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OfflinePsilocyBen17
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Registered: 10/20/13
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How to use a Laminar Flow Hood? * 1
    #22464705 - 11/01/15 10:24 PM (8 years, 2 months ago)

Hey guys,

I've never used a flow hood, and don't really need one for where I'm at in this hobby, but I recently found a mycology prof selling his on Kijiji! The price was right so I picked it up. Thing is I've never used one and don't know much about them...

Is it something I could use sitting at a desk in a small room? It sounded like he would put his in the window similar to an air conditioner :shrug:

Anyways I'd like some tips on how to use this thing. The more detailed the better. The only posts I've found on how to build one. So please send some info my way! thx.


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InvisibleWilly Wonka
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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22464729 - 11/01/15 10:27 PM (8 years, 2 months ago)

Have you worked in a Still Air Box yet?


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OfflinePsilocyBen17
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Re: How to use a Laminar Flow Hood? [Re: Willy Wonka]
    #22464745 - 11/01/15 10:30 PM (8 years, 2 months ago)

yeah I've been working in a SAB for 2 years now...

No serious projects on the go right now but this thing was too good of a deal to pass up.


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Invisibleazur
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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22464906 - 11/01/15 11:24 PM (8 years, 2 months ago)

Um, pretty easy. Turn it on and let it run for 2 hours before using. Wipe the table top part down with alcohol towel. Always work up stream. Meaning don't have any obstructions between what you're working on and the filter.
The mushroom cultivator has it outlined pretty well on how to use a hood. Buy the book. Read it religiously.


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OfflinePsilocyBen17
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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22464948 - 11/01/15 11:35 PM (8 years, 2 months ago)

OK so I can just do it on my kitchen counter like? Buddy said to put it in a window like I would for an AC....


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Invisibleazur
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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22464954 - 11/01/15 11:38 PM (8 years, 2 months ago)

No. Put it on your counter. I mentioned the two most important things in the previous post


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A cube is NOT a cube.

FALL IN LOVE WITH LC
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OfflinePsilocyBen17
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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22464963 - 11/01/15 11:40 PM (8 years, 2 months ago)

Great, thanks for the advice. Appreciate it :thumbup:


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Invisibleazur
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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22464967 - 11/01/15 11:43 PM (8 years, 2 months ago)

Chapter 2 in TMC pages 15-40


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InvisibleInocuole
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Re: How to use a Laminar Flow Hood? [Re: azur]
    #22465044 - 11/02/15 12:11 AM (8 years, 2 months ago)

Jeez there's 25 pages about flowhood work?


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Invisibleazur
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Re: How to use a Laminar Flow Hood? [Re: Inocuole]
    #22465062 - 11/02/15 12:19 AM (8 years, 2 months ago)

Ha. No. It's a chapter on sterile technique and agar culture. Followed by a chapter on grain culture.
Really inoc?  You don't have our bible?  People can say blah blah blah it's 30 years old wha wah, but it is a wealth of information. And frankly, you too goddamn smart not to have the book in your personal library.
Tell your girl to get it for you for Christmas. If she doesn't, i will. 
Don't quote just the last sentence either you perv


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FALL IN LOVE WITH LC
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OfflineRJ Tubs 202
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Re: How to use a Laminar Flow Hood? [Re: azur] * 1
    #22465065 - 11/02/15 12:21 AM (8 years, 2 months ago)

Quote:

azur said:

Um, pretty easy. Turn it on and let it run for 2 hours before using.






I don't mean to be a prick, but I don't understand why some people say to leave a hood on for an hour or two before using it.

I've worked in sterile hoods for 20 + years and don't know of a good reason for this type of practice.

I turn a hood on about ten minutes before I use it.


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InvisibleInocuole
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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202] * 1
    #22465069 - 11/02/15 12:23 AM (8 years, 2 months ago)

I have to correct shit from that book all the damn time, I'm really apprehensive about reading it at this point.


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Invisibleazur
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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202]
    #22465086 - 11/02/15 12:29 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
Quote:

azur said:

Um, pretty easy. Turn it on and let it run for 2 hours before using.






I don't mean to be a prick, but I don't understand why some people say to leave a hood on for an hour or two before using it.

I've worked in sterile hoods for 20 + years and don't know of a good reason for this type of practice.

