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rudeboi
Noob

Registered: 05/17/14
Posts: 107
Loc: North BC
Last seen: 5 months, 13 days
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Quote:
Arctic W. Fox said:
- 12 wide-mouth half-pint jars.
- Four holes drilled in the lids, equally spaced.
- Mixed 1-part water, 1-part organic BRF, 3-parts Verm.
- 1/2 cup of fluffed mixture in each jar, 4tsp of dry Verm on top.
- Placed lid upside-down and secured on jars.
- Cut aluminium foil to size and covered the lid.
- Place jars inside PC, stacked two high.
- Placed open spring-water-filled pickle jar in PC to space BRF jars.
- Filled PC with spring water about half way up bottom BRF jars.
- Closed PC, added 15psi weights, turned on heat to high until steam, brought down heat until the familiar pst...pst...pst...
- Pressure cook for 2 hours. Turn off heat. Let set for 24 hours.
- Remove weights, remove PC lid.
- Syringe at room temperature and shaken (not stirred).
- Carefully remove foil from one jar, flame sterilize needle with lighter, wait 10 seconds to cool, inoculate through each drilled holes at full needle depth with about 1/4cc (almost 1cc of fluid per jar). Replaced foil. Set inside cabinet shelf.
- Repeat.
Any fluid left in the syringe at the end of 12 jars gets dumped into the last jar.
I check back a week later and find nothing useful growing and have to toss unopened/contaminated jars.
I have done this five other times with 100% success. These last three times, I got nada.
Allowing your needle to cool for 10 seconds?
Right after face the needle up squirt 2 drops out and the needle immediately cools then inoculate
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,357
Loc: The Inexpressible...
Last seen: 13 minutes, 5 seconds
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Re: Okay, now I'm pissed. [Re: rudeboi]
#22461413 - 11/01/15 10:13 AM (8 years, 2 months ago) |
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Good point!
Maybe hes cooking the spores within the syringe, then cooking them some more in the super-heated PCed jars.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Okay, now I'm pissed. [Re: rudeboi] 1
#22461418 - 11/01/15 10:14 AM (8 years, 2 months ago) |
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Agar, agar, agar! 
Sorry, I am still in concert mode, but seriously after multiple successful grows and multiple failures... try something new. Limit the likelihood on contamination and give agar a try. It's not hard!! I promise.
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DrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
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Re: Okay, now I'm pissed. [Re: rudeboi]
#22461464 - 11/01/15 10:25 AM (8 years, 2 months ago) |
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I never wait either just squirt (course dont count that first bit as viable). Others spray away first.
Do you have pic's of what you thought was cobweb? Some times myc looks pretty fluffy/fuzzy when it starts especially from MS.
Also how long did you wait to determine that the cakes that did nothing werent going to do anything? I've had MS take quite a while to start. It easy to see people saying they had whole jars done in just a few weeks, this isnt always the case. It can take this amount a time just for the spore to germinate.
Edit: I see now a week. It can often take a couple weeks.
Edited by DrCrumbs (11/01/15 10:26 AM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
Arctic W. Fox said:
Quote:
Inocuole said: Why are you doing 1:1:3 as the cake ratios? It's 1:1:2.
Other than that, why are you still doing cakes after 5 successful grows? Maybe they're telling you to move on.
On an original tec I wrote down years ago, it called for 3c water, 3c BRF, 9c Verm. It worked so I stay with it.
Cakes worked, too, so I stay with them. I would love to have a whole garage filled with tubs filled with love, but I'm not there just yet. lol
I can try 1:1:2 on the next ones.
I am also wanting to build a SAB because I don't trust this new place I'm living in - I suspect a past flooding event and may have more contaminants floating around than my last home. :/
Doesn't Verm get wet anyway from the PC steam?
1 tub is enough to trump all the cakes you have done so far if they all fruited. You have a PC thats what stops most people from doing grains. By my calculations if you are doing 12 pints of brf if you filled those with grains instead you can easily fill a 30lt tub with 4 inches of substrate. All you have to buy is coir and casing soil to start out. And of course the tub. Dont even have to do hpoo or gypsum to start out. Just to see if you like it. Dont be intimated by tubs and agar they are awesome and fun to work with. I can't wait to see how my first monotub does.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Supalemonhaze said:
Quote:
Psilosoulful said:
Quote:
Supalemonhaze said:
Quote:
Psilosoulful said: Make sure to use that SAB. I hope you weren't inoculating in open air, that would explain these issues. Still air is your friend. 
I use LC in open air. Its impossible in my SAB to suck up mycelium from a jar. I could inoculate my grains in it but since I have to switch between working in and out I just do everything open air. Unless its agar of course. I will in the near future (hopefully) make some ports but since I've been so busy lately I do the stuff that requires my immediate attention like transfers and building shit for cultivation ( SGFC was a nightmare to finish. Didn't realise how much time it takes to do all those holes )
Why is it impossible to suck up the mycelium from the LC jar, is your SAB not big enough to be able to maneuver around. I use a 66qt tub and it has plenty of room, but I might wanna upgrade when I get into G2G'ing multiple jars.
Mine is 50litre ( only one I could find with a semi straight lid) but the size isnt really the problem is the visibility. I end up sucking up honey water only lol. And the tek I followed gave me the honey to water ratio in a few hundred ml's and I didn't think that it would be too little since it was my first LC, also it was a big ass round olive jar I used. So I have to tilt it and see where all the mycelium is which proved rather difficult in my sab.
Yesterday I made 2 more lc's that hopefully wont have this problem. Used pint jars this time and filled them to the top line just under the curve of the mouth. So yes I will definitely give my sab another chance once these colonise a bit.
EDIT: It would be nice to find a bigger container for a better sab but it seems like here noone makes shit simple anymore. No no no the lids have to have all those ups downs ridges and curves and all the colours of the rainbow. They just fucking have to. Everytime I see a pic of a sab with a clear lid on this site I drool lmao
I always wipe the inside top of my SAB with a dry paper towel after the box has been sprayed with soap water and allowed to sit for at least 5 minutes. This way I have clear visibility of what I'm working on.
Your SAB doesn't have to have a clear lid, that's only for monos. Just flip the tub over and you can see through the bottom of the tub.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Technically monos don't need clear lids either, just clear sides. Plenty of light comes in from the side and if you stack them you are blocking out the light from the top anyway. My first 4 have clear lids, second 4 do not. I don't notice any difference.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Okay, now I'm pissed. [Re: Kalistis]
#22461551 - 11/01/15 10:42 AM (8 years, 2 months ago) |
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That's true I've seen many cultivators with stacked monotubs and they pull off some amazing grows. I guess a strong clone/ isolate will help with that too!!
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Okay, now I'm pissed. [Re: Kalistis]
#22461577 - 11/01/15 10:48 AM (8 years, 2 months ago) |
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Quote:
Psilosoulful said:
I always wipe the inside top of my SAB with a dry paper towel after the box has been sprayed with soap water and allowed to sit for at least 5 minutes. This way I have clear visibility of what I'm working on.
Your SAB doesn't have to have a clear lid, that's only for monos. Just flip the tub over and you can see through the bottom of the tub. 
Yes thats why I was ranting about lids not being straight. I can live with a coloured lid but curves and ridges are a PITA. My sab has ridges that drop a few mm's but I can work with that at least. My agar is always slightly slanted because of this.
Quote:
Kalistis said: Technically monos don't need clear lids either, just clear sides. Plenty of light comes in from the side and if you stack them you are blocking out the light from the top anyway. My first 4 have clear lids, second 4 do not. I don't notice any difference.
Yes even mine isn't clear. I read that as long as light reaches the sides it should be ok. Theoretically it makes sense since the sides will be all wet and the small droplets will redirect the light everywhere.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Sure, good genetics helps any grow, but my MS grows are doing well with opaque lids. I have them stacked 4 high. Plenty of light comes in from the sides if you use clear tubs. Unless you are doing a closet grow, only have one tub, have to put the light source on top, or have inadequate light in the first pace then I see the value of clear lids. Otherwise, I don't.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Okay, now I'm pissed. [Re: Kalistis]
#22461611 - 11/01/15 10:58 AM (8 years, 2 months ago) |
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As far as my SAB, I looked for the largest container I could find with a flat lid. I found this (I think 108 qt) container at Home Depot. It has a nice flat lid. I think the larger the container, the better the lid lays. I made the mistake of cutting my arm holes as if the lid was my window, but luckily it still works.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Okay, now I'm pissed. [Re: Kalistis]
#22461614 - 11/01/15 10:58 AM (8 years, 2 months ago) |
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Quote:
Kalistis said: As far as my SAB, I looked for the largest container I could find with a flat lid. I found this (I think 108 qt) container at Home Depot. It has a nice flat lid. I think the larger the container, the better the lid lays. I made the mistake of cutting my arm holes as if the lid was my window, but luckily it still works.

