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OfflineArctic W. Fox
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Registered: 09/23/14
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Okay, now I'm pissed.
    #22461130 - 11/01/15 09:09 AM (8 years, 2 months ago)

Third syringe, third attempt at jarred BRF tec. Third fail.

All three syringes from the same sponsor, bought at the same time. One grew black mold in all 12 jars. One didn't do anything at all in all 12 jars. This last one, I now see cobweb mold growing in all 12 jars.

I used the exact same tec on all of them, didn't change anything from what was successful in my previous grows.

What in the blue fuck is the issue here? I'm thinking this sponsor got lazy or an old batch of something and decided to sell it to me.


Any suggestions from the pros here?


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InvisibleMad Season
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox] * 1
    #22461139 - 11/01/15 09:11 AM (8 years, 2 months ago)

It's time to rage :wtfsonic:


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InvisibleBig Bear
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox]
    #22461143 - 11/01/15 09:13 AM (8 years, 2 months ago)

Tell us your tek?  Are you using a SAB? Are you flame sterilizing between inoculations?


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Invisibleazur
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox] * 1
    #22461147 - 11/01/15 09:13 AM (8 years, 2 months ago)

Agar ftw


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InvisibleShroomerInTheRye
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox]
    #22461149 - 11/01/15 09:14 AM (8 years, 2 months ago)

This same thing happened to me and a syringe of Lizard King.  Nothing grew except a stink to melt your eyeballs off.  Got it from a sponsor as well.  :mad2:


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Offlinerudeboi
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox]
    #22461150 - 11/01/15 09:14 AM (8 years, 2 months ago)

Have you tried one of them on agar?

How are your sterile techniques? I have recently changed mine after 4 runs running at a 40% success rate.

MS syringes are such a hit or miss:mad2:


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InvisibleSupalemonhaze
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Re: Okay, now I'm pissed. [Re: azur]
    #22461157 - 11/01/15 09:15 AM (8 years, 2 months ago)

Quote:

azur said:
Agar ftw





:whathesaid:


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OfflinePsilosoulful

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Re: Okay, now I'm pissed. [Re: Arctic W. Fox] * 1
    #22461164 - 11/01/15 09:17 AM (8 years, 2 months ago)

Make sure to use that SAB. I hope you weren't inoculating in open air, that would explain these issues. Still air is your friend. :smile:


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InvisibleInocuole
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox]
    #22461200 - 11/01/15 09:28 AM (8 years, 2 months ago)

Quote:

Arctic W. Fox said:
Third syringe, third attempt at jarred BRF tec. Third fail.


I used the exact same tec on all of them, didn't change anything from what was successful in my previous grows.





Uhhh.... I feel like I see a correlation here.


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OfflineArctic W. Fox
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox]
    #22461236 - 11/01/15 09:40 AM (8 years, 2 months ago)

  • 12 wide-mouth half-pint jars.
  • Four holes drilled in the lids, equally spaced.
  • Mixed 1-part water, 1-part organic BRF, 3-parts Verm.
  • 1/2 cup of fluffed mixture in each jar, 4tsp of dry Verm on top.
  • Placed lid upside-down and secured on jars.
  • Cut aluminium foil to size and covered the lid.
  • Place jars inside PC, stacked two high.
  • Placed open spring-water-filled pickle jar in PC to space BRF jars.
  • Filled PC with spring water about half way up bottom BRF jars.
  • Closed PC, added 15psi weights, turned on heat to high until steam, brought down heat until the familiar pst...pst...pst...
  • Pressure cook for 2 hours. Turn off heat. Let set for 24 hours.
  • Remove weights, remove PC lid.
  • Syringe at room temperature and shaken (not stirred).
  • Carefully remove foil from one jar, flame sterilize needle with lighter, wait 10 seconds to cool, inoculate through each drilled holes at full needle depth with about 1/4cc (almost 1cc of fluid per jar). Replaced foil. Set inside cabinet shelf.
  • Repeat.


Any fluid left in the syringe at the end of 12 jars gets dumped into the last jar.

I check back a week later and find nothing useful growing and have to toss unopened/contaminated jars.

I have done this five other times with 100% success. These last three times, I got nada.


