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FritztamineTheCat
Former Frequent Lurker

Registered: 10/24/15
Posts: 82
Last seen: 7 years, 6 months
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Experimental Acacia confusa Substrate P cubensis Grow
#22464144 - 11/01/15 08:14 PM (8 years, 2 months ago) |
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**PLEASE DONT BE SCARED AWAY FROM THE BODY OF TEXT I SWEAR IM NOT WASTING BREATH**
Hello all i decided to start off my shroomery career with something beneficial that should help the fellow experimenter looking to improve quality of their carpophores and other fungal bodies since you were all so helpful in providing quality information that lead me to become very knowledgeable in the area of cultivation of many active species containing psilocybin/psilocin.
Anyways the whole purpose of this experiment is to augment cubensis into creating a more potent fruit containing larger amounts of psilocybin/psilocin. I personally have noticed that adding DMT/Mimosa bark to the substrate resulted in lil to no difference and for a long time i chalked it up as bunked and thought that making mushrooms more potent were unlikely to be practically applied with repeatable results.
That is until i came across this document on here so i decided to do a rushed experiment with acacia to see if its NMT content made a difference with resulting in a more potent fruit. And from personal bio assays of what would be normally a micro dose of this particular clone culture of cubensis of just .3g (just below threshold) which would normally not give me noticeable effects, I was surprised to find that .3g of material from the small experiment grown with a random amount of acacia bark was quite effective at bringing me to a strong ++. So i am repeating it with a control group and and additional different strain side by side.
The small experiment that was performed before was very half assed with no control group, mostly because i thought it to be trivial as I have done experiments before with mimosa hostilis that resulted in an unnoticeable difference between other standardly grown cubensis mushrooms. So i wont go in to detail with the older results more than I have already stated but i will say they were promising enough for me to create an account and document it on here to share with you guys!
So the goal experiment is to set up 4x 105qt monotubs with 2 different strains, 2 tubs of each strain one being a control tub and the other being the experimental substrate tub. In this case it will be an albino A+ clone culture of an already potent agar isolate and a PES Amazonian multispore culture both acquired from one if the shroomerys sponsor.
All 4 tubs will have FAE holes with loose poly with a 4" deep substrate containing a pasteurized mix of:
8 parts shredded straw 4 parts horse manure 3 parts vermiculite 2 parts coir 1/4 cup gypsum per tub
(each tub will be inoculated with 5 qts of wild bird seed spawn)
The experimental tubs will have acacia confusa bark added to it that has been personally extracted and known to contain atleast 0.75% nmt and about 0.6% dmt these numbers aren't precise but it is just how my extraction turned out.
I have the wild bird seed jars already inoculated (3 days ago) so i will update with some pictures tomorrow or the next day when i see some growth starting but before i started i wanted to see what you guys that about how much acacia bark to put in each tub which have about 23 qts of substrate total and usually yield about 6-10oz per tub depending on strain so the amount would need to be significant enough to increase the potency by id say at least 35% to be noticeable in a blind trial so i was hoping to get some advice on how much to use to make this happen my goal is to give it the necessary amount of material to achieve a 50% increase in potency.
Thanks guys for all the things you helped me achieve and i hope this doesn't bore you because it doesnt have photos yet but i will not let this thread die and i promise to keep you updated all the way to the end i always hate cliff hanger experiment threads just like you, So i hope you like and follow my first post
-much love thecat
-------------------- My Pokemon Collection Ps. cubenis-Albino A+, Golden Teacher, Mazatapec, Puerto Rico, PE, PE Uncut, APE, Falbino, PFRS, Orissa India, Ps. azurescens Pan. tampanensis Ps. tropicalis Ps. galindoi Ps. stutzii Ps. cyanescens Ps. ovoideocystidiata Ps. hoogshagenii var convexa (semperviva)
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the_r3dz
Trich Propagator



Registered: 03/26/15
Posts: 5,709
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Re: Experimental Acacia confusa Substrate P cubensis Grow [Re: FritztamineTheCat]
#22464529 - 11/01/15 09:42 PM (8 years, 2 months ago) |
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Don't have much to add but would love to be updated on this experiment!
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FritztamineTheCat
Former Frequent Lurker

