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LumpyNutz
Bad fisH <*))))<



Registered: 07/14/14
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WBS topper? (lid)
#22463831 - 11/01/15 07:13 PM (8 years, 2 months ago) |
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Hi all, been lingering a while, checking out teks for WBS jars. Ended up following Doc34's tek. I noc'd em with a Penis Envy spore syringe and followed the tek to the T
But my question is: Do I keepp the 2 coffee filters off the top after sterilization/innoc?? I got the lid under them with the 1/4" hole stuffed with polyfill so im wondering is the coffee filters necessary after innoc?? will they reduce FAE? should i chuck them?
also i will be using these for my first monotub attempt. done a shitload of research and plan on doing a coir/hpoo/verm mix. if anyone knwo a good mono tek with these ingredients or have any suggestions i would really appreciate it.
sorry if this a stupid question and thanks in advance!
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LumpyNutz
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Registered: 07/14/14
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Re: WBS topper? (lid) [Re: LumpyNutz]
#22463944 - 11/01/15 07:36 PM (8 years, 2 months ago) |
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btw i have the tyvek (sp?) envelope stuff cut in circles under the lids as well. thanks for any input
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Psilosoulful

Registered: 09/05/14
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Re: WBS topper? (lid) [Re: LumpyNutz]
#22464024 - 11/01/15 07:53 PM (8 years, 2 months ago) |
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Quote:
LumpyNutz said: But my question is: Do I keepp the 2 coffee filters off the top after sterilization/innoc?? I got the lid under them with the 1/4" hole stuffed with polyfill so im wondering is the coffee filters necessary after innoc?? will they reduce FAE? should i chuck them?
Yeah take that off, it'll restrict the GE. You should have the polyfill tightly stuffed in the hole, this will ward off potential contams. Just make sure it stays dry.
Quote:
LumpyNutz said: also i will be using these for my first monotub attempt. done a shitload of research and plan on doing a coir/hpoo/verm mix. if anyone knwo a good mono tek with these ingredients or have any suggestions i would really appreciate it.
I would use 40% coir/ 40% hpoo/ 20% verm for the bulk sub. And if you have garden gypsum available, add a couple of handfuls of that.
Make sure you bring it to field capacity, load up your jars, and properly pasteurize the mix for 1 hour at 140-165f. Here's a great tek for pasteurizing procedures: http://www.shroomery.org/forums/showflat.php/Number/17246844/fpart/all/vc/1
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LumpyNutz
Bad fisH <*))))<



Registered: 07/14/14
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Hey thanks much for the info bro.gonna take them filters off right now. And thanks for the tek will deffinTely be checKing that out.
Also I accidentally shook my jars every day for the first three days. I thought that's how u supposed to do it. .. so I'm like 5 or 6 days in and no sign of mycelium.
[url=https://files.shroomery.org/files/15-45/650260875-20151102_171259.jpg] [/url So here's how they look atm they look ok?
Thanks
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Machiavelliavore
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Re: WBS topper? (lid) [Re: LumpyNutz]
#22467142 - 11/02/15 03:41 PM (8 years, 2 months ago) |
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The tyvek filters alone would be enough but for the holes you punctured in them with the syringe. So yeah, all the poly has to do is cover a pinhole. Even if it's not stuffed as tightly as it should be, you should be fine. I doubt the coffee filters did much of anything.
6-8 days is typical for germination. Not sure if that even holds for PE. Up to like 3 weeks is possible though uncommon.
By shaking, you've distributed the spores all over, so it will be harder to see germination, and it will most likely be more diffuse and harder to observe. Additionally, you may have damaged fragile microscopic mycellium that just germination by shaking, further slowing it.
Still, no cause for concern.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Arush
Godfather



Registered: 05/06/13
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coffee filters pretty much do nothing.
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LumpyNutz
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Registered: 07/14/14
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Re: WBS topper? (lid) [Re: Arush]
#22469869 - 11/03/15 07:37 AM (8 years, 2 months ago) |
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IM CONCERNED: my wbs jars have been about 6 days now and still no sign of mycelium.. so im wondering; would adding 1ml~ more of spores be a good idea? i got 1/2 a syringe of PE left i was planning on using for more jars, but i feel i might have fucked my grow by shaking the first 3 days and maybe i should just re-noc'.. i might be paranoid/anxious, but what do u guys think?
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azur
God of Fuck



