|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 18 hours, 8 minutes
|
Re: contamination question [Re: tedoro]
#22604537 - 12/02/15 03:05 PM (8 years, 1 month ago) |
|
|
I did a transfer of the plate seen above, and it didn't go well. It feels like mold, not mycelia. It disappeared when I scraped it, unlike the mycelia I have done transfers with in the past, which collapsed into a little white thread-like clump.
I'll grow out these two samples I have, but its not looking good.
t
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: contamination question [Re: tedoro]
#22604544 - 12/02/15 03:06 PM (8 years, 1 month ago) |
|
|
Quote:
tedoro said: Is there anything wrong with using these agar plates?
http://www.amazon.com/gp/product/B004MKHNJK?psc=1&redirect=true&ref_=oh_aui_detailpage_o06_s00
they're pre-poured. you don't want that. shipping un-colonized un-inoculated agar is pretty dumb. I would say at least 1/4 of the time people order pre-pour dishes they come half cracked, or half contaminated.
pouring agar into a petri dish is by far the easiest thing in all of growing mushrooms besides maybe hydrating a brick of coir.
it would cost less money to buy a packet of agar agar and sterile dishes then it would be to order the pre-pour.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: contamination question [Re: bodhisatta]
#22604616 - 12/02/15 03:18 PM (8 years, 1 month ago) |
|
|
That media is meant for bacteria not fungi. Learn to make your own agar, you will see better results. That price is a ripoff as well.
Also don't worry about ropey vs cottony growth. Worry about clean healthy growth. Some of my best performers are cottony (tomentose).
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: contamination question [Re: Pastywhyte]
#22604654 - 12/02/15 03:24 PM (8 years, 1 month ago) |
|
|
Quote:
Pastywhyte said: That media is meant for bacteria not fungi. Learn to make your own agar, you will see better results. That price is a ripoff as well.
Also don't worry about ropey vs cottony growth. Worry about clean healthy growth. Some of my best performers are cottony (tomentose).


cottony(tomentose) turns to ropey(rhizo) and vice versa. it really is more dependent on nutrition, temperature, time. it's not really any indication of performance.
http://www.shroomery.org/forums/usergallery.php/gallery/300678/imgpl/5/imgpp/100/folder/agar
Edited by Trusted cuItivator (12/02/15 03:30 PM)
|
tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 18 hours, 8 minutes
|
Re: contamination question [Re: bodhisatta]
#22604938 - 12/02/15 04:14 PM (8 years, 1 month ago) |
|
|
bodhisatta...
That second dish on the right... it has growth that is cottony and a bit greyish, with ropey growth coming out of the top. Whats going on there? Is that all mycelia?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
|
tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 18 hours, 8 minutes
|
Re: contamination question [Re: tedoro]
#22604958 - 12/02/15 04:21 PM (8 years, 1 month ago) |
|
|
I was pouring my own agar dishes in a still air box and I found it very awkward. Considering the time and expense of everything else, I just thought I could buy my way out of the part I disliked the most. None of them have grown any contaminants. How are these my pre-poured agar dishes different than the dried agar I purchased and mixed at home? Is there specific mushroom agar? Maybe this is where I'm going wrong.
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
|
tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 18 hours, 8 minutes
|
Re: contamination question [Re: tedoro]
#22604967 - 12/02/15 04:23 PM (8 years, 1 month ago) |
|
|
and are those transferred chunks of agar placed in the new dish upside down to make contact with the new agar?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: contamination question [Re: tedoro]
#22604981 - 12/02/15 04:27 PM (8 years, 1 month ago) |
|
|
Those dishes have the wrong nutrients for fungi. Agar is agar but what you use as nutrient is important. Those plates are luria broth which has no carbs or sugars, which is what fungi primarily need. Also Presterilized shit is notorious for being damaged and/or contamed in shipping.
If you don't like pouring agar then try no pour. It works pretty good IMO.
|
tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 18 hours, 8 minutes
|
Re: contamination question [Re: Pastywhyte]
#22604996 - 12/02/15 04:31 PM (8 years, 1 month ago) |
|
|
Wait, so agar powder is actually a mixture of agar and some level of nutrients? I thought the agar was a low level nutrient.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: contamination question [Re: tedoro]
#22605013 - 12/02/15 04:35 PM (8 years, 1 month ago) |
|
|
Agar is made from seaweed and has zero nutrients. We then add nutrients to it. MEA = malt extract agar. PDA = Potato dextrose agar. DFA = Dog food agar. Etc.
Many people buy their agar powder with nutrients already mixed in. I personally like to make my own mixes, that allows me to change recipes on the fly which can provide vigor to a lazy culture. It also helps to ward off senscence.
|
tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 18 hours, 8 minutes
|
Re: contamination question [Re: Pastywhyte]
#22605041 - 12/02/15 04:41 PM (8 years, 1 month ago) |
|
|
I am soooo excited! This is what I needed to hear... I bet this is causing my troubles! And now I think these recent plates of mine aren't contaminated.
I think I will move forward with them, and try the no-pour dishes.
Many many thanks.
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: contamination question [Re: tedoro]
#22605170 - 12/02/15 05:18 PM (8 years, 1 month ago) |
|
|
Quote:
tedoro said: bodhisatta...
That second dish on the right... it has growth that is cottony and a bit greyish, with ropey growth coming out of the top. Whats going on there? Is that all mycelia?
It's all mycelium. Where it turns in the top to ropey just goes to show again that tomentose / rhizo. Doesn't matter. Healthy growth matters.
Quote:
tedoro said: and are those transferred chunks of agar placed in the new dish upside down to make contact with the new agar?
No they land either way I don't make an effort to have them face one way or the other.
|
|