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OfflineIngSocTHC
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KSSS growth
    #22463710 - 11/01/15 06:46 PM (8 years, 2 months ago)

I was wondering if this growth looked normal. I have not seen what looks like knotting right off the bat. Also the (pregrowth?) Looks whispy and grey. I'm sure it's all fine, just checking.


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OfflineIngSocTHC
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Re: KSSS growth [Re: IngSocTHC]
    #22463716 - 11/01/15 06:48 PM (8 years, 2 months ago)

No bong :-) learning how to do this shot from the phone, small line tricky


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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OfflineIngSocTHC
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Re: KSSS growth [Re: IngSocTHC]
    #22463749 - 11/01/15 06:52 PM (8 years, 2 months ago)

Oh and these were knocked up MS in SAB w/proper technique on 10/26/15.


--------------------
"Music your, music your the key.
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Bring the voice of the rastaman
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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: IngSocTHC]
    #22463770 - 11/01/15 06:56 PM (8 years, 2 months ago)

Looks like mold


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OfflinePsilosoulful

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Re: KSSS growth [Re: IngSocTHC]
    #22463808 - 11/01/15 07:06 PM (8 years, 2 months ago)

Quote:

IngSocTHC said:
Oh and these were knocked up MS in SAB w/proper technique on 10/26/15.



I agree with flabber, that is definitely mold. If your techniques in the SAB were good, then your syringe is most likely the reason for the contam.


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OfflineFunguy24
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Re: KSSS growth [Re: IngSocTHC]
    #22463809 - 11/01/15 07:07 PM (8 years, 2 months ago)

i would say that's definitely mold :frown: what are you growing? how did you prepare the spawn?


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OfflinePsilosoulful

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Re: KSSS growth [Re: Funguy24]
    #22463822 - 11/01/15 07:10 PM (8 years, 2 months ago)

Dude.. read the title of the thread :lol:


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OfflineIngSocTHC
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Re: KSSS growth [Re: Funguy24]
    #22463825 - 11/01/15 07:11 PM (8 years, 2 months ago)

I mentioned KSSS earlier, I prep with a overnight soak, bring to a solid boil and aor dry, load jars PC in my AA921 for 2hrs (half gal jars). The areas the growth is appearing is where I injected the 2cc of MS.


--------------------
"Music your, music your the key.
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Bring the voice of the rastaman
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OfflinePsilosoulful

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Re: KSSS growth [Re: IngSocTHC]
    #22463844 - 11/01/15 07:15 PM (8 years, 2 months ago)

You injected too much solution, which probably offset the moisture content of the grains, making them more susceptible to bacterial contams. Next time use between 0.5-1cc .


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OfflineIngSocTHC
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Re: KSSS growth [Re: Psilosoulful]
    #22463851 - 11/01/15 07:17 PM (8 years, 2 months ago)

There is no standing water.


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"Music your, music your the key.
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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: Psilosoulful]
    #22463855 - 11/01/15 07:17 PM (8 years, 2 months ago)

You might need to PC longer if using half gallon jars.


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InvisibleKalistis
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Re: KSSS growth [Re: IngSocTHC]
    #22463856 - 11/01/15 07:18 PM (8 years, 2 months ago)

Looks kinda like yeast to me. :shrug:


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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: Psilosoulful]
    #22463859 - 11/01/15 07:19 PM (8 years, 2 months ago)

Quote:

Psilosoulful said:
You injected too much solution, which probably offset the moisture content of the grains, making them more susceptible to bacterial contams. Next time use between 0.5-1cc .



He could put the whole syringe in there and it wouldn't offset the moisture content enough to matter.


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OfflineIngSocTHC
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Re: KSSS growth [Re: wowimflabbergasted]
    #22463870 - 11/01/15 07:21 PM (8 years, 2 months ago)

Quote:

wowimflabbergasted said:
You might need to PC longer if using half gallon jars.




The growth is showing only where the injection happens. The other 6 half gallon jars that I haven't nocced up shows no growth.


--------------------
"Music your, music your the key.
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Bring the voice of the rastaman
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OfflinePsilosoulful

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Re: KSSS growth [Re: wowimflabbergasted]
    #22463880 - 11/01/15 07:23 PM (8 years, 2 months ago)

Quote:

wowimflabbergasted said:
Quote:

Psilosoulful said:
You injected too much solution, which probably offset the moisture content of the grains, making them more susceptible to bacterial contams. Next time use between 0.5-1cc .



He could put the whole syringe in there and it wouldn't offset the moisture content enough to matter.



Depends where he injected the solution. If it was directly in the center of the jar, the surrounding grains would absorb the excess moisture.

If he injected against the glass, you would have a bunch of wet grains stuck to the side of the glass. Over time, if there was bacteria present in the syringe, it could easily thrive at that location. And given the look of the jars, it looks like that was the case.


