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OfflinePsilocyBen17
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How to use a Laminar Flow Hood? * 1
    #22464705 - 11/01/15 10:24 PM (8 years, 2 months ago)

Hey guys,

I've never used a flow hood, and don't really need one for where I'm at in this hobby, but I recently found a mycology prof selling his on Kijiji! The price was right so I picked it up. Thing is I've never used one and don't know much about them...

Is it something I could use sitting at a desk in a small room? It sounded like he would put his in the window similar to an air conditioner :shrug:

Anyways I'd like some tips on how to use this thing. The more detailed the better. The only posts I've found on how to build one. So please send some info my way! thx.


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22464729 - 11/01/15 10:27 PM (8 years, 2 months ago)

Have you worked in a Still Air Box yet?


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OfflinePsilocyBen17
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Re: How to use a Laminar Flow Hood? [Re: Willy Wonka]
    #22464745 - 11/01/15 10:30 PM (8 years, 2 months ago)

yeah I've been working in a SAB for 2 years now...

No serious projects on the go right now but this thing was too good of a deal to pass up.


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22464906 - 11/01/15 11:24 PM (8 years, 2 months ago)

Um, pretty easy. Turn it on and let it run for 2 hours before using. Wipe the table top part down with alcohol towel. Always work up stream. Meaning don't have any obstructions between what you're working on and the filter.
The mushroom cultivator has it outlined pretty well on how to use a hood. Buy the book. Read it religiously.


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OfflinePsilocyBen17
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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22464948 - 11/01/15 11:35 PM (8 years, 2 months ago)

OK so I can just do it on my kitchen counter like? Buddy said to put it in a window like I would for an AC....


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Invisibleazur
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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22464954 - 11/01/15 11:38 PM (8 years, 2 months ago)

No. Put it on your counter. I mentioned the two most important things in the previous post


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OfflinePsilocyBen17
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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22464963 - 11/01/15 11:40 PM (8 years, 2 months ago)

Great, thanks for the advice. Appreciate it :thumbup:


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22464967 - 11/01/15 11:43 PM (8 years, 2 months ago)

Chapter 2 in TMC pages 15-40


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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22465044 - 11/02/15 12:11 AM (8 years, 2 months ago)

Jeez there's 25 pages about flowhood work?


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Re: How to use a Laminar Flow Hood? [Re: Inocuole]
    #22465062 - 11/02/15 12:19 AM (8 years, 2 months ago)

Ha. No. It's a chapter on sterile technique and agar culture. Followed by a chapter on grain culture.
Really inoc?  You don't have our bible?  People can say blah blah blah it's 30 years old wha wah, but it is a wealth of information. And frankly, you too goddamn smart not to have the book in your personal library.
Tell your girl to get it for you for Christmas. If she doesn't, i will. 
Don't quote just the last sentence either you perv


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Re: How to use a Laminar Flow Hood? [Re: azur] * 1
    #22465065 - 11/02/15 12:21 AM (8 years, 2 months ago)

Quote:

azur said:

Um, pretty easy. Turn it on and let it run for 2 hours before using.






I don't mean to be a prick, but I don't understand why some people say to leave a hood on for an hour or two before using it.

I've worked in sterile hoods for 20 + years and don't know of a good reason for this type of practice.

I turn a hood on about ten minutes before I use it.


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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202] * 1
    #22465069 - 11/02/15 12:23 AM (8 years, 2 months ago)

I have to correct shit from that book all the damn time, I'm really apprehensive about reading it at this point.


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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202]
    #22465086 - 11/02/15 12:29 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
Quote:

azur said:

Um, pretty easy. Turn it on and let it run for 2 hours before using.






I don't mean to be a prick, but I don't understand why some people say to leave a hood on for an hour or two before using it.

I've worked in sterile hoods for 20 + years and don't know of a good reason for this type of practice.

I turn a hood on about ten minutes before I use it.



Are you working in a clean room?  As in, a room that is positively pressurized?  I'm guessing yes? 
Most people here are in a house kitchen. Very far from clean.
I only run mine for about an hour, but it is in a lab (but not a positively pressurized one).  OP mentions using it in his kitchen, so i recommended 2 hours.
I understand the concept that you should be able to use it within minutes, but if you're not in a "clean room" there's lots of shit moving everywhere. And im high.