Registered: 10/24/15
Posts: 82
Last seen: 7 years, 6 months
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Re: Experimental Acacia confusa Substrate P cubensis Grow [Re: FritztamineTheCat]
#22464603 - 11/01/15 09:58 PM (8 years, 2 months ago) |
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I also plan doing more than just a bio assay on the resulting materials. My plan was to grind all the fruit bodies from each tub separately into a powder, mixing it well, and then take 2 separate 1 gram samples from each tubs mushroom powder to test for varience in the material and doing a methanol/ethanol extractions on each sample separately in the same conditions at the same time. Once i have the 8 separate labeled samples I will dissolve each one in a vial with 4ml and testing a drop from each sample with a reagent that test for indole alkaloids the first test should test positive for indoles in all the samples. Then start diluting the samples by 25% volume increments testing for indoles each time until the samples stop testing positive then compare the volumes of solution each sample required to obtain a negative test result for indole alkaloids to get a more statistical analysis of the difference in potency it makes. What dyou guys think?
-------------------- My Pokemon Collection Ps. cubenis-Albino A+, Golden Teacher, Mazatapec, Puerto Rico, PE, PE Uncut, APE, Falbino, PFRS, Orissa India, Ps. azurescens Pan. tampanensis Ps. tropicalis Ps. galindoi Ps. stutzii Ps. cyanescens Ps. ovoideocystidiata Ps. hoogshagenii var convexa (semperviva)
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Experimental Acacia confusa Substrate P cubensis Grow [Re: FritztamineTheCat]
#22469239 - 11/03/15 01:07 AM (8 years, 2 months ago) |
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I member from a journal reference I cited here, N,N-Dimetyltryptamine was shown not to measurably increase potency. I'm not sure about N-methyltryptamine (is that substance active?)
Anyway, a lot of discussion you can pull up searching in this forum for potency + ( DMT | DPT | Tryptamine | Tryptophan )*
DPT, I believe, was shown to be 4-hydroxylated.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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FritztamineTheCat
Former Frequent Lurker

Registered: 10/24/15
Posts: 82
Last seen: 7 years, 6 months
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Re: Experimental Acacia confusa Substrate P cubensis Grow [Re: micro]
#22488409 - 11/06/15 09:23 PM (8 years, 2 months ago) |
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True based on the graph i posted an image of it takes 32 parts DMT to create 1 part 4-HO-DMT, which would be highly inefficient. But also according to the same graph it only takes 2 parts NMT to create 1 part 4-HO-DMT. This information gives evidence that supports the theory that DMT is not a major step in the metabolitic pathway that the mushrooms use to produce psilocin/psilocybin and that NMT is more readily absorbed and used by the fungus in the creation of psilocin. So based on this information, and with a goal of obtaining a harvest of fruits at-least 150% the potency of the control group, i came up with the following
And because acacia is said to have .5% to up to 1.2% NMT i am going to assume it to be .8% NMT since i dont know for sure even with my extractions in it they are far from perfect. And since cubensis has about .6% psilocin/cybin and this tub/culture yields about 8-10oz first flush its safe to say roughly 1.35-1.75grams of psilocybin/cin is produced so we need to add at least 1.75g nmt or ~220g acacia bark which isn't very costly at all so I'm going to round it up to 250g debating on 300g not sure these numbers could all be different from the realistic numbers.
-------------------- My Pokemon Collection Ps. cubenis-Albino A+, Golden Teacher, Mazatapec, Puerto Rico, PE, PE Uncut, APE, Falbino, PFRS, Orissa India, Ps. azurescens Pan. tampanensis Ps. tropicalis Ps. galindoi Ps. stutzii Ps. cyanescens Ps. ovoideocystidiata Ps. hoogshagenii var convexa (semperviva)
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FritztamineTheCat
Former Frequent Lurker

