|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: Constant Contamination [Re: mushpunx]
#22522012 - 11/14/15 11:38 AM (8 years, 2 months ago) |
|
|
I did PF tek, then crumbled and layered with hpoo/CVG in a mini tray setup. I haven't fruited straight cakes tho.
I've just been pretty lucky with the jars by following the tips, I gave earlier, and they've all been MS to PF jars.
Edited by thebug76 (11/14/15 11:41 AM)
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Constant Contamination [Re: thebug76]
#22522261 - 11/14/15 12:40 PM (8 years, 2 months ago) |
|
|
pink mold is dirty inoculant or lack of sterile procedure
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
|
Re: Constant Contamination [Re: redrum]
#22522473 - 11/14/15 01:33 PM (8 years, 2 months ago) |
|
|
Quote:
redrum said: Hey guys,
I tried twice to cultivate following pf tek to a "T". Pink mold is a constant issue.
Washing the jars with antibacterial soap, spray with bleach, and rinse again. Prepare the substrate and in the cooker they go. 15psi for 1hr.
Try PC'ing for 90 minutes.
Quote:
redrum said: Pot cools down for about 6 hours. Prepare room by spraying EVERYTHING down with bleach and lysol, including pressure cooker before opening and letting it sit for 10 min. I even wore rubber gloves and sprayed them. I opened the cooker and sprayed bleach solution inside on tinfoil covered jars. Heat syringe lift foil and inject.
I reheated the lighter after every injection and covered jars again and placed them individually inside zip lock bags. I sprayed the inside of the bags, including the covered jars again with bleach solution and sealed.
I sterilized a big tupperware container and put zip lock bags inside and sprayed again for good measure and closed lid. Put the tupperware inside a black bag and sprayed AGAIN. All goes well for 5 days then I start seeing pink. I don't think there is anything else I can do other then investing in a sterile room...
Any ideas?
You need to build a SAB to do your inoculation work. It's super simple and very effective. I rarely get contams when using proper sterile techniques. Get a 66qt tote or larger and melt two large holes big enough for your hands to fit through on one of the long sides. Additionally, you can attach some tyvek sleeves to the outside.

And check out the first link in my sig.
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: Constant Contamination [Re: mushpunx]
#22525188 - 11/14/15 10:18 PM (8 years, 2 months ago) |
|
|
Quote:
mushpunx said: Then what are the trays in your sig, that's PF tek but in like PP5s? They are gorgeous
Oh yea, thanks by the way. I was really proud of those, especially since it was my first successful grow. Sorry I forgot to say thanks earlier, I was in a hurry getting ready for work. I've got another grow in progress. Still in the jar stage now, but I'm planning 12 - 1qt pp5's instead of 3 like that first grow and they're all gonna be the bigger strains (6 GT and 6 AA+).
-------------------- Bug
Edited by thebug76 (11/14/15 10:19 PM)
|
redrum
Aspiring Mycologist

Registered: 11/03/15
Posts: 169
|
Re: Constant Contamination [Re: thebug76]
#22526782 - 11/15/15 09:19 AM (8 years, 2 months ago) |
|
|
That might might have been part of the problem. There was no air in the syringe for me to shake it to break up the clumps so I pulled back the plunger while the needle was in fire to ensure the air was sterile.
I just remembered I tossed all my jars after being annoyed at the contamination. All I have now is pint size jars. Are they too big for pf tek?
|
redrum
Aspiring Mycologist

Registered: 11/03/15
Posts: 169
|
Re: Constant Contamination [Re: thebug76]
#22526805 - 11/15/15 09:24 AM (8 years, 2 months ago) |
|
|
Quote:
thebug76 said: TIP: Keep the needle pointed up while flaming, don't pull any air into the syringe, make sure it gets red hot, don't wipe it with anything, don't put the foil back on after inoculation.
PF tek is all I've done so far with colonization, but I am yet to see a mold in a jar and I only steam because I don't have a PC. A little bacteria a couple times tho.
Don't put foil back in after inoculation? I'm going to have two tiny open holes in the bottom layers of foil. Won't that put the jars more at risk?
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: Constant Contamination [Re: redrum]
#22526880 - 11/15/15 09:55 AM (8 years, 2 months ago) |
|
|
Quote:
redrum said:
Quote:
thebug76 said: TIP: Keep the needle pointed up while flaming, don't pull any air into the syringe, make sure it gets red hot, don't wipe it with anything, don't put the foil back on after inoculation.
PF tek is all I've done so far with colonization, but I am yet to see a mold in a jar and I only steam because I don't have a PC. A little bacteria a couple times tho.
Don't put foil back in after inoculation? I'm going to have two tiny open holes in the bottom layers of foil. Won't that put the jars more at risk?
When your using PF tek, the dry verm acts as a barrier and leaving tge foil off allows the tops of the jars to dry out. Leaving it on, traps moisture which germinates contams and lets the moisture, as well as the contam, to work its way thru the verm barrier to your sub.
-------------------- Bug
|
redrum
Aspiring Mycologist

Registered: 11/03/15
Posts: 169
|
Re: Constant Contamination [Re: thebug76]
#22527171 - 11/15/15 11:36 AM (8 years, 2 months ago) |
|
|
When your using PF tek, the dry verm acts as a barrier and leaving tge foil off allows the tops of the jars to dry out. Leaving it on, traps moisture which germinates contams and lets the moisture, as well as the contam, to work its way thru the verm barrier to your sub.
Interesting. Every thing I read and watched insists on putting the foil back on. I do see your point and it makes sense. I will leave the top layer off this time and just leave the layer I inoculate through on. Thanks
Once inoculated, can I put them in a plastic container then in a black garbage bag to keep them dark, or just leave them out in open air? Can I use pint size jars and cut them in half once birthed?
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: Constant Contamination [Re: redrum]
#22527275 - 11/15/15 12:05 PM (8 years, 2 months ago) |
|
|
I just put them in a clothes drawer that has a little extra room, but most of the guys on here say just stick them on a shelf. Light doesn't hurt anything and you want room temperature.
-------------------- Bug
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: Constant Contamination [Re: thebug76]
#22527350 - 11/15/15 12:26 PM (8 years, 2 months ago) |
|
|
I wouldn't use anything bigger than 1/2 pint (8 oz) for PF tek, otherwise they tend to stall out. And yes open air.
-------------------- Bug
|
|