|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
|
|
I've never changed my surgical tape on the plates, keep using the same paper towels, lids are super loose, etc. My contam rates have not gone up.
|
taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
|
|
I don't even use the paper towels anymore 
I think we had this discussion in this thread already between me, Muda and a few others. We all re-use the same foil, don't wash our plates, don't use paper towel and don't change our micropore.
Pretty much set-and-forget tek
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 17 days, 10 hours
|
|
Quote:
Pastywhyte said: Exactly guys. Those lids get better with use. Funny how the things that worry the new people are what the vets prefer 
Yeah I haven't change my micropore in a long time either. Sometimes I will stick a new piece on overtop, but thats it.
Yea dude at first I was like "man these lids are kinda tight" then "man these are super loose did I fuck them up" and now Im like "man I kinda like them better loose it was too clumbsy before"
Gotta remember not to pick them up by the lid though.
--------------------
 Amateur Mycologists United AMU Q&A
|
pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,178
Loc: rural ghetto
|
Re: Pasty Agar Tek [Re: mushpunx]
#22460428 - 11/01/15 03:00 AM (8 years, 3 months ago) |
|
|
...Uninoculated plates got contaminated from (I suppose)adjacent contaminated plates(forgot to get rid of)... micropore doesn't provide enough barrier for such a situation... have you guys any experience with this or is it just me? I'll stuff the holes with a small polyfill next time around..
--------------------
Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
Edited by pablokabute (11/01/15 08:31 AM)
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
It's never been a problem for me. Moste of my plate have poly-fill or SFD on them now though.
|
blackdust


Registered: 02/28/09
Posts: 8,327
|
Re: Pasty Agar Tek [Re: MudaFuka]
#22460852 - 11/01/15 08:01 AM (8 years, 3 months ago) |
|
|
33 days old 
|
HonkyGeorge
Invest in Franquito Industries

Registered: 11/02/15
Posts: 33
Last seen: 6 years, 6 months
|
|
I just got a pressure cooker today--can't wait to get serious with this. Does micropore only keep the glad rounds from being smushed? Would 2 layers limit gas flow, or am I being paranoid? Also, I dug up a first aid kit that has what looks like micropore tape. Would that pop up in one of those kits?
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
|
|
yep that would "pop up" in one of those lol, it's there because the hole stops a vacuum from forming so the hole needs to be filtered, 2 layers won't be a huge issue
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
|
Re: Pasty Agar Tek [Re: cronicr]
#22483925 - 11/05/15 11:24 PM (8 years, 3 months ago) |
|
|
I have put as many as 4 on it. 2 is best IMO
|
HonkyGeorge
Invest in Franquito Industries

Registered: 11/02/15
Posts: 33
Last seen: 6 years, 6 months
|
|
Does it matter if the potato flakes are flavored or not? I got butter homestyle by mistake.
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
|
Re: Pastywhyte's "Easy Agar" Tek [Re: HonkyGeorge]
#22517970 - 11/13/15 12:28 PM (8 years, 3 months ago) |
|
|
I believe I asked Pasty about that last time because I had garlic flakes. IIRC, yes, it does matter, you want regular, plain potato flakes. Otherwise there's too much salt in the mixture.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
|
|
Check the nutritional content. If it has sodium I would avoid it for sure.
|
HonkyGeorge
Invest in Franquito Industries

Registered: 11/02/15
Posts: 33
Last seen: 6 years, 6 months
|
|
Yeah, I just checked the back of the bag...the mycillium would not enjoy any of that. Thanks.
|
PreparationH
apply daily


Registered: 03/28/05
Posts: 18,335
Loc: Amsterdam
Last seen: 22 hours, 11 minutes
|
|
Saved... for next project
|
globos
Regular User