Inoc, the info that is correct is so fucking huge that when you're standing on it, the stuff you corrected with look like far away ants


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22465098 - 11/02/15 12:34 AM (8 years, 2 months ago)

Also, op, do not use your hood for cleaning up contamed plates. Use sab for that


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OfflineRJ Tubs 202
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Re: How to use a Laminar Flow Hood? [Re: azur] * 1
    #22465103 - 11/02/15 12:36 AM (8 years, 2 months ago)

Quote:

azur said:
Are you working in a clean room?  As in, a room that is positively pressurized?  I'm guessing yes? 





I've never worked in a positively pressurized room.

A hood is an air filter.  The air that comes thru it is clean.

I just don't understand how an hour or two running before doing transfers would help anything.


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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202]
    #22465235 - 11/02/15 02:08 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
I've never worked in a positively pressurized room.

A hood is an air filter.  The air that comes thru it is clean.

I just don't understand how an hour or two running before doing transfers would help anything.




Quote:

I understand the concept that you should be able to use it within minutes, but if you're not in a "clean room" there's lots of shit moving everywhere. And im high.




^^^because this


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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202]
    #22465489 - 11/02/15 06:42 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
Quote:

azur said:
Are you working in a clean room?  As in, a room that is positively pressurized?  I'm guessing yes? 





I've never worked in a positively pressurized room.

A hood is an air filter.  The air that comes thru it is clean.

I just don't understand how an hour or two running before doing transfers would help anything.




During those 2 hours it will clean most if not all of the air. I don't have a flow hood so im only imagining it here. When the flow hood blows it disturbes the air around the flow. The effect of this creates vortexes that will turn inside the flow which the flow in turn blows it in your work. I think this has more chance of happening the slower the flow is moving. Sort of like when you are standing near a fast passing bus. When it passes you can feel a gust blowing toward the middle of the path it took.

I think you would be able to see this with incense or some other type of smoke. Its more going the extra mile than a requirement, which can never hurt.

As I said I don't have a hood so there might be a chance im wrong so it would be nice to see someone with actual experience with a hood give his 2c about this. It makes perfect sense in my mind though.


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Re: How to use a Laminar Flow Hood? [Re: blindingleaf]
    #22465587 - 11/02/15 07:29 AM (8 years, 2 months ago)

I wish I hate a nice place with one of these to use..


Edited by Aliasbane (11/02/15 07:32 AM)


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Re: How to use a Laminar Flow Hood? [Re: Aliasbane]
    #22465642 - 11/02/15 07:44 AM (8 years, 2 months ago)

Quote:

Aliasbane said:
I wish I hate a nice place with one of these to use..




No shit. I just went to a lab supply warehouse to buy some plastic petris and asked if he ever had any laminars. He said ye for 2k euro I can import a killer one for you from italy. I was like  :freshwtf:


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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22465794 - 11/02/15 08:36 AM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
Quote:

Aliasbane said:
I wish I hate a nice place with one of these to use..




No shit. I just went to a lab supply warehouse to buy some plastic petris and asked if he ever had any laminars. He said ye for 2k euro I can import a killer one for you from italy. I was like  :freshwtf:




Defintely props to the thread starter for finding a great deal on one!

or I should bug ya know all my friends in med school and are chemists/biologist and just borrow a lab for a few hours lol


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OfflinePsilocyBen17
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Re: How to use a Laminar Flow Hood? [Re: Aliasbane]
    #22465887 - 11/02/15 09:18 AM (8 years, 2 months ago)

The one I found was $350 but I recon it had about $200 worth of materials in it. If you are technically inclined you could build one yourself. Keep your eyes peeled on Kijiji in the meantime. . Hard to find one but it does happen. ! When I used to live in Toronto mycologist friends of mine used to scout out laboratories and microchip companies that were closing and go to the auctions...thats another way to find a deal!


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22465973 - 11/02/15 09:49 AM (8 years, 2 months ago)

Darn how I wish we didn't bully all those geeks when I was in school.

Serves me right I spose.


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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22466167 - 11/02/15 10:51 AM (8 years, 2 months ago)

love the sig brutha


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22468303 - 11/02/15 08:05 PM (8 years, 2 months ago)

Thanks.

Here noone really knows about mycology. Thankfully yesterday i visted that lab supply place I talked about and they have a lotta shit I will need. Agar, tubes for slants, racks, desiccant gel magnetic stirers(expensive though) and all other cool stuff. Hell I might even buy a hepa in due time. Gotta wait at least a year though till I get back on my feet moneywise


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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22468806 - 11/02/15 10:00 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:

During those 2 hours it will clean most if not all of the air.





What air?          The air in the room?