Dem legs doe.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Okay, now I'm pissed. [Re: Inocuole]
#22461725 - 11/01/15 11:25 AM (8 years, 2 months ago) |
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Funguy24
Stranger

Registered: 11/01/15
Posts: 33
Last seen: 1 year, 5 months
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my first 2 attempts with PF TEK failed, 3rd attempt was successful but the yield was barely worth the trouble for me, im not dissing cakes as alot of people love them! personally id go SPORES>AGAR>GRAIN>BULK TRAYS, worked first time, no hassle
Edited by Funguy24 (11/01/15 01:05 PM)
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Okay, now I'm pissed. [Re: Inocuole] 1
#22462897 - 11/01/15 03:42 PM (8 years, 2 months ago) |
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Quote:
Inocuole said:
Quote:
Kalistis said: As far as my SAB, I looked for the largest container I could find with a flat lid. I found this (I think 108 qt) container at Home Depot. It has a nice flat lid. I think the larger the container, the better the lid lays. I made the mistake of cutting my arm holes as if the lid was my window, but luckily it still works.

Dem legs doe.
Here you go freak.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Okay, now I'm pissed. [Re: Kalistis] 1
#22462959 - 11/01/15 03:58 PM (8 years, 2 months ago) |
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Okay, now I'm pissed. [Re: azur]
#22463120 - 11/01/15 04:38 PM (8 years, 2 months ago) |
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Okay, now I'm pissed. [Re: Kalistis]
#22463222 - 11/01/15 05:01 PM (8 years, 2 months ago) |
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Quote:
Kalistis said:
Quote:
Inocuole said:
Quote:
Kalistis said: As far as my SAB, I looked for the largest container I could find with a flat lid. I found this (I think 108 qt) container at Home Depot. It has a nice flat lid. I think the larger the container, the better the lid lays. I made the mistake of cutting my arm holes as if the lid was my window, but luckily it still works.

Dem legs doe.
Here you go freak.

Actual women in cultivation are rare. Its not that we never seen women before its just most of us are complete nerds.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Okay, now I'm pissed. [Re: Pastywhyte]
#22464085 - 11/01/15 08:03 PM (8 years, 2 months ago) |
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Lmao thanks for making me laugh so early in the morning made my day... until I get to work that is.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Haha. I figured Inoc needed a little help. I didn't want him to strain his eyes trying to check my legs out through the reflection.
Perves.
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