I turn a hood on about ten minutes before I use it.



Are you working in a clean room?  As in, a room that is positively pressurized?  I'm guessing yes? 
Most people here are in a house kitchen. Very far from clean.
I only run mine for about an hour, but it is in a lab (but not a positively pressurized one).  OP mentions using it in his kitchen, so i recommended 2 hours.
I understand the concept that you should be able to use it within minutes, but if you're not in a "clean room" there's lots of shit moving everywhere. And im high.

Inoc, the info that is correct is so fucking huge that when you're standing on it, the stuff you corrected with look like far away ants


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Invisibleazur
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Re: How to use a Laminar Flow Hood? [Re: PsilocyBen17]
    #22465098 - 11/02/15 12:34 AM (8 years, 2 months ago)

Also, op, do not use your hood for cleaning up contamed plates. Use sab for that


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
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OfflineRJ Tubs 202
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Re: How to use a Laminar Flow Hood? [Re: azur] * 1
    #22465103 - 11/02/15 12:36 AM (8 years, 2 months ago)

Quote:

azur said:
Are you working in a clean room?  As in, a room that is positively pressurized?  I'm guessing yes? 





I've never worked in a positively pressurized room.

A hood is an air filter.  The air that comes thru it is clean.

I just don't understand how an hour or two running before doing transfers would help anything.


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Invisibleblindingleaf
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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202]
    #22465235 - 11/02/15 02:08 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
I've never worked in a positively pressurized room.

A hood is an air filter.  The air that comes thru it is clean.

I just don't understand how an hour or two running before doing transfers would help anything.




Quote:

I understand the concept that you should be able to use it within minutes, but if you're not in a "clean room" there's lots of shit moving everywhere. And im high.




^^^because this


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InvisibleSupalemonhaze
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Re: How to use a Laminar Flow Hood? [Re: RJ Tubs 202]
    #22465489 - 11/02/15 06:42 AM (8 years, 2 months ago)

Quote:

RJ Tubs 202 said:
Quote:

azur said:
Are you working in a clean room?  As in, a room that is positively pressurized?  I'm guessing yes? 





I've never worked in a positively pressurized room.

A hood is an air filter.  The air that comes thru it is clean.

I just don't understand how an hour or two running before doing transfers would help anything.




During those 2 hours it will clean most if not all of the air. I don't have a flow hood so im only imagining it here. When the flow hood blows it disturbes the air around the flow. The effect of this creates vortexes that will turn inside the flow which the flow in turn blows it in your work. I think this has more chance of happening the slower the flow is moving. Sort of like when you are standing near a fast passing bus. When it passes you can feel a gust blowing toward the middle of the path it took.

I think you would be able to see this with incense or some other type of smoke. Its more going the extra mile than a requirement, which can never hurt.

As I said I don't have a hood so there might be a chance im wrong so it would be nice to see someone with actual experience with a hood give his 2c about this. It makes perfect sense in my mind though.


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OfflineAliasbane
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Re: How to use a Laminar Flow Hood? [Re: blindingleaf]
    #22465587 - 11/02/15 07:29 AM (8 years, 2 months ago)

I wish I hate a nice place with one of these to use..


Edited by Aliasbane (11/02/15 07:32 AM)


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InvisibleSupalemonhaze
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Re: How to use a Laminar Flow Hood? [Re: Aliasbane]
    #22465642 - 11/02/15 07:44 AM (8 years, 2 months ago)

Quote:

Aliasbane said:
I wish I hate a nice place with one of these to use..




No shit. I just went to a lab supply warehouse to buy some plastic petris and asked if he ever had any laminars. He said ye for 2k euro I can import a killer one for you from italy. I was like  :freshwtf:


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OfflineAliasbane
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Re: How to use a Laminar Flow Hood? [Re: Supalemonhaze]
    #22465794 - 11/02/15 08:36 AM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
Quote:

Aliasbane said:
I wish I hate a nice place with one of these to use..




No shit. I just went to a lab supply warehouse to buy some plastic petris and asked if he ever had any laminars. He said ye for 2k euro I can import a killer one for you from italy. I was like  :freshwtf:




Defintely props to the thread starter for finding a great deal on one!

or I should bug ya know all my friends in med school and are chemists/biologist and just borrow a lab for a few hours lol


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