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InvisibleInocuole
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox]
    #22461249 - 11/01/15 09:42 AM (8 years, 2 months ago)

Quote:

Arctic W. Fox said:
  • 12 wide-mouth half-pint jars.
  • Four holes drilled in the lids, equally spaced.
  • Mixed 1-part water, 1-part organic BRF, 3-parts Verm. 
  • 1/2 cup of fluffed mixture in each jar, 4tsp of dry Verm on top.
  • Placed lid upside-down and secured on jars.
  • Cut aluminium foil to size and covered the lid.
  • Place jars inside PC, stacked two high.
  • Placed open spring-water-filled pickle jar in PC to space BRF jars.
  • Filled PC with spring water about half way up bottom BRF jars.
  • Closed PC, added 15psi weights, turned on heat to high until steam, brought down heat until the familiar pst...pst...pst...
  • Pressure cook for 2 hours. Turn off heat. Let set for 24 hours.
  • Remove weights, remove PC lid.
  • Syringe at room temperature and shaken (not stirred).
  • Carefully remove foil from one jar, flame sterilize needle with lighter, wait 10 seconds to cool, inoculate through each drilled holes at full needle depth with about 1/4cc (almost 1cc of fluid per jar). Replaced foil. Set inside cabinet shelf.
  • Repeat.


Any fluid left in the syringe at the end of 12 jars gets dumped into the last jar.

I check back a week later and find nothing useful growing and have to toss unopened/contaminated jars.

I have done this five other times with 100% success.




Why are you doing 1:1:3 as the cake ratios?  It's 1:1:2.

Other than that, why are you still doing cakes after 5 successful grows?  Maybe they're telling you to move on.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleSupalemonhaze
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Re: Okay, now I'm pissed. [Re: Psilosoulful]
    #22461271 - 11/01/15 09:46 AM (8 years, 2 months ago)

Quote:

Psilosoulful said:
Make sure to use that SAB. I hope you weren't inoculating in open air, that would explain these issues. Still air is your friend. :smile:




I use LC in open air. Its impossible in my SAB to suck up mycelium from a jar. I could inoculate my grains in it but since I have to switch between working in and out I just do everything open air. Unless its agar of course. I will in the near future (hopefully) make some ports but since I've been so busy lately I do the stuff that requires my immediate attention like transfers and building shit for cultivation ( SGFC was a nightmare to finish. Didn't realise how much time it takes to do all those holes  :wowz:)


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Okay, now I'm pissed. [Re: Inocuole]
    #22461277 - 11/01/15 09:47 AM (8 years, 2 months ago)

Hey OP you still didn't mention if you used a SAB or not...and don't replace the foil after inoculating, that'll only trap moisture on the jar lid and lead to bacterial growth that could easily get into your jars through the exposed holes. Once that verm barrier gets wet, it's bad news for your cakes...


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OfflinePsilosoulful

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Re: Okay, now I'm pissed. [Re: Supalemonhaze]
    #22461296 - 11/01/15 09:51 AM (8 years, 2 months ago)

Quote:

Supalemonhaze said:
Quote:

Psilosoulful said:
Make sure to use that SAB. I hope you weren't inoculating in open air, that would explain these issues. Still air is your friend. :smile:




I use LC in open air. Its impossible in my SAB to suck up mycelium from a jar. I could inoculate my grains in it but since I have to switch between working in and out I just do everything open air. Unless its agar of course. I will in the near future (hopefully) make some ports but since I've been so busy lately I do the stuff that requires my immediate attention like transfers and building shit for cultivation ( SGFC was a nightmare to finish. Didn't realise how much time it takes to do all those holes  :wowz:)



Why is it impossible to suck up the mycelium from the LC jar, is your SAB not big enough to be able to maneuver around. I use a 66qt tub and it has plenty of room, but I might wanna upgrade when I get into G2G'ing multiple jars.


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InvisibleInocuole
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Re: Okay, now I'm pissed. [Re: Psilosoulful]
    #22461299 - 11/01/15 09:51 AM (8 years, 2 months ago)

108qt is adequate.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineArctic W. Fox
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Re: Okay, now I'm pissed. [Re: Inocuole]
    #22461310 - 11/01/15 09:52 AM (8 years, 2 months ago)

Quote:

Inocuole said:
Why are you doing 1:1:3 as the cake ratios?  It's 1:1:2.

Other than that, why are you still doing cakes after 5 successful grows?  Maybe they're telling you to move on.




