Season]
    #22476100 - 11/04/15 11:43 AM (8 years, 2 months ago)

Look at my journal SAB thread. In this box I have had 0 grains contam while inoculation and g2g, 1 in maybe 50 petri contam using a pour tek and I even let my Petri's sit open in the box for an hour to dry then close the lids. Don't over think it. It's about what 7 do in the box, not so much the box


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Offlinemushpunx
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Re: Still Air Box Filter Question [Re: Aliasbane]
    #22476141 - 11/04/15 11:52 AM (8 years, 2 months ago)

Quote:

Aliasbane said:
Quote:

Mad Season said:
Sure.. But being meticulous and paranoid gets tiring.. If you're at all like me, you look for the easiest laziest ways of doing things. All you need is a still room, a big ass box inside that still room, large arm holes, and a spray bottle. You can make due with just those. I know because I have been since may lol




haha i understand, i am fighting my urge to just "DO IT!", and want no points of cantamination, I am rather paranoid, have a cat in the house, live in basement etc...





It comes down to understanding the core concepts and sterile technique. You'll make yourself miserable if you go way overkill.
Use agar to get a nice clean culture for innoculant, and maybe just inoculate your jars agar to grain the first few runs of spawn and then move up to grain to grain transfers later on.

If you go clean culture to agar you will have a very high chance of success


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InvisibleLocN9ne
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Re: Still Air Box Filter Question [Re: mushpunx]
    #22476159 - 11/04/15 11:56 AM (8 years, 2 months ago)

Right... actually using a sab in a tent would be worse than using it in an open room...since any air currents caused by your movement would be deflected into numerous directions because of the walls of the tent... just do it in a room without moving air...like fans or heater vents


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OfflineAliasbane
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Re: Still Air Box Filter Question [Re: mushpunx]
    #22476199 - 11/04/15 12:05 PM (8 years, 2 months ago)







It comes down to understanding the core concepts and sterile technique. You'll make yourself miserable if you go way overkill.
Use agar to get a nice clean culture for inoculant, and maybe just inoculate your jars agar to grain the first few runs of spawn and then move up to grain to grain transfers later on.

If you go clean culture to agar you will have a very high chance of success




okay so going agar to grain for inoculation, you have to open the complete jar lid, do I have to worry ? also it needs a little venting at the top, i could stuff with poly fill right?


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InvisibleHigher Love
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Re: Still Air Box Filter Question [Re: Aliasbane]
    #22476716 - 11/04/15 02:13 PM (8 years, 2 months ago)

Yes u will have to open the lid the whole way. Unless you use the tek for liquid Inoculate. That has given me great success but requires fabrication and an extra syringe.
But don't stress too much man. I've used my still air box for grain inoculations with agar wedges a few times and it turned out fine, just think about ur moves etc.. (sterile technique). Mine even had a piece of super edge-frayed tyvek that I hadn't trimmed so it had to come completely off too, was sketchy but haven't had contams at all.
Prepare many jars and just do it. Wait a few days in between each jar if u have to, success will come greatly thru experimentation.

Edit: yes, u need a vent. Look up some ways, there are many ways to do this that are all successful


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Edited by Higher Love (11/04/15 02:14 PM)


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InvisibleMad Season
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Re: Still Air Box Filter Question [Re: Higher Love]
    #22476758 - 11/04/15 02:24 PM (8 years, 2 months ago)

U can check out my sterile techniques link in my SIG :shrug: it could maybe help.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleGovam
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Re: Still Air Box Filter Question [Re: Mad Season]
    #22479779 - 11/05/15 07:29 AM (8 years, 2 months ago)

If you're using flame sterilization in combination with ISO as a sterilizer....wouldn't it be smart to have a filtered air exchange/exhaust? This thread is kind of scary, talking about spraying ISO in a contained space where people might possibly set up a burner lamp or at the very least use a lighter on a needle.

Just be careful guys, remember that shit is flammable and if your box has no holes the ISO/flammable disinfectant spray is still in the box as vapor.


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OfflineAliasbane
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Re: Still Air Box Filter Question [Re: Mad Season]
    #22479784 - 11/05/15 07:29 AM (8 years, 2 months ago)

Quote:

Mad Season said:
U can check out my sterile techniques link in my SIG :shrug: it could maybe help.




Thanks for the link direction!


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Offlinethebug76
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Re: Still Air Box Filter Question [Re: Govam]
    #22480243 - 11/05/15 09:09 AM (8 years, 2 months ago)

Quote:

Govam said:
If you're using flame sterilization in combination with ISO as a sterilizer....wouldn't it be smart to have a filtered air exchange/exhaust? This thread is kind of scary, talking about spraying ISO in a contained space where people might possibly set up a burner lamp or at the very least use a lighter on a needle.

Just be careful guys, remember that shit is flammable and if your box has no holes the ISO/flammable disinfectant spray is still in the box as vapor.





I keep my alcohol lamp outside my SAB about a foot or so away to the side. Don't flame inside unless you're just usong soap and water.


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InvisibleMad Season
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Re: Still Air Box Filter Question [Re: thebug76]
    #22480247 - 11/05/15 09:11 AM (8 years, 2 months ago)

No just don't flame inside at all.. Flame needs clean air to burn, so it will circulate the air. In that case it'd be a circulating air box..


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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