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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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sterility of tape types off the roll
#22469078 - 11/02/15 11:40 PM (8 years, 2 months ago) |
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Hey, I'm working on a sort of mini spawn bag thing, but they have a small set of holes that only needs to be covered after the pc run to prevent air being sucked in while crumbling/shaking the sub. There will be a separate filter patch for GE.
My question is simply what sorts of tape besides micropore are sterile within the roll. For instance the glue side of masking or duct tape. I'm not terribly concerned at all, but the chances of a contam making it in are so small that I cannot simply test.the tape and see if contams show up, since on making it in are so small, I couldn't make a statistically large enough sample to check, or let alone have it outweigh the chances of hidden contams in the inoculant.
Any medical tape would probably be fine, but I'm looking for common household supplies.
Thanks.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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When i first started bags and didn't have a hood, i would inject with LC, and put duct tape over that hole, and it was fine.
i don't think it would survive PC cycle though.
clear packing tape would be good too.
masking tape would get wet.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: sterility of tape types off the roll [Re: blindingleaf]
#22470963 - 11/03/15 12:47 PM (8 years, 2 months ago) |
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I use 1" white masking tape over my injection holes. I wipe a bag with alcohol, lay some tape half way down, inject through the bag, then cover with the tape. Sometimes I double layer it. Don't know if it's needed. Never had a tape related contam. I have recooked bags that didn't grow with the tape on it. The tape survived the 3 hour 23psi pressure cook.
I would assume that most tape is pretty dam clean, if not sterile. Plus the adhesive would probably hold any bad spores that hot mixed up with it
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: sterility of tape types off the roll [Re: tahoe]
#22471200 - 11/03/15 01:50 PM (8 years, 2 months ago) |
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Okay cool. That's about all the info I need I think, and pretty much what I figued. Even if it was dirty, whatever contams were there got smashed into the adhesive when the tape was rolled. It's unfortunate that so much contam fear mongering goes on what stabbing a hole in a bag with a needle and putting tape over it is pretty damn clean.
Thanks so much!
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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