On an original tec I wrote down years ago, it called for 3c water, 3c BRF, 9c Verm. It worked so I stay with it.

Cakes worked, too, so I stay with them. I would love to have a whole garage filled with tubs filled with love, but I'm not there just yet. lol

I can try 1:1:2 on the next ones.

I am also wanting to build a SAB because I don't trust this new place I'm living in - I suspect a past flooding event and may have more contaminants floating around than my last home. :/

Doesn't Verm get wet anyway from the PC steam?


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Offlinetetherface
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Re: Okay, now I'm pissed. [Re: Inocuole]
    #22461311 - 11/01/15 09:52 AM (8 years, 2 months ago)

the vendor will/should make it right with you ive had similar results from a non sponsor and even they made it right I may have had to pay shipping I don't really remember as its been awhile


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InvisibleSupalemonhaze
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Re: Okay, now I'm pissed. [Re: Inocuole] * 1
    #22461336 - 11/01/15 09:56 AM (8 years, 2 months ago)

Quote:

Inocuole said:

Why are you doing 1:1:3 as the cake ratios?  It's 1:1:2.

Other than that, why are you still doing cakes after 5 successful grows?  Maybe they're telling you to move on.




I second this. Grains are so much faster. I did grains first and then made pf cakes a few days later. Had to throw away grains due to stalling (bacteria probably), so I did grains again. I just spawned those grains 3 days ago in a monotub while my pf cakes are still consolidating and some are in the end of colonisation. I will never do pf again probably. The pf jars were pints and grains are quarts so you can see why in not that interested in cakes. Also you can spawn grains to at least double what you have in your jars. Hell if I mentioned all the reasons why you should switch to grains I'd probably be here all night


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OfflineLogicaL ChaosM
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Re: Okay, now I'm pissed. [Re: Arctic W. Fox]
    #22461357 - 11/01/15 10:01 AM (8 years, 2 months ago)

WHHHHHYYYY! :wtfsonic:

The only thing i can think of is maybe the jars are still too hot after 24 hours and you are killing your spores when you inject them into the jar.

You should see SOME growth. But none is very strange.

ive  accidently cooked my spores before from forgeting to let the jars cool. I got lots of infections. Learned my lesson real quick :nonono:

Maybe try cooling the jars for 48 Hours or use a regular pot for boiling water instead of PCing them at super high temps :shrug:


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InvisibleSupalemonhaze
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Re: Okay, now I'm pissed. [Re: Psilosoulful]
    #22461386 - 11/01/15 10:08 AM (8 years, 2 months ago)

Quote:

Psilosoulful said:
Quote:

Supalemonhaze said:
Quote:

Psilosoulful said:
Make sure to use that SAB. I hope you weren't inoculating in open air, that would explain these issues. Still air is your friend. :smile:




I use LC in open air. Its impossible in my SAB to suck up mycelium from a jar. I could inoculate my grains in it but since I have to switch between working in and out I just do everything open air. Unless its agar of course. I will in the near future (hopefully) make some ports but since I've been so busy lately I do the stuff that requires my immediate attention like transfers and building shit for cultivation ( SGFC was a nightmare to finish. Didn't realise how much time it takes to do all those holes  :wowz:)



Why is it impossible to suck up the mycelium from the LC jar, is your SAB not big enough to be able to maneuver around. I use a 66qt tub and it has plenty of room, but I might wanna upgrade when I get into G2G'ing multiple jars.




Mine is 50litre ( only one I could find with a semi straight lid) but the size isnt really the problem is the visibility. I end up sucking up honey water only lol. And the tek I followed gave me the honey to water ratio in a few hundred ml's and I didn't think that it would be too little since it was my first LC, also it was a big ass round olive jar I used. So I have to tilt it and see where all the mycelium is which proved rather difficult in my sab.

Yesterday I made 2 more lc's that hopefully wont have this problem. Used pint jars this time and filled them to the top line just under the curve of the mouth. So yes I will definitely give my sab another chance once these colonise a bit.

EDIT: It would be nice to find a bigger container for a better sab but it seems like here noone makes shit simple anymore. No no no the lids have to have all those ups downs ridges and curves and all the colours of the rainbow. They just fucking have to. Everytime I see a pic of a sab with a clear lid on this site I drool lmao


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