Registered: 04/09/10
Posts: 658
|
|
I've grown lots of different plants with average to good success rate, but I'm a total newbie at making my own mushrooms.
Always wanted to do agar. Now I've got 8 containers (Pastywhyte style) in the fridge and one ready-made growbox is fruiting. How do I go about cloning? Just drop a piece of mushroom over the agar? And which tek would you advise from then onwards? There are so many I'm baffled.
Thanks, as usual, for the helpful hands.
|
taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
|
Re: Pasty Agar Tek [Re: globos]
#22594360 - 11/30/15 11:57 AM (8 years, 2 months ago) |
|
|
You gotta take a clone brotha,
Open it up with your hands and then take a transfer inside, don't cut straight into the thing with a flamed scalpel because you'll push contams in. The theory is the outside of the mushroom, which was fruited in open air, will be covered with contams while the inside will not.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
|
BlueIndian
Maestro



Registered: 01/17/10
Posts: 858
|
Re: Pasty Agar Tek [Re: taGyo]
#22594430 - 11/30/15 12:14 PM (8 years, 2 months ago) |
|
|
After being intimidated for way too long I started some pastyplates! Having a hard time getting anything to start by using a drop from a syringe even though I know it's viable. Other plates either have a colonised grain in each one or a small piece of tissue cloned...all of those are rocking! 
So what am I looking for to transfer with? Faster rhizo growth? Not getting much of that but it is all spreading nicely. When transferring is it a problem by cutting down into the agar to cut out the myc? And last...is 2 transfers enough to consider it an isolate?
Oh...and if going to store plates in the fridge do you want the entire surface colonized before storing?
|
taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
|
|
Rhizo is overrated. In fact everything is overrated until fruiting potential is locked down. I whip up nutrient heavy agar. A tad heavier then Pasty's formula, which is already a little high btw, and this favors tomentose growth. All of my agar is tomentose and my tubs come out great. I prefer the slower growth, it gives me time to remember they're there 
Transfer from the leading edge brotha,
http://www.shroomery.org/forums/upload.php?action=viewthumb&screen=3
In that first wedge, the top most from like 9 o'clock to 3 o'clock, I'd transfer from closer to three o'clock, see the quicker genetics expanding out faster then the other sides?
That's isolating for speed, the only thing we can see on agar.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
|
Munchauzen


Registered: 06/22/11
Posts: 14,343
|
|
Quote:
BlueIndian said: After being intimidated for way too long I started some pastyplates! Having a hard time getting anything to start by using a drop from a syringe even though I know it's viable. Other plates either have a colonised grain in each one or a small piece of tissue cloned...all of those are rocking! 
So what am I looking for to transfer with? Faster rhizo growth? Not getting much of that but it is all spreading nicely. When transferring is it a problem by cutting down into the agar to cut out the myc? And last...is 2 transfers enough to consider it an isolate?
Oh...and if going to store plates in the fridge do you want the entire surface colonized before storing?
just transfer the fastest growing areas. being rhizo or not doesn't really matter for anything, just looks.
cut into the agar. this is why we refer to agar transfers as "agar wedges" because the mycelium will recoup faster on the established agar than having to find a new food source.
2 transfers will probably make it clean. 2 transfers is generally not enough to find a monoculture. Some people have done up to a dozen transfers before finding the monoculture.
Do you need to find a monoculture? No.
Can you grow buttloads of mushrooms with multispore grows and clone grows? Yes.
Since you are new, I would recommend not thinking about isolates. Like I mentioned, you may have to do a lot of transfers to get to the monoculture, and even then its not guaranteed to even grow anything. So not only do you have to make a bunch of transfers, you have to test all those transfers for viability. Its fucking lengthy, grueling process.
|
BlueIndian
Maestro



Registered: 01/17/10
Posts: 858
|
|
Well yeah the goal was to start trying to get some isolate or monocultures in order to achieve consistent flushes and potency. I was under the impression 3 transfers should result in an isolate. IMO no real point in agar work if not working towards an isolate....may as well stick with G2G transfers if it's MS. All I know is I'm pleased the first agar attempts have gone well. It's not a variety I plan to keep around so it's been more of practice. Just one of those things I've been too scared to even attempt...out of the 8 or so I've made no contams that I can see.
|
|