Registered: 04/21/12
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Re: WBS topper? (lid) [Re: LumpyNutz]
#22469888 - 11/03/15 07:45 AM (8 years, 2 months ago) |
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Spores to grain can take 2 to 3 weeks before you see anything. You've been here long enough. Why aren't you using agar yet?
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Psilosoulful

Registered: 09/05/14
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Re: WBS topper? (lid) [Re: azur]
#22469930 - 11/03/15 08:04 AM (8 years, 2 months ago) |
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Quote:
azur said: You've been here long enough. Why aren't you using agar yet?
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tetherface
get in where you fit in



Registered: 10/05/14
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Re: WBS topper? (lid) [Re: LumpyNutz]
#22470103 - 11/03/15 09:05 AM (8 years, 2 months ago) |
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Quote:
LumpyNutz said: IM CONCERNED: my wbs jars have been about 6 days now and still no sign of mycelium.. so im wondering; would adding 1ml~ more of spores be a good idea? i got 1/2 a syringe of PE left i was planning on using for more jars, but i feel i might have fucked my grow by shaking the first 3 days and maybe i should just re-noc'.. i might be paranoid/anxious, but what do u guys think?
no need for more spores patience is key bro 6 days is nothing as it could take 2-3 weeks but you could just make up more grain jars to inoculate if your that concerned about it. IMO the initial shaking didn't sink yer battleship but may have slowed you down a few days good luck
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DrCrumbs
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Registered: 10/25/11
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Yeah after coming back recently I needed to start with spores. I should have started on agar. I mean I did, I did agar and grains at the same time, I shouldnt have bothered with the grains.
One grain set took Ten days before signs (PESA from print 2012), a Second Two/Three weeks (PESH), and still waiting (B+).
I think the speed was more due to the age of the spores than the strain. I think popularity is a huge role in this. When a var. is popular and being bought up and used they tend to be fresher. B+ used to be super fast from MS.
The agar lapped a lot of them. Even when signs were shown on grain it still took longer to colonize.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: WBS topper? (lid) [Re: DrCrumbs]
#22470719 - 11/03/15 12:00 PM (8 years, 2 months ago) |
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Yea once you start using grains a clean culture is a must. Grains always should start with agar.
Why are you using both tyvek and poly? One or the other man. I use 4 1/4 inch holes at the corners of the lid kinda like a PF tek lid, then cover with a layer of tyvek between lid and band. Or a couple layers of Kraft Felt. If Im using poly Ill use one big hole and stuff it tight as possible but poly isn't my filter of choice because it can get pushed thru.
These days I use synthetic filter discs and plastic autoclaveable lids. Here's a photo of felt lids and SFDs
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LumpyNutz
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Registered: 07/14/14
Posts: 712
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Re: WBS topper? (lid) [Re: mushpunx]
#22474993 - 11/04/15 08:35 AM (8 years, 2 months ago) |
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Quote:
Psilosoulful said:
Quote:
azur said: You've been here long enough. Why aren't you using agar yet?
haha ya ive heard a lot of good things about agar but never really looked into it. i moved 3 times in the last year sooo been kinda inconsistant and slacking on my research >.< thanks for the tip tho any1 got a good agar to grain tek? ima search for one but if someone got a good one thatd be sweet.
Quote:
mushpunx said:

Yea once you start using grains a clean culture is a must. Grains always should start with agar.
Why are you using both tyvek and poly? One or the other man. I use 4 1/4 inch holes at the corners of the lid kinda like a PF tek lid, then cover with a layer of tyvek between lid and band. Or a couple layers of Kraft Felt. If Im using poly Ill use one big hole and stuff it tight as possible but poly isn't my filter of choice because it can get pushed thru.
These days I use synthetic filter discs and plastic autoclaveable lids. Here's a photo of felt lids and SFDs
ay thanks for the polly + tyvek tip man on Doc's tek it says to put a layer of tyvex first, then one 1/4" hole with polly on the lid so thats how i did. u think that might be reducing tthe fae too much? remove the polly from the lid maybe?
oh yea and THANKS i was under the impression that grains, however the spawn input method, takes the same time, so i dont feel so anxious knowing using spore syringe take quite a bit longer to consol! props yall u guys r great!
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mushpunx
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Re: WBS topper? (lid) [Re: LumpyNutz]
#22476151 - 11/04/15 11:55 AM (8 years, 2 months ago) |
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There is no FAE in jars, the filter is for Gas Exchange (GE).
At this point I wpuld leave the lids alone and next time use one or the other
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