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OfflineIngSocTHC
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Re: KSSS growth [Re: Psilosoulful]
    #22463889 - 11/01/15 07:24 PM (8 years, 2 months ago)

Also, got the spores from TSD and used them hr after they arrived.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: IngSocTHC]
    #22463897 - 11/01/15 07:26 PM (8 years, 2 months ago)

What excess moisture?? 2ml isn't excess. 100ml is excess....and still works :lol:


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InvisibleKalistis
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Re: KSSS growth [Re: wowimflabbergasted]
    #22463909 - 11/01/15 07:28 PM (8 years, 2 months ago)

I'm not sure if excessive is the right word, but completely unnecessary. I have used as little as 0.5 ml per quart with great colonization times.


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OfflineIngSocTHC
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Re: KSSS growth [Re: Kalistis]
    #22463929 - 11/01/15 07:34 PM (8 years, 2 months ago)

Also I had some B+ look the same way all the way up to 100% colonization, now looks cherry.


--------------------
"Music your, music your the key.
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Bring the voice of the rastaman
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OfflinePsilosoulful

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Re: KSSS growth [Re: IngSocTHC]
    #22463956 - 11/01/15 07:39 PM (8 years, 2 months ago)

OP I hope you get into agar soon, you can completely avoid these issues if you start with a clean culture.


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OfflineIngSocTHC
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Re: KSSS growth [Re: Psilosoulful]
    #22463975 - 11/01/15 07:42 PM (8 years, 2 months ago)

I have all the agar setup, just still getting it dialed in. First round I got 0 growth for almost a month so I made it less stiff...little too soft and now going to try again but also was hoping to get a good G2G started to isolate from.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: IngSocTHC]
    #22463985 - 11/01/15 07:45 PM (8 years, 2 months ago)

Don't think that's gunna happen bud. Keep at agar and you'll get it in no time though.


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OfflineDeceivedrelico
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Re: KSSS growth [Re: wowimflabbergasted]
    #22463990 - 11/01/15 07:46 PM (8 years, 2 months ago)

Coool


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InvisibleHigher Love
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Re: KSSS growth [Re: Psilosoulful]
    #22463995 - 11/01/15 07:46 PM (8 years, 2 months ago)


Start of my KSSS. From MS to Agar to Rye
Hope this helps


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OfflinefirstTIMER420
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Re: KSSS growth [Re: IngSocTHC]
    #22464495 - 11/01/15 09:35 PM (8 years, 2 months ago)

Quote:

IngSocTHC said:
I have all the agar setup, just still getting it dialed in. First round I got 0 growth for almost a month so I made it less stiff...little too soft and now going to try again but also was hoping to get a good G2G started to isolate from.




Don't know what recipe you are using but MEA and PDA are the easiest and most common.  Those jars in the first post are straight mold.  Don't worry though, happened to me(and everyone) as well.

Here is a valuable link on proper recipes. Agar recipe handook

For reference, its 10/10/500ml; that is, 10mg agar, 10mg LME, 500ml water, enough to pour 20- 100x15mm plates

But remember with agar, you want to PC for about 30-45 minutes, have a cap with a hole and an SFD stuffed in it.  Pour when the temp is at 125-135.  The longer you wait the less condensation.  I waited till it was about 120 last time, and I got 0 condensation. Don't let your hand over the plate.  Also, twist the bottle at the end of each pour, and you don't need to fill up the entire plate, just enough to cover the bottom.

If you have any more questions, :message: me

good luck!


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12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
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OfflineIngSocTHC
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Re: KSSS growth [Re: Higher Love]
    #22464501 - 11/01/15 09:36 PM (8 years, 2 months ago)

I don't get how every question always leads back to agar. I mean I understand that agar is the shot, but not too long ago people successfully grew never doing agar. Now it seems every ones outlook is agar or fail.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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OfflinecronicrFacebook
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Re: KSSS growth [Re: IngSocTHC]
    #22464512 - 11/01/15 09:39 PM (8 years, 2 months ago)

It has always been this way.....don't see that changing


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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: IngSocTHC]
    #22464515 - 11/01/15 09:40 PM (8 years, 2 months ago)

Not the case really. It's more like if you fail or keep failing, try agar. :wink:


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OfflinefirstTIMER420
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Re: KSSS growth [Re: IngSocTHC]
    #22464542 - 11/01/15 09:45 PM (8 years, 2 months ago)

Not really agar or fail.  Its just that agar makes it so you are 100% sure your not wasting your time.  Ive only been cultivating for 4 months. Look at my sig, those tubs are because of agar.

I just got into agar 2 months ago, because everyone was saying the same thing lol, but now that I have started, its the best part about mycology.

People have always been doing agar, its just not getting to where everyone is able to do it.

As I said, you can grow successfully, and there are people who never touch agar and grow for years, but its always random.  I was doing cakes and then had an entire batch get trich, I got pissed and said never again will I use MS syringes to inoculate, and got into agar immediately after that.