If so, why would anyone try to clean the air in a room?    A transfer or inoculation is done in front of the filter.


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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22469010 - 11/02/15 11:12 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
Thanks.

Here noone really knows about mycology. Thankfully yesterday i visted that lab supply place I talked about and they have a lotta shit I will need. Agar, tubes for slants, racks, desiccant gel magnetic stirers(expensive though) and all other cool stuff. Hell I might even buy a hepa in due time. Gotta wait at least a year though till I get back on my feet moneywise




Don't loose patience in the Shroomery man. People here know a lot. You just gotta sift through a lot of shit to find the good stuff.

Also, don't get jipped on supplies. A lot of mycology suppliers sell the same shit you can get at Walmart for 10x the price.


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22469551 - 11/03/15 05:01 AM (8 years, 2 months ago)

Telling people here they know nothing about mycology seems like a great way to get help.


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Re: How to use a Laminar Flow Hood? [Re: Inocuole]
    #22469596 - 11/03/15 05:29 AM (8 years, 2 months ago)



This is how Ive been setting my flowhood up lately, its in a room about the size of a childs bedroom, maybe a bit smaller.

Before I use my hood I hit the face with lysol, let it sit a couple min the turn it own, wipe down the tabletop (I set a big sheet of glass down, its just so easy to clean), turn the hood on.
I let it run a couple hours usually, sometimes more or less depending on the kind of work Im doing and how much prep work I need beforehand. Usually by the time Im set up and out of the shower its been about an hour. If Im just doing some agar work Ill run it less, 20 min to half hour, never had a problem.
If  its in a room with heat/cool ducts either block them off or turn it off. I put a towel under the door.

The sterile technique needs to be a bit better in front of a hood IME. I wear clothes fresh out of laundry, clean tyvek sleeves, face mask, gloves. Everythinh going in front of the hood gets wiped down with ISO pretty well first.
The thing to remember with a hood is the air is moving so never move anything between your filter and your work... it can blow nasties right into it.

Pretty much the same sterile tek as a still air box. I love my FH <3


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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202]
    #22469625 - 11/03/15 05:50 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
Quote:

Supalemonhaze said:

During those 2 hours it will clean most if not all of the air.





What air?          The air in the room?

If so, why would anyone try to clean the air in a room?    A transfer or inoculation is done in front of the filter.




Most of us that cultivate at home have to battle pretty fierce contams. Our houses are full of the normal dust and mold and can't do much about it. Of course you work in the sterile airflow man.
But if scrubbing the air in the room gives any edge, and its as easy as flipping a switch, why wouldn't you? Its not gunna take much life off the filter, especially with a decent pre filter.
A lot of us hit the face of the hood with lysol real quick before turning it on too.


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Re: How to use a Laminar Flow Hood? [Re: mushpunx]
    #22469629 - 11/03/15 05:53 AM (8 years, 2 months ago)

My filter is 36 years old and works just great.


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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22469637 - 11/03/15 05:58 AM (8 years, 2 months ago)

Quote:

azur said:
My filter is 36 years old and works just great.





Wow! Right though? Not hurting the hood. Mine is on overnight frequently if I poured plates or whatever

I usually know when I have work to do so I just remember to hit the switch when I walk by.


Edited by mushpunx (11/03/15 06:01 AM)


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Re: How to use a Laminar Flow Hood? [Re: mushpunx]
    #22469645 - 11/03/15 06:05 AM (8 years, 2 months ago)

Oct. 1979!


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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22469804 - 11/03/15 07:16 AM (8 years, 2 months ago)

Is that when you bought/built it yourself or was there a previous owner? It was for cultivation?

Awesome


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Re: How to use a Laminar Flow Hood? [Re: Inocuole]
    #22469842 - 11/03/15 07:29 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
Quote:

Supalemonhaze said:

During those 2 hours it will clean most if not all of the air.





What air?          The air in the room?

If so, why would anyone try to clean the air in a room?    A transfer or inoculation is done in front of the filter.




I get a feeling you barely read my post. The clean air from the hood will agitate the dirty air from the room and this MIGHT find its way into your media. Sure its an extra precaution but imo if your cautious enough to build a hood its silly not to switch it on beforehand.

Quote:

Inocuole said:
Telling people here they know nothing about mycology seems like a great way to get help.




I meant in my country lmao.


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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22469854 - 11/03/15 07:34 AM (8 years, 2 months ago)

Hah I took that the wrong way too. Thought you meant "here" as in "here at thr shroomery".