Registered: 10/24/15
Posts: 82
Last seen: 7 years, 6 months
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Re: Experimental Acacia confusa Substrate P cubensis Grow [Re: FritztamineTheCat]
#22488454 - 11/06/15 09:33 PM (8 years, 2 months ago) |
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Also when i post an image on here does it contain meta deta on it or should that be taken off before posting it? Never could find an answer regarding that.
-------------------- My Pokemon Collection Ps. cubenis-Albino A+, Golden Teacher, Mazatapec, Puerto Rico, PE, PE Uncut, APE, Falbino, PFRS, Orissa India, Ps. azurescens Pan. tampanensis Ps. tropicalis Ps. galindoi Ps. stutzii Ps. cyanescens Ps. ovoideocystidiata Ps. hoogshagenii var convexa (semperviva)
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Experimental Acacia confusa Substrate P cubensis Grow [Re: FritztamineTheCat]
#22488553 - 11/06/15 10:01 PM (8 years, 2 months ago) |
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Hmm... Yeah, that paper looks familiar.
I wonder if it's the same one I read :o
IDK about metadata; it *can* be the source of some XSS stuff.
You'd have to upload and re-download it and see if it strips it off.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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FritztamineTheCat
Former Frequent Lurker

Registered: 10/24/15
Posts: 82
Last seen: 7 years, 6 months
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quick update [Re: micro]
#22648504 - 12/13/15 12:30 AM (8 years, 1 month ago) |
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Ok sorry for my absence i actually had a nasty trich infestation so i stopped using coir and changed my tek to just straight straw to eliminate hassles of infection. Also scaled it down to just 100g of acacia for the experiment because i already lost a bunch to infection. Here are some pics from yesterday after spawning 7 qts of popcorn spawn per tub one is just straw the other straw plus acacia bark both tubs an agar isolate of a giant albino clone that i have got 5" capsthat are so big its insane.
-------------------- My Pokemon Collection Ps. cubenis-Albino A+, Golden Teacher, Mazatapec, Puerto Rico, PE, PE Uncut, APE, Falbino, PFRS, Orissa India, Ps. azurescens Pan. tampanensis Ps. tropicalis Ps. galindoi Ps. stutzii Ps. cyanescens Ps. ovoideocystidiata Ps. hoogshagenii var convexa (semperviva)
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FritztamineTheCat
Former Frequent Lurker

Registered: 10/24/15
Posts: 82
Last seen: 7 years, 6 months
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-------------------- My Pokemon Collection Ps. cubenis-Albino A+, Golden Teacher, Mazatapec, Puerto Rico, PE, PE Uncut, APE, Falbino, PFRS, Orissa India, Ps. azurescens Pan. tampanensis Ps. tropicalis Ps. galindoi Ps. stutzii Ps. cyanescens Ps. ovoideocystidiata Ps. hoogshagenii var convexa (semperviva)
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pineninja
Dream Weaver



Registered: 08/17/14
Posts: 12,468
Loc: South
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-------------------- Just a fool on the hill.
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FritztamineTheCat
Former Frequent Lurker

Registered: 10/24/15
Posts: 82
Last seen: 7 years, 6 months
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Also the tubs are 105qt with 4" substrate depth which is a bit big but it's all i had and I've used them before with lil issue. the straw was soaked in dilute bleach and dawn water over night then thoroughly rinsed and pasteurized for 90mins. And i haven't melted the whole for fae yet was going to let the spawn finish colonizing the straw. I'll put up some better photos next time since these aren't the greatest quality as i was in a rush.
-------------------- My Pokemon Collection Ps. cubenis-Albino A+, Golden Teacher, Mazatapec, Puerto Rico, PE, PE Uncut, APE, Falbino, PFRS, Orissa India, Ps. azurescens Pan. tampanensis Ps. tropicalis Ps. galindoi Ps. stutzii Ps. cyanescens Ps. ovoideocystidiata Ps. hoogshagenii var convexa (semperviva)
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FritztamineTheCat
Former Frequent Lurker

Registered: 10/24/15
Posts: 82
Last seen: 7 years, 6 months
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And just wondering if anyone else out there had ran straight straw in a monotub and if they could chime in on some yields they got from a single tub and also can tell me what size tub they used. Id like to hear from 105qt users but i know there arent many so I'd like to hear anyone's yield story and would like some pics even more. also i heard its best not to case straw is this true?
-------------------- My Pokemon Collection Ps. cubenis-Albino A+, Golden Teacher, Mazatapec, Puerto Rico, PE, PE Uncut, APE, Falbino, PFRS, Orissa India, Ps. azurescens Pan. tampanensis Ps. tropicalis Ps. galindoi Ps. stutzii Ps. cyanescens Ps. ovoideocystidiata Ps. hoogshagenii var convexa (semperviva)
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