Havent looked back



btw, the other link is broken,this is the proper one


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InvisibleHigher Love
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Re: KSSS growth [Re: IngSocTHC]
    #22464550 - 11/01/15 09:46 PM (8 years, 2 months ago)

Quote:

IngSocTHC said:
I don't get how every question always leads back to agar. I mean I understand that agar is the shot, but not too long ago people successfully grew never doing agar. Now it seems every ones outlook is agar or fail.




Agar simply can provide better consistency. This is obvious. But we all know it's not a necessity... plus, it's fun and saves product in the long run.

P.s. my post above with the pic was to show u that my ksss grow was also wispy looking, in hopes to provide u some comfort that yours was on a normal track (ur original concern).
I have been isolating KSSS strains on agar for quite some time now and they all seem wispy and thinnish, compared to many others at least. Maybe the unluck of the draw with that syringe but in the future maybe it will be different. I shall keep trying


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OfflineFunguy24
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Re: KSSS growth [Re: Psilosoulful]
    #22465076 - 11/02/15 12:25 AM (8 years, 2 months ago)

never seen the abbreviation before SORRY and by how did you prepare i mean exactly how, one persons idea of a sterile technique may differ from anothers.


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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: Higher Love]
    #22465142 - 11/02/15 12:53 AM (8 years, 2 months ago)

Quote:

Higher Love said:
Quote:

IngSocTHC said:
I don't get how every question always leads back to agar. I mean I understand that agar is the shot, but not too long ago people successfully grew never doing agar. Now it seems every ones outlook is agar or fail.




Agar simply can provide better consistency. This is obvious. But we all know it's not a necessity... plus, it's fun and saves product in the long run.

P.s. my post above with the pic was to show u that my ksss grow was also wispy looking, in hopes to provide u some comfort that yours was on a normal track (ur original concern).
I have been isolating KSSS strains on agar for quite some time now and they all seem wispy and thinnish, compared to many others at least. Maybe the unluck of the draw with that syringe but in the future maybe it will be different. I shall keep trying



Not sure what you're looking at but OP's is growing mold, probably cobweb, sorry. :shrug:


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OfflineIngSocTHC
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Re: KSSS growth [Re: firstTIMER420]
    #22465228 - 11/02/15 01:59 AM (8 years, 2 months ago)

Quote:

firstTIMER420 said:
Quote:

IngSocTHC said:
I have all the agar setup, just still getting it dialed in. First round I got 0 growth for almost a month so I made it less stiff...little too soft and now going to try again but also was hoping to get a good G2G started to isolate from.




Don't know what recipe you are using but MEA and PDA are the easiest and most common.  Those jars in the first post are straight mold.  Don't worry though, happened to me(and everyone) as well.

Here is a valuable link on proper recipes. Agar recipe handook

For reference, its 10/10/500ml; that is, 10mg agar, 10mg LME, 500ml water, enough to pour 20- 100x15mm plates

But remember with agar, you want to PC for about 30-45 minutes, have a cap with a hole and an SFD stuffed in it.  Pour when the temp is at 125-135.  The longer you wait the less condensation.  I waited till it was about 120 last time, and I got 0 condensation. Don't let your hand over the plate.  Also, twist the bottle at the end of each pour, and you don't need to fill up the entire plate, just enough to cover the bottom.

If you have any more questions, :message: me

good luck!




I'm useing a premix PDA from amazon with glad mini rounds following pastywhites no pour agar tek. I'm down for doing pour then PC but PCing the whole container seems more logical because everything gets sterilized. Also how are you guys reading the temp on the agar?


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: IngSocTHC]
    #22465596 - 11/02/15 07:32 AM (8 years, 2 months ago)

Everything in the PC gets sterilized doing both pour or no pour...except plastic petris which already come sterile.


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Re: KSSS growth [Re: wowimflabbergasted]
    #22465747 - 11/02/15 08:17 AM (8 years, 2 months ago)





Not sure what you're looking at but OP's is growing mold, probably cobweb, sorry. :shrug:




I'm not sure what I was looking at either honestly. I am sorry for the uncanny and misleading reply gentlemen.


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Invisiblewowimflabbergasted
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Re: KSSS growth [Re: Higher Love]
    #22466170 - 11/02/15 10:51 AM (8 years, 2 months ago)

All good :lol:


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OfflineIngSocTHC
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Re: KSSS growth [Re: wowimflabbergasted]
    #22467360 - 11/02/15 04:41 PM (8 years, 2 months ago)

What are you useing to measure the temp and best place to get the Y-segment agar plates? Also I've been battling with bacteria more than mold, and from my few experiments with agar it seems difficult to spot. Amy tips?


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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OfflinefirstTIMER420
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Re: KSSS growth [Re: IngSocTHC]
    #22467780 - 11/02/15 06:17 PM (8 years, 2 months ago)

I use a temperature gun.  Bought at home depot. about 20 bucks, works like a charm.


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OfflineIngSocTHC
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Re: KSSS growth [Re: firstTIMER420]
    #22467794 - 11/02/15 06:19 PM (8 years, 2 months ago)

Cheeeee! Thanks bro.


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Talk to who, plese talk to me.
Bring the voice of the rastaman
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