There is a wealth of knowledge regarding mycology and certainly home cultuvation here!
What country are you from? That's awesome you have a supply store man I hate running out of supplies between orders


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Re: How to use a Laminar Flow Hood? [Re: mushpunx]
    #22469861 - 11/03/15 07:36 AM (8 years, 2 months ago)

Well, i wasn't born until dec of 79, so, yea. I bought it used. Tore it apart and cleaned it up. Painted it. Was going to change the filter as it had the oct 79 date on it, but decided to try it and it's great


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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22469920 - 11/03/15 07:59 AM (8 years, 2 months ago)

azur's hood is legit, looks so fucking awesome!!


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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22469922 - 11/03/15 08:01 AM (8 years, 2 months ago)

Quote:

azur said:
Well, i wasn't born until dec of 79, so, yea. I bought it used. Tore it apart and cleaned it up. Painted it. Was going to change the filter as it had the oct 79 date on it, but decided to try it and it's great





Yea I didn't think you were muvh older than myself :lol:

Was hoping to get some first hand insights into home cultivation of magic mushrooms in the 80s prior to PF tek

Ive seen his hood man it is freaking beautifull


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Re: How to use a Laminar Flow Hood? [Re: mushpunx]
    #22470176 - 11/03/15 09:31 AM (8 years, 2 months ago)

Quote:

mushpunx said:


This is how Ive been setting my flowhood up lately, its in a room about the size of a childs bedroom, maybe a bit smaller.

  <3




My new hood is about the SIZE of a childs bedroom. Fuck its a big SOB. Hope I can find a spot for it soon.


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Re: How to use a Laminar Flow Hood? [Re: mushpunx]
    #22470218 - 11/03/15 09:43 AM (8 years, 2 months ago)

Quote:

mushpunx said:
Hah I took that the wrong way too. Thought you meant "here" as in "here at thr shroomery".

There is a wealth of knowledge regarding mycology and certainly home cultuvation here!
What country are you from? That's awesome you have a supply store man I hate running out of supplies between orders




Small country in the Mediterranean. To give you an idea of how small it is we only have one public hospital and one university which doesn't even have a mycology section. They are planning to build a private American university but I dunno when or if it will happen. It would be nice to see someone teaching mycology here even though I have no chance of further education both public or private. At least it will increase interest on the subject and would increase specialised equipment. Nothing fancy you know. Maybe sfd's and injection ports. I haven't been able to find those at the lab supply place.

Nah man im eternally grateful for shroomery. If it wasn't for this site I would have probably failed much more times than I actually did. Imagine having the "scientists" from bluelight or reddit helping you. :horrified: ur better off alone lmao.


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22470225 - 11/03/15 09:45 AM (8 years, 2 months ago)

Quote:

PsilocyBen17 said:
Quote:

mushpunx said:


This is how Ive been setting my flowhood up lately, its in a room about the size of a childs bedroom, maybe a bit smaller.

  <3




My new hood is about the SIZE of a childs bedroom. Fuck its a big SOB. Hope I can find a spot for it soon.




:freshwtf: good luck with that lol


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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22470285 - 11/03/15 09:59 AM (8 years, 2 months ago)

There are lots of "scientists" on here man, they just don't wear the labcoats. I live in Canada and a degree in Mycology is just as inaccessible here.


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22470303 - 11/03/15 10:04 AM (8 years, 2 months ago)

Quote:

PsilocyBen17 said:
There are lots of "scientists" on here man, they just don't wear the labcoats. I live in Canada and a degree in Mycology is just as inaccessible here.




What I meant is by the way they talk on reddit one would assume they are freakin Einsten. But ull just lol when you see the misinformation flowing and the poor newbies actually thanking them for it. Its just horrible.


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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22470339 - 11/03/15 10:16 AM (8 years, 2 months ago)

Well fortunately you don't have to be a scientist to grow mushrooms :lol:

Really though from our clandestine spaces what we do is pretty much what a professional spawn lab would do only on a much smaller scale.

And honestly the vast majority of us being self educated Im pretty sure that those professionals would be quite impressed with some of the grows on this site!



So this enormous flowhood! Details?


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Re: How to use a Laminar Flow Hood? [Re: mushpunx]
    #22471376 - 11/03/15 02:38 PM (8 years, 2 months ago)

With a flow hood, how far away should the intake be from the wall?


