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Offlinejivangilad
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Liquid culture O.K?
    #22465157 - 11/02/15 12:59 AM (8 years, 2 months ago)

First time I do liquid culture, in honey.
Is this O.K. already, can I use it?


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Invisibleazur
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Re: Liquid culture O.K? [Re: jivangilad]
    #22465187 - 11/02/15 01:28 AM (8 years, 2 months ago)

Put a drop on agar and let us know


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Offlinemigraineur
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Re: Liquid culture O.K? [Re: azur]
    #22465233 - 11/02/15 02:02 AM (8 years, 2 months ago)

Inoculate a test jar and see what happens.


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InvisibleSupalemonhaze
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Re: Liquid culture O.K? [Re: migraineur]
    #22465498 - 11/02/15 06:45 AM (8 years, 2 months ago)

Goodluck with it! I guarantee ull fall in love with it if all goes well. Colonisation starts faster and you can make your isolates and spores last so much longer


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Offlinejivangilad
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Re: Liquid culture O.K? [Re: azur]
    #22466397 - 11/02/15 11:57 AM (8 years, 2 months ago)

So I still need to test it.
The fact it has mycelium, doesn't mean  it is not contaminated.


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InvisibleLocN9ne
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Re: Liquid culture O.K? [Re: jivangilad]
    #22466402 - 11/02/15 11:59 AM (8 years, 2 months ago)

Nope...sure dont...that's the drawback of LC is you won't see contamination until you grow it out...put it to agar, if it is in fact clean then :freewilly:


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Offlinejivangilad
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Re: Liquid culture O.K? [Re: LocN9ne]
    #22466631 - 11/02/15 01:15 PM (8 years, 2 months ago)

Quote:

LocN9ne said:
Nope...sure dont...that's the drawback of LC is you won't see contamination until you grow it out



I wonder why. Contamination doesn't grow on liquid culture?


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OfflineFungusOfTheBungus


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Re: Liquid culture O.K? [Re: jivangilad]
    #22467172 - 11/02/15 03:46 PM (8 years, 2 months ago)

Quote:

jivangilad said:
Quote:

LocN9ne said:
Nope...sure dont...that's the drawback of LC is you won't see contamination until you grow it out



I wonder why. Contamination doesn't grow on liquid culture?





Contaminants can most definitely grow in LC. Usually there is so much mushroom mycelium that the contams cannot take over the LC. However, when you suck some up in a syringe and inoculate a jar of substrate then the contams have a nice new playground for themselves and they have a better chance to compete against the mushroom mycelium.

I have an old jar of LC that got accidently left out at room temperature for several months. There appears to be several large spots of trichoderma floating on the top of the liquid. I'm not even going to bother trying to save the jar. In the trash it will go.


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InvisibleSupalemonhaze
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Re: Liquid culture O.K? [Re: FungusOfTheBungus]
    #22468324 - 11/02/15 08:10 PM (8 years, 2 months ago)


Quote:

FungusOfTheBungus said:
Quote:

jivangilad said:
Quote:

LocN9ne said:
Nope...sure dont...that's the drawback of LC is you won't see contamination until you grow it out



I wonder why. Contamination doesn't grow on liquid culture?





Contaminants can most definitely grow in LC. Usually there is so much mushroom mycelium that the contams cannot take over the LC. However, when you suck some up in a syringe and inoculate a jar of substrate then the contams have a nice new playground for themselves and they have a better chance to compete against the mushroom mycelium.

I have an old jar of LC that got accidently left out at room temperature for several months. There appears to be several large spots of trichoderma floating on the top of the liquid. I'm not even going to bother trying to save the jar. In the trash it will go.




I leave all my LC's in room temperature. Some I even leave in front of a yellow bulb for some warmth.


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OfflineFungusOfTheBungus


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Re: Liquid culture O.K? [Re: Supalemonhaze]
    #22468358 - 11/02/15 08:21 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:

Quote:

FungusOfTheBungus said:
Quote:

jivangilad said:
Quote:

LocN9ne said:
Nope...sure dont...that's the drawback of LC is you won't see contamination until you grow it out



I wonder why. Contamination doesn't grow on liquid culture?





Contaminants can most definitely grow in LC. Usually there is so much mushroom mycelium that the contams cannot take over the LC. However, when you suck some up in a syringe and inoculate a jar of substrate then the contams have a nice new playground for themselves and they have a better chance to compete against the mushroom mycelium.

I have an old jar of LC that got accidently left out at room temperature for several months. There appears to be several large spots of trichoderma floating on the top of the liquid. I'm not even going to bother trying to save the jar. In the trash it will go.




I leave all my LC's in room temperature. Some I even leave in front of a yellow bulb for some warmth.




:raisemyglass: You must have some good sterile technique!


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Invisibleenlightened seed
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Re: Liquid culture O.K? [Re: jivangilad]
    #22468432 - 11/02/15 08:37 PM (8 years, 2 months ago)

Quote:

jivangilad said:
First time I do liquid culture, in honey.
Is this O.K. already, can I use it?






can not tell by the pics.  not a whole lot of detail.  try it out.


