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IngSocTHC



Registered: 07/06/05
Posts: 281
Last seen: 7 years, 11 months
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PE agar advice (pic)
#22469136 - 11/03/15 12:02 AM (8 years, 2 months ago) |
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I just found these guys, 3-4 weeks old... Wondering oppinon on what sections to take if any. The rye spawn came from a batch of PE that I let over colonize by a good month. The jar looked iffy (bacteria) and it was all I had left so I went for a hail marry on my first agar attempt. Now I am left with this of my PE any advice?
-------------------- "Music your, music your the key. Talk to who, plese talk to me. Bring the voice of the rastaman communicating to everyone" - Bob Marley
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coneconington
human



Registered: 11/11/12
Posts: 103
Last seen: 4 years, 1 month
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Re: PE agar advice (pic) [Re: IngSocTHC]
#22469190 - 11/03/15 12:24 AM (8 years, 2 months ago) |
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I would take the rhizomorphic sectors coming up from the left of the transfer on the right, if that make sense. Just above the "p" or "o".
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IngSocTHC



Registered: 07/06/05
Posts: 281
Last seen: 7 years, 11 months
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Quote:
coneconington said: I would take the rhizomorphic sectors coming up from the left of the transfer on the right, if that make sense. Just above the "p" or "o".
yup makes sense. only way to know if it fruits is by growing it out? what are the odds of a fruiting from non? also would it be better just to grow some out and take from a cluster?
-------------------- "Music your, music your the key. Talk to who, plese talk to me. Bring the voice of the rastaman communicating to everyone" - Bob Marley
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: PE agar advice (pic) [Re: IngSocTHC]
#22469266 - 11/03/15 01:29 AM (8 years, 2 months ago) |
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Same. No need to limit the diversity too much yet. A single grain will already have less diversity than the whole jar, and by taking that nice region you're limiting it yet again. Grab a clone that looks good from whatever you grow with that.
You could also see if it pins on the plate, transfer the best lookings pin(s) and grow that.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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coneconington
human



Registered: 11/11/12
Posts: 103
Last seen: 4 years, 1 month
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If you're looking for a 'sure fruiter' then I'd agree with machi on letting the plate pin. But from my experience, up until your 4th or so transfer you still have a very varied culture, so the liklihood of having only non fruiting strains is pretty small. I've never had an isolate not produce, but I've also never worked with PE. If it were me, I'd transfer the 2 sectors mentioned above without being too specific, then agar to grain, fruit them and clone the fruit you like most. Or you could keep transferring different sectors to new plates until each plate has a constant growth pattern, apply each plate to some grain and a master slant, grow out each culture. Choose the one you like/performs the best. This way you have the slants to go back to if one proves to be superior.
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