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22471380 - 11/03/15 02:38 PM (8 years, 2 months ago)

My intake is on top and is about a foot from the ceiling


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Re: How to use a Laminar Flow Hood? [Re: mushpunx]
    #22471442 - 11/03/15 02:48 PM (8 years, 2 months ago)

Quote:

mushpunx said:




So this enormous flowhood! Details?




Fuck man. the thing is 5' x 5'. Much bigger than what I need and it weighs a TON! quite impractical as I can't even lift it myself. Am clearing out a spot in the house for it where it can hopefully live permanently. I bought it from some whacko on Kijiji who used it for mycology and had a friend pick it up. I wouldnt have bought it if I had have realized the size, but atleast the price was right. I figure atleast I'll be able to get what I paid for it back if I ever decide to sell it down the line :shrug:


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Edited by PsilocyBen17 (11/03/15 03:24 PM)


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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22471463 - 11/03/15 02:51 PM (8 years, 2 months ago)

Quote:

azur said:
My intake is on top and is about a foot from the ceiling







Mine will be set up exactly like the one in Mushpunx sig. I guess thats far enough from the wall? Fuck this thing might suck me into it if I turn it on.


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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22471512 - 11/03/15 03:03 PM (8 years, 2 months ago)

5x5 is sweet. It'll be awesome to spawn to bulk in front of.


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Re: How to use a Laminar Flow Hood? [Re: azur] * 1
    #22471602 - 11/03/15 03:21 PM (8 years, 2 months ago)

Quote:

azur said:
5x5 is sweet. It'll be awesome to spawn to bulk in front of.




This implies a certain amount of danger with spawning to bulk in general... what would you estimate you're contam rate to be if you spawned to bulk without a clean room i.e. in the average household kitchen/living room/bedroom??


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Re: How to use a Laminar Flow Hood? [Re: invitro]
    #22471660 - 11/03/15 03:34 PM (8 years, 2 months ago)

Oh,  i wasn't implying anything. Just better to wipe your ass one more time after the paper is clean.
I don't grow in my house anymore. But a house is absolutely filthy compared to a clean room.


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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22472076 - 11/03/15 05:04 PM (8 years, 2 months ago)

Quote:

azur said:
Oh,  i wasn't implying anything. Just better to wipe your ass one more time after the paper is clean.
I don't grow in my house anymore. But a house is absolutely filthy compared to a clean room.





Hahaha nice euphemism

Actually I know some folk who spawn to bulk under flow hood, why not right?


5 X 5? That's a bit intimidating.  Itll be a joy to work in front of! You could be flailing your arms all over the place and still be in the flow. Spawn bags will be a pleasure.
Could be doing bag to bag transfers even like you're pouring a bowl of ceral with a hood that tall :lol:


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Re: How to use a Laminar Flow Hood? [Re: mushpunx]
    #22472366 - 11/03/15 05:54 PM (8 years, 2 months ago)

You could fuck a hooker bareback in front of it


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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22473781 - 11/03/15 10:26 PM (8 years, 2 months ago)

Quote:

Supalemonhaze said:

I get a feeling you barely read my post. The clean air from the hood will agitate the dirty air from the room and this MIGHT find its way into your media. Sure its an extra precaution but imo if your cautious enough to build a hood its silly not to switch it on beforehand.






You're right, it won't hurt . . .  1 or 2 hours simply seems excessive, since even a small hood moves 300 - 500 cubic feet of air per minute.

Realize that contamination generally comes from poor sterile technique, and not from outside dirty air entering the hood. With good sterile methods, we should be able to do clean work with our dog sitting on our lap.


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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22475169 - 11/04/15 09:38 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
Quote:

Supalemonhaze said:

I get a feeling you barely read my post. The clean air from the hood will agitate the dirty air from the room and this MIGHT find its way into your media. Sure its an extra precaution but imo if your cautious enough to build a hood its silly not to switch it on beforehand.






You're right, it won't hurt . . .  1 or 2 hours simply seems excessive, since even a small hood moves 300 - 500 cubic feet of air per minute.

Realize that contamination generally comes from poor sterile technique, and not from outside dirty air entering the hood. With good sterile methods, we should be able to do clean work with our dog sitting on our lap.




Exactly thats why a SAB is generally enough to be able to work. Its just preference to switch it on beforehand. Just like cleaning an entire room with bleach water and spraying oust before using a SAB/ spraying the filter before using. Its not necessary but it can't hurt.


Quote:

azur said:
You could fuck a hooker bareback in front of it





Lmao.. tell me the benefits of this again? Avoiding heat strokes maybe? :p


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