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Offlinemigraineur
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Re: Liquid culture O.K? [Re: enlightened seed]
    #22468736 - 11/02/15 09:39 PM (8 years, 2 months ago)

When I was using liquid cultures I'd leave my liquid culture jars and syringes in the fridge. They'd be good to use for at least a year. They start going down hill after that.


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InvisibleSupalemonhaze
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Re: Liquid culture O.K? [Re: migraineur]
    #22469213 - 11/03/15 12:45 AM (8 years, 2 months ago)

Well I only worked with 1 culture so far. Got another 2 which are still colonising so I may just have been lucky. I did have a few contam issues with trich but It was impossible to tell where it was coming from because I still didn't have a good enough PC.

I will try them out on agar though when the new ones colonise. You guys just made me curious


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Offlinejivangilad
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Re: Liquid culture O.K? [Re: azur]
    #22469267 - 11/03/15 01:30 AM (8 years, 2 months ago)

Quote:

azur said:
Put a drop on agar and let us know



In this case I wonder why bother doing LC in the first place.
Seems simpler to do an agar culture, and then make a syringe from it.
What am I missing?


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InvisibleMad Season
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Re: Liquid culture O.K? [Re: jivangilad]
    #22469355 - 11/03/15 02:26 AM (8 years, 2 months ago)

That's the point. People seem to think it's liquids first. But liquids pull your hair out if you don't have sterile techniques. Techniques developed from agar work, and a lc inoculated with a clean wedge. Then after it's been inoculated cleanly and colonized, test it on agar to ensure it's clean. Then noc up 200+ jars :excellent:

IMO liquids are the step after agar. Also that LCs go hand in hand with agar.


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InvisibleLocN9ne
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Re: Liquid culture O.K? [Re: Mad Season]
    #22469462 - 11/03/15 04:07 AM (8 years, 2 months ago)

Quote:

Mad Season said:
That's the point. People seem to think it's liquids first. But liquids pull your hair out if you don't have sterile techniques. Techniques developed from agar work, and a lc inoculated with a clean wedge. Then after it's been inoculated cleanly and colonized, test it on agar to ensure it's clean. Then noc up 200+ jars :excellent:

IMO liquids are the step after agar. Also that LCs go hand in hand with agar.



This....if you haven't figured out how to do agar successfully,  don't even bother with LC...they go hand in hand.


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Offlinemigraineur
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Re: Liquid culture O.K? [Re: LocN9ne]
    #22469636 - 11/03/15 05:58 AM (8 years, 2 months ago)

I still don't know why people criticise liquid cultures so much. You can get buckets of liquid culture by using a small squirt from a spore syringe and you don't need to use agar first. Furthermore, you could just as easily make a dirty spore syringe. I had great success when I made and used them but I also had good sterile technique. If you are worried about contamination then don't be lazy with your sterile technique and make test jars.

In addition, doing agar work in a cramped glove box is an unpleasant experience. When I got my flow hood, however, I stopped making liquid cultures and I moved on to grain to grain because the flow hood gave me a lot of open space to work with.


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InvisibleLocN9ne
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Re: Liquid culture O.K? [Re: migraineur] * 1
    #22469691 - 11/03/15 06:30 AM (8 years, 2 months ago)

The agar is the "test jar" except you aren't going to waste spawn, and or lose whatever you spawned to because of molds that you couldn't see... I don't give a fuck how good your sterile technique is, without a clean culture you will fail, or at best have diminished yields....the only exception I can think of is if you are doing an outdoor bed.


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Q&A
US vs. THEM

The more I learn, the less I know.


Edited by LocN9ne (11/03/15 06:31 AM)


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InvisibleSupalemonhaze
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Re: Liquid culture O.K? [Re: LocN9ne]
    #22469890 - 11/03/15 07:49 AM (8 years, 2 months ago)

Yes I agree that one should test the lc with agar or at least a test jar if they dont do agar. As for lcs coming after agar it sure helps but id say its not mandatory. The LC I worked with I made it with a clone taken from the middle of a fruitbody with the utmost care. But if ur inoculating lcs with a syringe ur just asking for trouble. One search on the forums and you can see how much contam issues people have with them. Theres just too many instances where shit can go wrong. Worst part is you won't know if it was you or the syringe. When you do it from agar/clone and it contams at least you know ur the one who fucked up and can be more careful the next time. In my first month doing mycology there was nothing more frustrating than not knowing where my contams were coming from.

As I said I only worked with one LC so far so I might have a different view on things in the future. But *touches wood* so far im just loving LC'S.

As for what migraine said its because of how easily LC's contam. Some are even confident with agar but wont ever do LC's because of this. IME they are right about this because LC is kind of like agar. I have had perfectly good agar plates grow bacteria just from extra heat ( darn cleaner took out my thermometer wire from the terrarium to clean).


Edited by Supalemonhaze (11/03/15 07:56 AM)


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