Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineIngSocTHC
 User Gallery


Registered: 07/06/05
Posts: 281
Last seen: 7 years, 11 months
Re: KSSS growth [Re: Psilosoulful]
    #22463975 - 11/01/15 07:42 PM (8 years, 2 months ago)

I have all the agar setup, just still getting it dialed in. First round I got 0 growth for almost a month so I made it less stiff...little too soft and now going to try again but also was hoping to get a good G2G started to isolate from.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: KSSS growth [Re: IngSocTHC]
    #22463985 - 11/01/15 07:45 PM (8 years, 2 months ago)

Don't think that's gunna happen bud. Keep at agar and you'll get it in no time though.


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
OfflineDeceivedrelico
Stranger
Registered: 11/01/15
Posts: 1
Last seen: 8 years, 2 months
Re: KSSS growth [Re: wowimflabbergasted]
    #22463990 - 11/01/15 07:46 PM (8 years, 2 months ago)

Coool


Extras: Filter Print Post Top
InvisibleHigher Love
Envisioneer
Male User Gallery


Registered: 09/24/15
Posts: 384
Loc: PNW
Re: KSSS growth [Re: Psilosoulful]
    #22463995 - 11/01/15 07:46 PM (8 years, 2 months ago)


Start of my KSSS. From MS to Agar to Rye
Hope this helps


--------------------


Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: KSSS growth [Re: IngSocTHC]
    #22464495 - 11/01/15 09:35 PM (8 years, 2 months ago)

Quote:

IngSocTHC said:
I have all the agar setup, just still getting it dialed in. First round I got 0 growth for almost a month so I made it less stiff...little too soft and now going to try again but also was hoping to get a good G2G started to isolate from.




Don't know what recipe you are using but MEA and PDA are the easiest and most common.  Those jars in the first post are straight mold.  Don't worry though, happened to me(and everyone) as well.

Here is a valuable link on proper recipes. Agar recipe handook

For reference, its 10/10/500ml; that is, 10mg agar, 10mg LME, 500ml water, enough to pour 20- 100x15mm plates

But remember with agar, you want to PC for about 30-45 minutes, have a cap with a hole and an SFD stuffed in it.  Pour when the temp is at 125-135.  The longer you wait the less condensation.  I waited till it was about 120 last time, and I got 0 condensation. Don't let your hand over the plate.  Also, twist the bottle at the end of each pour, and you don't need to fill up the entire plate, just enough to cover the bottom.

If you have any more questions, :message: me

good luck!


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Extras: Filter Print Post Top
OfflineIngSocTHC
 User Gallery


Registered: 07/06/05
Posts: 281
Last seen: 7 years, 11 months
Re: KSSS growth [Re: Higher Love]
    #22464501 - 11/01/15 09:36 PM (8 years, 2 months ago)

I don't get how every question always leads back to agar. I mean I understand that agar is the shot, but not too long ago people successfully grew never doing agar. Now it seems every ones outlook is agar or fail.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: KSSS growth [Re: IngSocTHC]
    #22464512 - 11/01/15 09:39 PM (8 years, 2 months ago)

It has always been this way.....don't see that changing


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: KSSS growth [Re: IngSocTHC]
    #22464515 - 11/01/15 09:40 PM (8 years, 2 months ago)

Not the case really. It's more like if you fail or keep failing, try agar. :wink:


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: KSSS growth [Re: IngSocTHC]
    #22464542 - 11/01/15 09:45 PM (8 years, 2 months ago)

Not really agar or fail.  Its just that agar makes it so you are 100% sure your not wasting your time.  Ive only been cultivating for 4 months. Look at my sig, those tubs are because of agar.

I just got into agar 2 months ago, because everyone was saying the same thing lol, but now that I have started, its the best part about mycology.

People have always been doing agar, its just not getting to where everyone is able to do it.

As I said, you can grow successfully, and there are people who never touch agar and grow for years, but its always random.  I was doing cakes and then had an entire batch get trich, I got pissed and said never again will I use MS syringes to inoculate, and got into agar immediately after that.


Havent looked back



btw, the other link is broken,this is the proper one


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Extras: Filter Print Post Top
InvisibleHigher Love
Envisioneer
Male User Gallery


Registered: 09/24/15
Posts: 384
Loc: PNW
Re: KSSS growth [Re: IngSocTHC]
    #22464550 - 11/01/15 09:46 PM (8 years, 2 months ago)

Quote:

IngSocTHC said:
I don't get how every question always leads back to agar. I mean I understand that agar is the shot, but not too long ago people successfully grew never doing agar. Now it seems every ones outlook is agar or fail.




Agar simply can provide better consistency. This is obvious. But we all know it's not a necessity... plus, it's fun and saves product in the long run.

P.s. my post above with the pic was to show u that my ksss grow was also wispy looking, in hopes to provide u some comfort that yours was on a normal track (ur original concern).
I have been isolating KSSS strains on agar for quite some time now and they all seem wispy and thinnish, compared to many others at least. Maybe the unluck of the draw with that syringe but in the future maybe it will be different. I shall keep trying


--------------------


Extras: Filter Print Post Top
OfflineFunguy24
Stranger

Registered: 11/01/15
Posts: 33
Last seen: 1 year, 5 months
Re: KSSS growth [Re: Psilosoulful]
    #22465076 - 11/02/15 12:25 AM (8 years, 2 months ago)

never seen the abbreviation before SORRY and by how did you prepare i mean exactly how, one persons idea of a sterile technique may differ from anothers.


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: KSSS growth [Re: Higher Love]
    #22465142 - 11/02/15 12:53 AM (8 years, 2 months ago)

Quote:

Higher Love said:
Quote:

IngSocTHC said:
I don't get how every question always leads back to agar. I mean I understand that agar is the shot, but not too long ago people successfully grew never doing agar. Now it seems every ones outlook is agar or fail.




Agar simply can provide better consistency. This is obvious. But we all know it's not a necessity... plus, it's fun and saves product in the long run.

P.s. my post above with the pic was to show u that my ksss grow was also wispy looking, in hopes to provide u some comfort that yours was on a normal track (ur original concern).
I have been isolating KSSS strains on agar for quite some time now and they all seem wispy and thinnish, compared to many others at least. Maybe the unluck of the draw with that syringe but in the future maybe it will be different. I shall keep trying



Not sure what you're looking at but OP's is growing mold, probably cobweb, sorry. :shrug:


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
OfflineIngSocTHC
 User Gallery


Registered: 07/06/05
Posts: 281
Last seen: 7 years, 11 months
Re: KSSS growth [Re: firstTIMER420]
    #22465228 - 11/02/15 01:59 AM (8 years, 2 months ago)

Quote:

firstTIMER420 said:
Quote:

IngSocTHC said:
I have all the agar setup, just still getting it dialed in. First round I got 0 growth for almost a month so I made it less stiff...little too soft and now going to try again but also was hoping to get a good G2G started to isolate from.




Don't know what recipe you are using but MEA and PDA are the easiest and most common.  Those jars in the first post are straight mold.  Don't worry though, happened to me(and everyone) as well.

Here is a valuable link on proper recipes. Agar recipe handook

For reference, its 10/10/500ml; that is, 10mg agar, 10mg LME, 500ml water, enough to pour 20- 100x15mm plates

But remember with agar, you want to PC for about 30-45 minutes, have a cap with a hole and an SFD stuffed in it.  Pour when the temp is at 125-135.  The longer you wait the less condensation.  I waited till it was about 120 last time, and I got 0 condensation. Don't let your hand over the plate.  Also, twist the bottle at the end of each pour, and you don't need to fill up the entire plate, just enough to cover the bottom.

If you have any more questions, :message: me

good luck!




I'm useing a premix PDA from amazon with glad mini rounds following pastywhites no pour agar tek. I'm down for doing pour then PC but PCing the whole container seems more logical because everything gets sterilized. Also how are you guys reading the temp on the agar?


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: KSSS growth [Re: IngSocTHC]
    #22465596 - 11/02/15 07:32 AM (8 years, 2 months ago)

Everything in the PC gets sterilized doing both pour or no pour...except plastic petris which already come sterile.


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
InvisibleHigher Love
Envisioneer
Male User Gallery


Registered: 09/24/15
Posts: 384
Loc: PNW
Re: KSSS growth [Re: wowimflabbergasted]
    #22465747 - 11/02/15 08:17 AM (8 years, 2 months ago)





Not sure what you're looking at but OP's is growing mold, probably cobweb, sorry. :shrug:




I'm not sure what I was looking at either honestly. I am sorry for the uncanny and misleading reply gentlemen.


--------------------


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: KSSS growth [Re: Higher Love]
    #22466170 - 11/02/15 10:51 AM (8 years, 2 months ago)

All good :lol:


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
OfflineIngSocTHC
 User Gallery


Registered: 07/06/05
Posts: 281
Last seen: 7 years, 11 months
Re: KSSS growth [Re: wowimflabbergasted]
    #22467360 - 11/02/15 04:41 PM (8 years, 2 months ago)

What are you useing to measure the temp and best place to get the Y-segment agar plates? Also I've been battling with bacteria more than mold, and from my few experiments with agar it seems difficult to spot. Amy tips?


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: KSSS growth [Re: IngSocTHC]
    #22467780 - 11/02/15 06:17 PM (8 years, 2 months ago)

I use a temperature gun.  Bought at home depot. about 20 bucks, works like a charm.


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Extras: Filter Print Post Top
OfflineIngSocTHC
 User Gallery


Registered: 07/06/05
Posts: 281
Last seen: 7 years, 11 months
Re: KSSS growth [Re: firstTIMER420]
    #22467794 - 11/02/15 06:19 PM (8 years, 2 months ago)

Cheeeee! Thanks bro.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Penis Envy & KSSS - how hard is it to create new spawns? Nocforweb 2,847 4 08/02/17 01:36 AM
by B-nizzle
* Slow growth psilocybe cubensis, pftek Cherie 1,076 11 05/06/21 08:50 AM
by tiptrippy
* request for KSSS pics trentallica 3,396 10 05/15/06 06:56 PM
by monstermitch
* very slow, clear growth on agar *DELETED*
( 1 2 all )
psh 1,841 22 05/21/21 02:47 PM
by Mycelium Juice
* Growth stalled whitebuffalo 225 0 05/05/21 02:49 AM
by whitebuffalo
* Finally, Some KSSS that look like Shrooms HippieChick 4,042 19 03/26/05 10:19 AM
by hammer_head
* KSSS on PF cakes? Ferment 1,630 7 11/16/04 10:17 PM
by aoxomoxoaMan
* KSSS thremix 5,942 7 08/16/05 07:44 AM
by FooMan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,942 topic views. 39 members, 245 guests and 41 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.008 seconds on 14 queries.